SSGCID: Target Page

ButhA.12800.d

Transcriptional regulator

CENTER ID:	ButhA.12800.d
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.12800.d.A1.GE34785	Full length( ButhA.12800.d )		1	313

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.5481
RefSeq:	YP_443139.1
UniProt:	Q2SVB0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDTLQNMRVF VRVVDAGSFT AAAQQLNTTT AYASRAVSDL EAHLRTRLLN RTTRRIALTE AGERYLQRCE QILAYVEQAE AEASDAHARP SGKLKVHCMT SLGQHYTVPA VSRYRQRYPE VQVDLTLAQR LPDLLDEGFD VSLVVGRELP DSGLVSQRLG ETFSVVCASR GYIETHGAPQ RPQDLAGHVC LGMVAPGLYW DEWKLMGPHG EESVTLAPPP FRVNVAEALA AGIREGMGIG VLPLYSAIAG LRHGDFVRVM PEYRSHVMNI YALYASRQYL DAKIRTWVDF LRDELPSILE ADEAALERFT VQT

NT Sequence

ATGGATACGC TCCAAAACAT GCGTGTGTTC GTTCGCGTGG TCGATGCGGG CAGCTTCACG GCGGCGGCGC AGCAGCTCAA TACGACGACC GCGTACGCGT CGCGCGCGGT GTCCGATCTC GAGGCGCACC TGCGCACGCG TCTTTTGAAC CGTACGACGC GGCGCATCGC GCTCACCGAG GCGGGCGAGC GCTATCTGCA GCGCTGCGAG CAGATCCTCG CATACGTCGA GCAGGCGGAG GCCGAGGCGA GCGACGCGCA TGCGCGCCCG TCCGGCAAGC TGAAGGTGCA CTGCATGACG AGCCTCGGCC AGCACTACAC GGTGCCCGCG GTGTCGCGCT ACCGGCAGCG CTATCCGGAG GTCCAGGTCG ACCTGACGCT CGCGCAGCGG CTGCCGGATC TTCTCGACGA AGGCTTCGAC GTGTCGCTCG TCGTCGGCCG CGAACTGCCC GATTCGGGGC TCGTGTCGCA GCGGCTCGGC GAGACGTTCA GCGTCGTCTG TGCGTCGCGC GGCTACATCG AGACGCACGG CGCGCCGCAG CGGCCGCAGG ATCTCGCGGG CCACGTGTGC CTCGGGATGG TCGCGCCGGG CCTGTACTGG GACGAATGGA AACTGATGGG GCCGCACGGC GAGGAGAGCG TGACGCTCGC GCCGCCGCCG TTTCGCGTGA ACGTCGCGGA AGCGCTCGCG GCCGGCATCC GCGAGGGAAT GGGCATCGGC GTTCTGCCGC TCTATTCGGC GATCGCCGGG CTGCGCCACG GCGATTTCGT GCGCGTGATG CCCGAATACC GTTCGCACGT GATGAACATC TACGCGCTCT ATGCATCGCG CCAGTATCTC GATGCGAAGA TCCGCACGTG GGTCGATTTC CTGCGCGACG AGCTGCCGTC GATCCTCGAA GCCGACGAAG CGGCGCTCGA GCGCTTCACC GTGCAGACC

Details for ButhA.12800.d.A1.GE34785

HARVESTED ON:	3/13/2012
SEQUENCED ON:	3/12/2012
EXPECTED MW:	37kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMDTLQNMRV FVRVVDAGSF TAAAQQLNTT TAYASRAVSD LEAHLRTRLL NRTTRRIALT EAGERYLQRC EQILAYVEQA EAEASDAHAR PSGKLKVHCM TSLGQHYTVP AVSRYRQRYP EVQVDLTLAQ RLPDLLDEGF DVSLVVGREL PDSGLVSQRL GETFSVVCAS RGYIETHGAP QRPQDLAGHV CLGMVAPGLY WDEWKLMGPH GEESVTLAPP PFRVNVAEAL AAGIREGMGI GVLPLYSAIA GLRHGDFVRV MPEYRSHVMN IYALYASRQY LDAKIRTWVD FLRDELPSIL EADEAALERF T

Validated NT Sequence

cacggtgaag cgctcgagcg ccgcttcgtc ggcttcgagg atcgacggca gctcgtcgcg caggaaatcg acccacgtgc ggatcttcgc atcgagatac tggcgcgatg catagagcgc gtagatgttc atcacgtgcg aacggtattc gggcatcacg cgcacgaaat cgccgtggcg cagcccggcg atcgccgaat agagcggcag aacgccgatg cccattccct cgcggatgcc ggccgcgagc gcttccgcga cgttcacgcg aaacggcggc ggcgcgagcg tcacgctctc ctcgccgtgc ggccccatca gtttccattc gtcccagtac aggcccggcg cgaccatccc gaggcacacg tggcccgcga gatcctgcgg ccgctgcggc gcgccgtgcg tctcgatgta gccgcgcgac gcacagacga cgctgaacgt ctcgccgagc cgctgcgaca cgagccccga atcgggcagt tcgcggccga cgacgagcga cacgtcgaag ccttcgtcga gaagatccgg cagccgctgc gcgagcgtca ggtcgacctg gacctccgga tagcgctgcc ggtagcgcga caccgcgggc accgtgtagt gctggccgag gctcgtcatg cagtgcacct tcagcttgcc ggacgggcgc gcatgcgcgt cgctcgcctc ggcctccgcc tgctcgacgt atgcgaggat ctgctcgcag cgctgcagat agcgctcgcc cgcctcggtg agcgcgatgc gccgcgtcgt acggttcaaa agacgcgtgc gcaggtgcgc ctcgagatcg gacaccgcgc gcgacgcgta cgcggtcgtc gtattgagct gctgcgccgc cgccgtgaag ctgcccgcat cgaccacgcg aacgaacaca cgcatgtttt ggagcgtatc catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccatggta tntctcctt

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggatacgctc caaaacatgc gtgtgttcgt tcgcgtggtc gatgcgggca gcttcacggc ggcggcgcag cagctcaata cgacgaccgc gtacgcgtcg cgcgcggtgt ccgatctcga ggcgcacctg cgcacgcgtc ttttgaaccg tacgacgcgg cgcatcgcgc tcaccgaggc gggcgagcgc tatctgcagc gctgcgagca gatcctcgca tacgtcgagc aggcggaggc cgaggcgagc gacgcgcatg cgcgcccgtc cggcaagctg aaggtgcact gcatgacgag cctcggccag cactacacgg tgcccgcggt gtcgcgctac cggcagcgct atccggaggt ccaggtcgac ctgacgctcg cgcagcggct gccggatctt ctcgacgaag gcttcgacgt gtcgctcgtc gtcggccgcg aactgcccga ttcggggctc gtgtcgcagc ggctcggcga gacgttcagc gtcgtctgtg cgtcgcgcgg ctacatcgag acgcacggcg cgccgcagcg gccgcaggat ctcgcgggcc acgtgtgcct cgggatggtc gcgccgggcc tgtactggga cgaatggaaa ctgatggggc cgcacggcga ggagagcgtg acgctcgcgc cgccgccgtt tcgcgtgaac gtcgcggaag cgctcgcggc cggcatccgc gagggaatgg gcatcggcgt tctgccgctc tattcggcga tcgccgggct gcgccacggc gatttcgtgc gcgtgatgcc cgaataccgt tcgcacgtga tgaacatcta cgcgctctat gcatcgcgcc agtatctcga tgcgaagatc cgcacgtggg tcgatttcct gcgcgacgag ctgccgtcga tcctcgaagc cgacgaagcg gcgctcgagc gcttcaccgt gcagacctaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa