ButhA.10154.a

Histone deacetylase family protein

CENTER ID: ButhA.10154.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.10154.a.B1.GE36207 Full length( ButhA.10154.a ) 1 340
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.4921
RefSeq: YP_442634.1
UniProt: Q2SWR5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQPPSSAGYA ESPDSKRRLL SLVYASGLAA RLDMSSAPAA TDDDLLRIHP AHYLEAFKRA SDTGGGDLGE LAPFGRGSYE IAALSAGLAL AAVDAVLAER AANAFSLSRP PGHHCLRDKP MGFCLLANIP IAIEAARAKH RVERVAVIDW DVHHGNGTQA IYYDDADTLT ISLHQDRCFP PGYSGADERG AGAGIGANIN VPLLAGAGDD AYRYAFERIV LPALDAFRPE LVVVASGLDA NAVDPLARMQ LHSDSYRFMT HALKAAAQRH CAGRLVVVHE GGYSEAYVPF CGQAIVEALS GMRTDVVDPM LELAIAQQPG ERFNAFQRQL IDEMATGFGY
NT Sequence
GTGCAGCCGC CGTCGAGCGC GGGCTACGCG GAATCGCCCG ACTCGAAACG ACGCCTGCTG TCGCTCGTGT ACGCATCGGG GCTCGCCGCG AGGCTCGACA TGTCGAGCGC GCCCGCCGCG ACCGACGACG ATCTGCTGCG CATCCATCCC GCGCATTACC TCGAAGCGTT CAAGCGCGCA AGCGACACGG GCGGCGGCGA CCTCGGCGAA CTCGCGCCGT TCGGCCGGGG CAGCTATGAG ATCGCGGCGC TGTCGGCGGG GCTCGCGCTG GCCGCCGTCG ACGCGGTGCT CGCCGAGCGC GCAGCGAACG CGTTCTCGCT GTCGCGCCCG CCCGGCCATC ACTGCCTGCG CGACAAGCCG ATGGGCTTTT GCCTGCTCGC GAACATTCCG ATCGCGATCG AGGCCGCGCG CGCGAAGCAT CGCGTCGAGC GCGTCGCGGT GATCGACTGG GACGTGCATC ACGGCAACGG CACGCAGGCG ATCTACTACG ACGACGCCGA CACGCTGACG ATCTCGCTGC ATCAGGACCG CTGCTTTCCG CCCGGCTACA GCGGCGCCGA CGAGCGCGGC GCGGGCGCGG GCATCGGTGC GAACATCAAC GTTCCGCTCC TCGCGGGCGC GGGCGACGAT GCATATCGAT ATGCATTCGA GCGGATCGTG CTGCCCGCGC TCGACGCGTT CAGGCCGGAG CTCGTCGTCG TCGCGAGCGG ACTCGACGCG AATGCGGTCG ACCCGCTCGC GCGGATGCAA CTGCACAGCG ACAGCTATCG ATTCATGACG CACGCGCTGA AAGCAGCCGC GCAGCGGCAC TGCGCGGGGC GGCTCGTCGT CGTGCACGAA GGCGGTTATT CGGAGGCCTA CGTACCGTTT TGCGGACAGG CGATCGTCGA GGCGCTGTCC GGCATGCGCA CCGACGTCGT CGACCCGATG CTCGAACTCG CGATCGCGCA GCAGCCCGGC GAGCGATTCA ACGCGTTCCA GCGGCAGTTG ATCGACGAAA TGGCGACGGG CTTCGGCTAT
Details for ButhA.10154.a.B1.GE36207
HARVESTED ON: 1/23/2013
SEQUENCED ON: 1/23/2013
EXPECTED MW: 37kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 26
PERCENT COVERAGE: 68
Validated AA Sequence
MAHHHHHHMV QPPSSAGYAE SPDSKRRLLS LXXXSGXATR XXMXSAPAAT XXXXXXSXXX IXXXRXXXXX XXAXSXXXXR EAXXXXXXXR XDXXXXXAXX XPXXXXRXXX XFXXXXXAXX GRXGXXXXXX RXXXXXAXXX XXGRXRXXEG XRXXXGXXXR XXQMRXXXXR XXTXXXXXAX RPXERXXVFQ RXGVIXRXXX XXXXXXSCXX XXXXXXXXPD AXTRSXXXXA SDSTXSSXS
Validated NT Sequence
tannnntttt ntnnnnttta agaaggagan ataccatggc tcaccaccac caccaccata tggtgcagcc gccgtcgagc gcgggctacg cggaatcgcc cgactcgaaa cgacgcctgc tgtcgctcgn gtncncatcg gggntcgcna cgaggntnnn catgtnnagc gcgcccgccg cgaccganan natnngntgn nnatcnnnnn nnngcattnn nnnnnngcgt tgnnnacnnn nnnnnnnngn ngcganntcg gnnaanngnc nncgcgaggc nnnnnnnnng anngnnncnn tncggcnnga cngctgnnnn cngnaggcgn nnnnnncgcc ngngnncncg gngcggnngn gnnnnnnntt cgnannntnn nnnnntgcan nnngngggcg nnngggncnn nnnntannna nnngacgnnc tcnngnnncg ngcgcngngg ntngtcnnnn tggacgtagn cgancgtnng agggncnacg anntgnntnc ggganncnng ntcganngan gcagatgcga angnacngct ancgattnnn gacngannnn ntngnaancg cgcancggcc ctgngagcgn nnnntcgtct tccagcgcna aggagtnatt nnnaggnnnn ngtncngnnn tnnnnacngn nnatcatgcn nnntncngnn ngnnnncnna nganntcnac ccgatgctnn aactcgatcn nncnnnnnnn cngcnagcga ttcaacgcnt tcnagcngna gttgatcgan naaatgnnna cnggnttnnn ntantnannn nntaagatan nangctgcta acnannncgg anaganngan ttaanngagn nagatannnt ncnngnnnnt aanananctc cnanaggann tncnnnnnnc nnngtgnana nnntngnnnn ncgnntnnnn cnnnnnnnan nnnnnnannt tnntcntncn ntncngnnnt nntcnnnnnn ncnttnncnn nnnnnncncn nnnnngnnnn nnnnnnnnnn nnnntnnnnt nnnccnnnnn nnnnnnnnnn nncnnnnnnn nnnnnnnntn nnntannncn nnnntnnnnn nnnncnnnnn nnncnnnnnn cnnnntnnnn nnnncnnnnn nnnnnnnntn nnnnnnnnnn nc
Expected Protein Sequence
MAHHHHHHMV QPPSSAGYAE SPDSKRRLLS LVYASGLAAR LDMSSAPAAT DDDLLRIHPA HYLEAFKRAS DTGGGDLGEL APFGRGSYEI AALSAGLALA AVDAVLAERA ANAFSLSRPP GHHCLRDKPM GFCLLANIPI AIEAARAKHR VERVAVIDWD VHHGNGTQAI YYDDADTLTI SLHQDRCFPP GYSGADERGA GAGIGANINV PLLAGAGDDA YRYAFERIVL PALDAFRPEL VVVASGLDAN AVDPLARMQL HSDSYRFMTH ALKAAAQRHC AGRLVVVHEG GYSEAYVPFC GQAIVEALSG MRTDVVDPML ELAIAQQPGE RFNAFQRQLI DEMATGFGY
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtgc agccgccgtc gagcgcgggc tacgcggaat cgcccgactc gaaacgacgc ctgctgtcgc tcgtgtacgc atcggggctc gccgcgaggc tcgacatgtc gagcgcgccc gccgcgaccg acgacgatct gctgcgcatc catcccgcgc attacctcga agcgttcaag cgcgcaagcg acacgggcgg cggcgacctc ggcgaactcg cgccgttcgg ccggggcagc tatgagatcg cggcgctgtc ggcggggctc gcgctggccg ccgtcgacgc ggtgctcgcc gagcgcgcag cgaacgcgtt ctcgctgtcg cgcccgcccg gccatcactg cctgcgcgac aagccgatgg gcttttgcct gctcgcgaac attccgatcg cgatcgaggc cgcgcgcgcg aagcatcgcg tcgagcgcgt cgcggtgatc gactgggacg tgcatcacgg caacggcacg caggcgatct actacgacga cgccgacacg ctgacgatct cgctgcatca ggaccgctgc tttccgcccg gctacagcgg cgccgacgag cgcggcgcgg gcgcgggcat cggtgcgaac atcaacgttc cgctcctcgc gggcgcgggc gacgatgcat atcgatatgc attcgagcgg atcgtgctgc ccgcgctcga cgcgttcagg ccggagctcg tcgtcgtcgc gagcggactc gacgcgaatg cggtcgaccc gctcgcgcgg atgcaactgc acagcgacag ctatcgattc atgacgcacg cgctgaaagc agccgcgcag cggcactgcg cggggcggct cgtcgtcgtg cacgaaggcg gttattcgga ggcctacgta ccgttttgcg gacaggcgat cgtcgaggcg ctgtccggca tgcgcaccga cgtcgtcgac ccgatgctcg aactcgcgat cgcgcagcag cccggcgagc gattcaacgc gttccagcgg cagttgatcg acgaaatggc gacgggcttc ggctattgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t