ButhA.10537.a

Cell division protein FtsA (BTH_I1122) (ftsA)

CENTER ID: ButhA.10537.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.10537.a.A1.GE32770 full length 1 410
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.10537.a.A1.PS01232 full length 1 410

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.3878
RefSeq: YP_441670.1
UniProt: Q2SZH9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSKDYKDLLV ALDIGTSKVV AVVAELKGEG HYEVIGLGQS ESKGLKKGVV VNIEATVQSI QRALEEAELM ADCKITNVFT GIAGSHIRSF NSSGMVAIKE KEVTQTDVAR VIETAKAINI PTDQQVLHIL TQEFIIDGQE DVREPIGMSG IRLEVKVHIV TGAVSAAQNI VKCVRRCGLE VNDLILQPLA SSLAVLTEDE KDLGVVLVDI GGGTTDIAIF AEGAIRHTAV IPIAGDQITS DIAMALRTPT PDAEDIKVGY GIAKQALADP DEMVEVPGLG ERGPRTLSRQ ALAAVIEPRV EELFSLVQQV VRESGYEELL SSGVVLTGGA AMMPGMVELG EDIFLKPVRI GAPEYAGGLS DVVRNPRYST AMGLLVEGSA QRMRGRKVAV QSGSAGQIFS RMKEWFLSNF
NT Sequence
atgagcaaag actacaagga tctgctggtt gccctcgaca tcggcacgtc gaaggtggtg gccgtcgtcg ccgagctgaa gggcgaaggc cactatgagg tcatcggcct cggccagagc gagtcgaagg ggctgaagaa aggcgtggtg gtgaacatcg aggccaccgt tcaatcgatt cagcgcgcgc tcgaagaagc cgagctgatg gccgactgca agatcaccaa cgtcttcacc ggcattgccg gcagccacat ccgcagcttc aactcgagcg ggatggtcgc gatcaaggag aaggaagtca cgcagaccga cgtcgcgcgc gtgatcgaaa cggcgaaggc gatcaacatc ccgaccgacc agcaggtgct gcacatcctc acgcaggaat tcatcatcga cggccaggaa gacgtgcgcg agccgatcgg catgagcggc atccgcctcg aggtgaaggt gcacatcgtg acgggcgcgg tgagcgccgc gcaaaacatc gtcaagtgcg tgcgccgctg cgggctcgag gtgaacgacc tgatcctgca gccgctcgcg tcgtcgcttg cggtgctgac ggaagacgag aaggacttgg gcgtcgtgct cgtcgacatc ggcggcggca cgacggacat cgcgatcttc gccgaaggcg cgattcgcca cacggcggtg attccgatcg cgggcgacca gatcacgagc gacatcgcga tggcgctgcg cacgccgacg cccgacgccg aagacatcaa ggtcggctac gggatcgcga agcaggcgct cgccgatccg gacgaaatgg tcgaagtgcc gggcctgggc gaacgcggcc cgcgcacgct gtcgcggcag gcgctcgcgg ccgtcatcga gccgcgcgtc gaggagctgt tctcgctcgt gcagcaggtc gtgcgcgagt ccggttacga agagttactc agttccggtg tcgtgttgac gggcggggct gcgatgatgc ccggcatggt cgagctgggc gaagacattt tcctgaagcc ggtgcgcatc ggcgcgccgg aatatgcggg cgggctctcg gacgtcgtgc gcaatccgcg ctattcgacg gcgatggggc tgctcgtcga gggcagcgct caacggatgc gcgggcgcaa ggtcgccgtg caatccggtt ccgcggggca gatcttctcg cggatgaagg agtggttcct cagcaacttc taa
Details for ButhA.10537.a.A1.GE32770
HARVESTED ON: 6/20/2011
SEQUENCED ON: 7/14/2011
EXPECTED MW: 46kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSKDYKDLL VALDIGTSKV VAVVAELKGE GHYEVIGLGQ SESKGLKKGV VVNIEATVQS IQRALEEAEL MADCKITNVF TGIAGSHIRS FNSSGMVAIK EKEVTQTDVA RVIETAKAIN IPTDQQVLHI LTQEFIIDGQ EDVREPIGMS GIRLEVKVHI VTGAVSAAQN IVKCVRRCGL EVNDLILQPL ASSLAVLTED EKDLGVVLVD IGGGTTDIAI FAEGAIRHTA VIPIAGDQIT SDIAMALRTP TPDAEDIKVG YGIAKQALAD PDEMVEVPGL GERGPRTLSR QALAAVIEPR VEELFSLVQQ VVRESGYEEL LSSGVVLTGG AAMMPGMVEL GEDIFLKPVR IGAPEYAGGL SDVVRNPRYS TAMGLLVEGS AQRMRGRKVA VQSGSAGQIF SRMKEWFLS
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagca aagactacaa ggatctgctg gttgccctcg acatcggcac gtcgaaggtg gtggccgtcg tcgccgagct gaagggcgaa ggccactatg aggtcatcgg cctcggccag agcgagtcga aggggctgaa gaaaggcgtg gtggtgaaca tcgaggccac cgttcaatcg attcagcgcg cgctcgaaga agccgagctg atggccgact gcaagatcac caacgtcttc accggcattg ccggcagcca catccgcagc ttcaactcga gcgggatggt cgcgatcaag gagaaggaag tcacgcagac cgacgtcgcg cgcgtgatcg aaacggcgaa ggcgatcaac atcccgaccg accagcaggt gctgcacatc ctcacgcagg aattcatcat cgacggccag gaagacgtgc gcgagccgat cggcatgagc ggcatccgcc tcgaggtgaa ggtgcacatc gtgacgggcg cggtgagcgc cgcgcaaaac atcgtcaagt gcgtgcgccg ctgcgggctc gaggtgaacg acctgatcct gcagccgctc gcgtcgtcgc ttgcggtgct gacggaagac gagaaggact tgggcgtcgt gctcgtcgac atcggcggcg gcacgacgga catcgcgatc ttcgccgaag gcgcgattcg ccacacggcg gtgattccga tcgcgggcga ccagatcacg agcgacatcg cgatggcgct gcgcacgccg acgcccgacg ccgaagacat caaggtcggc tacgggatcg cgaagcaggc gctcgccgat ccggacgaaa tggtcgaagt gccgggcctg ggcgaacgcg gcccgcgcac gctgtcgcgg caggcgctcg cggccgtcat cgagccgcgc gtcgaggagc tgttctcgct cgtgcagcag gtcgtgcgcg agtccggtta cgaagagtta ctcagttccg gtgtcgtgtt gacgggcggg gctgcgatga tgcccggcat ggtcgagctg ggcgaagaca ttttcctgaa gccggtgcgc atcggcgcgc cggaatatgc gggcgggctc tcggacgtcg tgcgcaatcc gcgctattcg acggcgatgg ggctgctcgt cgagggcagc gctcaacgga tgcgcgggcg caaggtcgcc gtgcaatccg gttccgcggg gcagatcttc tcgcggatga aggagtggtt cctcagca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSKDYKDLL VALDIGTSKV VAVVAELKGE GHYEVIGLGQ SESKGLKKGV VVNIEATVQS IQRALEEAEL MADCKITNVF TGIAGSHIRS FNSSGMVAIK EKEVTQTDVA RVIETAKAIN IPTDQQVLHI LTQEFIIDGQ EDVREPIGMS GIRLEVKVHI VTGAVSAAQN IVKCVRRCGL EVNDLILQPL ASSLAVLTED EKDLGVVLVD IGGGTTDIAI FAEGAIRHTA VIPIAGDQIT SDIAMALRTP TPDAEDIKVG YGIAKQALAD PDEMVEVPGL GERGPRTLSR QALAAVIEPR VEELFSLVQQ VVRESGYEEL LSSGVVLTGG AAMMPGMVEL GEDIFLKPVR IGAPEYAGGL SDVVRNPRYS TAMGLLVEGS AQRMRGRKVA VQSGSAGQIF SRMKEWFLSN F
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcaaagac tacaaggatc tgctggttgc cctcgacatc ggcacgtcga aggtggtggc cgtcgtcgcc gagctgaagg gcgaaggcca ctatgaggtc atcggcctcg gccagagcga gtcgaagggg ctgaagaaag gcgtggtggt gaacatcgag gccaccgttc aatcgattca gcgcgcgctc gaagaagccg agctgatggc cgactgcaag atcaccaacg tcttcaccgg cattgccggc agccacatcc gcagcttcaa ctcgagcggg atggtcgcga tcaaggagaa ggaagtcacg cagaccgacg tcgcgcgcgt gatcgaaacg gcgaaggcga tcaacatccc gaccgaccag caggtgctgc acatcctcac gcaggaattc atcatcgacg gccaggaaga cgtgcgcgag ccgatcggca tgagcggcat ccgcctcgag gtgaaggtgc acatcgtgac gggcgcggtg agcgccgcgc aaaacatcgt caagtgcgtg cgccgctgcg ggctcgaggt gaacgacctg atcctgcagc cgctcgcgtc gtcgcttgcg gtgctgacgg aagacgagaa ggacttgggc gtcgtgctcg tcgacatcgg cggcggcacg acggacatcg cgatcttcgc cgaaggcgcg attcgccaca cggcggtgat tccgatcgcg ggcgaccaga tcacgagcga catcgcgatg gcgctgcgca cgccgacgcc cgacgccgaa gacatcaagg tcggctacgg gatcgcgaag caggcgctcg ccgatccgga cgaaatggtc gaagtgccgg gcctgggcga acgcggcccg cgcacgctgt cgcggcaggc gctcgcggcc gtcatcgagc cgcgcgtcga ggagctgttc tcgctcgtgc agcaggtcgt gcgcgagtcc ggttacgaag agttactcag ttccggtgtc gtgttgacgg gcggggctgc gatgatgccc ggcatggtcg agctgggcga agacattttc ctgaagccgg tgcgcatcgg cgcgccggaa tatgcgggcg ggctctcgga cgtcgtgcgc aatccgcgct attcgacggc gatggggctg ctcgtcgagg gcagcgctca acggatgcgc gggcgcaagg tcgccgtgca atccggttcc gcggggcaga tcttctcgcg gatgaaggag tggttcctca gcaacttcta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for ButhA.10537.a.A1.PS01232
PURIFICATION DATe: 10/6/2011
CONCENTRATION: 31.87mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSKDYKDLL VALDIGTSKV VAVVAELKGE GHYEVIGLGQ SESKGLKKGV VVNIEATVQS IQRALEEAEL MADCKITNVF TGIAGSHIRS FNSSGMVAIK EKEVTQTDVA RVIETAKAIN IPTDQQVLHI LTQEFIIDGQ EDVREPIGMS GIRLEVKVHI VTGAVSAAQN IVKCVRRCGL EVNDLILQPL ASSLAVLTED EKDLGVVLVD IGGGTTDIAI FAEGAIRHTA VIPIAGDQIT SDIAMALRTP TPDAEDIKVG YGIAKQALAD PDEMVEVPGL GERGPRTLSR QALAAVIEPR VEELFSLVQQ VVRESGYEEL LSSGVVLTGG AAMMPGMVEL GEDIFLKPVR IGAPEYAGGL SDVVRNPRYS TAMGLLVEGS AQRMRGRKVA VQSGSAGQIF SRMKEWFLS
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagca aagactacaa ggatctgctg gttgccctcg acatcggcac gtcgaaggtg gtggccgtcg tcgccgagct gaagggcgaa ggccactatg aggtcatcgg cctcggccag agcgagtcga aggggctgaa gaaaggcgtg gtggtgaaca tcgaggccac cgttcaatcg attcagcgcg cgctcgaaga agccgagctg atggccgact gcaagatcac caacgtcttc accggcattg ccggcagcca catccgcagc ttcaactcga gcgggatggt cgcgatcaag gagaaggaag tcacgcagac cgacgtcgcg cgcgtgatcg aaacggcgaa ggcgatcaac atcccgaccg accagcaggt gctgcacatc ctcacgcagg aattcatcat cgacggccag gaagacgtgc gcgagccgat cggcatgagc ggcatccgcc tcgaggtgaa ggtgcacatc gtgacgggcg cggtgagcgc cgcgcaaaac atcgtcaagt gcgtgcgccg ctgcgggctc gaggtgaacg acctgatcct gcagccgctc gcgtcgtcgc ttgcggtgct gacggaagac gagaaggact tgggcgtcgt gctcgtcgac atcggcggcg gcacgacgga catcgcgatc ttcgccgaag gcgcgattcg ccacacggcg gtgattccga tcgcgggcga ccagatcacg agcgacatcg cgatggcgct gcgcacgccg acgcccgacg ccgaagacat caaggtcggc tacgggatcg cgaagcaggc gctcgccgat ccggacgaaa tggtcgaagt gccgggcctg ggcgaacgcg gcccgcgcac gctgtcgcgg caggcgctcg cggccgtcat cgagccgcgc gtcgaggagc tgttctcgct cgtgcagcag gtcgtgcgcg agtccggtta cgaagagtta ctcagttccg gtgtcgtgtt gacgggcggg gctgcgatga tgcccggcat ggtcgagctg ggcgaagaca ttttcctgaa gccggtgcgc atcggcgcgc cggaatatgc gggcgggctc tcggacgtcg tgcgcaatcc gcgctattcg acggcgatgg ggctgctcgt cgagggcagc gctcaacgga tgcgcgggcg caaggtcgcc gtgcaatccg gttccgcggg gcagatcttc tcgcggatga aggagtggtt cctcagca
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSKDYKDLL VALDIGTSKV VAVVAELKGE GHYEVIGLGQ SESKGLKKGV VVNIEATVQS IQRALEEAEL MADCKITNVF TGIAGSHIRS FNSSGMVAIK EKEVTQTDVA RVIETAKAIN IPTDQQVLHI LTQEFIIDGQ EDVREPIGMS GIRLEVKVHI VTGAVSAAQN IVKCVRRCGL EVNDLILQPL ASSLAVLTED EKDLGVVLVD IGGGTTDIAI FAEGAIRHTA VIPIAGDQIT SDIAMALRTP TPDAEDIKVG YGIAKQALAD PDEMVEVPGL GERGPRTLSR QALAAVIEPR VEELFSLVQQ VVRESGYEEL LSSGVVLTGG AAMMPGMVEL GEDIFLKPVR IGAPEYAGGL SDVVRNPRYS TAMGLLVEGS AQRMRGRKVA VQSGSAGQIF SRMKEWFLSN F
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCAAAGAC TACAAGGATC TGCTGGTTGC CCTCGACATC GGCACGTCGA AGGTGGTGGC CGTCGTCGCC GAGCTGAAGG GCGAAGGCCA CTATGAGGTC ATCGGCCTCG GCCAGAGCGA GTCGAAGGGG CTGAAGAAAG GCGTGGTGGT GAACATCGAG GCCACCGTTC AATCGATTCA GCGCGCGCTC GAAGAAGCCG AGCTGATGGC CGACTGCAAG ATCACCAACG TCTTCACCGG CATTGCCGGC AGCCACATCC GCAGCTTCAA CTCGAGCGGG ATGGTCGCGA TCAAGGAGAA GGAAGTCACG CAGACCGACG TCGCGCGCGT GATCGAAACG GCGAAGGCGA TCAACATCCC GACCGACCAG CAGGTGCTGC ACATCCTCAC GCAGGAATTC ATCATCGACG GCCAGGAAGA CGTGCGCGAG CCGATCGGCA TGAGCGGCAT CCGCCTCGAG GTGAAGGTGC ACATCGTGAC GGGCGCGGTG AGCGCCGCGC AAAACATCGT CAAGTGCGTG CGCCGCTGCG GGCTCGAGGT GAACGACCTG ATCCTGCAGC CGCTCGCGTC GTCGCTTGCG GTGCTGACGG AAGACGAGAA GGACTTGGGC GTCGTGCTCG TCGACATCGG CGGCGGCACG ACGGACATCG CGATCTTCGC CGAAGGCGCG ATTCGCCACA CGGCGGTGAT TCCGATCGCG GGCGACCAGA TCACGAGCGA CATCGCGATG GCGCTGCGCA CGCCGACGCC CGACGCCGAA GACATCAAGG TCGGCTACGG GATCGCGAAG CAGGCGCTCG CCGATCCGGA CGAAATGGTC GAAGTGCCGG GCCTGGGCGA ACGCGGCCCG CGCACGCTGT CGCGGCAGGC GCTCGCGGCC GTCATCGAGC CGCGCGTCGA GGAGCTGTTC TCGCTCGTGC AGCAGGTCGT GCGCGAGTCC GGTTACGAAG AGTTACTCAG TTCCGGTGTC GTGTTGACGG GCGGGGCTGC GATGATGCCC GGCATGGTCG AGCTGGGCGA AGACATTTTC CTGAAGCCGG TGCGCATCGG CGCGCCGGAA TATGCGGGCG GGCTCTCGGA CGTCGTGCGC AATCCGCGCT ATTCGACGGC GATGGGGCTG CTCGTCGAGG GCAGCGCTCA ACGGATGCGC GGGCGCAAGG TCGCCGTGCA ATCCGGTTCC GCGGGGCAGA TCTTCTCGCG GATGAAGGAG TGGTTCCTCA GCAACTTCTA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A