ButhA.10478.b

Branched-chain amino acid aminotransferase

CENTER ID: ButhA.10478.b
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.10478.b.B1.GE36210 Full length( ButhA.10478.b ) 1 314
ButhA.10478.b.B1.GE36210 Full length( ButhA.10478.b ) 1 314
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.10478.b.B1.PS01801 Full length( ButhA.10478.b ) 1 314

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.649
RefSeq: YP_438767.1
UniProt: Q2T7T2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKASYVWKNG ELVPWERAQV HVMSHALHYG TSVFEGVRAY EIGEKAAILC GREHFERLLF SCKVARIPSP MSVGQWMEAT VETLRANGQR SAYIRPLVYR GAGESLGLDA RQCPAEALLI TTPWGAYLGD EALQQGVDVQ VSSWRRNGGG AASTLAKIGG QYVNGQAIVM EAHENGMSEG IALDANGFVS EGSGENVFLV YKNEIFTPSI GSSILSGITR NCVIRIARDL GYTVTETSIP REMLYLADEI FFTGTAVEIC PVRSVDRMPV GSGKRGAVTK AIQDQYFGIV RGTHADKWNW LTPVPVPSRD GARA
NT Sequence
ATGAAAGCGA GCTACGTGTG GAAGAACGGC GAACTGGTGC CGTGGGAGCG CGCACAGGTG CATGTGATGA GCCATGCGCT GCATTACGGG ACGAGCGTGT TCGAGGGCGT GCGTGCGTAC GAGATCGGCG AGAAGGCGGC GATCCTGTGC GGCCGCGAGC ACTTCGAGCG CCTGCTGTTC AGCTGCAAGG TCGCGCGGAT TCCGTCGCCG ATGAGCGTCG GGCAGTGGAT GGAAGCGACG GTCGAGACGC TGCGCGCGAA CGGCCAGCGC AGCGCGTACA TCCGGCCGCT CGTCTATCGC GGCGCGGGCG AATCGCTCGG GCTCGACGCG CGCCAATGCC CGGCCGAGGC GCTGCTCATC ACCACGCCAT GGGGCGCGTA TCTCGGCGAC GAGGCGCTGC AGCAGGGCGT CGACGTGCAG GTCAGCAGCT GGCGCCGCAA CGGCGGCGGG GCGGCGAGCA CGCTCGCGAA GATCGGCGGC CAGTACGTGA ACGGCCAGGC GATCGTGATG GAGGCGCATG AGAACGGGAT GAGCGAAGGC ATCGCGCTCG ATGCGAACGG TTTCGTCAGC GAAGGCAGCG GCGAAAACGT GTTTCTCGTC TACAAGAACG AGATCTTCAC GCCGTCGATC GGCAGCAGCA TCCTGAGCGG CATCACGCGC AACTGCGTGA TTCGGATCGC GCGCGACCTC GGCTACACCG TCACCGAAAC GAGCATTCCG CGCGAAATGC TCTATCTCGC CGACGAAATC TTCTTCACGG GCACGGCGGT CGAGATCTGC CCGGTGCGCT CGGTCGATCG GATGCCGGTC GGCAGCGGCA AGCGCGGCGC CGTGACGAAG GCGATCCAGG ATCAGTACTT CGGCATCGTG CGCGGCACGC ACGCGGACAA ATGGAACTGG CTCACGCCCG TGCCGGTGCC GTCGCGCGAC GGAGCACGGG CA
Details for ButhA.10478.b.B1.GE36210
HARVESTED ON: 1/23/2013
SEQUENCED ON: 1/23/2013
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHHM KASYVWKNGE LVPWERAQVH VMSHALHYGT SVFEGVRAYE IGEKAAILCG REHFERLLFS CKVARIPSPM SVGQWMEATV ETLRANGQRS AYIRPLVYRG AGESLGLDAR QCPAEALLIT TPWGAYLGDE ALQQGVDVQV SSWRRNGGGA ASTLAKIGGQ YVNGQAIVME AHENGMSEGI ALDANGFVSE GSGENVFLVY KNEIFTPSIG SSILSGITRN CVIRIARDLG YTVTETSIPR EMLYLADEIF FTGTAVEICP VRSVDRMPVG SGKRGAVTKA IQDQYFGIVR GTHADKWNWL TPVPVPSRDG ARA
Validated NT Sequence
tcttactcac ttatgcccgt gctccgtcgc gcgacggcac cggcacgggc gtgagccagt tccatttgtc cgcgtgcgtg ccgcgcacga tgccgaagta ctgatcctgg atcgccttcg tcacggcgcc gcgcttgccg ctgccgaccg gcatccgatc gaccgagcgc accgggcaga tctcgaccgc cgtgcccgtg aagaagattt cgtcggcgag atagagcatt tcgcgcggaa tgctcgtttc ggtgacggtg tagccgaggt cgcgcgcgat ccgaatcacg cagttgcgcg tgatgccgct caggatgctg ctgccgatcg acggcgtgaa gatctcgttc ttgtagacga gaaacacgtt ttcgccgctg ccttcgctga cgaaaccgtt cgcatcgagc gcgatgcctt cgctcatccc gttctcatgc gcctccatca cgatcgcctg gccgttcacg tactggccgc cgatcttcgc gagcgtgctc gccgccccgc cgccgttgcg gcgccagctg ctgacctgca cgtcgacgcc ctgctgcagc gcctcgtcgc cgagatacgc gccccatggc gtggtgatga gcagcgcctc ggccgggcat tggcgcgcgt cgagcccgag cgattcgccc gcgccgcgat agacgagcgg ccggatgtac gcgctgcgct ggccgttcgc gcgcagcgtc tcgaccgtcg cttccatcca ctgcccgacg ctcatcggcg acggaatccg cgcgaccttg cagctgaaca gcaggcgctc gaagtgctcg cggccgcaca ggatcgccgc cttctcgccg atctcgtacg cacgcacgcc ctcgaacacg ctcgtcccgt aatgcagcgc atggctcatc acatgcacct gtgcgcgctc ccacggcacc agttcgccgt tcttccacac gtagctcgct ttcatatggt ggtggtggtg gtggtgagcc atggtatntc tccttcttaa
Expected Protein Sequence
MAHHHHHHMK ASYVWKNGEL VPWERAQVHV MSHALHYGTS VFEGVRAYEI GEKAAILCGR EHFERLLFSC KVARIPSPMS VGQWMEATVE TLRANGQRSA YIRPLVYRGA GESLGLDARQ CPAEALLITT PWGAYLGDEA LQQGVDVQVS SWRRNGGGAA STLAKIGGQY VNGQAIVMEA HENGMSEGIA LDANGFVSEG SGENVFLVYK NEIFTPSIGS SILSGITRNC VIRIARDLGY TVTETSIPRE MLYLADEIFF TGTAVEICPV RSVDRMPVGS GKRGAVTKAI QDQYFGIVRG THADKWNWLT PVPVPSRDGA RA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaag cgagctacgt gtggaagaac ggcgaactgg tgccgtggga gcgcgcacag gtgcatgtga tgagccatgc gctgcattac gggacgagcg tgttcgaggg cgtgcgtgcg tacgagatcg gcgagaaggc ggcgatcctg tgcggccgcg agcacttcga gcgcctgctg ttcagctgca aggtcgcgcg gattccgtcg ccgatgagcg tcgggcagtg gatggaagcg acggtcgaga cgctgcgcgc gaacggccag cgcagcgcgt acatccggcc gctcgtctat cgcggcgcgg gcgaatcgct cgggctcgac gcgcgccaat gcccggccga ggcgctgctc atcaccacgc catggggcgc gtatctcggc gacgaggcgc tgcagcaggg cgtcgacgtg caggtcagca gctggcgccg caacggcggc ggggcggcga gcacgctcgc gaagatcggc ggccagtacg tgaacggcca ggcgatcgtg atggaggcgc atgagaacgg gatgagcgaa ggcatcgcgc tcgatgcgaa cggtttcgtc agcgaaggca gcggcgaaaa cgtgtttctc gtctacaaga acgagatctt cacgccgtcg atcggcagca gcatcctgag cggcatcacg cgcaactgcg tgattcggat cgcgcgcgac ctcggctaca ccgtcaccga aacgagcatt ccgcgcgaaa tgctctatct cgccgacgaa atcttcttca cgggcacggc ggtcgagatc tgcccggtgc gctcggtcga tcggatgccg gtcggcagcg gcaagcgcgg cgccgtgacg aaggcgatcc aggatcagta cttcggcatc gtgcgcggca cgcacgcgga caaatggaac tggctcacgc ccgtgccggt gccgtcgcgc gacggagcac gggcatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for ButhA.10478.b.B1.GE36210
HARVESTED ON: 1/23/2013
SEQUENCED ON: 1/23/2013
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHHM KASYVWKNGE LVPWERAQVH VMSHALHYGT SVFEGVRAYE IGEKAAILCG REHFERLLFS CKVARIPSPM SVGQWMEATV ETLRANGQRS AYIRPLVYRG AGESLGLDAR QCPAEALLIT TPWGAYLGDE ALQQGVDVQV SSWRRNGGGA ASTLAKIGGQ YVNGQAIVME AHENGMSEGI ALDANGFVSE GSGENVFLVY KNEIFTPSIG SSILSGITRN CVIRIARDLG YTVTETSIPR EMLYLADEIF FTGTAVEICP VRSVDRMPVG SGKRGAVTKA IQDQYFGIVR GTHADKWNWL TPVPVPSRDG ARA
Validated NT Sequence
tcttactcac ttatgcccgt gctccgtcgc gcgacggcac cggcacgggc gtgagccagt tccatttgtc cgcgtgcgtg ccgcgcacga tgccgaagta ctgatcctgg atcgccttcg tcacggcgcc gcgcttgccg ctgccgaccg gcatccgatc gaccgagcgc accgggcaga tctcgaccgc cgtgcccgtg aagaagattt cgtcggcgag atagagcatt tcgcgcggaa tgctcgtttc ggtgacggtg tagccgaggt cgcgcgcgat ccgaatcacg cagttgcgcg tgatgccgct caggatgctg ctgccgatcg acggcgtgaa gatctcgttc ttgtagacga gaaacacgtt ttcgccgctg ccttcgctga cgaaaccgtt cgcatcgagc gcgatgcctt cgctcatccc gttctcatgc gcctccatca cgatcgcctg gccgttcacg tactggccgc cgatcttcgc gagcgtgctc gccgccccgc cgccgttgcg gcgccagctg ctgacctgca cgtcgacgcc ctgctgcagc gcctcgtcgc cgagatacgc gccccatggc gtggtgatga gcagcgcctc ggccgggcat tggcgcgcgt cgagcccgag cgattcgccc gcgccgcgat agacgagcgg ccggatgtac gcgctgcgct ggccgttcgc gcgcagcgtc tcgaccgtcg cttccatcca ctgcccgacg ctcatcggcg acggaatccg cgcgaccttg cagctgaaca gcaggcgctc gaagtgctcg cggccgcaca ggatcgccgc cttctcgccg atctcgtacg cacgcacgcc ctcgaacacg ctcgtcccgt aatgcagcgc atggctcatc acatgcacct gtgcgcgctc ccacggcacc agttcgccgt tcttccacac gtagctcgct ttcatatggt ggtggtggtg gtggtgagcc atggtatntc tccttcttaa
Expected Protein Sequence
MAHHHHHHMK ASYVWKNGEL VPWERAQVHV MSHALHYGTS VFEGVRAYEI GEKAAILCGR EHFERLLFSC KVARIPSPMS VGQWMEATVE TLRANGQRSA YIRPLVYRGA GESLGLDARQ CPAEALLITT PWGAYLGDEA LQQGVDVQVS SWRRNGGGAA STLAKIGGQY VNGQAIVMEA HENGMSEGIA LDANGFVSEG SGENVFLVYK NEIFTPSIGS SILSGITRNC VIRIARDLGY TVTETSIPRE MLYLADEIFF TGTAVEICPV RSVDRMPVGS GKRGAVTKAI QDQYFGIVRG THADKWNWLT PVPVPSRDGA RA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaag cgagctacgt gtggaagaac ggcgaactgg tgccgtggga gcgcgcacag gtgcatgtga tgagccatgc gctgcattac gggacgagcg tgttcgaggg cgtgcgtgcg tacgagatcg gcgagaaggc ggcgatcctg tgcggccgcg agcacttcga gcgcctgctg ttcagctgca aggtcgcgcg gattccgtcg ccgatgagcg tcgggcagtg gatggaagcg acggtcgaga cgctgcgcgc gaacggccag cgcagcgcgt acatccggcc gctcgtctat cgcggcgcgg gcgaatcgct cgggctcgac gcgcgccaat gcccggccga ggcgctgctc atcaccacgc catggggcgc gtatctcggc gacgaggcgc tgcagcaggg cgtcgacgtg caggtcagca gctggcgccg caacggcggc ggggcggcga gcacgctcgc gaagatcggc ggccagtacg tgaacggcca ggcgatcgtg atggaggcgc atgagaacgg gatgagcgaa ggcatcgcgc tcgatgcgaa cggtttcgtc agcgaaggca gcggcgaaaa cgtgtttctc gtctacaaga acgagatctt cacgccgtcg atcggcagca gcatcctgag cggcatcacg cgcaactgcg tgattcggat cgcgcgcgac ctcggctaca ccgtcaccga aacgagcatt ccgcgcgaaa tgctctatct cgccgacgaa atcttcttca cgggcacggc ggtcgagatc tgcccggtgc gctcggtcga tcggatgccg gtcggcagcg gcaagcgcgg cgccgtgacg aaggcgatcc aggatcagta cttcggcatc gtgcgcggca cgcacgcgga caaatggaac tggctcacgc ccgtgccggt gccgtcgcgc gacggagcac gggcatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for ButhA.10478.b.B1.PS01801
PURIFICATION DATe: 4/16/2013
CONCENTRATION: 43mg/ml
OBSERVED MW: 34kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: 110µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHHM KASYVWKNGE LVPWERAQVH VMSHALHYGT SVFEGVRAYE IGEKAAILCG REHFERLLFS CKVARIPSPM SVGQWMEATV ETLRANGQRS AYIRPLVYRG AGESLGLDAR QCPAEALLIT TPWGAYLGDE ALQQGVDVQV SSWRRNGGGA ASTLAKIGGQ YVNGQAIVME AHENGMSEGI ALDANGFVSE GSGENVFLVY KNEIFTPSIG SSILSGITRN CVIRIARDLG YTVTETSIPR EMLYLADEIF FTGTAVEICP VRSVDRMPVG SGKRGAVTKA IQDQYFGIVR GTHADKWNWL TPVPVPSRDG ARA
Validated NT Sequence
tcttactcac ttatgcccgt gctccgtcgc gcgacggcac cggcacgggc gtgagccagt tccatttgtc cgcgtgcgtg ccgcgcacga tgccgaagta ctgatcctgg atcgccttcg tcacggcgcc gcgcttgccg ctgccgaccg gcatccgatc gaccgagcgc accgggcaga tctcgaccgc cgtgcccgtg aagaagattt cgtcggcgag atagagcatt tcgcgcggaa tgctcgtttc ggtgacggtg tagccgaggt cgcgcgcgat ccgaatcacg cagttgcgcg tgatgccgct caggatgctg ctgccgatcg acggcgtgaa gatctcgttc ttgtagacga gaaacacgtt ttcgccgctg ccttcgctga cgaaaccgtt cgcatcgagc gcgatgcctt cgctcatccc gttctcatgc gcctccatca cgatcgcctg gccgttcacg tactggccgc cgatcttcgc gagcgtgctc gccgccccgc cgccgttgcg gcgccagctg ctgacctgca cgtcgacgcc ctgctgcagc gcctcgtcgc cgagatacgc gccccatggc gtggtgatga gcagcgcctc ggccgggcat tggcgcgcgt cgagcccgag cgattcgccc gcgccgcgat agacgagcgg ccggatgtac gcgctgcgct ggccgttcgc gcgcagcgtc tcgaccgtcg cttccatcca ctgcccgacg ctcatcggcg acggaatccg cgcgaccttg cagctgaaca gcaggcgctc gaagtgctcg cggccgcaca ggatcgccgc cttctcgccg atctcgtacg cacgcacgcc ctcgaacacg ctcgtcccgt aatgcagcgc atggctcatc acatgcacct gtgcgcgctc ccacggcacc agttcgccgt tcttccacac gtagctcgct ttcatatggt ggtggtggtg gtggtgagcc atggtatntc tccttcttaa
Expressed Protein Sequence
MAHHHHHHMK ASYVWKNGEL VPWERAQVHV MSHALHYGTS VFEGVRAYEI GEKAAILCGR EHFERLLFSC KVARIPSPMS VGQWMEATVE TLRANGQRSA YIRPLVYRGA GESLGLDARQ CPAEALLITT PWGAYLGDEA LQQGVDVQVS SWRRNGGGAA STLAKIGGQY VNGQAIVMEA HENGMSEGIA LDANGFVSEG SGENVFLVYK NEIFTPSIGS SILSGITRNC VIRIARDLGY TVTETSIPRE MLYLADEIFF TGTAVEICPV RSVDRMPVGS GKRGAVTKAI QDQYFGIVRG THADKWNWLT PVPVPSRDGA RA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAAG CGAGCTACGT GTGGAAGAAC GGCGAACTGG TGCCGTGGGA GCGCGCACAG GTGCATGTGA TGAGCCATGC GCTGCATTAC GGGACGAGCG TGTTCGAGGG CGTGCGTGCG TACGAGATCG GCGAGAAGGC GGCGATCCTG TGCGGCCGCG AGCACTTCGA GCGCCTGCTG TTCAGCTGCA AGGTCGCGCG GATTCCGTCG CCGATGAGCG TCGGGCAGTG GATGGAAGCG ACGGTCGAGA CGCTGCGCGC GAACGGCCAG CGCAGCGCGT ACATCCGGCC GCTCGTCTAT CGCGGCGCGG GCGAATCGCT CGGGCTCGAC GCGCGCCAAT GCCCGGCCGA GGCGCTGCTC ATCACCACGC CATGGGGCGC GTATCTCGGC GACGAGGCGC TGCAGCAGGG CGTCGACGTG CAGGTCAGCA GCTGGCGCCG CAACGGCGGC GGGGCGGCGA GCACGCTCGC GAAGATCGGC GGCCAGTACG TGAACGGCCA GGCGATCGTG ATGGAGGCGC ATGAGAACGG GATGAGCGAA GGCATCGCGC TCGATGCGAA CGGTTTCGTC AGCGAAGGCA GCGGCGAAAA CGTGTTTCTC GTCTACAAGA ACGAGATCTT CACGCCGTCG ATCGGCAGCA GCATCCTGAG CGGCATCACG CGCAACTGCG TGATTCGGAT CGCGCGCGAC CTCGGCTACA CCGTCACCGA AACGAGCATT CCGCGCGAAA TGCTCTATCT CGCCGACGAA ATCTTCTTCA CGGGCACGGC GGTCGAGATC TGCCCGGTGC GCTCGGTCGA TCGGATGCCG GTCGGCAGCG GCAAGCGCGG CGCCGTGACG AAGGCGATCC AGGATCAGTA CTTCGGCATC GTGCGCGGCA CGCACGCGGA CAAATGGAAC TGGCTCACGC CCGTGCCGGT GCCGTCGCGC GACGGAGCAC GGGCATGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT