ButhA.12800.r

Transcriptional regulator, LysR family

CENTER ID: ButhA.12800.r
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.12800.r.A1.GE34789 Full length( ButhA.12800.r ) 1 321
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.2242
RefSeq: YP_440220.1
UniProt: Q2T3M9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTIDAHNLND LMYFSQVVEH GGFSAAERVL GISKSRLSRR LTELEATLGV RLLQRSTRKL ALTEAGQLFY QHCQAMLSEA QAAMNAVQQL RSAPRGTVRV SVPVTLSQTM LSRLLPEFLR RFPEVRVQIR VTNRVIDLFE DSIDVALRVR SEPPQNANIV VRPLWRTEQM LVGAPSLLQQ NAPPLVPDDL SRFDTLDTPS GDGRHVFNLI APDGTRHAHE HEPRLVTADL MTIREAVLDG VGIAALPESM YGNALRAGQL SPVMPGWTLP VPQLYAVFVS RQGMPPAVRA FVDYLVEKLD SADYKESSCP ERGAKDAKIS I
NT Sequence
ATGACTATCG ATGCACATAA CCTGAACGAC TTGATGTACT TCTCTCAGGT AGTCGAGCAT GGCGGCTTCT CGGCCGCGGA ACGCGTGCTC GGCATCTCGA AGTCGCGCCT GTCGCGACGG CTGACCGAGC TCGAGGCGAC GCTCGGTGTG CGCCTGTTGC AGCGCTCGAC CCGCAAGCTC GCGTTGACCG AGGCGGGCCA GCTTTTCTAT CAGCATTGCC AAGCAATGTT GTCGGAGGCG CAGGCCGCGA TGAACGCGGT GCAGCAACTG CGCTCGGCGC CGCGCGGCAC GGTGCGCGTG AGCGTGCCCG TCACGCTGTC GCAGACGATG CTGTCGCGCC TGCTGCCGGA ATTCCTGCGC CGCTTTCCGG AAGTGCGCGT GCAGATTCGC GTGACGAATC GCGTGATCGA CCTGTTTGAA GATTCGATCG ACGTCGCGCT GCGCGTGCGA TCCGAGCCGC CGCAGAATGC GAACATCGTC GTGCGGCCGC TGTGGCGTAC CGAGCAGATG CTCGTTGGCG CGCCGAGCCT GCTGCAGCAG AACGCGCCGC CTCTCGTGCC CGACGATCTG TCGAGATTCG ATACGCTCGA TACGCCGTCA GGCGACGGGC GGCACGTATT CAACCTGATC GCCCCCGACG GCACGCGCCA TGCGCACGAG CACGAGCCGC GGCTCGTGAC CGCCGATCTG ATGACGATTC GCGAAGCCGT GCTCGACGGG GTCGGAATCG CCGCGCTGCC GGAATCGATG TACGGCAACG CATTGCGTGC GGGGCAATTG TCGCCGGTGA TGCCGGGATG GACGCTGCCC GTGCCGCAGC TCTATGCGGT GTTCGTGTCG CGGCAGGGGA TGCCGCCTGC CGTGCGCGCG TTCGTCGATT ACCTCGTCGA GAAGCTCGAC AGCGCGGACT ACAAGGAGTC GAGTTGCCCG GAGCGCGGCG CGAAGGACGC GAAGATTTCG ATC
Details for ButhA.12800.r.A1.GE34789
HARVESTED ON: 3/13/2012
SEQUENCED ON: 3/12/2012
EXPECTED MW: 37kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 78
PERCENT COVERAGE: 106
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTIDAHNLN DLMYFSQVVE HGGFSAAERV LGISKSRLSR RLTELEATLG VRLLQRSTRK LALTEAGQLF YQHCQAMLSE AQAAMNAVQQ LRSAPRGTVR VSVPVTLSQT MLSRLLPEFL RRFPEVRVQI RVTNRVIDLF EDSIDVALRV RSEPPQNANI VVRPLWRTEQ MLVGAPSLLQ QNAPPLVPDD LSRFDTLDTP SGDGRHVFNL IAPDGTRHAH EHEPRLVTAD LMTIREAVLX XSESPRCRNR CTARIACGAI VAXDAGXDAA RAAXLXGVRV AXGXAACRXX XRRLPXSXXS XXXXXXXVXX XXXXXXXXXX FXXXXXXXXX XXXXXXXXXX XXXXX
Validated NT Sequence
tannnntttt nnttacttta agaaggagan ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gactatcgat gcacataacc tgaacgactt gatgtacttc tctcaggtag tcgagcatgg cggcttctcg gccgcggaac gcgtgctcgg catctcgaag tcgcgcctgt cgcgacggct gaccgagctc gaggcgacgc tcggtgtgcg cctgttgcag cgctcgaccc gcaagctcgc gttgaccgag gcgggccagc ttttctatca gcattgccaa gcaatgttgt cggaggcgca ggccgcgatg aacgcggtgc agcaactgcg ctcggcgccg cgcggcacgg tgcgcgtgag cgtgcccgtc acgctgtcgc agacgatgct gtcgcgcctg ctgccggaat tcctgcgccg ctttccggaa gtgcgcgtgc agattcgcgt gacgaatcgc gtgatcgacc tgtttgaaga ttcgatcgac gtcgcgctgc gcgtgcgatc cgagccgccg cagaatgcga acatcgtcgt gcggccgctg tggcgtaccg agcagatgct cgttggcgcg ccgagcctgc tgcagcagaa cgcgccgcct ctcgtgcccg acgatctgtc gagattcgat acgctcgata cgccgtcagg cgacgggcgg cacgtattca acctgatcgc ccccgacggc acgcgccatg cgcacgagca cgagccgcgg ctcgtgaccg ccgatctgat gacgattcgc gaagccgtgc tcgannggtc ggaatcgccg cgctgccgga atcgatgtac ggcacgcatt gcgtgcgggg caattgtcgc nnnngatgcc gggntggacg ctgcccgtgc cgcanctctn tgnggngttc gtgtcgcnnn ngggnangcc gcctgccgng nnnnnnttcg tcgattaccn ncntcnanaa nctcgnnnnn nnnngnntan nnngnagtcn agntgncnnn nnnnnngcnn nnangnnnnn annntttcgn nnnnnnaaan nnnnnnannn ntnncnnntn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntt t
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTIDAHNLN DLMYFSQVVE HGGFSAAERV LGISKSRLSR RLTELEATLG VRLLQRSTRK LALTEAGQLF YQHCQAMLSE AQAAMNAVQQ LRSAPRGTVR VSVPVTLSQT MLSRLLPEFL RRFPEVRVQI RVTNRVIDLF EDSIDVALRV RSEPPQNANI VVRPLWRTEQ MLVGAPSLLQ QNAPPLVPDD LSRFDTLDTP SGDGRHVFNL IAPDGTRHAH EHEPRLVTAD LMTIREAVLD GVGIAALPES MYGNALRAGQ LSPVMPGWTL PVPQLYAVFV SRQGMPPAVR AFVDYLVEKL DSADYKESSC PERGAKDAKI SI
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gactatcgat gcacataacc tgaacgactt gatgtacttc tctcaggtag tcgagcatgg cggcttctcg gccgcggaac gcgtgctcgg catctcgaag tcgcgcctgt cgcgacggct gaccgagctc gaggcgacgc tcggtgtgcg cctgttgcag cgctcgaccc gcaagctcgc gttgaccgag gcgggccagc ttttctatca gcattgccaa gcaatgttgt cggaggcgca ggccgcgatg aacgcggtgc agcaactgcg ctcggcgccg cgcggcacgg tgcgcgtgag cgtgcccgtc acgctgtcgc agacgatgct gtcgcgcctg ctgccggaat tcctgcgccg ctttccggaa gtgcgcgtgc agattcgcgt gacgaatcgc gtgatcgacc tgtttgaaga ttcgatcgac gtcgcgctgc gcgtgcgatc cgagccgccg cagaatgcga acatcgtcgt gcggccgctg tggcgtaccg agcagatgct cgttggcgcg ccgagcctgc tgcagcagaa cgcgccgcct ctcgtgcccg acgatctgtc gagattcgat acgctcgata cgccgtcagg cgacgggcgg cacgtattca acctgatcgc ccccgacggc acgcgccatg cgcacgagca cgagccgcgg ctcgtgaccg ccgatctgat gacgattcgc gaagccgtgc tcgacggggt cggaatcgcc gcgctgccgg aatcgatgta cggcaacgca ttgcgtgcgg ggcaattgtc gccggtgatg ccgggatgga cgctgcccgt gccgcagctc tatgcggtgt tcgtgtcgcg gcaggggatg ccgcctgccg tgcgcgcgtt cgtcgattac ctcgtcgaga agctcgacag cgcggactac aaggagtcga gttgcccgga gcgcggcgcg aaggacgcga agatttcgat ctaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa