SSGCID: Target Page

ButhA.16001.a

Metallo-beta-lactamase family protein

CENTER ID:	ButhA.16001.a
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.16001.a.B1.GE36219	Full length( ButhA.16001.a )		1	258

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.4716
RefSeq:	YP_442438.1
UniProt:	Q2SXB1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRFASLGSGS EGNALVVEAS SGATTTRVLL DCGFSAKELE RRLARVGLRV DDLDAILVTH EHGDHVGCAL TLARRASLPL YTSWGTARAV GADEAGVDLR VLWGDETAAV GDLCVLPYTV PHDAREPLQF VFSDGATRLG VLTDVGMSTP HIGTVLSGCD GLVLEANHDV AMLAASRYPA SLKARIGGSH GHLSNDAAAA ILAELDRSRL RHLVAAHLSQ QNNAPALAQA ALAAAWGGQA DDVLVASQDG GFGWLSLR

NT Sequence

GTGCGATTCG CGAGCCTCGG AAGCGGCAGC GAAGGCAATG CGCTCGTCGT CGAAGCGTCA AGTGGCGCGA CGACGACGCG CGTCCTGCTC GACTGCGGCT TCTCGGCGAA GGAGCTCGAA CGCCGGCTCG CACGTGTCGG TTTGCGCGTC GACGATCTCG ATGCGATCCT GGTCACTCAC GAACACGGCG ACCATGTCGG TTGCGCGCTG ACACTCGCCC GCCGGGCGTC GCTGCCGCTG TACACGAGCT GGGGCACCGC GCGCGCGGTC GGCGCGGACG AGGCCGGCGT CGATCTCCGC GTGCTCTGGG GCGACGAAAC AGCCGCGGTA GGCGATTTGT GCGTGCTTCC CTATACCGTT CCGCACGATG CGCGCGAACC GCTCCAGTTC GTCTTCAGCG ACGGCGCGAC GCGTCTTGGC GTGCTGACCG ACGTCGGCAT GTCTACGCCG CATATCGGCA CCGTGCTGAG CGGCTGCGAC GGCCTCGTGC TGGAGGCGAA TCACGACGTC GCGATGCTTG CGGCCAGCCG CTATCCGGCG TCGCTGAAGG CGCGCATCGG CGGCTCGCAC GGCCATCTGA GCAACGATGC GGCGGCCGCG ATCCTCGCCG AGCTCGATCG CAGCCGGCTG CGGCACTTGG TGGCCGCGCA CCTGAGCCAG CAGAACAACG CGCCCGCACT CGCGCAGGCG GCGCTTGCGG CGGCTTGGGG AGGGCAGGCG GACGACGTGC TCGTCGCGTC GCAGGACGGG GGATTCGGCT GGCTCAGCCT GCGC

Details for ButhA.16001.a.B1.GE36219

HARVESTED ON:	1/23/2013
SEQUENCED ON:	1/23/2013
EXPECTED MW:	28kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMV RFASLGSGSE GNALVVEASS GATTTRVLLD CGFSAKELER RLARVGLRVD DLDAILVTHE HGDHVGCALT LARRASLPLY TSWGTARAVG ADEAGVDLRV LWGDETAAVG DLCVLPYTVP HDAREPLQFV FSDGATRLGV LTDVGMSTPH IGTVLSGCDG LVLEANHDVA MLAASRYPAS LKARIGGSHG HLSNDAAAAI LAELDRSRLR HLVAAHLSQQ NNAPALAXAA LAAAWGGQAD DVLVASXDGG FGWLSLR

Validated NT Sequence

ttttnnnnnn nttaagaagg agatatacca tggctcacca ccaccaccac catatggtgc gattcgcgag cctcggaagc ggcagcgaag gcaatgcgct cgtcgtcgaa gcgtcaagtg gcgcgacgac gacgcgcgtc ctgctcgact gcggcttctc ggcgaaggag ctcgaacgcc ggctcgcacg tgtcggtttg cgcgtcgacg atctcgatgc gatcctggtc actcacgaac acggcgacca tgtcggttgc gcgctgacac tcgcccgccg ggcgtcgctg ccgctgtaca cgagctgggg caccgcgcgc gcggtcggcg cggacgaggc cggcgtcgat ctccgcgtgc tctggggcga cgaaacagcc gcggtaggcg atttgtgcgt gcttccctat accgttccgc acgatgcgcg cgaaccgctc cagttcgtct tcagcgacgg cgcgacgcgt cttggcgtgc tgaccgacgt cggcatgtct acgccgcata tcggcaccgt gctgagcggc tgcgacggcc tcgtgctgga ggcgaatcac gacgtcgcga tgcttgcggc cagccgctat ccggcgtcgc tgaaggcgcg catcggcggc tcgcacggcc atctgagcaa cgatgcggcg gccgcgatcc tcgccgagct cgatcgcagc cggctgcggc acttggtggc cgcgcacctg agccagcaga acaacgcgcc cgcactcgcg cangcggcgc ttgcggcggc ttggggaggg caggcggacg acgtgctcgt cgcgtcgcan gacgggggat tcggctggct cagcctgcgc taagtgagta aganagganc cnnnnnnnnn ncccccaaaa nnnnnnnnnn nnncgnnncn gcnnccnnnn tnccngncnc nnnncgnann gnnnnnnnnn nnnccnnnnn nnnncnnccn nnccccnnnn annncnnnnn nnnnnnc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtgc gattcgcgag cctcggaagc ggcagcgaag gcaatgcgct cgtcgtcgaa gcgtcaagtg gcgcgacgac gacgcgcgtc ctgctcgact gcggcttctc ggcgaaggag ctcgaacgcc ggctcgcacg tgtcggtttg cgcgtcgacg atctcgatgc gatcctggtc actcacgaac acggcgacca tgtcggttgc gcgctgacac tcgcccgccg ggcgtcgctg ccgctgtaca cgagctgggg caccgcgcgc gcggtcggcg cggacgaggc cggcgtcgat ctccgcgtgc tctggggcga cgaaacagcc gcggtaggcg atttgtgcgt gcttccctat accgttccgc acgatgcgcg cgaaccgctc cagttcgtct tcagcgacgg cgcgacgcgt cttggcgtgc tgaccgacgt cggcatgtct acgccgcata tcggcaccgt gctgagcggc tgcgacggcc tcgtgctgga ggcgaatcac gacgtcgcga tgcttgcggc cagccgctat ccggcgtcgc tgaaggcgcg catcggcggc tcgcacggcc atctgagcaa cgatgcggcg gccgcgatcc tcgccgagct cgatcgcagc cggctgcggc acttggtggc cgcgcacctg agccagcaga acaacgcgcc cgcactcgcg caggcggcgc ttgcggcggc ttggggaggg caggcggacg acgtgctcgt cgcgtcgcag gacgggggat tcggctggct cagcctgcgc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat