SSGCID: Target Page

ButhA.12800.t

Transcriptional regulator, LysR family

CENTER ID:	ButhA.12800.t
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.12800.t.A1.GE34778	Full length( ButhA.12800.t )		1	299

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.1698
RefSeq:	YP_439738.1
UniProt:	Q2T511

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDRITAMQVF VETAERGSVS AAAQHLDMSR AMASRYVAFI EQWTGARLLH RTTRRLTLTA AGAQMLPLCR DMLGLADHVA TVVAKPDDAP RGALRITASA IFAQTHVTDA VLDYLARYPA VSVDLLVTDR TADLVDERID LAIRITNEVD PSLIARRLGT CRSVLCASPG YLAEHGAPRQ PHDLARHNCL TYAYFGQSLW HLTRDGEPAT VPVQGNLSAN DALVLLRAAI AGGGIALLPT FAAAEPIRAG TLARVLPDCA APDLGIYAVY ASRKQMPLAM RAMIDFLAER FGDTPAWDA

NT Sequence

ATGGATCGAA TCACCGCGAT GCAGGTGTTC GTGGAGACGG CCGAGCGCGG CAGCGTGTCG GCCGCCGCGC AGCATCTGGA CATGTCGCGC GCGATGGCGT CGCGCTACGT TGCATTCATC GAGCAATGGA CGGGGGCGCG GCTGTTGCAC CGGACCACGC GGCGCCTGAC GCTGACCGCG GCGGGCGCGC AGATGCTGCC GCTCTGCCGC GACATGCTCG GGCTCGCCGA TCACGTCGCG ACCGTAGTCG CGAAGCCGGA CGACGCGCCG CGCGGCGCGC TGAGAATCAC CGCGAGCGCG ATCTTCGCGC AGACGCACGT GACCGACGCG GTGCTCGACT ACCTGGCCCG CTACCCGGCC GTGTCGGTCG ATTTGCTCGT CACCGACCGC ACCGCCGATC TCGTCGACGA ACGAATCGAC CTGGCCATCC GGATCACGAA CGAGGTCGAC CCAAGCCTGA TCGCGCGGCG GCTCGGCACG TGCCGCTCGG TGCTGTGCGC ATCGCCCGGC TATCTGGCCG AGCACGGCGC GCCGCGGCAG CCGCACGATC TCGCGCGGCA CAACTGCCTG ACGTATGCCT ATTTCGGGCA GAGCCTGTGG CACCTGACGC GCGACGGCGA GCCGGCGACG GTGCCCGTGC AGGGCAATCT GAGCGCGAAC GACGCACTCG TGCTGCTGCG TGCGGCGATC GCGGGCGGCG GCATCGCGCT GCTGCCGACC TTCGCGGCGG CCGAGCCGAT CCGCGCGGGC ACGCTCGCGC GCGTGCTGCC CGACTGCGCG GCGCCCGATC TCGGCATCTA TGCGGTCTAC GCATCGCGCA AGCAGATGCC GCTCGCGATG CGCGCGATGA TCGATTTCCT GGCCGAGCGT TTCGGCGACA CCCCCGCATG GGACGCG

Details for ButhA.12800.t.A1.GE34778

HARVESTED ON:	3/13/2012
SEQUENCED ON:	3/12/2012
EXPECTED MW:	34kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	94
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMDRITAMQV FVETAERGSV SAAAQHLDMS RAMASRYVAF IEQWTGARLL HRTTRRLTLT AAGAQMLPLC RDMLGLADHV ATVVAKPDDA PRGALRITAS AIFAQTHVTD AVLDYLARYP AVSVDLLVTD RTADLVDERI DLAIRITNEV DPSLIARRLG TCRSVLCASP GYLAEHGAPR QPHDLARHNC LTYAYFGQSL WHLTRDGEPA TVPVQGNLSA NDALVLLRAA IAGGGIALLP TFAAAEPIRA GTLARVLPDX AXPDLGIYAV YASRKXXPXA MXAMIDXLXX XSXXPXMGX

Validated NT Sequence

annnattttn nttactttaa gaaggagana taccatggct catcaccatc accatcatat gggtaccctg gaagctcaga cccagggtcc tggttcgatg gatcgaatca ccgcgatgca ggtgttcgtg gagacggccg agcgcggcag cgtgtcggcc gccgcgcagc atctggacat gtcgcgcgcg atggcgtcgc gctacgttgc attcatcgag caatggacgg gggcgcggct gttgcaccgg accacgcggc gcctgacgct gaccgcggcg ggcgcgcaga tgctgccgct ctgccgcgac atgctcgggc tcgccgatca cgtcgcgacc gtagtcgcga agccggacga cgcgccgcgc ggcgcgctga gaatcaccgc gagcgcgatc ttcgcgcaga cgcacgtgac cgacgcggtg ctcgactacc tggcccgcta cccggccgtg tcggtcgatt tgctcgtcac cgaccgcacc gccgatctcg tcgacgaacg aatcgacctg gccatccgga tcacgaacga ggtcgaccca agcctgatcg cgcggcggct cggcacgtgc cgctcggtgc tgtgcgcatc gcccggctat ctggccgagc acggcgcgcc gcggcagccg cacgatctcg cgcggcacaa ctgcctgacg tatgcctatt tcgggcagag cctgtggcac ctgacgcgcg acggcgagcc ggcgacggtg cccgtgcagg gcaatctgag cgcgaacgac gcactcgtgc tgctgcgtgc ggcgatcgcg ggcggcggca tcgcgctgct gccgaccttc gcggcggccg agccgatccg cgcgggcacg ctcgcgcgcg tgctgcccga ctgngcggnn cccgatctcg gcatctatgc ggtctacgca tcgcgcaagc ngangccnnt cgcgatgcnc gcgatgatcg atttnctgnn nancgnttcg nnnanncccc ncatgggnnn gtaataaana gcncgaanna gtnctgcanc nagcnnnnnn nnnnncnnnn nnnnnnncnn nnnnnnnnna

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggatcgaatc accgcgatgc aggtgttcgt ggagacggcc gagcgcggca gcgtgtcggc cgccgcgcag catctggaca tgtcgcgcgc gatggcgtcg cgctacgttg cattcatcga gcaatggacg ggggcgcggc tgttgcaccg gaccacgcgg cgcctgacgc tgaccgcggc gggcgcgcag atgctgccgc tctgccgcga catgctcggg ctcgccgatc acgtcgcgac cgtagtcgcg aagccggacg acgcgccgcg cggcgcgctg agaatcaccg cgagcgcgat cttcgcgcag acgcacgtga ccgacgcggt gctcgactac ctggcccgct acccggccgt gtcggtcgat ttgctcgtca ccgaccgcac cgccgatctc gtcgacgaac gaatcgacct ggccatccgg atcacgaacg aggtcgaccc aagcctgatc gcgcggcggc tcggcacgtg ccgctcggtg ctgtgcgcat cgcccggcta tctggccgag cacggcgcgc cgcggcagcc gcacgatctc gcgcggcaca actgcctgac gtatgcctat ttcgggcaga gcctgtggca cctgacgcgc gacggcgagc cggcgacggt gcccgtgcag ggcaatctga gcgcgaacga cgcactcgtg ctgctgcgtg cggcgatcgc gggcggcggc atcgcgctgc tgccgacctt cgcggcggcc gagccgatcc gcgcgggcac gctcgcgcgc gtgctgcccg actgcgcggc gcccgatctc ggcatctatg cggtctacgc atcgcgcaag cagatgccgc tcgcgatgcg cgcgatgatc gatttcctgg ccgagcgttt cggcgacacc cccgcatggg acgcgtaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa