SSGCID: Target Page

ButhA.12800.f

Transcriptional regulator, LysR family

CENTER ID:	ButhA.12800.f
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.12800.f.A1.GE32054	Full length( ButhA.12800.f )		1	303

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.4989
RefSeq:	YP_442695.1
UniProt:	Q2SWK4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDRFKQIETF VRVADAGSLA AAALEEGVSP VVLGRRIDAL EKRLGVKLMY RSTRRLVVSE DGAAFLERCR GLLTEWAQAE NEVVAGRRAV GGHLIVSAPA AFGRKHVAPL APAFIADKPD LQMSFNLTDR VVDLVREGYD LSIRIGGAVD PNFVAVKLAS NRRVVCGTPE YFRRHGRPKT LDDLAGHNCL AFNLQGGQNR GWYFRRSGKL VTMRVSGTLD CNDGELLHRW VSEGLGLGWR STWEIAQQLA RGELETVLDE YALPDYDILA VYPQQRYVPA RVRYFIDYLR DVYARPGYWE GAE

NT Sequence

ATGGACCGAT TCAAGCAGAT CGAAACCTTC GTGCGGGTGG CCGACGCGGG CAGCCTCGCG GCGGCCGCGC TCGAGGAGGG CGTGTCGCCC GTCGTGCTCG GGCGGCGGAT CGACGCGCTC GAGAAGCGCC TCGGCGTGAA GCTGATGTAC CGGTCGACGC GCCGGCTCGT CGTGAGCGAG GACGGCGCCG CGTTCCTCGA GCGCTGTCGC GGGCTCCTCA CCGAGTGGGC GCAGGCCGAG AACGAGGTTG TCGCGGGGCG CCGCGCGGTG GGCGGGCATC TGATCGTGTC GGCGCCCGCC GCGTTCGGCC GCAAGCACGT CGCGCCGCTC GCGCCCGCGT TCATCGCCGA CAAGCCCGAC CTGCAGATGT CGTTCAACCT GACCGACCGC GTCGTCGATC TCGTGCGCGA GGGCTACGAT CTGTCGATCC GGATCGGCGG CGCGGTCGAT CCGAACTTCG TCGCGGTGAA GCTCGCGTCG AACCGGCGCG TCGTGTGCGG CACGCCCGAG TATTTTCGCC GGCACGGCCG GCCGAAGACG CTCGACGATC TCGCGGGCCA CAACTGTCTC GCGTTCAATC TGCAGGGCGG CCAGAACCGC GGCTGGTACT TCCGGCGCAG CGGCAAGCTC GTGACGATGC GCGTGTCGGG CACGCTCGAC TGCAACGACG GCGAGCTCCT GCACCGCTGG GTGTCGGAGG GGCTCGGGCT CGGCTGGCGC TCGACGTGGG AGATCGCGCA GCAGCTCGCG CGCGGCGAGC TCGAGACGGT GCTCGACGAG TACGCGCTGC CCGACTACGA CATCCTCGCC GTCTATCCGC AGCAGCGCTA CGTGCCGGCG CGCGTGCGCT ACTTCATCGA TTACTTGCGC GACGTCTATG CGCGGCCGGG GTATTGGGAA GGCGCCGAG

Details for ButhA.12800.f.A1.GE32054

HARVESTED ON:	4/19/2011
SEQUENCED ON:	4/25/2011
EXPECTED MW:	36kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	92

Validated AA Sequence

Validated NT Sequence

ttcccaatac cccggccgcg catagacgtc gcgcaagtaa tcgatgaagt agcgcacgcg cgccggcacg tagcgctgct gcggatagac ggcgaggatg tcgtagtcgg gcagcgcgta ctcgtcgagc accgtctcga gctcgccgcg cgcgagctgc tgcgcgatct cccacgtcga gcgccagccg agcccgagcc cctccgacac ccagcggtgc aggagctcgc cgtcgttgca gtcgagcgtg cccgacacgc gcatcgtcac gagcttgccg ctgcgccgga agtaccagcc gcggttctgg ccgccctgca gattgaacgc gagacagttg tggcccgcga gatcgtcgag cgtcttcggc cggccgtgcc ggcgaaaata ctcgggcgtg ccgcacacga cgcgccggtt cgacgcgagc ttcaccgcga cgaagttcgg atcgaccgcg ccgccgatcc ggatcgacag atcgtagccc tcgcgcacga gatcgacgac gcggtcggtc aggttgaacg acatctgcag gtcgggcttg tcggcgatga acgcgggcgc gagcggcgcg acgtgcttgc ggccgaacgc ggcgggcgcc gacacgatca gatgcccgcc caccgcgcgg cgccccgcga caacctcgtt ctcggcctgc gcccactcgg tgaggagccc gcgacagcgc tcgaggaacg cggcgccgtc ctcgctcacg acgagccggc gcgtcgaccg gtacatcagc ttcacgccga ggcgcttctc gagcgcgtcg atccgccgcc cgagcacgac gggcgacacg ccctcctcga gcgcggccgc cgcgaggctg cccgcgtcgg ccacccgcac gaaggtttcg atctgcttga atcggtccat cgaaccagga ccctgggtct gagcttccag ggtac

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMDRFKQIET FVRVADAGSL AAAALEEGVS PVVLGRRIDA LEKRLGVKLM YRSTRRLVVS EDGAAFLERC RGLLTEWAQA ENEVVAGRRA VGGHLIVSAP AAFGRKHVAP LAPAFIADKP DLQMSFNLTD RVVDLVREGY DLSIRIGGAV DPNFVAVKLA SNRRVVCGTP EYFRRHGRPK TLDDLAGHNC LAFNLQGGQN RGWYFRRSGK LVTMRVSGTL DCNDGELLHR WVSEGLGLGW RSTWEIAQQL ARGELETVLD EYALPDYDIL AVYPQQRYVP ARVRYFIDYL RDVYARPGYW EGAE

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggaccgattc aagcagatcg aaaccttcgt gcgggtggcc gacgcgggca gcctcgcggc ggccgcgctc gaggagggcg tgtcgcccgt cgtgctcggg cggcggatcg acgcgctcga gaagcgcctc ggcgtgaagc tgatgtaccg gtcgacgcgc cggctcgtcg tgagcgagga cggcgccgcg ttcctcgagc gctgtcgcgg gctcctcacc gagtgggcgc aggccgagaa cgaggttgtc gcggggcgcc gcgcggtggg cgggcatctg atcgtgtcgg cgcccgccgc gttcggccgc aagcacgtcg cgccgctcgc gcccgcgttc atcgccgaca agcccgacct gcagatgtcg ttcaacctga ccgaccgcgt cgtcgatctc gtgcgcgagg gctacgatct gtcgatccgg atcggcggcg cggtcgatcc gaacttcgtc gcggtgaagc tcgcgtcgaa ccggcgcgtc gtgtgcggca cgcccgagta ttttcgccgg cacggccggc cgaagacgct cgacgatctc gcgggccaca actgtctcgc gttcaatctg cagggcggcc agaaccgcgg ctggtacttc cggcgcagcg gcaagctcgt gacgatgcgc gtgtcgggca cgctcgactg caacgacggc gagctcctgc accgctgggt gtcggagggg ctcgggctcg gctggcgctc gacgtgggag atcgcgcagc agctcgcgcg cggcgagctc gagacggtgc tcgacgagta cgcgctgccc gactacgaca tcctcgccgt ctatccgcag cagcgctacg tgccggcgcg cgtgcgctac ttcatcgatt acttgcgcga cgtctatgcg cggccggggt attgggaagg cgccgagtaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa