SSGCID: Target Page

ButhA.17085.a

Coproporphyrinogen-III oxidase, aerobic (Coprogen oxidase) (Coproporphyrinogenase) (EC 1.3.3.3) (BTH_I1013) (hemF)

CENTER ID:	ButhA.17085.a
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.17085.a.A1.GE32828	full length		1	326

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.17085.a.A1.PS01493	full length		1	326

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.3762
RefSeq:	YP_441567.1
UniProt:	Q2SZT2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPGRSIFRRR PSRGCRPTSM TDSTYDVNRV RAYLQGLQTR IADALGAFDG TPLSADAWQR SPGERLRGGG CTRILEEGGF FERAGIGFSD VAGDALPPSA SASRPQLAGR GFEALGVSLV LHPRNPYCPT VHMNVRMLIA TKPGEAPVFW FGGGMDLTPI YGFEEDARHF HRTCRAALDP FGAELYPRFK KWCDDYFFLK HRNEARGIGG IFFDDFSELG FERSFEMLQS VGDAFLPSYL PIVERRRDMP YGERERAFQT QRRGRYVEFN LVFDRGTLFG LQSGGRTESI LLSMPPTAGW RYDWHPAPDT PEARLYSDFL VPRDWA

NT Sequence

atgcccggca gaagcatttt tcgacggcgt ccgtcacgcg gatgccgccc cacatcgatg accgattcga cttacgacgt caaccgagta cgcgcgtacc tgcaagggct gcagacgcgc atagccgacg cgctcggcgc cttcgacggc acgccgcttt ccgccgacgc gtggcagcgc agccccggcg agcggctgcg cggcggcggc tgcacgcgca ttctcgagga aggcggcttc ttcgagcgcg cgggcatcgg cttttccgac gtcgcgggcg acgcgctgcc gccttcggcg agcgcgtcgc gcccgcagct cgccgggcgc ggcttcgagg cgctcggcgt gtcgctcgtg ctgcacccgc gcaacccgta ctgcccgacc gtccacatga acgtgcggat gctgatcgcg acgaagccgg gcgaggcgcc cgtgttctgg ttcggcggcg gcatggatct gacgccgatc tacggcttcg aagaggatgc gcggcacttc catcgcacgt gccgcgcggc gctcgatccg ttcggcgccg agctgtaccc gcgtttcaag aagtggtgcg acgattattt cttcctgaag caccgcaacg aagcgcgcgg catcggcggc atcttcttcg acgacttctc ggagctcggc ttcgaacgct cgttcgaaat gctgcaaagc gtcggcgacg cgttcctgcc gtcgtacctg ccgatcgtcg agcgccgccg cgacatgccg tacggcgagc gcgagcgcgc gttccagacg caacggcgcg gccgctacgt cgaattcaac ctggtgttcg accgcggcac gctgttcggg ctgcaaagcg gcggccgcac cgagtcgatc ctgctgtcga tgccgccgac ggcgggctgg cgctacgact ggcacccggc cccggacacg ccggaagccc gcctctacag cgatttcctc gtgccgcgcg actgggcatg a

Details for ButhA.17085.a.A1.GE32828

HARVESTED ON:	6/28/2011
SEQUENCED ON:	7/22/2011
EXPECTED MW:	39kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMPGRSIFRR RPSRGCRPTS MTDSTYDVNR VRAYLQGLQT RIADALGAFD GTPLSADAWQ RSPGERLRGG GCTRILEEGG FFERAGIGFS DVAGDALPPS ASASRPQLAG RGFEALGVSL VLHPRNPYCP TVHMNVRMLI ATKPGEAPVF WFGGGMDLTP IYGFEEDARH FHRTCRAALD PFGAELYPRF KKWCDDYFFL KHRNEARGIG GIFFDDFSEL GFERSFEMLQ SVGDAFLPSY LPIVERRRDM PYGERERAFQ TQRRGRYVEF NLVFDRGTLF GLQSGGRTES ILLSMPPTAG WRYDWHPAPD TPEARLYSDF LVPRDWA

Validated NT Sequence

tatgcccagt cgcgcggcac gaggaaatcg ctgtagaggc gggcttccgg cgtgtccggg gccgggtgcc agtcgtagcg ccagcccgcc gtcggcggca tcgacagcag gatcgactcg gtgcggccgc cgctttgcag cccgaacagc gtgccgcggt cgaacaccag gttgaattcg acgtagcggc cgcgccgttg cgtctggaac gcgcgctcgc gctcgccgta cggcatgtcg cggcggcgct cgacgatcgg caggtacgac ggcaggaacg cgtcgccgac gctttgcagc atttcgaacg agcgttcgaa gccgagctcc gagaagtcgt cgaagaagat gccgccgatg ccgcgcgctt cgttgcggtg cttcaggaag aaataatcgt cgcaccactt cttgaaacgc gggtacagct cggcgccgaa cggatcgagc gccgcgcggc acgtgcgatg gaagtgccgc gcatcctctt cgaagccgta gatcggcgtc agatccatgc cgccgccgaa ccagaacacg ggcgcctcgc ccggcttcgt cgcgatcagc atccgcacgt tcatgtggac ggtcgggcag tacgggttgc gcgggtgcag cacgagcgac acgccgagcg cctcgaagcc gcgcccggcg agctgcgggc gcgacgcgct cgccgaaggc ggcagcgcgt cgcccgcgac gtcggaaaag ccgatgcccg cgcgctcgaa gaagccgcct tcctcgagaa tgcgcgtgca gccgccgccg cgcagccgct cgccggggct gcgctgccac gcgtcggcgg aaagcggcgt gccgtcgaag gcgccgagcg cgtcggctat gcgcgtctgc agcccttgca ggtacgcgcg tactcggttg acgtcgtaag tcgaatcggt catcgatgtg gggcggcatc cgcgtgacgg acgccgtcga aaaatgcttc tgccgggcat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc catggtatnt ctcc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcccggcaga agcatttttc gacggcgtcc gtcacgcgga tgccgcccca catcgatgac cgattcgact tacgacgtca accgagtacg cgcgtacctg caagggctgc agacgcgcat agccgacgcg ctcggcgcct tcgacggcac gccgctttcc gccgacgcgt ggcagcgcag ccccggcgag cggctgcgcg gcggcggctg cacgcgcatt ctcgaggaag gcggcttctt cgagcgcgcg ggcatcggct tttccgacgt cgcgggcgac gcgctgccgc cttcggcgag cgcgtcgcgc ccgcagctcg ccgggcgcgg cttcgaggcg ctcggcgtgt cgctcgtgct gcacccgcgc aacccgtact gcccgaccgt ccacatgaac gtgcggatgc tgatcgcgac gaagccgggc gaggcgcccg tgttctggtt cggcggcggc atggatctga cgccgatcta cggcttcgaa gaggatgcgc ggcacttcca tcgcacgtgc cgcgcggcgc tcgatccgtt cggcgccgag ctgtacccgc gtttcaagaa gtggtgcgac gattatttct tcctgaagca ccgcaacgaa gcgcgcggca tcggcggcat cttcttcgac gacttctcgg agctcggctt cgaacgctcg ttcgaaatgc tgcaaagcgt cggcgacgcg ttcctgccgt cgtacctgcc gatcgtcgag cgccgccgcg acatgccgta cggcgagcgc gagcgcgcgt tccagacgca acggcgcggc cgctacgtcg aattcaacct ggtgttcgac cgcggcacgc tgttcgggct gcaaagcggc ggccgcaccg agtcgatcct gctgtcgatg ccgccgacgg cgggctggcg ctacgactgg cacccggccc cggacacgcc ggaagcccgc ctctacagcg atttcctcgt gccgcgcgac tgggcataaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa

Details for ButhA.17085.a.A1.PS01493

PURIFICATION DATe:	5/10/2012
CONCENTRATION:	19.58mg/ml
OBSERVED MW:	38kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	6
VIAL VOLUME:	110µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCCCGGCAGA AGCATTTTTC GACGGCGTCC GTCACGCGGA TGCCGCCCCA CATCGATGAC CGATTCGACT TACGACGTCA ACCGAGTACG CGCGTACCTG CAAGGGCTGC AGACGCGCAT AGCCGACGCG CTCGGCGCCT TCGACGGCAC GCCGCTTTCC GCCGACGCGT GGCAGCGCAG CCCCGGCGAG CGGCTGCGCG GCGGCGGCTG CACGCGCATT CTCGAGGAAG GCGGCTTCTT CGAGCGCGCG GGCATCGGCT TTTCCGACGT CGCGGGCGAC GCGCTGCCGC CTTCGGCGAG CGCGTCGCGC CCGCAGCTCG CCGGGCGCGG CTTCGAGGCG CTCGGCGTGT CGCTCGTGCT GCACCCGCGC AACCCGTACT GCCCGACCGT CCACATGAAC GTGCGGATGC TGATCGCGAC GAAGCCGGGC GAGGCGCCCG TGTTCTGGTT CGGCGGCGGC ATGGATCTGA CGCCGATCTA CGGCTTCGAA GAGGATGCGC GGCACTTCCA TCGCACGTGC CGCGCGGCGC TCGATCCGTT CGGCGCCGAG CTGTACCCGC GTTTCAAGAA GTGGTGCGAC GATTATTTCT TCCTGAAGCA CCGCAACGAA GCGCGCGGCA TCGGCGGCAT CTTCTTCGAC GACTTCTCGG AGCTCGGCTT CGAACGCTCG TTCGAAATGC TGCAAAGCGT CGGCGACGCG TTCCTGCCGT CGTACCTGCC GATCGTCGAG CGCCGCCGCG ACATGCCGTA CGGCGAGCGC GAGCGCGCGT TCCAGACGCA ACGGCGCGGC CGCTACGTCG AATTCAACCT GGTGTTCGAC CGCGGCACGC TGTTCGGGCT GCAAAGCGGC GGCCGCACCG AGTCGATCCT GCTGTCGATG CCGCCGACGG CGGGCTGGCG CTACGACTGG CACCCGGCCC CGGACACGCC GGAAGCCCGC CTCTACAGCG ATTTCCTCGT GCCGCGCGAC TGGGCATAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA