ButhA.12800.m

Transcriptional regulator, LysR family

CENTER ID: ButhA.12800.m
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.12800.m.A2.GE32061 Mature protein( ButhA.12800.m ) 29 304
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.3060
RefSeq: YP_440946.1
UniProt: Q2T1K3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDRLTSMAVF VKAAETGSFA AAGASLGLSS QMVGKHVRFL ETHYGMRLIN RTTRRQSLTE AGRAFYERSR AVLAEAQAVD ALAANLMAAP RGTLRINAPI SFGVHCLMPM LASYMRAYPE VQIDLTLSDR IVDVVDEGYD AVIRIAPLAD SGLIARPLAP YRLVLCASPA YLAEHGAPAE PADLAAHECL GFSYSGQPAA LEWPFESAQG QQHSVRVRSR LAVNNGAALR MAALDGLGII LQPAELVRDD LAAGRLVRLL PGHEPPARPI HVLYSPDRQP TPKLRSFIDA VIAAFGERAG DGRT
NT Sequence
ATGGATCGAC TCACCAGCAT GGCTGTGTTC GTGAAGGCCG CGGAAACGGG CTCGTTCGCG GCGGCGGGCG CATCGCTCGG CCTGTCGTCG CAGATGGTCG GCAAGCACGT GCGCTTTCTC GAGACCCACT ACGGGATGCG GCTCATCAAT CGCACGACGC GCCGCCAGAG CCTGACCGAA GCCGGCCGCG CGTTCTACGA GCGCAGCCGG GCGGTACTGG CCGAGGCGCA GGCCGTCGAC GCGCTCGCCG CGAACCTGAT GGCCGCGCCG CGCGGCACGC TGCGCATCAA CGCTCCGATC AGCTTCGGCG TGCATTGCCT GATGCCGATG CTCGCGTCGT ACATGCGCGC GTATCCGGAG GTGCAGATTG ATCTGACGTT GAGCGATCGC ATCGTCGATG TCGTCGACGA AGGCTACGAC GCGGTGATCC GCATCGCGCC GCTCGCCGAT TCCGGGCTGA TCGCGCGGCC GCTCGCGCCG TACCGGCTCG TGCTGTGCGC GTCGCCCGCG TATCTCGCCG AGCACGGCGC GCCGGCCGAG CCCGCCGATC TGGCCGCGCA CGAATGCCTC GGGTTTTCGT ATTCGGGGCA GCCGGCGGCG CTCGAATGGC CGTTCGAGAG CGCGCAGGGG CAGCAGCATT CGGTGCGCGT GAGGAGCCGG CTCGCGGTCA ACAACGGTGC GGCGCTGCGG ATGGCCGCGC TCGACGGGCT CGGCATCATC CTGCAGCCGG CCGAGCTCGT GCGCGACGAT CTCGCGGCGG GCCGGCTCGT GCGCCTGCTG CCCGGCCATG AGCCGCCCGC GCGGCCGATC CATGTGCTGT ATTCGCCCGA TCGGCAGCCG ACGCCGAAGC TGCGCAGCTT CATCGACGCC GTGATCGCGG CGTTCGGCGA GCGGGCGGGC GACGGGCGGA CG
Details for ButhA.12800.m.A2.GE32061
HARVESTED ON: 4/19/2011
SEQUENCED ON: 4/25/2011
EXPECTED MW: 32kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 93
PERCENT COVERAGE: 110
Validated AA Sequence
MAXHHHHHMG TLEAQTQGPG SMSSQMVGKH VRFLETHYGM RLINRTTRRQ SLTEAGRAFY ERSRAVLAEA QAVDALAANL MAAPRGTLRI NAPISFGVHC LMPMLASYMR AYPEVQIDLT LSDRIVDVVD EGYDAVIRIA PLADSGLIAR PLAPYRLVLC ASPAYLAEHG APAEPADLAA HECLGFSYSG QPAALEWPFE SAQGQQHSVR VRSRLAVNNG AALRMXALDG LGIILQPAEL VXDDXAAGRL VRLLPGXEPP ARPIHVLYSP DRXPTXXLRX FIDXIAXXGX XGXXXXXXXN XXXTXXXXXX XXXXPXXXXX XXRXXXXX
Validated NT Sequence
gnnnnnnnnn nnnnnnngnn annnnnacca tggctnatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgtcgtc gcagatggtc ggcaagcacg tgcgctttct cgagacccac tacgggatgc ggctcatcaa tcgcacgacg cgccgccaga gcctgaccga agccggccgc gcgttctacg agcgcagccg ggcggtactg gccgaggcgc aggccgtcga cgcgctcgcc gcgaacctga tggccgcgcc gcgcggcacg ctgcgcatca acgctccgat cagcttcggc gtgcattgcc tgatgccgat gctcgcgtcg tacatgcgcg cgtatccgga ggtgcagatt gatctgacgt tgagcgatcg catcgtcgat gtcgtcgacg aaggctacga cgcggtgatc cgcatcgcgc cgctcgccga ttccgggctg atcgcgcggc cgctcgcgcc gtaccggctc gtgctgtgcg cgtcgcccgc gtatctcgcc gagcacggcg cgccggccga gcccgccgat ctggccgcgc acgaatgcct cgggttttcg tattcggggc agccggcggc gctcgaatgg ccgttcgaga gcgcgcaggg gcagcagcat tcggtgcgcg tgaggagccg gctcgcggtc aacaacggtg cggcgctgcg gatggncgcg ctcgacgggc tcggcatcat cctgcagccg gccgagctcg tgngcgacga tntcgcggcg ggncggctcg tgcgcctgct gcccggnnat gagccgcccg cgcggccgat ccatgtgctg tattcncccg atcggnagcc gacgnnnaan ctgcgcanct tcatcgacnt gatcgcnnng ttnggnnann ngggcnggcn annggnggan ntaanaaaca nnnnnnnaac nanntnnnnn nnnncnancn tnnnnnnnnn nccngntnnn nannnaannn cnnaaaggna nnntnannnn nnng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSSQMVGKH VRFLETHYGM RLINRTTRRQ SLTEAGRAFY ERSRAVLAEA QAVDALAANL MAAPRGTLRI NAPISFGVHC LMPMLASYMR AYPEVQIDLT LSDRIVDVVD EGYDAVIRIA PLADSGLIAR PLAPYRLVLC ASPAYLAEHG APAEPADLAA HECLGFSYSG QPAALEWPFE SAQGQQHSVR VRSRLAVNNG AALRMAALDG LGIILQPAEL VRDDLAAGRL VRLLPGHEPP ARPIHVLYSP DRQPTPKLRS FIDAVIAAFG ERAGDGRT
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcgtcgcag atggtcggca agcacgtgcg ctttctcgag acccactacg ggatgcggct catcaatcgc acgacgcgcc gccagagcct gaccgaagcc ggccgcgcgt tctacgagcg cagccgggcg gtactggccg aggcgcaggc cgtcgacgcg ctcgccgcga acctgatggc cgcgccgcgc ggcacgctgc gcatcaacgc tccgatcagc ttcggcgtgc attgcctgat gccgatgctc gcgtcgtaca tgcgcgcgta tccggaggtg cagattgatc tgacgttgag cgatcgcatc gtcgatgtcg tcgacgaagg ctacgacgcg gtgatccgca tcgcgccgct cgccgattcc gggctgatcg cgcggccgct cgcgccgtac cggctcgtgc tgtgcgcgtc gcccgcgtat ctcgccgagc acggcgcgcc ggccgagccc gccgatctgg ccgcgcacga atgcctcggg ttttcgtatt cggggcagcc ggcggcgctc gaatggccgt tcgagagcgc gcaggggcag cagcattcgg tgcgcgtgag gagccggctc gcggtcaaca acggtgcggc gctgcggatg gccgcgctcg acgggctcgg catcatcctg cagccggccg agctcgtgcg cgacgatctc gcggcgggcc ggctcgtgcg cctgctgccc ggccatgagc cgcccgcgcg gccgatccat gtgctgtatt cgcccgatcg gcagccgacg ccgaagctgc gcagcttcat cgacgccgtg atcgcggcgt tcggcgagcg ggcgggcgac gggcggacgt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa