ButhA.11726.a

N-acetylmuramoyl-L-alanine amidase (BTH_I0722)

CENTER ID: ButhA.11726.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.11726.a.A1.GE32771 full length 1 514
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.3430
RefSeq: YP_441278.1
UniProt: Q2T0M1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLIKPFRSIE SAATATHNWR RRQILRAGAS TLVLGLALPR LAHASSVLGV RVWPARDYTR VTIESDQPLQ NTQQLLQGPD RLVVDLNGLE LDQALRDLVS KIAPNDPQIQ SVRVGQYQPH VVRMVFDLKG SVKPQVFTLP PVGTYKYRLV FDLYPAVAPD PLSDLIAQTE RKEQQLNDTL RAQQAQPPTA ALNGPTAAPP AATDNSDAFF QRFAQNGTPP AHSPRPAPAN PPVAQTAPPA APQRPAVKPP PVVARKNDDD SGDADTYAFT APKSGRGTVR LLTVAIDPGH GGEDPGAIGG AGTYEKHIAL DIAKKLRAKI DAAPNMRAMM TRDADFFVPL NVRVQKARRV GADLFVSIHA DAFTTPSARG SSVFALSDHG ASSAAARWLA NKENSSDLIG GINIKTQDVS VNRALFDMST TAQIRDSLRY GNYVLREVGG INKLHKGSVE QAGFAVLKAP DIPSILVETA FISNPDEERR LNDDAYRDQM ADAIFRGIKR YFAANPPLAK SRMT
NT Sequence
atgttgatca aaccgttccg ctcgatcgaa tcggcggcca ccgcgaccca taactggcga cgccggcaga tcctgcgcgc cggcgcgtcg acgctcgtgc tcgggctcgc gctgccgcgc ctcgcgcacg cgagctccgt gctcggcgtg cgcgtgtggc ccgcgcgcga ctacacgcgc gtgacgatcg aatcggatca gccgctgcag aacacgcagc aactgctgca agggccggac cggctcgtcg tcgacctgaa cggcctcgag ctcgaccagg cgctgcgcga cctcgtgtcg aagatcgcgc cgaacgatcc gcagatccag tcggtgcggg tcggccagta tcagccgcat gtcgtgcgga tggtgttcga cctgaagggc tcggtgaagc cgcaggtgtt cacgctgccg cccgtcggca cctacaagta ccggctcgtg ttcgacctgt atccggccgt cgcgcccgac ccgctgtccg acctgatcgc gcagacggag cgcaaggaac agcagttgaa cgatacgctg cgcgcgcagc aggcgcagcc gccgacggcc gcgctcaacg gcccgaccgc cgcgccgccc gccgcgaccg acaacagcga tgcgttcttc cagcgcttcg cgcagaacgg cacgccgccc gcgcactcgc cgcggccggc gcccgccaat ccgcccgtcg cgcagacggc accgcccgcc gcgccgcagc ggcccgcggt caagccgccg cccgtcgtcg cgcgcaagaa cgacgacgat tccggcgacg ccgacaccta cgcgttcacc gcgccgaagt ccggccgcgg caccgtgcgc ctgctgacgg tcgcgatcga cccgggccac ggcggcgagg acccgggcgc gatcggcggc gccggcacgt acgagaagca catcgcgctc gacatcgcga agaagctgcg cgcgaagatc gacgcggcgc ccaacatgcg cgcgatgatg acgcgcgacg ccgacttctt cgtcccgctg aacgtgcgcg tgcagaaggc gcggcgcgtc ggcgcggatc tgttcgtatc gattcacgcg gatgcgttca cgacgccgtc ggcgcgcggc tcgtcggtgt tcgcgctgtc ggaccacggc gcgtcgagcg cggccgcgcg ctggctcgcg aacaaggaga actcgtcgga cctgatcggc gggatcaaca tcaagacgca ggacgtctcg gtgaaccgcg cgctcttcga catgtcgacg accgcgcaga tccgcgattc gctgcgctac ggcaattacg tgctgcgcga agtcggcggc atcaacaagc tgcacaaggg ctcggtcgag caggcggggt tcgcggtgct gaaagcgccc gacattccgt cgatcctcgt cgagaccgcg ttcatcagca atccggacga agagcggcgg ctcaacgatg acgcgtatcg cgaccagatg gccgacgcga tcttccgcgg gatcaagcgc tatttcgcgg cgaatccgcc gctcgcgaag agccggatga cgtaa
Details for ButhA.11726.a.A1.GE32771
HARVESTED ON: 6/20/2011
SEQUENCED ON: 7/14/2011
EXPECTED MW: 57kDa
OBSERVED MW: 60kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 90
PERCENT COVERAGE: 54
Validated AA Sequence
MXHHHHHHMG TLEAQTQGPG SMLIKPFRSI ESAATATHNW RRRQILRAGA STLVLGLALP RLAHASSVLG VRVWPARDYT RVTIESDQPL QNTQQLLQGP DRLVVDLNGL ELDQALRDLV SKIAPNDPQI QSVRVGQYQP HVVRMVFDLK GSVKPQVFTL PPVGTYKYRL VFDLYPAVAP DPLSDLIAQT ERKEQQLNDT LRAQXXQPPT AXXXGPTAAX XAATDNSDAF FQRFAQNGTX XAHSPRXXPA NPPVAXTXPP XAXXXXAVKP XXXVARKNXX XSGXXXXYAF T
Validated NT Sequence
atggntcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgttga tcaaaccgtt ccgctcgatc gaatcggcgg ccaccgcgac ccataactgg cgacgccggc agatcctgcg cgccggcgcg tcgacgctcg tgctcgggct cgcgctgccg cgcctcgcgc acgcgagctc cgtgctcggc gtgcgcgtgt ggcccgcgcg cgactacacg cgcgtgacga tcgaatcgga tcagccgctg cagaacacgc agcaactgct gcaagggccg gaccggctcg tcgtcgacct gaacggcctc gagctcgacc aggcgctgcg cgacctcgtg tcgaagatcg cgccgaacga tccgcagatc cagtcggtgc gggtcggcca gtatcagccg catgtcgtgc ggatggtgtt cgacctgaag ggctcggtga agccgcaggt gttcacgctg ccgcccgtcg gcacctacaa gtaccggctc gtgttcgacc tgtatccggc cgtcgcgccc gacccgctgt ccgacctgat cgcgcagacn gagcgcaagg aacagcagtt gaacgatacg ctgcgcgcgc agcannngca gccgccgacg gccgnnntcn acggcccgac cgccgcgncn nccgccgcga ccgacaacag cgatgcgttc ttccagcgct tcgcgcagaa cggcacgnnn nncgcgcact cgccgcggnc ngngcccgcc aatccgcccg tcgcgcanac nnnaccgccc gnngcgncnn anngggncgc ggtcaagccg nnnncnntcg tcgcgcgcaa gaacgannan nnttccggnn anncnnannc ctacgcnttc accg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLIKPFRSI ESAATATHNW RRRQILRAGA STLVLGLALP RLAHASSVLG VRVWPARDYT RVTIESDQPL QNTQQLLQGP DRLVVDLNGL ELDQALRDLV SKIAPNDPQI QSVRVGQYQP HVVRMVFDLK GSVKPQVFTL PPVGTYKYRL VFDLYPAVAP DPLSDLIAQT ERKEQQLNDT LRAQQAQPPT AALNGPTAAP PAATDNSDAF FQRFAQNGTP PAHSPRPAPA NPPVAQTAPP AAPQRPAVKP PPVVARKNDD DSGDADTYAF TAPKSGRGTV RLLTVAIDPG HGGEDPGAIG GAGTYEKHIA LDIAKKLRAK IDAAPNMRAM MTRDADFFVP LNVRVQKARR VGADLFVSIH ADAFTTPSAR GSSVFALSDH GASSAAARWL ANKENSSDLI GGINIKTQDV SVNRALFDMS TTAQIRDSLR YGNYVLREVG GINKLHKGSV EQAGFAVLKA PDIPSILVET AFISNPDEER RLNDDAYRDQ MADAIFRGIK RYFAANPPLA KSRMT
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgatcaaa ccgttccgct cgatcgaatc ggcggccacc gcgacccata actggcgacg ccggcagatc ctgcgcgccg gcgcgtcgac gctcgtgctc gggctcgcgc tgccgcgcct cgcgcacgcg agctccgtgc tcggcgtgcg cgtgtggccc gcgcgcgact acacgcgcgt gacgatcgaa tcggatcagc cgctgcagaa cacgcagcaa ctgctgcaag ggccggaccg gctcgtcgtc gacctgaacg gcctcgagct cgaccaggcg ctgcgcgacc tcgtgtcgaa gatcgcgccg aacgatccgc agatccagtc ggtgcgggtc ggccagtatc agccgcatgt cgtgcggatg gtgttcgacc tgaagggctc ggtgaagccg caggtgttca cgctgccgcc cgtcggcacc tacaagtacc ggctcgtgtt cgacctgtat ccggccgtcg cgcccgaccc gctgtccgac ctgatcgcgc agacggagcg caaggaacag cagttgaacg atacgctgcg cgcgcagcag gcgcagccgc cgacggccgc gctcaacggc ccgaccgccg cgccgcccgc cgcgaccgac aacagcgatg cgttcttcca gcgcttcgcg cagaacggca cgccgcccgc gcactcgccg cggccggcgc ccgccaatcc gcccgtcgcg cagacggcac cgcccgccgc gccgcagcgg cccgcggtca agccgccgcc cgtcgtcgcg cgcaagaacg acgacgattc cggcgacgcc gacacctacg cgttcaccgc gccgaagtcc ggccgcggca ccgtgcgcct gctgacggtc gcgatcgacc cgggccacgg cggcgaggac ccgggcgcga tcggcggcgc cggcacgtac gagaagcaca tcgcgctcga catcgcgaag aagctgcgcg cgaagatcga cgcggcgccc aacatgcgcg cgatgatgac gcgcgacgcc gacttcttcg tcccgctgaa cgtgcgcgtg cagaaggcgc ggcgcgtcgg cgcggatctg ttcgtatcga ttcacgcgga tgcgttcacg acgccgtcgg cgcgcggctc gtcggtgttc gcgctgtcgg accacggcgc gtcgagcgcg gccgcgcgct ggctcgcgaa caaggagaac tcgtcggacc tgatcggcgg gatcaacatc aagacgcagg acgtctcggt gaaccgcgcg ctcttcgaca tgtcgacgac cgcgcagatc cgcgattcgc tgcgctacgg caattacgtg ctgcgcgaag tcggcggcat caacaagctg cacaagggct cggtcgagca ggcggggttc gcggtgctga aagcgcccga cattccgtcg atcctcgtcg agaccgcgtt catcagcaat ccggacgaag agcggcggct caacgatgac gcgtatcgcg accagatggc cgacgcgatc ttccgcggga tcaagcgcta tttcgcggcg aatccgccgc tcgcgaagag ccggatgacg taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa