SSGCID: Target Page

ButhA.15999.a

Metallo-beta-lactamase family protein

CENTER ID:	ButhA.15999.a
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.15999.a.B1.GE36218	Full length( ButhA.15999.a )		1	214

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.6034
RefSeq:	YP_443645.1
UniProt:	Q2STV4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKVTLIPVTP FQQNCSLLVC EKTGRAAVVD PGGDFELIEQ EVARQNVRVE KVLLTHGHVD HCAGAKRLAA HYGVPIEGPH EDERFWIEKL PMQSERFGFP AAEAFEPDRW LDDGESVTFG EETLEVYHCP GHTPGHVVFF SRAHRLAIVG DVLFAGSIGR TDFPRGNHAD LIRSIREKLW PLGDDVTFVP GHGPVSTFGD ERRTNPFVAD GVAG

NT Sequence

ATGAAAGTCA CGCTCATTCC GGTCACGCCG TTCCAGCAGA ACTGCTCGCT GCTCGTCTGC GAAAAGACGG GGCGCGCCGC CGTCGTCGAC CCCGGCGGCG ATTTCGAACT GATCGAACAG GAAGTCGCCC GGCAGAACGT GCGCGTAGAG AAGGTGCTGC TCACGCACGG TCATGTCGAC CATTGCGCGG GCGCGAAGCG GCTCGCCGCG CATTACGGCG TGCCGATCGA GGGGCCGCAC GAAGACGAGC GCTTCTGGAT CGAGAAGTTG CCGATGCAAA GCGAACGTTT CGGCTTTCCG GCCGCCGAGG CGTTCGAGCC CGACCGCTGG CTCGACGACG GCGAATCGGT CACGTTCGGC GAGGAAACGC TCGAGGTCTA TCACTGCCCG GGGCACACGC CAGGCCACGT CGTGTTCTTC AGCCGCGCGC ATCGGCTCGC GATCGTCGGC GACGTACTGT TCGCCGGCTC GATCGGCCGC ACCGATTTCC CGCGCGGCAA CCACGCGGAC CTGATCCGCT CGATCCGCGA AAAGCTCTGG CCGCTCGGCG ACGACGTCAC GTTCGTGCCC GGCCACGGCC CGGTGTCGAC GTTCGGCGAC GAGCGCCGCA CGAATCCGTT CGTCGCAGAC GGGGTGGCGG GA

Details for ButhA.15999.a.B1.GE36218

HARVESTED ON:	1/23/2013
SEQUENCED ON:	1/23/2013
EXPECTED MW:	25kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMK VTLIPVTPFQ QNCSLLVCEK TGRAAVVDPG GDFELIEQEV ARQNVRVEKV LLTHGHVDHC AGAKRLAAHY GVPIEGPHED ERFWIEKLPM QSERFGFPAA EAFEPDRWLD DGESVTFGEE TLEVYHCPGH TPGHVVFFSR AHRLAIVGDV LFAGSIGRTD FPRGNHADLI RSIREKLWPL GDDVTFVPGH GPVSTFGDER RTNPFVADGV AG

Validated NT Sequence

atcttactca cttatcccgc caccccgtct gcgacgaacg gattcgtgcg gcgctcgtcg ccgaacgtcg acaccgggcc gtggccgggc acgaacgtga cgtcgtcgcc gagcggccag agcttttcgc ggatcgagcg gatcaggtcc gcgtggttgc cgcgcgggaa atcggtgcgg ccgatcgagc cggcgaacag tacgtcgccg acgatcgcga gccgatgcgc gcggctgaag aacacgacgt ggcctggcgt gtgccccggg cagtgataga cctcgagcgt ttcctcgccg aacgtgaccg attcgccgtc gtcgagccag cggtcgggct cgaacgcctc ggcggccgga aagccgaaac gttcgctttg catcggcaac ttctcgatcc agaagcgctc gtcttcgtgc ggcccctcga tcggcacgcc gtaatgcgcg gcgagccgct tcgcgcccgc gcaatggtcg acatgaccgt gcgtgagcag caccttctct acgcgcacgt tctgccgggc gacttcctgt tcgatcagtt cgaaatcgcc gccggggtcg acgacggcgg cgcgccccgt cttttcgcag acgagcagcg agcagttctg ctggaacggc gtgaccggaa tgagcgtgac tttcatatgg tggtggtggt ggtgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaag tcacgctcat tccggtcacg ccgttccagc agaactgctc gctgctcgtc tgcgaaaaga cggggcgcgc cgccgtcgtc gaccccggcg gcgatttcga actgatcgaa caggaagtcg cccggcagaa cgtgcgcgta gagaaggtgc tgctcacgca cggtcatgtc gaccattgcg cgggcgcgaa gcggctcgcc gcgcattacg gcgtgccgat cgaggggccg cacgaagacg agcgcttctg gatcgagaag ttgccgatgc aaagcgaacg tttcggcttt ccggccgccg aggcgttcga gcccgaccgc tggctcgacg acggcgaatc ggtcacgttc ggcgaggaaa cgctcgaggt ctatcactgc ccggggcaca cgccaggcca cgtcgtgttc ttcagccgcg cgcatcggct cgcgatcgtc ggcgacgtac tgttcgccgg ctcgatcggc cgcaccgatt tcccgcgcgg caaccacgcg gacctgatcc gctcgatccg cgaaaagctc tggccgctcg gcgacgacgt cacgttcgtg cccggccacg gcccggtgtc gacgttcggc gacgagcgcc gcacgaatcc gttcgtcgca gacggggtgg cgggatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat