SSGCID: Target Page

ButhA.12800.s

Transcriptional regulator, LysR family

CENTER ID:	ButhA.12800.s
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.12800.s.A1.GE32067	Full length( ButhA.12800.s )		1	354

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.1821
RefSeq:	YP_439840.1
UniProt:	Q2T4Q9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MCGPCWRTIS SLHTFRTGMD TLQNMRVFAR VVEAGSFTAA AQSLNSTTGA MSRAVSELEA RLRTRLMNRS TRRLALTPAG ERYLLRCRQI LADVDQAEEE ASCAHERPAG VLRMHSFASI GQHYVLPALT AYRSQHPDVS IELTLSQRMP DLFDGSSDMA VVTASSLPDS ELVSHLLGST FSILCATPAY LRAHGAPRTP RDLARHACLT LRTPAFPTNE WLLEGPNGAE EIKVDGPVQT NTAESLAVAI RAGIGIGMLP LYAAIEDLRC GNLVRVLPAH RLQKMNVYAL YPSRRFIDAK VRTWVEALRF HMPKMIARDE AMLSAIVAQA SDATQSAIAG ALDVGLESAH ALSH

NT Sequence

ATGTGCGGGC CCTGCTGGAG GACAATATCG TCTCTACATA CGTTTCGGAC GGGAATGGAT ACGCTACAGA ACATGCGGGT GTTCGCCCGC GTGGTGGAGG CGGGCAGCTT CACGGCGGCC GCGCAATCGC TGAATTCGAC GACGGGCGCG ATGTCGCGCG CTGTGTCGGA GCTCGAGGCG CGGCTGCGCA CGCGCCTGAT GAACCGCTCG ACGCGGCGCC TCGCGTTGAC GCCCGCGGGC GAGCGCTATC TGCTGCGCTG CCGGCAGATT CTCGCCGACG TCGATCAGGC CGAAGAAGAG GCGAGCTGCG CGCACGAGCG CCCGGCAGGC GTGCTGCGGA TGCACAGCTT CGCGAGCATC GGCCAGCACT ATGTGCTGCC CGCGCTCACC GCGTACCGCA GCCAGCATCC GGACGTATCG ATCGAGCTCA CGCTGTCGCA GCGGATGCCG GACCTGTTCG ACGGATCGAG CGACATGGCG GTCGTGACCG CATCGTCGCT GCCCGATTCC GAGCTGGTGT CGCATCTGCT CGGCTCGACG TTCAGCATTC TGTGCGCGAC GCCCGCGTAT CTGCGCGCGC ACGGCGCGCC GCGCACGCCG CGCGATCTCG CGCGGCACGC GTGCCTCACG CTGCGCACGC CGGCGTTCCC GACGAACGAA TGGCTGCTCG AAGGGCCGAA CGGCGCCGAG GAGATCAAGG TCGACGGCCC GGTGCAGACC AATACCGCCG AGTCGCTCGC GGTCGCGATC CGCGCGGGCA TCGGCATCGG CATGCTGCCG CTTTACGCGG CGATCGAGGA CCTGCGCTGC GGCAACCTCG TGCGCGTGCT GCCCGCGCAC CGGCTGCAGA AGATGAACGT CTACGCGCTG TATCCGTCGC GCCGCTTCAT CGATGCGAAA GTGCGCACCT GGGTCGAAGC GCTGCGCTTT CACATGCCGA AGATGATCGC GCGCGACGAG GCGATGCTGT CGGCGATCGT CGCGCAAGCG TCCGACGCGA CGCAAAGCGC GATCGCCGGC GCGCTCGACG TCGGACTCGA ATCGGCGCAC GCGCTCAGCC AC

Details for ButhA.12800.s.A1.GE32067

HARVESTED ON:	4/19/2011
SEQUENCED ON:	4/25/2011
EXPECTED MW:	40kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	94

Validated AA Sequence

Validated NT Sequence

agtggctgag cgcgtgcgcc gattcgagtc cgacgtcgag cgcgccggcg atcgcgcttt gcgtcgcgtc ggacgcttgc gcgacgatcg ccgacagcat cgcctcgtcg cgcgcgatca tcttcggcat gtgaaagcgc agcgcttcga cccaggtgcg cactttcgca tcgatgaagc ggcgcgacgg atacagcgcg tagacgttca tcttctgcag ccggtgcgcg ggcagcacgc gcacgaggtt gccgcagcgc aggtcctcga tcgccgcgta aagcggcagc atgccgatgc cgatgcccgc gcggatcgcg accgcgagcg actcggcggt attggtctgc accgggccgt cgaccttgat ctcctcggcg ccgttcggcc cttcgagcag ccattcgttc gtcgggaacg ccggcgtgcg cagcgtgagg cacgcgtgcc gcgcgagatc gcgcggcgtg cgcggcgcgc cgtgcgcgcg cagatacgcg ggcgtcgcgc acagaatgct gaacgtcgag ccgagcagat gcgacaccag ctcggaatcg ggcagcgacg atgcggtcac gaccgccatg tcgctcgatc cgtcgaacag gtccggcatc cgctgcgaca gcgtgagctc gatcgatacg tccggatgct ggctgcggta cgcggtgagc gcgggcagca catagtgctg gccgatgctc gcgaagctgt gcatccgcag cacgcctgcc gggcgctcgt gcgcgcagct cgcctcttct tcggcctgat cgacgtcggc gagaatctgc cggcagcgca gcagatagcg ctcgcccgcg ggcgtcaacg cgaggcgccg cgtcgagcgg ttcatcaggc gcgtgcgcag ccgcgcctcg agctccgaca cagcgcgcga catcgcgccc gtcgtcgaat tcagcgattg cgcggccgcc gtgaagctgc ccgcctccac cacgcgggcg aacacccgca tgttctgtag cgtatccatt cccgtccgaa acgtatgtag agacgatatt gtcctccagc agggcccgca catcgaacca ggaccctggg tctgagcttc cagggta

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMCGPCWRTI SSLHTFRTGM DTLQNMRVFA RVVEAGSFTA AAQSLNSTTG AMSRAVSELE ARLRTRLMNR STRRLALTPA GERYLLRCRQ ILADVDQAEE EASCAHERPA GVLRMHSFAS IGQHYVLPAL TAYRSQHPDV SIELTLSQRM PDLFDGSSDM AVVTASSLPD SELVSHLLGS TFSILCATPA YLRAHGAPRT PRDLARHACL TLRTPAFPTN EWLLEGPNGA EEIKVDGPVQ TNTAESLAVA IRAGIGIGML PLYAAIEDLR CGNLVRVLPA HRLQKMNVYA LYPSRRFIDA KVRTWVEALR FHMPKMIARD EAMLSAIVAQ ASDATQSAIA GALDVGLESA HALSH

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtgcgggccc tgctggagga caatatcgtc tctacatacg tttcggacgg gaatggatac gctacagaac atgcgggtgt tcgcccgcgt ggtggaggcg ggcagcttca cggcggccgc gcaatcgctg aattcgacga cgggcgcgat gtcgcgcgct gtgtcggagc tcgaggcgcg gctgcgcacg cgcctgatga accgctcgac gcggcgcctc gcgttgacgc ccgcgggcga gcgctatctg ctgcgctgcc ggcagattct cgccgacgtc gatcaggccg aagaagaggc gagctgcgcg cacgagcgcc cggcaggcgt gctgcggatg cacagcttcg cgagcatcgg ccagcactat gtgctgcccg cgctcaccgc gtaccgcagc cagcatccgg acgtatcgat cgagctcacg ctgtcgcagc ggatgccgga cctgttcgac ggatcgagcg acatggcggt cgtgaccgca tcgtcgctgc ccgattccga gctggtgtcg catctgctcg gctcgacgtt cagcattctg tgcgcgacgc ccgcgtatct gcgcgcgcac ggcgcgccgc gcacgccgcg cgatctcgcg cggcacgcgt gcctcacgct gcgcacgccg gcgttcccga cgaacgaatg gctgctcgaa gggccgaacg gcgccgagga gatcaaggtc gacggcccgg tgcagaccaa taccgccgag tcgctcgcgg tcgcgatccg cgcgggcatc ggcatcggca tgctgccgct ttacgcggcg atcgaggacc tgcgctgcgg caacctcgtg cgcgtgctgc ccgcgcaccg gctgcagaag atgaacgtct acgcgctgta tccgtcgcgc cgcttcatcg atgcgaaagt gcgcacctgg gtcgaagcgc tgcgctttca catgccgaag atgatcgcgc gcgacgaggc gatgctgtcg gcgatcgtcg cgcaagcgtc cgacgcgacg caaagcgcga tcgccggcgc gctcgacgtc ggactcgaat cggcgcacgc gctcagccac taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa