SSGCID: Target Page

ButhA.12800.n

LysR family regulatory protein

CENTER ID:	ButhA.12800.n
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.12800.n.A1.GE34788	Full length( ButhA.12800.n )		1	320

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.2966
RefSeq:	YP_440864.1
UniProt:	Q2T1T5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDTLVSMNVF RYVVEVGSFV GAAERMEMSA AMASKHVMHL EHQLGARLLH RTTRRVAPTE AGREYYERLV QALTELDEAG QAVGAASVVP QGRLRVTSLS AFGLRHVMDA VTSYAQRYAD VTVEITLSDR VVELIDEGYD VAVRAAPAGM KSSSLVARQI ATAHILLVAS PDYLAKHGTP TTIADLQHHS FIRREPNETL LDAAISDVAA ASRVPLSGNV IVNHLEGLRV AVLDGAGIAL LGTEVVGEDI ESGRLVPLLL DALPPRELPI YAVYASRRHV SAKVRSFVDF LADRFAGQSL CPSIDERLRQ FAMPRMKRLG

NT Sequence

ATGGATACCC TCGTCAGCAT GAATGTGTTC CGCTATGTCG TCGAAGTCGG CAGCTTCGTC GGCGCGGCCG AGCGCATGGA GATGTCGGCT GCGATGGCGA GCAAGCATGT GATGCATCTC GAGCATCAGC TCGGCGCGCG TCTCTTGCAT CGCACGACGC GCCGCGTCGC GCCGACGGAG GCGGGACGCG AATACTACGA GCGGCTCGTG CAGGCGCTCA CCGAACTCGA CGAGGCCGGG CAGGCGGTGG GCGCCGCGAG CGTCGTGCCA CAAGGGCGGC TGCGCGTGAC GTCGCTGTCC GCGTTCGGGC TGCGGCACGT GATGGACGCG GTGACGTCGT ACGCGCAGCG CTACGCCGAC GTGACGGTCG AGATCACGCT GTCCGACCGC GTCGTCGAGC TGATCGACGA AGGCTACGAC GTCGCGGTGC GCGCGGCGCC CGCCGGCATG AAGTCGTCGT CGCTCGTCGC GCGGCAGATC GCGACCGCGC ACATCCTGCT CGTCGCTTCG CCCGACTATC TCGCGAAGCA CGGCACGCCG ACGACGATCG CCGATCTTCA GCATCACAGC TTCATTCGCC GCGAGCCGAA CGAGACGCTG CTCGACGCGG CGATCTCCGA CGTCGCGGCC GCCTCGCGCG TGCCGCTGTC GGGCAACGTG ATCGTCAATC ATCTCGAAGG GCTGCGCGTC GCGGTGCTGG ACGGCGCGGG CATCGCGCTC CTCGGCACCG AAGTGGTGGG CGAGGACATC GAGTCCGGCC GTCTCGTGCC GTTGCTGCTC GATGCGCTGC CGCCGCGCGA GCTGCCGATC TACGCGGTGT ACGCGAGCCG CCGGCACGTA TCGGCGAAGG TGCGCTCGTT CGTCGATTTC CTCGCCGATC GCTTCGCCGG CCAGTCGCTG TGCCCGTCGA TCGACGAGCG GTTGCGGCAG TTCGCGATGC CGCGGATGAA GCGGCTCGGA

Details for ButhA.12800.n.A1.GE34788

HARVESTED ON:	3/13/2012
SEQUENCED ON:	3/12/2012
EXPECTED MW:	37kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	87
PERCENT COVERAGE:	101

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMDTLVSMNV FRYVVEVGSF VGAAERMEMS AAMASKHVMH LEHQLGARLL HRTTRRVAPT EAGREYYERL VQALTELDEA GQAVGAASVV PQGRLRVTSL SAFGLRHVMD AVTSYAQRYA DVTVEITLSD RVVELIDEGY DVAVRAAPAG MKSSSLVARQ IATAHILLVA SPDYLAKHGT PTTIADLQHH SFIRREPNET LLDAAISDVA AASRVPLSGN VIVNHLEGLR VAVLXGAGIA LLGTEVVGED IESGRLVPLL LDALPPRELP IYAXXASRXX VSXXXXXSSI SSXIAXPVXX XVDRXXXXXX XXXXXXXXXX XXXXXXX

Validated NT Sequence

cnnnnnnnat tttnnttact ttaagaagga ganataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggatacc ctcgtcagca tgaatgtgtt ccgctatgtc gtcgaagtcg gcagcttcgt cggcgcggcc gagcgcatgg agatgtcggc tgcgatggcg agcaagcatg tgatgcatct cgagcatcag ctcggcgcgc gtctcttgca tcgcacgacg cgccgcgtcg cgccgacgga ggcgggacgc gaatactacg agcggctcgt gcaggcgctc accgaactcg acgaggccgg gcaggcggtg ggcgccgcga gcgtcgtgcc acaagggcgg ctgcgcgtga cgtcgctgtc cgcgttcggg ctgcggcacg tgatggacgc ggtgacgtcg tacgcgcagc gctacgccga cgtgacggtc gagatcacgc tgtccgaccg cgtcgtcgag ctgatcgacg aaggctacga cgtcgcggtg cgcgcggcgc ccgccggcat gaagtcgtcg tcgctcgtcg cgcggcagat cgcgaccgcg cacatcctgc tcgtcgcttc gcccgactat ctcgcgaagc acggcacgcc gacgacgatc gccgatcttc agcatcacag cttcattcgc cgcgagccga acgagacgct gctcgacgcg gcgatctccg acgtcgcggc cgcctcgcgc gtgccgctgt cgggcaacgt gatcgtcaat catctcgaag gnctgcgcgt cgcggtgctg ganggngcgg gcatcgcgct cctcggcacc gaagtggtgg gcgaggacat cgagtccggc cgtctcgtgc cgttgctgct cgatgcgctg ccgccgcgcg agctgccgat ctacgcggng nacgcnagcc gcnngnncgt atcggnnann ngcnctcntt cgtcnatttc ctcnncnatc gcttnnccag tnnntgnnnc cgtcgatcga cnannngnnn nngnanntnn nnatncnnga tnnannnnnn tcnnnnnnnn naannnnnnn annnanttnn t

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMDTLVSMNV FRYVVEVGSF VGAAERMEMS AAMASKHVMH LEHQLGARLL HRTTRRVAPT EAGREYYERL VQALTELDEA GQAVGAASVV PQGRLRVTSL SAFGLRHVMD AVTSYAQRYA DVTVEITLSD RVVELIDEGY DVAVRAAPAG MKSSSLVARQ IATAHILLVA SPDYLAKHGT PTTIADLQHH SFIRREPNET LLDAAISDVA AASRVPLSGN VIVNHLEGLR VAVLDGAGIA LLGTEVVGED IESGRLVPLL LDALPPRELP IYAVYASRRH VSAKVRSFVD FLADRFAGQS LCPSIDERLR QFAMPRMKRL G

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggataccctc gtcagcatga atgtgttccg ctatgtcgtc gaagtcggca gcttcgtcgg cgcggccgag cgcatggaga tgtcggctgc gatggcgagc aagcatgtga tgcatctcga gcatcagctc ggcgcgcgtc tcttgcatcg cacgacgcgc cgcgtcgcgc cgacggaggc gggacgcgaa tactacgagc ggctcgtgca ggcgctcacc gaactcgacg aggccgggca ggcggtgggc gccgcgagcg tcgtgccaca agggcggctg cgcgtgacgt cgctgtccgc gttcgggctg cggcacgtga tggacgcggt gacgtcgtac gcgcagcgct acgccgacgt gacggtcgag atcacgctgt ccgaccgcgt cgtcgagctg atcgacgaag gctacgacgt cgcggtgcgc gcggcgcccg ccggcatgaa gtcgtcgtcg ctcgtcgcgc ggcagatcgc gaccgcgcac atcctgctcg tcgcttcgcc cgactatctc gcgaagcacg gcacgccgac gacgatcgcc gatcttcagc atcacagctt cattcgccgc gagccgaacg agacgctgct cgacgcggcg atctccgacg tcgcggccgc ctcgcgcgtg ccgctgtcgg gcaacgtgat cgtcaatcat ctcgaagggc tgcgcgtcgc ggtgctggac ggcgcgggca tcgcgctcct cggcaccgaa gtggtgggcg aggacatcga gtccggccgt ctcgtgccgt tgctgctcga tgcgctgccg ccgcgcgagc tgccgatcta cgcggtgtac gcgagccgcc ggcacgtatc ggcgaaggtg cgctcgttcg tcgatttcct cgccgatcgc ttcgccggcc agtcgctgtg cccgtcgatc gacgagcggt tgcggcagtt cgcgatgccg cggatgaagc ggctcggata aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a