ButhA.12800.h

Transcriptional regulator, LysR family

CENTER ID: ButhA.12800.h
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.12800.h.A1.GE34781 Full length( ButhA.12800.h ) 1 303
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.4532
RefSeq: YP_442278.1
UniProt: Q2SXS1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRDLNDLYYF VQVVDHGGFA AAGRALNMPK SKLSRRVALL EERIGMRLIQ RSTRRFAVTD VGQTYCAHCR AMLVEADAAD EAVALMRAEP RGIVRMTCPV ALLDALVGDM IAAFMAECPL VEIHLEETNR RVDVVGEGID VALRVRPPPL EDSELVLRVL GERGQCLVAS PRLLARAGAG VPRVPADLAA LPSLALGLPQ DEHVWNLYGP DGAHAAIHHR PRLVTRGMLA LRAAAVAGVG VVQLPTMMVR EPIARGELRQ LLPGWAPRRE IVHAVFASRR GLLPSVRALL DFLAQRFAAL EPD
NT Sequence
ATGCGGGACC TGAACGATCT CTATTACTTC GTGCAAGTGG TCGACCACGG CGGCTTCGCG GCCGCCGGGC GCGCGCTGAA CATGCCGAAG TCGAAGCTGA GCCGGCGGGT CGCGCTCCTC GAGGAGCGGA TCGGCATGCG CCTCATCCAG CGCTCGACGC GACGCTTCGC AGTCACGGAT GTCGGCCAGA CCTATTGCGC GCATTGCCGC GCGATGCTCG TCGAGGCGGA CGCGGCCGAC GAAGCGGTCG CGCTGATGCG CGCGGAGCCT CGCGGCATCG TCCGGATGAC CTGCCCCGTC GCGCTCCTCG ATGCGCTCGT CGGCGACATG ATCGCGGCGT TCATGGCCGA ATGCCCGCTC GTCGAGATCC ATCTGGAGGA AACGAACCGC CGCGTCGACG TCGTCGGCGA AGGAATCGAC GTCGCGCTGC GCGTTCGGCC GCCGCCGCTC GAAGACAGCG AGCTCGTGCT GCGCGTGCTC GGCGAGCGCG GACAATGCCT CGTTGCGAGC CCGCGGCTTC TCGCGCGCGC CGGCGCCGGC GTGCCGCGCG TGCCCGCCGA TCTCGCCGCG CTGCCGAGCC TCGCGCTCGG CCTGCCTCAG GACGAACACG TCTGGAACCT GTACGGGCCC GACGGCGCGC ATGCGGCGAT CCATCATCGG CCCCGGCTCG TCACGCGCGG GATGCTCGCG CTGCGCGCGG CGGCCGTCGC GGGGGTCGGC GTCGTTCAGT TGCCGACGAT GATGGTTCGC GAGCCGATCG CGCGCGGCGA GCTGCGGCAA CTGCTGCCCG GCTGGGCCCC GCGACGCGAG ATCGTGCATG CGGTGTTCGC GTCGCGCCGC GGGCTGCTGC CTTCGGTGCG CGCGCTGCTC GACTTTCTCG CGCAGCGTTT CGCCGCGCTC GAGCCGGAT
Details for ButhA.12800.h.A1.GE34781
HARVESTED ON: 3/13/2012
SEQUENCED ON: 3/12/2012
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 87
PERCENT COVERAGE: 102
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRDLNDLYY FVQVVDHGGF AAAGRALNMP KSKLSRRVAL LEERIGMRLI QRSTRRFAVT DVGQTYCAHC RAMLVEADAA DEAVALMRAE PRGIVRMTCP VALLDALVGD MIAAFMAECP LVEIHLEETN RRVDVVGEGI DVALRVRPPP LEDSELVLRV LGERGQCLVA SPRLLARAGA GVPRVPADLA ALPSLALGLP XDEHVWNLYG PDGAHAAIHH RPRLVXRGML ALRAAAVAGV GVVQLPTMMV REPIXRGXLX QLLXGWAPXT XXRAXXXXXX XAXXXXXCST FXXXVPXXXX LXXXXXXFCX X
Validated NT Sequence
ttttnnnnnn tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgcggga cctgaacgat ctctattact tcgtgcaagt ggtcgaccac ggcggcttcg cggccgccgg gcgcgcgctg aacatgccga agtcgaagct gagccggcgg gtcgcgctcc tcgaggagcg gatcggcatg cgcctcatcc agcgctcgac gcgacgcttc gcagtcacgg atgtcggcca gacctattgc gcgcattgcc gcgcgatgct cgtcgaggcg gacgcggccg acgaagcggt cgcgctgatg cgcgcggagc ctcgcggcat cgtccggatg acctgccccg tcgcgctcct cgatgcgctc gtcggcgaca tgatcgcggc gttcatggcc gaatgcccgc tcgtcgagat ccatctggag gaaacgaacc gccgcgtcga cgtcgtcggc gaaggaatcg acgtcgcgct gcgcgttcgg ccgccgccgc tcgaagacag cgagctcgtg ctgcgcgtgc tcggcgagcg cggacaatgc ctcgttgcga gcccgcggct tctcgcgcgc gccggcgccg gcgtgccgcg cgtgcccgcc gatctcgccg cgctgccgag cctcgcgctc ggcctgcctc angacgaaca cgtctggaac ctgtacgggc ccgacggcgc gcatgcggcg atccatcatc ggccccggct cgtcncgcgc gggatgctcg cgctgcgcgc ggcggccgtc gcgggggtcg gcgtcgttca gttgccgacg atgatggttc gcgagccgat cgngcgcggn nagctnnngc aactgctnnc cggntgggcc ccnnnnacnn nagntcgtgc atgnngnnnt nnnntcncgn ntgctncntn nngcnngngc tgctcgactt tctnnnncan cgttccnnnn tngancnnat tantaaanng cncgannnag ttctgcancn agct
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRDLNDLYY FVQVVDHGGF AAAGRALNMP KSKLSRRVAL LEERIGMRLI QRSTRRFAVT DVGQTYCAHC RAMLVEADAA DEAVALMRAE PRGIVRMTCP VALLDALVGD MIAAFMAECP LVEIHLEETN RRVDVVGEGI DVALRVRPPP LEDSELVLRV LGERGQCLVA SPRLLARAGA GVPRVPADLA ALPSLALGLP QDEHVWNLYG PDGAHAAIHH RPRLVTRGML ALRAAAVAGV GVVQLPTMMV REPIARGELR QLLPGWAPRR EIVHAVFASR RGLLPSVRAL LDFLAQRFAA LEPD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgggacctg aacgatctct attacttcgt gcaagtggtc gaccacggcg gcttcgcggc cgccgggcgc gcgctgaaca tgccgaagtc gaagctgagc cggcgggtcg cgctcctcga ggagcggatc ggcatgcgcc tcatccagcg ctcgacgcga cgcttcgcag tcacggatgt cggccagacc tattgcgcgc attgccgcgc gatgctcgtc gaggcggacg cggccgacga agcggtcgcg ctgatgcgcg cggagcctcg cggcatcgtc cggatgacct gccccgtcgc gctcctcgat gcgctcgtcg gcgacatgat cgcggcgttc atggccgaat gcccgctcgt cgagatccat ctggaggaaa cgaaccgccg cgtcgacgtc gtcggcgaag gaatcgacgt cgcgctgcgc gttcggccgc cgccgctcga agacagcgag ctcgtgctgc gcgtgctcgg cgagcgcgga caatgcctcg ttgcgagccc gcggcttctc gcgcgcgccg gcgccggcgt gccgcgcgtg cccgccgatc tcgccgcgct gccgagcctc gcgctcggcc tgcctcagga cgaacacgtc tggaacctgt acgggcccga cggcgcgcat gcggcgatcc atcatcggcc ccggctcgtc acgcgcggga tgctcgcgct gcgcgcggcg gccgtcgcgg gggtcggcgt cgttcagttg ccgacgatga tggttcgcga gccgatcgcg cgcggcgagc tgcggcaact gctgcccggc tgggccccgc gacgcgagat cgtgcatgcg gtgttcgcgt cgcgccgcgg gctgctgcct tcggtgcgcg cgctgctcga ctttctcgcg cagcgtttcg ccgcgctcga gccggattaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa