SSGCID: Target Page

ButhA.15997.a

Metallo-beta-lactamase family protein

CENTER ID:	ButhA.15997.a
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.15997.a.B1.GE36217	Full length( ButhA.15997.a )		1	326

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.3012
RefSeq:	YP_440905.1
UniProt:	Q2T1P4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPLPFALDHI NLWLLRDEID GQAGWTVVDC GIASADIKAH WERIFDAHLD GLPVLRVIVT HCHPDHFGLA NWLCEGGERG RWNVRLWMTL GEYLFGCLMA AGNGSNAGGP AAADHFARHG LADPAALDKL RNRRSYFSDL VPAVPPRYRR LREGDALAIG AREWRVVTGY GHSPEHCALH CAADGVLISG DMVLPRISTN VSVFDLEPEA NPLALYLDSL GRYESMSEGT LVLPSHGKPF RGVRTRIAQL RAHHEARLAE VRAACRERPM SAADIVPIMF KRGGLDIHQM TFALGEAIAH LNLLWLAGEL ARETGADGVI RFRHAG

NT Sequence

ATGCCGCTGC CGTTCGCGCT CGACCACATC AATCTCTGGC TGCTGCGCGA CGAGATCGAC GGGCAGGCGG GCTGGACGGT CGTCGACTGC GGGATCGCGT CGGCGGACAT CAAGGCGCAT TGGGAGCGGA TCTTCGATGC GCATCTGGAC GGCCTGCCCG TGCTGCGCGT GATCGTCACG CACTGCCATC CGGATCATTT CGGACTCGCG AACTGGCTGT GCGAAGGCGG CGAGCGCGGG CGCTGGAACG TGCGGCTGTG GATGACGCTC GGCGAATACC TGTTCGGCTG CCTGATGGCG GCCGGAAACG GTTCGAACGC GGGCGGGCCC GCCGCCGCCG ATCATTTCGC GCGCCATGGC CTGGCCGATC CGGCCGCGCT CGACAAGCTG CGCAACCGCC GCAGCTACTT CTCGGATCTC GTGCCCGCCG TGCCGCCGCG CTACCGGCGG CTGCGCGAAG GCGACGCGCT CGCGATCGGC GCGCGCGAAT GGCGCGTCGT GACGGGCTAT GGCCATTCGC CCGAACATTG CGCGCTCCAC TGCGCGGCCG ACGGCGTGCT GATCTCCGGC GACATGGTGC TGCCGCGAAT CTCGACGAAC GTGTCGGTGT TCGACCTCGA GCCGGAGGCG AACCCGCTCG CGCTGTATCT GGATTCGCTC GGCCGCTACG AATCGATGTC GGAGGGCACG CTCGTGCTGC CTTCGCACGG CAAGCCGTTT CGCGGCGTGA GAACGCGCAT CGCGCAACTG CGCGCGCATC ACGAAGCGCG CCTCGCCGAA GTGCGCGCCG CGTGCCGCGA GCGGCCGATG AGCGCGGCCG ACATCGTGCC GATCATGTTC AAGCGCGGCG GGCTCGACAT TCATCAGATG ACTTTCGCGC TCGGCGAGGC GATCGCGCAC CTGAACCTGC TGTGGCTCGC GGGCGAACTC GCGCGCGAGA CGGGTGCGGA CGGCGTGATC CGGTTCCGGC ACGCGGGC

Details for ButhA.15997.a.B1.GE36217

HARVESTED ON:	1/23/2013
SEQUENCED ON:	1/23/2013
EXPECTED MW:	37kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	96
PERCENT COVERAGE:	101

Validated AA Sequence

MAHHHHHHMP LPFALDHINL WLLRDEIDGQ AGWTVVDCGI ASADIKAHWE RIFDAHLDGL PVLRVIVTHC HPDHFGLANW LCEGGERGRW NVRLWMTLGE YLFGCLMAAG NGSNAGGPAA ADHFARHGLA DPAALDKLRN RRSYFSDLVP AVPPRYRRLR EGDALAIGAR EWRVVTGYGH SPEHCALHCA ADGVLISGDM VLPRISTNVS VFDLEPEANP LALYLDSLGR YESMSEGTLV LPSHGKPFRG VRTRIAQLRA HHEARLAEVR AACRERPMSA ADIVPIMFKR GGLDIHXMTF ALGEAIAHLN LLWXAGELXR ETGXDGXIXX XXXXLXXX

Validated NT Sequence

ttttnnttac tttaagaagg aganatacca tggctcacca ccaccaccac catatgccgc tgccgttcgc gctcgaccac atcaatctct ggctgctgcg cgacgagatc gacgggcagg cgggctggac ggtcgtcgac tgcgggatcg cgtcggcgga catcaaggcg cattgggagc ggatcttcga tgcgcatctg gacggcctgc ccgtgctgcg cgtgatcgtc acgcactgcc atccggatca tttcggactc gcgaactggc tgtgcgaagg cggcgagcgc gggcgctgga acgtgcggct gtggatgacg ctcggcgaat acctgttcgg ctgcctgatg gcggccggaa acggttcgaa cgcgggcggg cccgccgccg ccgatcattt cgcgcgccat ggcctggccg atccggccgc gctcgacaag ctgcgcaacc gccgcagcta cttctcggat ctcgtgcccg ccgtgccgcc gcgctaccgg cggctgcgcg aaggcgacgc gctcgcgatc ggcgcgcgcg aatggcgcgt cgtgacgggc tatggccatt cgcccgaaca ttgcgcgctc cactgcgcgg ccgacggcgt gctgatctcc ggcgacatgg tgctgccgcg aatctcgacg aacgtgtcgg tgttcgacct cgagccggag gcgaacccgc tcgcgctgta tctggattcg ctcggccgct acgaatcgat gtcggagggc acgctcgtgc tgccttcgca cggcaagccg tttcgcggcg tgagaacgcg catcgcgcaa ctgcgcgcgc atcacgaagc gcgcctcgcc gaagtgcgcg ccgcgtgccg cgagcggccg atgagcgcgg ccgacatcgt gccgatcatg ttcaagcgcg gcgggctcga cattcatcan atgactttcg cgctcggcga ggcgatcgcg cacctgaacc tgctgtggnt cgcgggcgaa ctcncgcgcg agacgggtgn ngacggnntg atccngnttc nngnncnnng gctaantgnn naana

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccgc tgccgttcgc gctcgaccac atcaatctct ggctgctgcg cgacgagatc gacgggcagg cgggctggac ggtcgtcgac tgcgggatcg cgtcggcgga catcaaggcg cattgggagc ggatcttcga tgcgcatctg gacggcctgc ccgtgctgcg cgtgatcgtc acgcactgcc atccggatca tttcggactc gcgaactggc tgtgcgaagg cggcgagcgc gggcgctgga acgtgcggct gtggatgacg ctcggcgaat acctgttcgg ctgcctgatg gcggccggaa acggttcgaa cgcgggcggg cccgccgccg ccgatcattt cgcgcgccat ggcctggccg atccggccgc gctcgacaag ctgcgcaacc gccgcagcta cttctcggat ctcgtgcccg ccgtgccgcc gcgctaccgg cggctgcgcg aaggcgacgc gctcgcgatc ggcgcgcgcg aatggcgcgt cgtgacgggc tatggccatt cgcccgaaca ttgcgcgctc cactgcgcgg ccgacggcgt gctgatctcc ggcgacatgg tgctgccgcg aatctcgacg aacgtgtcgg tgttcgacct cgagccggag gcgaacccgc tcgcgctgta tctggattcg ctcggccgct acgaatcgat gtcggagggc acgctcgtgc tgccttcgca cggcaagccg tttcgcggcg tgagaacgcg catcgcgcaa ctgcgcgcgc atcacgaagc gcgcctcgcc gaagtgcgcg ccgcgtgccg cgagcggccg atgagcgcgg ccgacatcgt gccgatcatg ttcaagcgcg gcgggctcga cattcatcag atgactttcg cgctcggcga ggcgatcgcg cacctgaacc tgctgtggct cgcgggcgaa ctcgcgcgcg agacgggtgc ggacggcgtg atccggttcc ggcacgcggg ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat