ButhA.12800.i

Transcriptional regulator, LysR family

CENTER ID: ButhA.12800.i
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.12800.i.A1.GE34791 Full length( ButhA.12800.i ) 1 326
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.4180
RefSeq: YP_441949.1
UniProt: Q2SYQ0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNQIQTMRVF VCVAELQSFR RAARKLGVSN ALVTRSIAML ETHLNTRLIH RTTRNLSLTE AGIRYLDGCR ALLEEFDQLE ASVERAVHEP VGTLRVAVSG LLSPGRITPL VDSYRRQYPK VRVQLTLTEG QLDVIEAGYD AGLVTGGRLD GNPALIGHPL MPNTLALCAA PSYIERHGAP HRPDDLIRHA CIALPAGQHA STWRFVDPDH FTHVVSLQPV YTVNSASMVR AATLAGTGIS VLPESYIADA LESGELVRIL GDYRIDDPDT QLSIVYPNRQ FLPARTRSFV EHALYHLGGQ KTETNGHYFM REAGTAKRPD VVAGLQ
NT Sequence
ATGAACCAAA TCCAGACCAT GCGTGTCTTC GTATGTGTTG CCGAACTGCA GAGCTTCCGC CGGGCGGCGC GGAAACTCGG CGTATCGAAT GCGCTCGTCA CGCGTTCGAT CGCGATGCTC GAAACACATC TGAACACGCG TCTCATCCAT CGCACGACAC GCAATCTGTC GCTGACGGAA GCGGGCATCC GCTACCTCGA CGGCTGCCGG GCGCTCCTCG AGGAATTCGA TCAGCTCGAG GCGTCCGTCG AGCGAGCGGT GCACGAGCCC GTCGGCACGT TGCGCGTCGC CGTGTCCGGG CTGCTGTCGC CCGGCCGGAT CACCCCGCTC GTCGACAGCT ACCGGCGCCA GTACCCGAAG GTGCGCGTGC AGTTGACGCT GACCGAGGGG CAGCTCGACG TGATCGAAGC CGGCTACGAC GCGGGCCTCG TCACGGGCGG CCGCCTCGAC GGCAACCCGG CGCTCATCGG TCATCCGCTG ATGCCGAACA CGCTCGCGCT CTGCGCGGCG CCCTCGTACA TCGAGCGGCA CGGCGCGCCG CACCGCCCCG ACGATCTCAT CCGCCACGCG TGCATCGCGC TGCCGGCCGG CCAGCATGCA TCGACCTGGC GCTTCGTCGA TCCGGACCAC TTCACGCACG TCGTGTCGCT GCAGCCGGTC TATACGGTCA ACAGCGCGTC GATGGTGCGC GCGGCGACGC TCGCCGGCAC CGGCATCTCC GTGCTGCCGG AAAGCTACAT CGCCGATGCG CTCGAAAGCG GCGAGCTCGT TCGGATTCTC GGCGATTACC GGATCGACGA TCCCGACACG CAGTTGTCCA TCGTCTATCC GAACCGCCAG TTCCTGCCGG CGCGCACGCG CAGCTTCGTC GAGCACGCGC TGTACCACCT CGGCGGGCAG AAGACCGAGA CGAACGGCCA CTACTTCATG CGCGAAGCCG GCACGGCGAA GCGCCCGGAC GTCGTCGCCG GGCTCCAG
Details for ButhA.12800.i.A1.GE34791
HARVESTED ON: 3/13/2012
SEQUENCED ON: 3/12/2012
EXPECTED MW: 38kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 93
Validated AA Sequence
MNQIQTMRVF VCVAELQSFR RAARKLGVSN ALVTRSIAML ETHLNTRLIH RTTRNLSLTE AGIRYLDGCR ALLEEFDQLE ASVERAVHEP VGTLRVAVSG LLSPGRITPL VDSYRRQYPK VRVQLTLTEG QLDVIEAGYD AGLVTGGRLD GNPALIGHPL MPNTLALCAA PSYIERHGAP HRPDDLIRHA CIALPAGQHA STWRFVDPDH FTHVVSLQPV YTVNSASMVR AATLAGTGIS VLPESYIADA LESGELVRIL GDYRIDDPDT QLSIVYPNRQ FLPARTRSFV EHALYHLGGQ KTETNGHYFM REAGTAKRPD VVAGL
Validated NT Sequence
ggagcccggc gacgacgtcc gggcgcttcg ccgtgccggc ttcgcgcatg aagtagtggc cgttcgtctc ggtcttctgc ccgccgaggt ggtacagcgc gtgctcgacg aagctgcgcg tgcgcgccgg caggaactgg cggttcggat agacgatgga caactgcgtg tcgggatcgt cgatccggta atcgccgaga atccgaacga gctcgccgct ttcgagcgca tcggcgatgt agctttccgg cagcacggag atgccggtgc cggcgagcgt cgccgcgcgc accatcgacg cgctgttgac cgtatagacc ggctgcagcg acacgacgtg cgtgaagtgg tccggatcga cgaagcgcca ggtcgatgca tgctggccgg ccggcagcgc gatgcacgcg tggcggatga gatcgtcggg gcggtgcggc gcgccgtgcc gctcgatgta cgagggcgcc gcgcagagcg cgagcgtgtt cggcatcagc ggatgaccga tgagcgccgg gttgccgtcg aggcggccgc ccgtgacgag gcccgcgtcg tagccggctt cgatcacgtc gagctgcccc tcggtcagcg tcaactgcac gcgcaccttc gggtactggc gccggtagct gtcgacgagc ggggtgatcc ggccgggcga cagcagcccg gacacggcga cgcgcaacgt gccgacgggc tcgtgcaccg ctcgctcgac ggacgcctcg agctgatcga attcctcgag gagcgcccgg cagccgtcga ggtagcggat gcccgcttcc gtcagcgaca gattgcgtgt cgtgcgatgg atgagacgcg tgttcagatg tgtttcgagc atcgcgatcg aacgcgtgac gagcgcattc gatacgccga gtttccgcgc cgcccggcgg aagctctgca gttcggcaac acatacgaag acacgcatgg tctggatttg gttcatcgaa ccaggaccct gggtctgagc ttccag
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNQIQTMRV FVCVAELQSF RRAARKLGVS NALVTRSIAM LETHLNTRLI HRTTRNLSLT EAGIRYLDGC RALLEEFDQL EASVERAVHE PVGTLRVAVS GLLSPGRITP LVDSYRRQYP KVRVQLTLTE GQLDVIEAGY DAGLVTGGRL DGNPALIGHP LMPNTLALCA APSYIERHGA PHRPDDLIRH ACIALPAGQH ASTWRFVDPD HFTHVVSLQP VYTVNSASMV RAATLAGTGI SVLPESYIAD ALESGELVRI LGDYRIDDPD TQLSIVYPNR QFLPARTRSF VEHALYHLGG QKTETNGHYF MREAGTAKRP DVVAGLQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaccaaatc cagaccatgc gtgtcttcgt atgtgttgcc gaactgcaga gcttccgccg ggcggcgcgg aaactcggcg tatcgaatgc gctcgtcacg cgttcgatcg cgatgctcga aacacatctg aacacgcgtc tcatccatcg cacgacacgc aatctgtcgc tgacggaagc gggcatccgc tacctcgacg gctgccgggc gctcctcgag gaattcgatc agctcgaggc gtccgtcgag cgagcggtgc acgagcccgt cggcacgttg cgcgtcgccg tgtccgggct gctgtcgccc ggccggatca ccccgctcgt cgacagctac cggcgccagt acccgaaggt gcgcgtgcag ttgacgctga ccgaggggca gctcgacgtg atcgaagccg gctacgacgc gggcctcgtc acgggcggcc gcctcgacgg caacccggcg ctcatcggtc atccgctgat gccgaacacg ctcgcgctct gcgcggcgcc ctcgtacatc gagcggcacg gcgcgccgca ccgccccgac gatctcatcc gccacgcgtg catcgcgctg ccggccggcc agcatgcatc gacctggcgc ttcgtcgatc cggaccactt cacgcacgtc gtgtcgctgc agccggtcta tacggtcaac agcgcgtcga tggtgcgcgc ggcgacgctc gccggcaccg gcatctccgt gctgccggaa agctacatcg ccgatgcgct cgaaagcggc gagctcgttc ggattctcgg cgattaccgg atcgacgatc ccgacacgca gttgtccatc gtctatccga accgccagtt cctgccggcg cgcacgcgca gcttcgtcga gcacgcgctg taccacctcg gcgggcagaa gaccgagacg aacggccact acttcatgcg cgaagccggc acggcgaagc gcccggacgt cgtcgccggg ctccagtaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa