SSGCID: Target Page

ButhA.12800.g

Transcriptional regulator, LysR family

CENTER ID:	ButhA.12800.g
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.12800.g.A1.GE32055	Full length( ButhA.12800.g )		1	327

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.4803
RefSeq:	YP_442520.1
UniProt:	Q2SX29

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MIFYFSISNL ERLQIRRGGR VAPPEHGMDR FKQIETFAAV AAKGSLSAAA HAEGVAPAII GRRLDALEER LGVKLLVRTT RKLTLTFEGS AFLEDCQRII NDMQNAEASV SAGGVKASGH LRVSAPAGFG RRHVAPLVPA FTGAHPDVSV TLDLSDRMVD LVNEGFDCAV RLGELPDSSL VSLKLGENRR VCVASPAYLA RAGMPETLDD LARHNCLALA ANANQQRGWT FVDDGKVVSI RVSGTMECSD GAVLHEWCLE GHGLAWRSWW EVGADIAAGR LVSVLDAFAA PPIGIHAVFP QRRHLPLRVR LFLDFLKHTY ERPGYWG

NT Sequence

TTGATCTTTT ACTTTTCGAT ATCTAATCTG GAGCGACTTC AGATTCGACG GGGTGGCCGC GTCGCGCCGC CCGAACATGG GATGGATCGC TTCAAACAGA TCGAAACGTT TGCCGCGGTC GCGGCAAAGG GCAGCCTGTC GGCGGCCGCG CACGCGGAAG GGGTCGCGCC CGCGATCATC GGTCGCCGGC TCGACGCGCT CGAGGAGCGG CTCGGCGTGA AGCTGCTCGT GCGCACGACG CGCAAGCTCA CGCTGACGTT CGAAGGCTCG GCGTTCCTCG AGGACTGCCA GCGGATCATC AACGACATGC AGAACGCCGA GGCAAGCGTG TCCGCGGGCG GCGTGAAGGC GAGCGGGCAC CTGCGGGTGT CGGCGCCCGC GGGCTTCGGC CGGCGCCACG TCGCGCCGCT CGTGCCCGCG TTCACGGGCG CGCATCCGGA CGTGTCGGTC ACGCTCGATC TGTCCGACCG GATGGTCGAC CTCGTCAACG AAGGGTTCGA TTGCGCGGTG CGGCTGGGCG AGCTGCCCGA TTCGTCGCTC GTGTCGCTGA AGCTCGGCGA GAACCGGCGC GTGTGTGTCG CGTCGCCCGC GTATCTCGCG CGCGCGGGCA TGCCCGAGAC GCTCGACGAT CTCGCGCGCC ACAACTGCCT CGCGCTCGCG GCGAATGCGA ACCAGCAGCG CGGCTGGACC TTCGTCGACG ATGGCAAGGT GGTGTCGATC CGCGTGTCCG GCACGATGGA GTGCTCGGAC GGCGCGGTGC TGCACGAATG GTGCCTCGAG GGGCATGGCC TGGCCTGGCG TTCGTGGTGG GAAGTCGGCG CGGACATCGC GGCGGGCCGG CTCGTGAGCG TGCTCGACGC GTTCGCCGCG CCGCCGATCG GCATTCACGC GGTGTTTCCG CAGCGCCGGC ACCTGCCGCT CAGGGTCCGG CTCTTTCTCG ATTTTCTGAA GCACACGTAC GAGCGCCCCG GCTACTGGGG A

Details for ButhA.12800.g.A1.GE32055

HARVESTED ON:	4/19/2011
SEQUENCED ON:	4/25/2011
EXPECTED MW:	38kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	81
PERCENT COVERAGE:	104

Validated AA Sequence

MGTLEAQTQG PGSMLIFYFS ISNLERLQIR RGGRVAPPEH GMDRFKQIET FAAVAAKGSL SAAAHAEGVA PAIIGRRLDA LEERLGVKLL VRTTRKLTLT FEGSAFLEDC QRIINDMQNA EASVSAGGVK ASGHLRVSAP AGFGRRHVAP LVPAFTGAHP DVSVTLDLSD RMVDLVNEGF DCAVRLGELP DSSLVSLKLG ENRRVCVASP AYLARAGMPE TLDDLARHNC LALAAXANQQ RGWTFVDDGX VVSIRVXXXD GXLGRRGAAR MVPRGAWXXL XFVXXVXXXI AAGXXVSXXX XFAXXXIGIX XXXXQXXXXX XXXXXXXXXX XXXXXXXXXX GXXXXXXXXX XXXXXXXXXX XXXXXX

Validated NT Sequence

cnnnnnnnnn nnnngtnnnn nnnnnnncct gnnncancac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgttgatc ttttactttt cgatatctaa tctggagcga cttcagattc gacggggtgg ccgcgtcgcg ccgcccgaac atgggatgga tcgcttcaaa cagatcgaaa cgtttgccgc ggtcgcggca aagggcagcc tgtcggcggc cgcgcacgcg gaaggggtcg cgcccgcgat catcggtcgc cggctcgacg cgctcgagga gcggctcggc gtgaagctgc tcgtgcgcac gacgcgcaag ctcacgctga cgttcgaagg ctcggcgttc ctcgaggact gccagcggat catcaacgac atgcagaacg ccgaggcaag cgtgtccgcg ggcggcgtga aggcgagcgg gcacctgcgg gtgtcggcgc ccgcgggctt cggccggcgc cacgtcgcgc cgctcgtgcc cgcgttcacg ggcgcgcatc cggacgtgtc ggtcacgctc gatctgtccg accggatggt cgacctcgtc aacgaagggt tcgattgcgc ggtgcggctg ggcgagctgc ccgattcgtc gctcgtgtcg ctgaagctcg gcgagaaccg gcgcgtgtgt gtcgcgtcgc ccgcgtatct cgcgcgcgcg ggcatgcccg agacgctcga cgatctcgcg cgccacaact gcctcgcgct cgcggcgaan gcgaaccagc agcgcggctg gaccttcgtc gacgatggcn aggtggtgtc gatccgcgtg tncngnnacg atggnntgct cggacggcgc ggngctgcac gaatggtgcc tcgaggggca tggnctgnnc tgncgttcgt ggnnnnagtn nnnncngnna tcgcggcngg ncnnntcgtg agcntgntcg anncnttcgc cgnnnngnnn atcggnattn nnncnnnnnn tnnncagngn nngnnnnnnn nnntnnnnnn nncnnntnnn nnnnnnnttt nnnnnnnnnn nnnnnnnnnn nnnnncnnnn ngggggnnnn tnnnnnnnnn nnnnnnncnn nnnnnnnnnn nnnnnnnnnn cnnannnnnn nnnnnnnnnn nnnnnnnnnn nnc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMLIFYFSIS NLERLQIRRG GRVAPPEHGM DRFKQIETFA AVAAKGSLSA AAHAEGVAPA IIGRRLDALE ERLGVKLLVR TTRKLTLTFE GSAFLEDCQR IINDMQNAEA SVSAGGVKAS GHLRVSAPAG FGRRHVAPLV PAFTGAHPDV SVTLDLSDRM VDLVNEGFDC AVRLGELPDS SLVSLKLGEN RRVCVASPAY LARAGMPETL DDLARHNCLA LAANANQQRG WTFVDDGKVV SIRVSGTMEC SDGAVLHEWC LEGHGLAWRS WWEVGADIAA GRLVSVLDAF AAPPIGIHAV FPQRRHLPLR VRLFLDFLKH TYERPGYWG

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgatcttt tacttttcga tatctaatct ggagcgactt cagattcgac ggggtggccg cgtcgcgccg cccgaacatg ggatggatcg cttcaaacag atcgaaacgt ttgccgcggt cgcggcaaag ggcagcctgt cggcggccgc gcacgcggaa ggggtcgcgc ccgcgatcat cggtcgccgg ctcgacgcgc tcgaggagcg gctcggcgtg aagctgctcg tgcgcacgac gcgcaagctc acgctgacgt tcgaaggctc ggcgttcctc gaggactgcc agcggatcat caacgacatg cagaacgccg aggcaagcgt gtccgcgggc ggcgtgaagg cgagcgggca cctgcgggtg tcggcgcccg cgggcttcgg ccggcgccac gtcgcgccgc tcgtgcccgc gttcacgggc gcgcatccgg acgtgtcggt cacgctcgat ctgtccgacc ggatggtcga cctcgtcaac gaagggttcg attgcgcggt gcggctgggc gagctgcccg attcgtcgct cgtgtcgctg aagctcggcg agaaccggcg cgtgtgtgtc gcgtcgcccg cgtatctcgc gcgcgcgggc atgcccgaga cgctcgacga tctcgcgcgc cacaactgcc tcgcgctcgc ggcgaatgcg aaccagcagc gcggctggac cttcgtcgac gatggcaagg tggtgtcgat ccgcgtgtcc ggcacgatgg agtgctcgga cggcgcggtg ctgcacgaat ggtgcctcga ggggcatggc ctggcctggc gttcgtggtg ggaagtcggc gcggacatcg cggcgggccg gctcgtgagc gtgctcgacg cgttcgccgc gccgccgatc ggcattcacg cggtgtttcc gcagcgccgg cacctgccgc tcagggtccg gctctttctc gattttctga agcacacgta cgagcgcccc ggctactggg gataaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa