SSGCID: Target Page

ButhA.10100.b

Serine protease, MucD

CENTER ID:	ButhA.10100.b
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.10100.b.B2.GE36205	Mature protein( ButhA.10100.b )		20	485

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.10100.b.B2.PS01807	Mature protein( ButhA.10100.b )		20	485

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_126815
PATRIC ID:	fig\|271848.6.peg.4515
RefSeq:	YP_442261.1
UniProt:	Q2SXT8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTPLAAQSAT AASNVTTAPA ATGAAPTTRA GLPDFADLVE RVGPAVVNIR TTANVPADTR GALPPGLDNG DMSEFFRRFF GIPLPQPPGG QKNAPSAPDA PDTEQNRGVG SGFILSPDGY VMTNAHVVDD ADTIYVTLTD KREFKAKLIG VDERTDVAIV KINASSLPTV AIGDSNRVRV GEWVVAIGSP FGLDNTVTAG IVSAKGRNTG DYLPFIQTDV AVNPGNSGGP LINMQGEVIG INSQIYSRTG GFMGISFAIP IDEAMRVAEQ LKASGKVTRG RIAVAIGEVT KEVADSIGLP KAEGALVSSV ESGGPADKAG LQPGDIILKF NGRSVETASD LPRMVGDTKP GTKATVTVWR KGQSRDLPIT IAEFPADKIA KASSRQAPQQ KPRSSALGLA VSDLSPEQLK TLKLRNGVQI DAVDGPAARA GLQRGDIVLR VGDVDISSAK QFVDVTSKLD PQRAVAVLVR RGDNTQFIPI RPRQK

NT Sequence

TTGACGCCGC TTGCCGCGCA ATCGGCGACG GCGGCTTCGA ATGTCACGAC CGCGCCTGCC GCCACGGGCG CCGCGCCCAC GACGCGCGCC GGTTTGCCCG ATTTCGCGGA CCTCGTCGAG AGGGTCGGCC CGGCCGTCGT CAACATCCGG ACGACGGCGA ACGTGCCGGC CGATACGCGC GGCGCGCTGC CGCCCGGCCT CGACAACGGC GACATGTCGG AGTTCTTCCG CCGCTTCTTC GGCATTCCGC TGCCGCAGCC GCCGGGCGGG CAGAAGAATG CGCCGAGCGC GCCCGACGCA CCGGACACCG AGCAGAACCG CGGCGTCGGC TCGGGCTTCA TCCTGTCGCC GGACGGGTAC GTGATGACGA ACGCGCATGT CGTCGACGAC GCGGACACGA TCTACGTGAC GCTCACCGAC AAGCGCGAAT TCAAGGCGAA GCTCATCGGC GTCGACGAGC GCACCGACGT CGCGATCGTG AAGATCAACG CGTCGAGCCT GCCGACCGTT GCGATCGGCG ATTCGAACCG CGTGCGCGTC GGCGAATGGG TCGTCGCGAT CGGTTCGCCG TTCGGTCTCG ACAACACGGT CACGGCCGGC ATCGTCAGCG CGAAGGGCCG CAACACCGGC GACTATCTGC CGTTCATCCA GACGGACGTC GCGGTCAACC CCGGCAACTC GGGCGGCCCG CTCATCAACA TGCAGGGCGA GGTGATCGGC ATCAACTCGC AGATCTACAG CCGCACGGGC GGTTTCATGG GGATCTCGTT CGCGATTCCG ATCGACGAGG CGATGCGCGT CGCCGAGCAG CTGAAAGCGT CGGGCAAGGT CACGCGCGGC CGGATCGCGG TCGCGATCGG CGAGGTGACG AAGGAAGTCG CCGATTCGAT CGGTTTGCCG AAGGCCGAAG GCGCGCTTGT CAGCAGCGTC GAGTCGGGCG GTCCGGCCGA CAAGGCGGGC CTCCAGCCGG GCGACATCAT CCTGAAGTTC AACGGCCGTT CGGTCGAAAC GGCGTCGGAT CTGCCGCGCA TGGTCGGCGA CACGAAGCCG GGCACGAAGG CGACGGTGAC GGTGTGGCGC AAGGGGCAGT CGCGCGATTT GCCGATCACG ATCGCGGAAT TCCCGGCCGA CAAGATCGCG AAGGCAAGCA GCCGCCAGGC GCCGCAGCAG AAGCCGCGCA GCAGCGCGCT CGGCCTGGCG GTCAGCGATC TGTCGCCCGA GCAGTTGAAG ACGCTCAAGC TGCGCAACGG CGTGCAGATC GACGCGGTCG ACGGCCCGGC CGCGCGCGCG GGGCTGCAGC GCGGCGATAT CGTGCTGCGC GTCGGCGACG TCGACATCTC GAGCGCGAAG CAGTTCGTCG ACGTGACGTC GAAGCTCGAT CCTCAGCGCG CGGTCGCGGT GCTCGTGCGG CGCGGCGACA ACACGCAGTT CATCCCGATC CGGCCGCGCC AGAAG

Details for ButhA.10100.b.B2.GE36205

HARVESTED ON:	1/23/2013
SEQUENCED ON:	1/23/2013
EXPECTED MW:	50kDa
OBSERVED MW:	50kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	85

Validated AA Sequence

MSEFFRRFFG IPLPQPPGGQ KNAPSAPDAP DTEQNRGVGS GFILSPDGYV MTNAHVVDDA DTIYVTLTDK REFKAKLIGV DERTDVAIVK INASSLPTVA IGDSNRVRVG EWVVAIGSPF GLDNTVTAGI VSAKGRNTGD YLPFIQTDVA VNPGNSGGPL INMQGEVIGI NSQIYSRTGG FMGISFAIPI DEAMRVAEQL KASGKVTRGR IAVAIGEVTK EVADSIGLPK AEGALVSSVE SGGPADKAGL QPGDIILKFX GRSVETASDL PRMVGDTKPG TKATVTVWRK GQSRDLPITI AEFPADKIXK ASSRQAPQQK PRSSALGLAV SDLSPEQLKT LKLRNGVQID AVDGPAARAG XQRGDIVLRV GDVDISSAKQ FVDXTSKLDP QRAVAVLVRR GDNTQ

Validated NT Sequence

gaactgcgtg ttgtcgccgc gccgcacgag caccgcgacc gcgcgctgag gatcgagctt cgacgtcncg tcnacgaact gcttcgcgct cgagatgtcg acgtcgccga cgcgcagcac gatatcgccg cgctgcnncc ccgcgcgcgc ggccgggccg tcnaccgcgt cgatctgcac gccgttgcgc agcttgagcg tcttcaactg ctcgggcgac agatcgctga ccgccaggcc gagcgcgctg ctgcgcggct tctgctgcgg cgcctggcgg ctgcttgcct tcncgatctt gtcggccggg aattccgcga tcgtgatcgg caaatcgcgc gactgcccct tgcgccacac cgtcaccgtc gccttcgtgc ccggcttcgt gtcgccnacc atgcgcggca gatccgacgc cgtttcgacc gaacggccgn tgaacttcag gatgatgtcg cccggctgga ggcccgcctt gtcngccgga ccgcccgact cgacgctgct gacaagcgcg ccttcggcct tcggcaaacc gatcgaatcg gcgacttcct tcgtcacctc gccgatcgcg accgcgatcc ggccgcgcgt gaccttgccc gacgctttca gctgctcggc gacgcgcatc gcctcgtcga tcggaatcgc gaacgagatc cccatgaaac cgcccgtgcg gctgtagatc tgcgagttga tgccgatcac ctcgccctgc atgttgatga gcgggccgcc cgagttgccg gggttgaccg cgacgtccgt ctggatgaac ggcagatagt cgccggtgtt gcggcccttc gcgctgacga tgccggccgt gaccgtgttg tcgagaccga acggcgaacc gatcgcgacg acccattcgc cgacgcgcac gcggttcgaa tcgccgatcg caacggtcgg caggctcgac gcgttgatct tcacgatcgc gacgtcggtg cgctcgtcga cgccgatgag cttcgccttg aattcgcgct tgtcggtgag cgtcacgtag atcgtgtccg cgtcgtcgac gacatgcgcg ttcgtcatca cgtacccgtc cggcgacagg atgaagcccg agccgacgcc gcggttctgc tcggtgtccg gtgcgtcggg cgcgctcggc gcattcttct gcccgcccgg cggctgcggc agcggaatgc cgaagaagcg gcggaagaac tccgacatgt cgccgttgtc gaggccgggc ggcagcgcgc cgcgcgtatc ggccggcacg ttcgccgtcg tccggatgtt gacgacggcc gggccgaccc tctcgacgag gtccgcgaaa tcgggcaaac cggcgcgcgt cgtgggcgcg gcgcccgt

Expected Protein Sequence

MAHHHHHHMA ATGAAPTTRA GLPDFADLVE RVGPAVVNIR TTANVPADTR GALPPGLDNG DMSEFFRRFF GIPLPQPPGG QKNAPSAPDA PDTEQNRGVG SGFILSPDGY VMTNAHVVDD ADTIYVTLTD KREFKAKLIG VDERTDVAIV KINASSLPTV AIGDSNRVRV GEWVVAIGSP FGLDNTVTAG IVSAKGRNTG DYLPFIQTDV AVNPGNSGGP LINMQGEVIG INSQIYSRTG GFMGISFAIP IDEAMRVAEQ LKASGKVTRG RIAVAIGEVT KEVADSIGLP KAEGALVSSV ESGGPADKAG LQPGDIILKF NGRSVETASD LPRMVGDTKP GTKATVTVWR KGQSRDLPIT IAEFPADKIA KASSRQAPQQ KPRSSALGLA VSDLSPEQLK TLKLRNGVQI DAVDGPAARA GLQRGDIVLR VGDVDISSAK QFVDVTSKLD PQRAVAVLVR RGDNTQFIPI RPRQK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggccg ccacgggcgc cgcgcccacg acgcgcgccg gtttgcccga tttcgcggac ctcgtcgaga gggtcggccc ggccgtcgtc aacatccgga cgacggcgaa cgtgccggcc gatacgcgcg gcgcgctgcc gcccggcctc gacaacggcg acatgtcgga gttcttccgc cgcttcttcg gcattccgct gccgcagccg ccgggcgggc agaagaatgc gccgagcgcg cccgacgcac cggacaccga gcagaaccgc ggcgtcggct cgggcttcat cctgtcgccg gacgggtacg tgatgacgaa cgcgcatgtc gtcgacgacg cggacacgat ctacgtgacg ctcaccgaca agcgcgaatt caaggcgaag ctcatcggcg tcgacgagcg caccgacgtc gcgatcgtga agatcaacgc gtcgagcctg ccgaccgttg cgatcggcga ttcgaaccgc gtgcgcgtcg gcgaatgggt cgtcgcgatc ggttcgccgt tcggtctcga caacacggtc acggccggca tcgtcagcgc gaagggccgc aacaccggcg actatctgcc gttcatccag acggacgtcg cggtcaaccc cggcaactcg ggcggcccgc tcatcaacat gcagggcgag gtgatcggca tcaactcgca gatctacagc cgcacgggcg gtttcatggg gatctcgttc gcgattccga tcgacgaggc gatgcgcgtc gccgagcagc tgaaagcgtc gggcaaggtc acgcgcggcc ggatcgcggt cgcgatcggc gaggtgacga aggaagtcgc cgattcgatc ggtttgccga aggccgaagg cgcgcttgtc agcagcgtcg agtcgggcgg tccggccgac aaggcgggcc tccagccggg cgacatcatc ctgaagttca acggccgttc ggtcgaaacg gcgtcggatc tgccgcgcat ggtcggcgac acgaagccgg gcacgaaggc gacggtgacg gtgtggcgca aggggcagtc gcgcgatttg ccgatcacga tcgcggaatt cccggccgac aagatcgcga aggcaagcag ccgccaggcg ccgcagcaga agccgcgcag cagcgcgctc ggcctggcgg tcagcgatct gtcgcccgag cagttgaaga cgctcaagct gcgcaacggc gtgcagatcg acgcggtcga cggcccggcc gcgcgcgcgg ggctgcagcg cggcgatatc gtgctgcgcg tcggcgacgt cgacatctcg agcgcgaagc agttcgtcga cgtgacgtcg aagctcgatc ctcagcgcgc ggtcgcggtg ctcgtgcggc gcggcgacaa cacgcagttc atcccgatcc ggccgcgcca gaagtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for ButhA.10100.b.B2.PS01807

PURIFICATION DATe:	4/14/2013
CONCENTRATION:	14.5mg/ml
OBSERVED MW:	49kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	6
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	85

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCCG CCACGGGCGC CGCGCCCACG ACGCGCGCCG GTTTGCCCGA TTTCGCGGAC CTCGTCGAGA GGGTCGGCCC GGCCGTCGTC AACATCCGGA CGACGGCGAA CGTGCCGGCC GATACGCGCG GCGCGCTGCC GCCCGGCCTC GACAACGGCG ACATGTCGGA GTTCTTCCGC CGCTTCTTCG GCATTCCGCT GCCGCAGCCG CCGGGCGGGC AGAAGAATGC GCCGAGCGCG CCCGACGCAC CGGACACCGA GCAGAACCGC GGCGTCGGCT CGGGCTTCAT CCTGTCGCCG GACGGGTACG TGATGACGAA CGCGCATGTC GTCGACGACG CGGACACGAT CTACGTGACG CTCACCGACA AGCGCGAATT CAAGGCGAAG CTCATCGGCG TCGACGAGCG CACCGACGTC GCGATCGTGA AGATCAACGC GTCGAGCCTG CCGACCGTTG CGATCGGCGA TTCGAACCGC GTGCGCGTCG GCGAATGGGT CGTCGCGATC GGTTCGCCGT TCGGTCTCGA CAACACGGTC ACGGCCGGCA TCGTCAGCGC GAAGGGCCGC AACACCGGCG ACTATCTGCC GTTCATCCAG ACGGACGTCG CGGTCAACCC CGGCAACTCG GGCGGCCCGC TCATCAACAT GCAGGGCGAG GTGATCGGCA TCAACTCGCA GATCTACAGC CGCACGGGCG GTTTCATGGG GATCTCGTTC GCGATTCCGA TCGACGAGGC GATGCGCGTC GCCGAGCAGC TGAAAGCGTC GGGCAAGGTC ACGCGCGGCC GGATCGCGGT CGCGATCGGC GAGGTGACGA AGGAAGTCGC CGATTCGATC GGTTTGCCGA AGGCCGAAGG CGCGCTTGTC AGCAGCGTCG AGTCGGGCGG TCCGGCCGAC AAGGCGGGCC TCCAGCCGGG CGACATCATC CTGAAGTTCA ACGGCCGTTC GGTCGAAACG GCGTCGGATC TGCCGCGCAT GGTCGGCGAC ACGAAGCCGG GCACGAAGGC GACGGTGACG GTGTGGCGCA AGGGGCAGTC GCGCGATTTG CCGATCACGA TCGCGGAATT CCCGGCCGAC AAGATCGCGA AGGCAAGCAG CCGCCAGGCG CCGCAGCAGA AGCCGCGCAG CAGCGCGCTC GGCCTGGCGG TCAGCGATCT GTCGCCCGAG CAGTTGAAGA CGCTCAAGCT GCGCAACGGC GTGCAGATCG ACGCGGTCGA CGGCCCGGCC GCGCGCGCGG GGCTGCAGCG CGGCGATATC GTGCTGCGCG TCGGCGACGT CGACATCTCG AGCGCGAAGC AGTTCGTCGA CGTGACGTCG AAGCTCGATC CTCAGCGCGC GGTCGCGGTG CTCGTGCGGC GCGGCGACAA CACGCAGTTC ATCCCGATCC GGCCGCGCCA GAAGTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT