ButhA.17069.a
Putative uncharacterized protein
| CENTER ID: | ButhA.17069.a |
| ORGANISM: | Burkholderia thailandensis E264 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.17069.a.A2.GE34771 | Mature protein( ButhA.17069.a ) | 34 | 238 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.17069.a.A2.PS01486 | Mature protein( ButhA.17069.a ) | 34 | 238 |
Structures
6AR7
DEPOSITED: 8/21/2017
DETERMINATION: XRay
CLONE: ButhA.17069.a.A2.GE34771
PROTEIN: ButhA.17069.a.A2.PS38189
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_215020 |
| PATRIC ID: | fig|271848.6.peg.3584 |
| RefSeq: | YP_441418.1 |
| UniProt: | Q2T081 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTAVRKTLAR FAAVALAGWL AAAAGAAHAE VAAADPIDVA MRQCLARRDR SSTAGQIQCM DEARQQWQGE VDAAYQRLVK TAPADARRGW QESQRRWLAW RKDEAHLVRA VYETTQGTMY AMASADMRLQ PVRERALALR GAADRYAQPG GGKGAVHRVR PCMRDAACEH ALFDMNRYYE KLRARMPADS RQTLVAAQRE WAAFSDAMTP LVSEGERVDL IGARVATLKR FSETVNNR
NT Sequence
ATGACGGCAG TCCGCAAGAC GCTCGCGCGC TTTGCCGCCG TCGCGCTCGC CGGATGGCTC GCGGCGGCGG CGGGTGCGGC GCACGCCGAA GTCGCCGCCG CCGATCCGAT CGACGTCGCG ATGCGTCAGT GTCTCGCGCG GCGCGACCGG TCTTCGACGG CGGGCCAGAT CCAGTGCATG GACGAGGCGC GGCAGCAGTG GCAGGGCGAG GTCGACGCCG CGTACCAGCG GCTCGTGAAG ACCGCGCCCG CGGACGCGCG GCGCGGCTGG CAGGAAAGCC AGCGCCGCTG GCTCGCGTGG CGCAAGGACG AGGCGCATCT GGTGCGCGCG GTCTACGAGA CGACGCAAGG GACGATGTAC GCGATGGCGA GCGCCGACAT GCGGCTGCAG CCGGTGCGCG AGCGCGCGCT CGCGCTGCGC GGCGCCGCCG ACCGCTACGC GCAGCCGGGC GGCGGCAAGG GCGCCGTGCA CCGCGTGCGG CCCTGCATGC GCGATGCCGC GTGCGAGCAC GCGCTCTTCG ACATGAACCG CTATTACGAG AAATTGCGCG CGCGGATGCC CGCCGATTCG CGGCAGACGC TCGTCGCCGC GCAGCGCGAG TGGGCGGCGT TCAGCGACGC GATGACGCCG CTCGTCAGCG AGGGCGAGCG CGTCGATCTG ATCGGCGCGC GCGTCGCGAC GCTCAAGCGG TTCTCCGAGA CCGTCAACAA TCGC
Details for ButhA.17069.a.A2.GE34771
| HARVESTED ON: | 3/13/2012 |
| SEQUENCED ON: | 1/10/2017 |
| EXPECTED MW: | 25kDa |
| OBSERVED MW: | 26kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMADPIDVAM RQCLARRDRS STAGQIQCMD EARQQWQGEV DAAYQRLVKT APADARRGWQ ESQRRWLAWR KDEAHLVRAV YETTQGTMYA MASADMRLQP VRERALALRG AADRYAQPGG GKGAVHRVRP CMRDAACEHA LFDMNRYYEK LRARMPADSR QTLVAAQREW AAFSDAMTPL VSEGERVDLI GARVATLKRF SETVN
Validated NT Sequence
tttactttaa gaaggagana taccatggct catcaccatc accatcatat gggtaccctg gaagctcaga cccagggtcc tggttcgatg gccgatccga tcgacgtcgc gatgcgtcag tgtctcgcgc ggcgcgaccg gtcttcgacg gcgggccaga tccagtgcat ggacgaggcg cggcagcagt ggcagggcga ggtcgacgcc gcgtaccagc ggctcgtgaa gaccgcgccc gcggacgcgc ggcgcggctg gcaggaaagc cagcgccgct ggctcgcgtg gcgcaaggac gaggcgcatc tggtgcgcgc ggtctacgag acgacgcaag ggacgatgta cgcgatggcg agcgccgaca tgcggctgca gccggtgcgc gagcgcgcgc tcgcgctgcg cggcgccgcc gaccgctacg cgcagccggg cggcggcaag ggcgccgtgc accgcgtgcg gccctgcatg cgcgatgccg cgtgcgagca cgcgctcttc gacatgaacc gctattacga gaaattgcgc gcgcggatgc ccgccgattc gcggcagacg ctcgtcgccg cgcagcgcga gtgggcggcg ttcagcgacg cgatgacgcc gctcgtcagc gagggcgagc gcgtcgatct gatcggcgcg cgcgtcgcga cgctcaagcg gttctccgag accgtcaaca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMADPIDVAM RQCLARRDRS STAGQIQCMD EARQQWQGEV DAAYQRLVKT APADARRGWQ ESQRRWLAWR KDEAHLVRAV YETTQGTMYA MASADMRLQP VRERALALRG AADRYAQPGG GKGAVHRVRP CMRDAACEHA LFDMNRYYEK LRARMPADSR QTLVAAQREW AAFSDAMTPL VSEGERVDLI GARVATLKRF SETVNNR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccgatccg atcgacgtcg cgatgcgtca gtgtctcgcg cggcgcgacc ggtcttcgac ggcgggccag atccagtgca tggacgaggc gcggcagcag tggcagggcg aggtcgacgc cgcgtaccag cggctcgtga agaccgcgcc cgcggacgcg cggcgcggct ggcaggaaag ccagcgccgc tggctcgcgt ggcgcaagga cgaggcgcat ctggtgcgcg cggtctacga gacgacgcaa gggacgatgt acgcgatggc gagcgccgac atgcggctgc agccggtgcg cgagcgcgcg ctcgcgctgc gcggcgccgc cgaccgctac gcgcagccgg gcggcggcaa gggcgccgtg caccgcgtgc ggccctgcat gcgcgatgcc gcgtgcgagc acgcgctctt cgacatgaac cgctattacg agaaattgcg cgcgcggatg cccgccgatt cgcggcagac gctcgtcgcc gcgcagcgcg agtgggcggc gttcagcgac gcgatgacgc cgctcgtcag cgagggcgag cgcgtcgatc tgatcggcgc gcgcgtcgcg acgctcaagc ggttctccga gaccgtcaac aatcgctaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for ButhA.17069.a.A2.PS01486
| PURIFICATION DATe: | 5/2/2012 |
| CONCENTRATION: | 34.79mg/ml |
| OBSERVED MW: | 25kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMADPIDVAM RQCLARRDRS STAGQIQCMD EARQQWQGEV DAAYQRLVKT APADARRGWQ ESQRRWLAWR KDEAHLVRAV YETTQGTMYA MASADMRLQP VRERALALRG AADRYAQPGG GKGAVHRVRP CMRDAACEHA LFDMNRYYEK LRARMPADSR QTLVAAQREW AAFSDAMTPL VSEGERVDLI GARVATLKRF SETVN
Validated NT Sequence
tttactttaa gaaggagana taccatggct catcaccatc accatcatat gggtaccctg gaagctcaga cccagggtcc tggttcgatg gccgatccga tcgacgtcgc gatgcgtcag tgtctcgcgc ggcgcgaccg gtcttcgacg gcgggccaga tccagtgcat ggacgaggcg cggcagcagt ggcagggcga ggtcgacgcc gcgtaccagc ggctcgtgaa gaccgcgccc gcggacgcgc ggcgcggctg gcaggaaagc cagcgccgct ggctcgcgtg gcgcaaggac gaggcgcatc tggtgcgcgc ggtctacgag acgacgcaag ggacgatgta cgcgatggcg agcgccgaca tgcggctgca gccggtgcgc gagcgcgcgc tcgcgctgcg cggcgccgcc gaccgctacg cgcagccggg cggcggcaag ggcgccgtgc accgcgtgcg gccctgcatg cgcgatgccg cgtgcgagca cgcgctcttc gacatgaacc gctattacga gaaattgcgc gcgcggatgc ccgccgattc gcggcagacg ctcgtcgccg cgcagcgcga gtgggcggcg ttcagcgacg cgatgacgcc gctcgtcagc gagggcgagc gcgtcgatct gatcggcgcg cgcgtcgcga cgctcaagcg gttctccgag accgtcaaca
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMADPIDVAM RQCLARRDRS STAGQIQCMD EARQQWQGEV DAAYQRLVKT APADARRGWQ ESQRRWLAWR KDEAHLVRAV YETTQGTMYA MASADMRLQP VRERALALRG AADRYAQPGG GKGAVHRVRP CMRDAACEHA LFDMNRYYEK LRARMPADSR QTLVAAQREW AAFSDAMTPL VSEGERVDLI GARVATLKRF SETVNNR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCCGATCCG ATCGACGTCG CGATGCGTCA GTGTCTCGCG CGGCGCGACC GGTCTTCGAC GGCGGGCCAG ATCCAGTGCA TGGACGAGGC GCGGCAGCAG TGGCAGGGCG AGGTCGACGC CGCGTACCAG CGGCTCGTGA AGACCGCGCC CGCGGACGCG CGGCGCGGCT GGCAGGAAAG CCAGCGCCGC TGGCTCGCGT GGCGCAAGGA CGAGGCGCAT CTGGTGCGCG CGGTCTACGA GACGACGCAA GGGACGATGT ACGCGATGGC GAGCGCCGAC ATGCGGCTGC AGCCGGTGCG CGAGCGCGCG CTCGCGCTGC GCGGCGCCGC CGACCGCTAC GCGCAGCCGG GCGGCGGCAA GGGCGCCGTG CACCGCGTGC GGCCCTGCAT GCGCGATGCC GCGTGCGAGC ACGCGCTCTT CGACATGAAC CGCTATTACG AGAAATTGCG CGCGCGGATG CCCGCCGATT CGCGGCAGAC GCTCGTCGCC GCGCAGCGCG AGTGGGCGGC GTTCAGCGAC GCGATGACGC CGCTCGTCAG CGAGGGCGAG CGCGTCGATC TGATCGGCGC GCGCGTCGCG ACGCTCAAGC GGTTCTCCGA GACCGTCAAC AATCGCTAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA