SSGCID: Target Page

ButhA.10100.c

Serine protease

CENTER ID:	ButhA.10100.c
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.10100.c.B2.GE36206	Mature protein( ButhA.10100.c )		30	495

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.10100.c.B2.PS01831	Mature protein( ButhA.10100.c )		30	495

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.3375
RefSeq:	YP_441231.1
UniProt:	Q2T0R8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTTRILARGA VAVAVAAALS AGYVAGTRHA EPQIITPAVA ALMPAEAAAK TGIPDFSGLV ETYGPAVVNI SAKHVVQRVA QRRAAPQLPI DPEDPFYQFF RHFYGQVPGM GGGRQPQPDD QPSTSLGSGF IISADGYILT NAHVIDGANV VTVKLTDKRE YKAKVVGTDK QSDVAVLKID ASGLPTVKIG DPAQSKVGQW VVAIGSPYGF DNTVTSGIIS AKSRALPDEN YTPFIQTDVP VNPGNSGGPL FNLNGEVIGI NSMIYSQTGG FQGLSFAIPI NEAMKVKDEL VKTGHVSRGR LGVAVQGLNQ TLASSFGLQK PDGALVSSVD PKGPAAKAGL QPGDVILAVD GVPVQDSTTL PAQIASMKPG TKADLQIWRD KSKKTVSVTL ASLADDQAKA GADEPVEQGR LGVAVRPLLP RERNGTSLTH GLVVQQSTGP AASAGIQPGD VILAVNGRPV TSAEQLRDAV KRAGNSLALL IQRDDAQIFV PVDLG

NT Sequence

ATGACTACCC GAATCCTTGC GCGTGGCGCA GTTGCCGTGG CCGTCGCCGC GGCGTTGTCG GCGGGGTATG TGGCGGGCAC CCGCCATGCG GAACCGCAGA TCATCACACC GGCCGTCGCC GCGCTGATGC CGGCCGAGGC AGCCGCGAAG ACGGGCATCC CCGACTTTTC CGGCCTGGTC GAGACCTACG GGCCGGCCGT CGTGAACATC AGCGCGAAGC ACGTCGTGCA GCGCGTCGCG CAGCGCCGCG CAGCGCCGCA ATTGCCGATC GATCCGGAAG ATCCGTTCTA CCAATTCTTC CGACATTTCT ACGGGCAGGT TCCCGGGATG GGCGGTGGCC GCCAGCCGCA GCCGGACGAC CAGCCGAGCA CGAGCCTGGG CTCCGGGTTC ATCATCAGCG CGGACGGGTA TATCCTGACC AACGCGCACG TGATCGACGG CGCGAACGTC GTCACGGTGA AGCTCACCGA CAAGCGCGAG TACAAGGCGA AGGTCGTCGG CACCGACAAG CAATCCGACG TCGCGGTGCT GAAGATTGAC GCGTCGGGCC TGCCGACCGT GAAGATCGGC GATCCGGCGC AGAGCAAGGT CGGCCAGTGG GTCGTCGCGA TCGGTTCGCC GTACGGGTTC GACAACACGG TCACGTCGGG CATCATCAGC GCGAAGTCGC GCGCGCTGCC CGACGAGAAC TACACGCCGT TCATCCAGAC CGACGTGCCG GTGAACCCCG GCAACTCGGG CGGTCCGCTC TTCAACCTGA ACGGCGAGGT GATCGGCATC AACTCGATGA TCTACTCGCA GACGGGCGGC TTCCAGGGCC TGTCGTTCGC GATCCCGATC AACGAGGCGA TGAAGGTGAA GGACGAGCTC GTGAAGACGG GCCACGTGAG CCGCGGCCGG CTCGGCGTCG CGGTGCAGGG GCTCAACCAG ACGCTCGCGA GCTCGTTCGG CCTGCAAAAG CCCGACGGCG CGCTCGTCAG CTCGGTCGAC CCGAAGGGGC CGGCCGCGAA GGCGGGGCTG CAGCCGGGCG ACGTGATCCT CGCGGTCGAC GGCGTGCCGG TTCAGGACTC GACGACGTTG CCCGCGCAGA TCGCGAGCAT GAAGCCGGGC ACGAAGGCCG ACCTGCAGAT CTGGCGAGAC AAGTCGAAGA AGACAGTGTC GGTGACGCTT GCGTCGCTGG CCGACGATCA GGCGAAGGCG GGCGCCGACG AGCCCGTCGA GCAGGGACGC CTGGGCGTCG CGGTGCGGCC GCTTTTGCCG CGCGAGCGCA ACGGCACGTC GCTCACGCAC GGCCTCGTCG TCCAGCAGTC GACGGGCCCC GCCGCAAGCG CGGGCATCCA GCCGGGCGAC GTGATCCTCG CGGTGAACGG GCGGCCCGTC ACGAGCGCCG AACAATTGCG CGACGCGGTC AAGCGCGCGG GCAACAGCCT TGCGCTGCTG ATCCAGCGCG ACGACGCCCA GATTTTCGTG CCGGTCGATC TGGGC

Details for ButhA.10100.c.B2.GE36206

HARVESTED ON:	1/23/2013
SEQUENCED ON:	1/23/2013
EXPECTED MW:	50kDa
OBSERVED MW:	49kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	93

Validated AA Sequence

MPAEAAAKTG IPDFSGLVET YGPAVVNISA KHVVQRVAQR RAAPQLPIDP EDPFYQFFRH FYGQVPGMGG GRQPQPDDQP STSLGSGFII SADGYILTNA HVIDGANVVT VKLTDKREYK AKVVGTDKQS DVAVLKIDAS GLPTVKIGDP AQSKVGQWVV AIGSPYGFDN TVTSGIISAK SRALPDENYT PFIQTDVPVN PGNSGGPLFN XNGEVIGINS MIYSQTGGFQ GLSFAIPINE AMKVKDELVK TGHVSRGRLG VAVQGLNQTL ASSFGLQKPD GALVSSVDPK GPAAKAGLQP GDVILAVDGV PVQDSTTLPA QIASMKPGTK ADLQIWRDKS KKTVSVTLAS LADDQAKAGA DEPVEQGRLG VAVRPLLPRE RNGTSLTHGL VVQQSTGPAA SAGIQPGDVI LAVNGRPVTS AEQLRDAVKR AGNSLALLIQ RDDAQ

Validated NT Sequence

tctgggcgtc gtcgcgctgg atcagcagcg caaggctgtt gcccgcgcgc ttgaccgcgt cgcgcaattg ttcggcgctc gtgacgggcc gcccgttcac cgcgaggatc acgtcgcccg gctggatgcc cgcgcttgcg gcggggcccg tcgactgctg gacgacgagg ccgtgcgtga gcgacgtgcc gttgcgctcg cgcggcaaaa gcggccgcac cgcgacgccc aggcgtccct gctcgacggg ctcgtcggcg cccgccttcg cctgatcgtc ggccagcgac gcaagcgtca ccgacactgt cttcttcgac ttgtctcgcc agatctgcag gtcggccttc gtgcccggct tcatgctcgc gatctgcgcg ggcaacgtcg tcgagtcctg aaccggcacg ccgtcgaccg cgaggatcac gtcgcccggc tgcagccccg ccttcgcggc cggccccttc gggtcgaccg agctgacgag cgcgccgtcg ggcttttgca ggccgaacga gctcgcgagc gtctggttga gcccctgcac cgcgacgccg agccggccgc ggctcacgtg gcccgtcttc acgagctcgt ccttcacctt catcgcctcg ttgatcggga tcgcgaacga caggccctgg aagccgcccg tctgcgagta gatcatcgag ttgatgccga tcacctcgcc gttcangttg aagagcggac cgcccgagtt gccggggttc accggcacgt cggtctggat gaacggcgtg tagttctcgt cgggcagcgc gcgcgacttc gcgctgatga tgcccgacgt gaccgtgttg tcgaacccgt acggcgaacc gatcgcgacg acccactggc cgaccttgct ctgcgccgga tcgccgatct tcacggtcgg caggcccgac gcgtcaatct tcagcaccgc gacgtcggat tgcttgtcgg tgccgacgac cttcgccttg tactcgcgct tgtcggtgag cttcaccgtg acgacgttcg cgccgtcgat cacgtgcgcg ttggtcagga tatacccgtc cgcgctgatg atgaacccgg agcccaggct cgtgctcggc tggtcgtccg gctgcggctg gcggccaccg cccatcccgg gaacctgccc gtagaaatgt cggaagaatt ggtagaacgg atcttccgga tcgatcggca attgcggcgc tgcgcggcgc tgcgcgacgc gctgcacgac gtgcttcgcg ctgatgttca cgacggccgg cccgtaggtc tcgaccaggc cggaaaagtc ggggatgccc gtcttcgcgg ctgcctcggc cggcatcagc gcggcgacgg ccggtgtgat gatctgcggt tc

Expected Protein Sequence

MAHHHHHHMA EPQIITPAVA ALMPAEAAAK TGIPDFSGLV ETYGPAVVNI SAKHVVQRVA QRRAAPQLPI DPEDPFYQFF RHFYGQVPGM GGGRQPQPDD QPSTSLGSGF IISADGYILT NAHVIDGANV VTVKLTDKRE YKAKVVGTDK QSDVAVLKID ASGLPTVKIG DPAQSKVGQW VVAIGSPYGF DNTVTSGIIS AKSRALPDEN YTPFIQTDVP VNPGNSGGPL FNLNGEVIGI NSMIYSQTGG FQGLSFAIPI NEAMKVKDEL VKTGHVSRGR LGVAVQGLNQ TLASSFGLQK PDGALVSSVD PKGPAAKAGL QPGDVILAVD GVPVQDSTTL PAQIASMKPG TKADLQIWRD KSKKTVSVTL ASLADDQAKA GADEPVEQGR LGVAVRPLLP RERNGTSLTH GLVVQQSTGP AASAGIQPGD VILAVNGRPV TSAEQLRDAV KRAGNSLALL IQRDDAQIFV PVDLG

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcgg aaccgcagat catcacaccg gccgtcgccg cgctgatgcc ggccgaggca gccgcgaaga cgggcatccc cgacttttcc ggcctggtcg agacctacgg gccggccgtc gtgaacatca gcgcgaagca cgtcgtgcag cgcgtcgcgc agcgccgcgc agcgccgcaa ttgccgatcg atccggaaga tccgttctac caattcttcc gacatttcta cgggcaggtt cccgggatgg gcggtggccg ccagccgcag ccggacgacc agccgagcac gagcctgggc tccgggttca tcatcagcgc ggacgggtat atcctgacca acgcgcacgt gatcgacggc gcgaacgtcg tcacggtgaa gctcaccgac aagcgcgagt acaaggcgaa ggtcgtcggc accgacaagc aatccgacgt cgcggtgctg aagattgacg cgtcgggcct gccgaccgtg aagatcggcg atccggcgca gagcaaggtc ggccagtggg tcgtcgcgat cggttcgccg tacgggttcg acaacacggt cacgtcgggc atcatcagcg cgaagtcgcg cgcgctgccc gacgagaact acacgccgtt catccagacc gacgtgccgg tgaaccccgg caactcgggc ggtccgctct tcaacctgaa cggcgaggtg atcggcatca actcgatgat ctactcgcag acgggcggct tccagggcct gtcgttcgcg atcccgatca acgaggcgat gaaggtgaag gacgagctcg tgaagacggg ccacgtgagc cgcggccggc tcggcgtcgc ggtgcagggg ctcaaccaga cgctcgcgag ctcgttcggc ctgcaaaagc ccgacggcgc gctcgtcagc tcggtcgacc cgaaggggcc ggccgcgaag gcggggctgc agccgggcga cgtgatcctc gcggtcgacg gcgtgccggt tcaggactcg acgacgttgc ccgcgcagat cgcgagcatg aagccgggca cgaaggccga cctgcagatc tggcgagaca agtcgaagaa gacagtgtcg gtgacgcttg cgtcgctggc cgacgatcag gcgaaggcgg gcgccgacga gcccgtcgag cagggacgcc tgggcgtcgc ggtgcggccg cttttgccgc gcgagcgcaa cggcacgtcg ctcacgcacg gcctcgtcgt ccagcagtcg acgggccccg ccgcaagcgc gggcatccag ccgggcgacg tgatcctcgc ggtgaacggg cggcccgtca cgagcgccga acaattgcgc gacgcggtca agcgcgcggg caacagcctt gcgctgctga tccagcgcga cgacgcccag attttcgtgc cggtcgatct gggctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for ButhA.10100.c.B2.PS01831

PURIFICATION DATe:	5/23/2013
CONCENTRATION:	8.9mg/ml
OBSERVED MW:	50kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	10
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	93

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCGG AACCGCAGAT CATCACACCG GCCGTCGCCG CGCTGATGCC GGCCGAGGCA GCCGCGAAGA CGGGCATCCC CGACTTTTCC GGCCTGGTCG AGACCTACGG GCCGGCCGTC GTGAACATCA GCGCGAAGCA CGTCGTGCAG CGCGTCGCGC AGCGCCGCGC AGCGCCGCAA TTGCCGATCG ATCCGGAAGA TCCGTTCTAC CAATTCTTCC GACATTTCTA CGGGCAGGTT CCCGGGATGG GCGGTGGCCG CCAGCCGCAG CCGGACGACC AGCCGAGCAC GAGCCTGGGC TCCGGGTTCA TCATCAGCGC GGACGGGTAT ATCCTGACCA ACGCGCACGT GATCGACGGC GCGAACGTCG TCACGGTGAA GCTCACCGAC AAGCGCGAGT ACAAGGCGAA GGTCGTCGGC ACCGACAAGC AATCCGACGT CGCGGTGCTG AAGATTGACG CGTCGGGCCT GCCGACCGTG AAGATCGGCG ATCCGGCGCA GAGCAAGGTC GGCCAGTGGG TCGTCGCGAT CGGTTCGCCG TACGGGTTCG ACAACACGGT CACGTCGGGC ATCATCAGCG CGAAGTCGCG CGCGCTGCCC GACGAGAACT ACACGCCGTT CATCCAGACC GACGTGCCGG TGAACCCCGG CAACTCGGGC GGTCCGCTCT TCAACCTGAA CGGCGAGGTG ATCGGCATCA ACTCGATGAT CTACTCGCAG ACGGGCGGCT TCCAGGGCCT GTCGTTCGCG ATCCCGATCA ACGAGGCGAT GAAGGTGAAG GACGAGCTCG TGAAGACGGG CCACGTGAGC CGCGGCCGGC TCGGCGTCGC GGTGCAGGGG CTCAACCAGA CGCTCGCGAG CTCGTTCGGC CTGCAAAAGC CCGACGGCGC GCTCGTCAGC TCGGTCGACC CGAAGGGGCC GGCCGCGAAG GCGGGGCTGC AGCCGGGCGA CGTGATCCTC GCGGTCGACG GCGTGCCGGT TCAGGACTCG ACGACGTTGC CCGCGCAGAT CGCGAGCATG AAGCCGGGCA CGAAGGCCGA CCTGCAGATC TGGCGAGACA AGTCGAAGAA GACAGTGTCG GTGACGCTTG CGTCGCTGGC CGACGATCAG GCGAAGGCGG GCGCCGACGA GCCCGTCGAG CAGGGACGCC TGGGCGTCGC GGTGCGGCCG CTTTTGCCGC GCGAGCGCAA CGGCACGTCG CTCACGCACG GCCTCGTCGT CCAGCAGTCG ACGGGCCCCG CCGCAAGCGC GGGCATCCAG CCGGGCGACG TGATCCTCGC GGTGAACGGG CGGCCCGTCA CGAGCGCCGA ACAATTGCGC GACGCGGTCA AGCGCGCGGG CAACAGCCTT GCGCTGCTGA TCCAGCGCGA CGACGCCCAG ATTTTCGTGC CGGTCGATCT GGGCTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT