SSGCID: Target Page

ButhA.12800.j

LysR family transcriptional regulator

CENTER ID:	ButhA.12800.j
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.12800.j.A1.GE34787	Full length( ButhA.12800.j )		1	320

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.3518
RefSeq:	YP_441355.1
UniProt:	Q2T0E4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MREVNRFSEM AVFVQVIESC GFSAAGRRLD MTPSAISKFV QRLETRLGVR LLNRTTRKLQ LTPEGAAFYE RCVRILEDIA DAEREATHGA IPQGRVRINS HVPFGKHHLM PLVPEFQRRY PDITLDIVLS DAAAGPLDDR SDIAIRSGSL DDSRLVMRRL GGSRMIVVGS PAYLELNGMP ATPDDLDRHN CIGLSGNAKA WPFAATEGQR LLQWGGNLLL GDNESIREAA VSGLGLARLA RFHVDADLDA GRLVPVLEAF NPGDEEEINA VFVGPSEHLP MRVHALLEFL KERIRIGPRA AGDRSDAPML GARAVVTRLR

NT Sequence

ATGCGCGAGG TCAATCGTTT CTCCGAGATG GCCGTATTCG TTCAGGTGAT CGAGTCGTGC GGATTCTCCG CGGCGGGACG GCGGCTCGAC ATGACGCCGT CGGCGATCAG CAAGTTCGTT CAACGGCTCG AGACGCGGCT TGGCGTGCGC CTGTTGAATC GCACGACGCG CAAGCTGCAG CTCACGCCCG AAGGCGCCGC GTTCTACGAA CGCTGCGTGC GCATCCTCGA AGACATCGCC GACGCCGAAC GCGAAGCGAC GCATGGCGCG ATTCCGCAAG GACGCGTGCG GATCAATTCG CACGTGCCGT TCGGCAAGCA TCATCTGATG CCGCTCGTGC CCGAGTTCCA GCGCCGCTAC CCGGACATCA CGCTCGACAT CGTGCTGTCG GACGCGGCCG CCGGCCCGCT CGACGACCGC AGCGACATCG CGATCCGCTC CGGCTCGCTC GACGATTCGC GGCTCGTGAT GCGCCGGCTC GGCGGCAGCC GGATGATCGT CGTCGGCTCG CCCGCGTATC TCGAGCTCAA CGGCATGCCG GCGACGCCCG ACGATCTCGA TCGCCACAAC TGCATCGGCC TGAGCGGCAA CGCGAAGGCA TGGCCGTTCG CCGCGACGGA AGGCCAGCGC CTGCTGCAGT GGGGCGGCAA CCTGCTGCTC GGCGACAACG AAAGCATCCG CGAAGCCGCC GTCTCCGGGC TCGGGCTCGC GCGGCTCGCG CGCTTTCACG TCGACGCGGA CCTCGACGCG GGGCGCCTCG TGCCGGTGCT CGAAGCATTC AATCCGGGCG ACGAGGAGGA GATCAACGCG GTGTTCGTCG GGCCGAGCGA ACACCTGCCG ATGCGCGTGC ATGCGCTACT CGAGTTCCTG AAGGAGCGGA TACGGATCGG GCCGCGCGCC GCCGGCGATC GCAGCGACGC GCCGATGCTC GGCGCACGCG CCGTCGTCAC GCGGCTGCGC

Details for ButhA.12800.j.A1.GE34787

HARVESTED ON:	3/13/2012
SEQUENCED ON:	3/12/2012
EXPECTED MW:	37kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMREVNRFSE MAVFVQVIES CGFSAAGRRL DMTPSAISKF VQRLETRLGV RLLNRTTRKL QLTPEGAAFY ERCVRILEDI ADAEREATHG AIPQGRVRIN SHVPFGKHHL MPLVPEFQRR YPDITLDIVL SDAAAGPLDD RSDIAIRSGS LDDSRLVMRR LGGSRMIVVG SPAYLELNGM PATPDDLDRH NCIGLSGNAK AWPFAATEGQ RLLQWGGNLL LGDNESIREA AVSGLGLARL ARFHVDADLD AGRLVPVLEA FNPGDEEEIN AVFVGPSEHL PMRVHALLEF LKERIRIGPR AAGDRSDAPM LGARAVVTR

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcgcg aggtcaatcg tttctccgag atggccgtat tcgttcaggt gatcgagtcg tgcggattct ccgcggcggg acggcggctc gacatgacgc cgtcggcgat cagcaagttc gttcaacggc tcgagacgcg gcttggcgtg cgcctgttga atcgcacgac gcgcaagctg cagctcacgc ccgaaggcgc cgcgttctac gaacgctgcg tgcgcatcct cgaagacatc gccgacgccg aacgcgaagc gacgcatggc gcgattccgc aaggacgcgt gcggatcaat tcgcacgtgc cgttcggcaa gcatcatctg atgccgctcg tgcccgagtt ccagcgccgc tacccggaca tcacgctcga catcgtgctg tcggacgcgg ccgccggccc gctcgacgac cgcagcgaca tcgcgatccg ctccggctcg ctcgacgatt cgcggctcgt gatgcgccgg ctcggcggca gccggatgat cgtcgtcggc tcgcccgcgt atctcgagct caacggcatg ccggcgacgc ccgacgatct cgatcgccac aactgcatcg gcctgagcgg caacgcgaag gcatggccgt tcgccgcgac ggaaggccag cgcctgctgc agtggggcgg caacctgctg ctcggcgaca acgaaagcat ccgcgaagcc gccgtctccg ggctcgggct cgcgcggctc gcgcgctttc acgtcgacgc ggacctcgac gcggggcgcc tcgtgccggt gctcgaagca ttcaatccgg gcgacgagga ggagatcaac gcggtgttcg tcgggccgag cgaacacctg ccgatgcgcg tgcatgcgct actcgagttc ctgaaggagc ggatacggat cgggccgcgc gccgccggcg atcgcagcga cgcgccgatg ctcggcgcac gcgccgtcgt cacgcggctg

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgcgaggtc aatcgtttct ccgagatggc cgtattcgtt caggtgatcg agtcgtgcgg attctccgcg gcgggacggc ggctcgacat gacgccgtcg gcgatcagca agttcgttca acggctcgag acgcggcttg gcgtgcgcct gttgaatcgc acgacgcgca agctgcagct cacgcccgaa ggcgccgcgt tctacgaacg ctgcgtgcgc atcctcgaag acatcgccga cgccgaacgc gaagcgacgc atggcgcgat tccgcaagga cgcgtgcgga tcaattcgca cgtgccgttc ggcaagcatc atctgatgcc gctcgtgccc gagttccagc gccgctaccc ggacatcacg ctcgacatcg tgctgtcgga cgcggccgcc ggcccgctcg acgaccgcag cgacatcgcg atccgctccg gctcgctcga cgattcgcgg ctcgtgatgc gccggctcgg cggcagccgg atgatcgtcg tcggctcgcc cgcgtatctc gagctcaacg gcatgccggc gacgcccgac gatctcgatc gccacaactg catcggcctg agcggcaacg cgaaggcatg gccgttcgcc gcgacggaag gccagcgcct gctgcagtgg ggcggcaacc tgctgctcgg cgacaacgaa agcatccgcg aagccgccgt ctccgggctc gggctcgcgc ggctcgcgcg ctttcacgtc gacgcggacc tcgacgcggg gcgcctcgtg ccggtgctcg aagcattcaa tccgggcgac gaggaggaga tcaacgcggt gttcgtcggg ccgagcgaac acctgccgat gcgcgtgcat gcgctactcg agttcctgaa ggagcggata cggatcgggc cgcgcgccgc cggcgatcgc agcgacgcgc cgatgctcgg cgcacgcgcc gtcgtcacgc ggctgcgcta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a