ButhA.17230.a

Pyruvate dehydrogenase complex, E1 component, pyruvate dehydrogenase beta subunit

CENTER ID: ButhA.17230.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.17230.a.A1.GE32078 Full length( ButhA.17230.a ) 1 324
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.1044
RefSeq: YP_439126.1
UniProt: Q2T6S3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIRRLTYREA LRDALRDALS NDPRVFLMGE DVGRYGGSYA VSAGLLEAFG PERVRDTPLS ELAFTGMGIG AALGGMRPIV EIMTVNFSLL ALDQIVNTAA LYHHMSGGQF SVPLVIRMAT GAGRQVAAQH SHSFEGWYAG IPGIKVLAPA TVEDARHMLA PALADPDPVL IFEHAGLYNM EGDLSAMTSV DIRSAKVRRD GGDVAILAYG GSLPKALEAA DALAGEGISA EVVDLRVLRP LDDATIMASV IKCRRAVIVD ECWRSASVAS EIVARIVEQA FYELDAPLAR VCAEDVPIPY ARHMEEAALP QVDKIVAAVK QLLS
NT Sequence
GTGATCAGGC GGCTCACCTA CCGCGAGGCG CTGCGCGACG CGTTGCGCGA CGCGCTGTCG AACGATCCGC GCGTGTTCCT GATGGGCGAG GACGTGGGGC GCTACGGCGG CAGCTACGCG GTGTCGGCGG GCTTGCTGGA AGCGTTCGGT CCCGAGCGCG TGCGCGACAC GCCGCTGTCG GAGCTTGCGT TCACAGGCAT GGGAATCGGC GCGGCGCTGG GCGGCATGCG GCCGATCGTC GAGATCATGA CGGTCAACTT CAGCCTGCTC GCGCTCGACC AGATCGTCAA CACCGCCGCG CTCTACCATC ACATGTCCGG CGGGCAATTC TCGGTGCCGC TCGTGATCCG CATGGCCACG GGCGCGGGCC GCCAGGTCGC GGCGCAGCAC TCGCACAGCT TCGAGGGCTG GTATGCCGGC ATTCCGGGCA TCAAGGTGCT CGCGCCCGCG ACCGTCGAAG ACGCGCGCCA CATGCTGGCG CCGGCGCTCG CCGATCCCGA TCCGGTGCTG ATCTTCGAGC ATGCGGGCCT CTACAACATG GAAGGCGACT TGTCCGCGAT GACGAGCGTC GACATCCGGT CGGCGAAGGT GAGGCGCGAC GGCGGGGACG TCGCGATTCT CGCGTACGGC GGCTCGTTGC CGAAGGCGCT CGAGGCGGCC GATGCGCTTG CGGGCGAAGG CATCTCGGCC GAGGTCGTCG ATCTGCGCGT GCTGCGCCCG CTCGACGACG CGACGATCAT GGCTTCGGTG ATCAAGTGCC GGCGTGCGGT GATCGTCGAC GAATGCTGGC GCAGCGCGAG CGTCGCGTCG GAAATCGTCG CGCGCATCGT CGAACAGGCA TTCTACGAAC TCGATGCGCC GCTCGCGCGC GTGTGCGCCG AGGACGTGCC GATTCCGTAT GCCCGGCACA TGGAAGAAGC GGCGTTGCCC CAGGTCGACA AGATCGTCGC CGCGGTGAAG CAATTGCTTT CG
Details for ButhA.17230.a.A1.GE32078
HARVESTED ON: 4/19/2011
SEQUENCED ON: 4/25/2011
EXPECTED MW: 37kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMVIRRLT YREALRDALR DALSNDPRVF LMGEDVGRYG GSYAVSAGLL EAFGPERVRD TPLSELAFTG MGIGAALGGM RPIVEIMTVN FSLLALDQIV NTAALYHHMS GGQFSVPLVI RMATGAGRQV AAQHSHSFEG WYAGIPGIKV LAPATVEDAR HMLAPALADP DPVLIFEHAG LYNMEGDLSA MTSVDIRSAK VRRDGGDVAI LAYGGSLPKA LEAADALAGE GISAEVVDLR VLRPLDDATI MASVIKCRRA VIVDECWRSA SVASEIVARI VEQAFYELDA PLARVCAEDV PIPYARHMEE AALPQVDKIV AAVKQLLS
Validated NT Sequence
ttacgaaagc aattgcttca ccgcggcgac gatcttgtcg acctggggca acgccgcttc ttccatgtgc cgggcatacg gaatcggcac gtcctcggcg cacacgcgcg cgagcggcgc atcgagttcg tagaatgcct gttcgacgat gcgcgcgacg atttccgacg cgacgctcgc gctgcgccag cattcgtcga cgatcaccgc acgccggcac ttgatcaccg aagccatgat cgtcgcgtcg tcgagcgggc gcagcacgcg cagatcgacg acctcggccg agatgccttc gcccgcaagc gcatcggccg cctcgagcgc cttcggcaac gagccgccgt acgcgagaat cgcgacgtcc ccgccgtcgc gcctcacctt cgccgaccgg atgtcgacgc tcgtcatcgc ggacaagtcg ccttccatgt tgtagaggcc cgcatgctcg aagatcagca ccggatcggg atcggcgagc gccggcgcca gcatgtggcg cgcgtcttcg acggtcgcgg gcgcgagcac cttgatgccc ggaatgccgg cataccagcc ctcgaagctg tgcgagtgct gcgccgcgac ctggcggccc gcgcccgtgg ccatgcggat cacgagcggc accgagaatt gcccgccgga catgtgatgg tagagcgcgg cggtgttgac gatctggtcg agcgcgagca ggctgaagtt gaccgtcatg atctcgacga tcggccgcat gccgcccagc gccgcgccga ttcccatgcc tgtgaacgca agctccgaca gcggcgtgtc gcgcacgcgc tcgggaccga acgcttccag caagcccgcc gacaccgcgt agctgccgcc gtagcgcccc acgtcctcgc ccatcaggaa cacgcgcgga tcgttcgaca gcgcgtcgcg caacgcgtcg cgcagcgcct cgcggtaggt gagccgcctg atcaccatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tnntganc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVIRRLTYR EALRDALRDA LSNDPRVFLM GEDVGRYGGS YAVSAGLLEA FGPERVRDTP LSELAFTGMG IGAALGGMRP IVEIMTVNFS LLALDQIVNT AALYHHMSGG QFSVPLVIRM ATGAGRQVAA QHSHSFEGWY AGIPGIKVLA PATVEDARHM LAPALADPDP VLIFEHAGLY NMEGDLSAMT SVDIRSAKVR RDGGDVAILA YGGSLPKALE AADALAGEGI SAEVVDLRVL RPLDDATIMA SVIKCRRAVI VDECWRSASV ASEIVARIVE QAFYELDAPL ARVCAEDVPI PYARHMEEAA LPQVDKIVAA VKQLLS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgatcagg cggctcacct accgcgaggc gctgcgcgac gcgttgcgcg acgcgctgtc gaacgatccg cgcgtgttcc tgatgggcga ggacgtgggg cgctacggcg gcagctacgc ggtgtcggcg ggcttgctgg aagcgttcgg tcccgagcgc gtgcgcgaca cgccgctgtc ggagcttgcg ttcacaggca tgggaatcgg cgcggcgctg ggcggcatgc ggccgatcgt cgagatcatg acggtcaact tcagcctgct cgcgctcgac cagatcgtca acaccgccgc gctctaccat cacatgtccg gcgggcaatt ctcggtgccg ctcgtgatcc gcatggccac gggcgcgggc cgccaggtcg cggcgcagca ctcgcacagc ttcgagggct ggtatgccgg cattccgggc atcaaggtgc tcgcgcccgc gaccgtcgaa gacgcgcgcc acatgctggc gccggcgctc gccgatcccg atccggtgct gatcttcgag catgcgggcc tctacaacat ggaaggcgac ttgtccgcga tgacgagcgt cgacatccgg tcggcgaagg tgaggcgcga cggcggggac gtcgcgattc tcgcgtacgg cggctcgttg ccgaaggcgc tcgaggcggc cgatgcgctt gcgggcgaag gcatctcggc cgaggtcgtc gatctgcgcg tgctgcgccc gctcgacgac gcgacgatca tggcttcggt gatcaagtgc cggcgtgcgg tgatcgtcga cgaatgctgg cgcagcgcga gcgtcgcgtc ggaaatcgtc gcgcgcatcg tcgaacaggc attctacgaa ctcgatgcgc cgctcgcgcg cgtgtgcgcc gaggacgtgc cgattccgta tgcccggcac atggaagaag cggcgttgcc ccaggtcgac aagatcgtcg ccgcggtgaa gcaattgctt tcgtaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa