SSGCID: Target Page

ButhA.12800.o

Transcriptional regulator

CENTER ID:	ButhA.12800.o
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.12800.o.A1.GE34777	Full length( ButhA.12800.o )		1	296

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.2383
RefSeq:	YP_440345.1
UniProt:	Q2T3A4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDLNDVRIFV GVVQTGSLSG AAARLEMPLP TVSRRIRALE KQLRVQLLER TARGTRLTEA GTRLYEHASR GVEILAEGEQ AVVSDQARLK GRLRLSLPPA FEAWWELLSA FQKRYPDIRV HAYATERRVD LIEDGIDVAL RVGAIAHEAM VARALLAYRH VLVASPSLVD ALGIPEVPDD LHRFPCAIWS SAANFSRGWR LGDRTFEPDA ALSTNDYMHL RSRAIAGEVV TELPPFLAAE PIRQGRLTAL LCAYPLPEQH VNLLYPSHRH PSAIVRAYLE FCQSQVAWLE HVCAIR

NT Sequence

TTGGATCTCA ATGATGTCCG GATTTTTGTC GGCGTCGTGC AGACGGGCAG CCTTTCTGGT GCCGCGGCGC GACTGGAGAT GCCGTTGCCG ACGGTCAGCC GTCGCATTCG CGCGCTCGAG AAGCAACTGC GGGTTCAGTT GCTGGAGCGC ACGGCGCGCG GCACCCGGCT GACCGAGGCG GGGACCCGGC TCTACGAGCA CGCGAGCCGA GGCGTCGAGA TACTCGCGGA GGGCGAGCAG GCGGTGGTGA GCGATCAAGC TCGGCTGAAG GGGCGGCTGC GCCTGTCGTT GCCGCCCGCG TTCGAGGCGT GGTGGGAGCT ATTGTCCGCG TTCCAGAAGC GCTATCCGGA CATTCGCGTG CACGCATATG CCACCGAGCG TCGAGTGGAT CTGATCGAAG ACGGGATCGA CGTCGCGCTG CGCGTGGGCG CGATCGCGCA TGAGGCGATG GTCGCACGCG CGTTGCTTGC GTATCGCCAT GTCCTGGTCG CCAGTCCGAG TCTGGTCGAT GCGCTGGGCA TTCCCGAGGT GCCTGACGAT TTGCACCGCT TCCCCTGCGC GATATGGAGT TCGGCGGCCA ATTTCAGCCG AGGATGGCGC CTTGGCGATC GAACGTTCGA ACCTGACGCA GCCCTTTCCA CCAACGATTA CATGCACTTG CGCAGCCGCG CGATCGCCGG CGAGGTCGTC ACCGAGCTTC CGCCGTTTCT GGCCGCAGAG CCGATTCGGC AGGGGAGGTT GACCGCTTTG CTTTGCGCTT ACCCGCTGCC CGAGCAGCAC GTCAACTTGC TGTATCCATC GCATCGGCAT CCTTCGGCGA TCGTTCGCGC CTATCTCGAG TTTTGCCAAT CGCAGGTTGC ATGGCTTGAG CATGTCTGTG CGATCCGC

Details for ButhA.12800.o.A1.GE34777

HARVESTED ON:	3/13/2012
SEQUENCED ON:	3/12/2012
EXPECTED MW:	35kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	96
PERCENT COVERAGE:	113

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMLDLNDVRI FVGVVQTGSL SGAAARLEMP LPTVSRRIRA LEKQLRVQLL ERTARGTRLT EAGTRLYEHA SRGVEILAEG EQAVVSDQAR LKGRLRLSLP PAFEAWWELL SAFQKRYPDI RVHAYATERR VDLIEDGIDV ALRVGAIAHE AMVARALLAY RHVLVASPSL VDALGIPEVP DDLHRFPCAI WSSAANFSRG WRLGDRTFEP DAALSTNDYM HLRSRAIAGE VVTELPPFLA AEPIRQGRLT ALLCAYPLPE QHVNLLYPSH RHPSAIVRXX EFCQSXVAWX XXWXXXIXXX XXXXXSAAXL XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XX

Validated NT Sequence

attttnntnn ntttaagaag gaganatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgttgg atctcaatga tgtccggatt tttgtcggcg tcgtgcagac gggcagcctt tctggtgccg cggcgcgact ggagatgccg ttgccgacgg tcagccgtcg cattcgcgcg ctcgagaagc aactgcgggt tcagttgctg gagcgcacgg cgcgcggcac ccggctgacc gaggcgggga cccggctcta cgagcacgcg agccgaggcg tcgagatact cgcggagggc gagcaggcgg tggtgagcga tcaagctcgg ctgaaggggc ggctgcgcct gtcgttgccg cccgcgttcg aggcgtggtg ggagctattg tccgcgttcc agaagcgcta tccggacatt cgcgtgcacg catatgccac cgagcgtcga gtggatctga tcgaagacgg gatcgacgtc gcgctgcgcg tgggcgcgat cgcgcatgag gcgatggtcg cacgcgcgtt gcttgcgtat cgccatgtcc tggtcgccag tccgagtctg gtcgatgcgc tgggcattcc cgaggtgcct gacgatttgc accgcttccc ctgcgcgata tggagttcgg cggccaattt cagccgagga tggcgccttg gcgatcgaac gttcgaacct gacgcagccc tttccaccaa cgattacatg cacttgcgca gccgcgcgat cgccggcgag gtcgtcaccg agcttccgcc gtttctggcc gcagagccga ttcggcaggg gaggttgacc gctttgcttt gcgcttaccc gctgccngag cagcacgtca acttgctgta tccatcgcat cggcatcctt cggcgatcgt tcgnnccnat gagttttgcc aatcgnaggt tgcatggntn gnnncatggn cnnnnnnnat ccncnannna nnnncnngaa nnanttctgc agcnnagctn nnnnnggnnc nnnnnnnnnn cncccnnnnn nnnnnnnnnn nnnnnnnnnn nannnnnntt tnnnnnnntt nnnnnnnnnn nnnnannnnn nnnnnng

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttggatctc aatgatgtcc ggatttttgt cggcgtcgtg cagacgggca gcctttctgg tgccgcggcg cgactggaga tgccgttgcc gacggtcagc cgtcgcattc gcgcgctcga gaagcaactg cgggttcagt tgctggagcg cacggcgcgc ggcacccggc tgaccgaggc ggggacccgg ctctacgagc acgcgagccg aggcgtcgag atactcgcgg agggcgagca ggcggtggtg agcgatcaag ctcggctgaa ggggcggctg cgcctgtcgt tgccgcccgc gttcgaggcg tggtgggagc tattgtccgc gttccagaag cgctatccgg acattcgcgt gcacgcatat gccaccgagc gtcgagtgga tctgatcgaa gacgggatcg acgtcgcgct gcgcgtgggc gcgatcgcgc atgaggcgat ggtcgcacgc gcgttgcttg cgtatcgcca tgtcctggtc gccagtccga gtctggtcga tgcgctgggc attcccgagg tgcctgacga tttgcaccgc ttcccctgcg cgatatggag ttcggcggcc aatttcagcc gaggatggcg ccttggcgat cgaacgttcg aacctgacgc agccctttcc accaacgatt acatgcactt gcgcagccgc gcgatcgccg gcgaggtcgt caccgagctt ccgccgtttc tggccgcaga gccgattcgg caggggaggt tgaccgcttt gctttgcgct tacccgctgc ccgagcagca cgtcaacttg ctgtatccat cgcatcggca tccttcggcg atcgttcgcg cctatctcga gttttgccaa tcgcaggttg catggcttga gcatgtctgt gcgatccgct aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa