ButhA.12800.y

Transcriptional regulator, LysR family

CENTER ID: ButhA.12800.y
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.12800.y.A1.GE34786 Full length( ButhA.12800.y ) 1 314
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.113
RefSeq: YP_438306.1
UniProt: Q2T943

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNHLFSAMRV FLKVAETHHF SHAANQLNLS PSLVTRYVGD LESHLGVKLL QRSTRKTVLT EVGVRYAEGC RALLANLHEI ESRATHESSA IAGDLHVVAL DSITSPELAG LFAEYQSRHP DVSLRITQTD SEVDLLGGGF DVGIVADTMI TSVSLVSRTL IHAPLVAVAS PGYLLQAPPP ARPADLARHR IIGLAAGART YRWTGKNNAV DTTQIDTPIT VNNAPMQRQL VLAGGGIGLL PEYLVANDLR IGTLTRLLGD YRIANDGVTI SLVYPGREFL PGKVRAFVDL AAARFKLPMI SVPRTVGRVD MSVA
NT Sequence
GTGAACCACC TATTCAGTGC GATGCGAGTC TTTCTCAAGG TTGCCGAAAC ACATCACTTC TCGCATGCGG CGAACCAGTT GAACCTCTCG CCGTCGCTGG TGACGCGCTA TGTCGGCGAT CTCGAATCGC ACCTCGGCGT CAAGCTGCTG CAACGCTCGA CGCGCAAGAC CGTCCTGACC GAAGTCGGCG TGCGTTATGC GGAGGGCTGC CGCGCGCTGC TCGCGAATCT CCACGAGATC GAATCGCGCG CGACGCACGA ATCGAGCGCG ATCGCGGGCG ATCTGCATGT CGTCGCACTC GACAGCATCA CGTCGCCGGA GCTGGCCGGA CTGTTCGCCG AATACCAGTC ACGCCACCCC GACGTATCGC TGCGCATCAC GCAAACCGAT TCGGAAGTCG ATCTGCTCGG CGGCGGATTC GATGTCGGCA TCGTAGCGGA TACGATGATC ACGTCGGTAT CGCTCGTGTC GCGCACGCTG ATTCACGCGC CGCTTGTCGC CGTCGCGTCG CCCGGCTATC TGCTGCAAGC GCCGCCGCCC GCGCGGCCGG CGGACCTCGC CCGCCACCGG ATCATCGGGC TCGCCGCCGG CGCGCGGACT TATCGGTGGA CGGGCAAGAA CAACGCGGTC GACACCACTC AGATCGACAC GCCGATCACC GTCAATAACG CGCCGATGCA GCGGCAACTC GTGCTGGCGG GCGGCGGCAT CGGGCTGCTG CCCGAGTACC TCGTCGCGAA CGATCTGCGC ATCGGCACGC TGACGCGGCT GCTCGGCGAC TATCGAATCG CCAACGACGG CGTCACGATA TCGCTCGTGT ACCCGGGCCG GGAATTCCTG CCCGGCAAGG TTCGCGCCTT CGTCGATCTG GCCGCCGCCC GCTTCAAACT GCCGATGATT TCGGTGCCGC GCACCGTCGG CAGGGTGGAT ATGAGCGTCG CG
Details for ButhA.12800.y.A1.GE34786
HARVESTED ON: 3/13/2012
SEQUENCED ON: 3/12/2012
EXPECTED MW: 36kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 87
PERCENT COVERAGE: 72
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVNHLFSAM RVFLKVAETH HFSHAANQLN LSPSLVTRYV GDLESHLGVK LLQRSTRKTV LTEVGVRYAE GCRALLANLH EIESRATHES SAIAGDLHVV ALDSITSPEL AGLFAEYQSR HPDVSLRITQ TDSEVDLLGG GFDVGIVADT MITSVSLVSR TLIHAPLVAV ASPGYLLQAP PPRAAGGPRP PPDHRARRRR ADLSVDGQEQ RGRHHSDRHA DHRQ
Validated NT Sequence
ttttnnttan ctttaagaag gaganatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga accacctatt cagtgcgatg cgagtctttc tcaaggttgc cgaaacacat cacttctcgc atgcggcgaa ccagttgaac ctctcgccgt cgctggtgac gcgctatgtc ggcgatctcg aatcgcacct cggcgtcaag ctgctgcaac gctcgacgcg caagaccgtc ctgaccgaag tcggcgtgcg ttatgcggag ggctgccgcg cgctgctcgc gaatctccac gagatcgaat cgcgcgcgac gcacgaatcg agcgcgatcg cgggcgatct gcatgtcgtc gcactcgaca gcatcacgtc gccggagctg gccggactgt tcgccgaata ccagtcacgc caccccgacg tatcgctgcg catcacgcaa accgattcgg aagtcgatct gctcggcggc ggattcgatg tcggcatcgt agcggatacg atgatcacgt cggtatcgct cgtgtcgcgc acgctgattc acgcgccgct tgtcgccgtc gcgtcgcccg gctatctgct gcaagcgccg ccgccccgcg cggccggcgg acctcgcccg ccaccggatc atcgggctcg ccgccggcgc gcggacttat cggtggacgg gcaagaacaa cgcggtcgac accactcaga tcgacacgcc gatcaccgtc aataacgcgc cgatgcagcg ncnnnngnnn nnngnnggnn ggnggcatcg ggctgctgcc cgagtacctc gtcgcgaacg atctgcgcat cnncacgctg acgcggctgc tcggcgacta tcgaatcgcc aacgacgnnn tcncgatatc gctcgtgnac ccggggccgg gaattcctgn ccggnaaggt tcgnnncttc ntcnnnnnng nccnncnnnc nnntcaaact gncgatgaat ttnngnngcc nnnnnncnnn cngnngggan annnancnnn nnnnnnanna anannnnnna nnnnttnngn nnncnagctt nnna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVNHLFSAM RVFLKVAETH HFSHAANQLN LSPSLVTRYV GDLESHLGVK LLQRSTRKTV LTEVGVRYAE GCRALLANLH EIESRATHES SAIAGDLHVV ALDSITSPEL AGLFAEYQSR HPDVSLRITQ TDSEVDLLGG GFDVGIVADT MITSVSLVSR TLIHAPLVAV ASPGYLLQAP PPARPADLAR HRIIGLAAGA RTYRWTGKNN AVDTTQIDTP ITVNNAPMQR QLVLAGGGIG LLPEYLVAND LRIGTLTRLL GDYRIANDGV TISLVYPGRE FLPGKVRAFV DLAAARFKLP MISVPRTVGR VDMSVA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaaccac ctattcagtg cgatgcgagt ctttctcaag gttgccgaaa cacatcactt ctcgcatgcg gcgaaccagt tgaacctctc gccgtcgctg gtgacgcgct atgtcggcga tctcgaatcg cacctcggcg tcaagctgct gcaacgctcg acgcgcaaga ccgtcctgac cgaagtcggc gtgcgttatg cggagggctg ccgcgcgctg ctcgcgaatc tccacgagat cgaatcgcgc gcgacgcacg aatcgagcgc gatcgcgggc gatctgcatg tcgtcgcact cgacagcatc acgtcgccgg agctggccgg actgttcgcc gaataccagt cacgccaccc cgacgtatcg ctgcgcatca cgcaaaccga ttcggaagtc gatctgctcg gcggcggatt cgatgtcggc atcgtagcgg atacgatgat cacgtcggta tcgctcgtgt cgcgcacgct gattcacgcg ccgcttgtcg ccgtcgcgtc gcccggctat ctgctgcaag cgccgccgcc cgcgcggccg gcggacctcg cccgccaccg gatcatcggg ctcgccgccg gcgcgcggac ttatcggtgg acgggcaaga acaacgcggt cgacaccact cagatcgaca cgccgatcac cgtcaataac gcgccgatgc agcggcaact cgtgctggcg ggcggcggca tcgggctgct gcccgagtac ctcgtcgcga acgatctgcg catcggcacg ctgacgcggc tgctcggcga ctatcgaatc gccaacgacg gcgtcacgat atcgctcgtg tacccgggcc gggaattcct gcccggcaag gttcgcgcct tcgtcgatct ggccgccgcc cgcttcaaac tgccgatgat ttcggtgccg cgcaccgtcg gcagggtgga tatgagcgtc gcgtaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa