SSGCID: Target Page

ButhA.10100.a

Probable protease signal peptide protein

CENTER ID:	ButhA.10100.a
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.10100.a.B2.GE36204	Mature protein( ButhA.10100.a )		33	502

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.10100.a.B2.PS01790	Mature protein( ButhA.10100.a )		33	502

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.5519
RefSeq:	YP_443173.1
UniProt:	Q2SV76

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSRQTFGRAL IHAAVLGAAL AGFACLRPAE VAAGTLSPSA KAKRIAQPGP AGPIDFPTLV ERYGPAVVSV SVPAQDPQMS ASGLEALDPD DPFFAYFKSA AMPPPASQDN VPRAMAGAGS GFIVSADGLI LTTAYVVGQA SEATVRLIDR REFKARVLAV DDQSDVAVLQ IDATKLPTVR LGDSSRVRVG EPVLTIGTPD GSANTVTTGI VSATSRTLPD GSRFPFFQTD VTGNLDNSGG PVFNRAGEVI GIDVQIYGSG DRNPDVTFAI PIGMAAKVRA QVLQAQPPAQ PAQRAAAQNG LGVDVQDVGP GLAAAFGLPR PAGALVNAVE PGSPAAAVGL KPGDVIVQVG DRPLGRSSEL AADVAALPPA ASVPITLVRN RMPMTVMLGA GAAAGASPAA ASANAGAGSS EAGGADRFGL TMHPLTDDER RSTGLPVGMM VDAVRGPAEN AGIRPGDVVL EFDDTLIETP DMVPALEAKA GKAVAVLIQR GNERKFVSVR SR

NT Sequence

GTGTCTCGTC AAACGTTTGG CCGCGCCCTG ATTCATGCCG CAGTGCTCGG GGCGGCGCTC GCCGGCTTTG CGTGCCTGCG GCCGGCGGAG GTCGCAGCGG GCACGCTGTC TCCGTCCGCG AAGGCGAAGC GGATCGCGCA GCCCGGCCCC GCAGGGCCGA TCGATTTCCC CACGCTCGTC GAGCGATACG GACCCGCGGT GGTCAGCGTG AGCGTGCCCG CCCAGGACCC GCAGATGTCG GCGTCCGGTC TCGAGGCGCT CGATCCCGAC GATCCGTTCT TCGCGTACTT CAAGTCCGCC GCGATGCCGC CGCCCGCGTC GCAGGACAAC GTACCGCGCG CGATGGCGGG CGCCGGGTCC GGTTTCATCG TCAGCGCCGA CGGGCTCATC CTGACGACCG CCTACGTGGT CGGGCAGGCG AGCGAAGCGA CGGTCCGGCT GATCGACCGG CGCGAATTCA AGGCGCGGGT GCTCGCGGTC GACGACCAGA GCGACGTGGC CGTGCTGCAG ATCGACGCGA CGAAGTTGCC GACCGTGCGG CTCGGCGATT CGTCGCGCGT GCGCGTCGGC GAGCCCGTGC TGACGATCGG CACGCCCGAC GGCTCGGCGA ACACTGTGAC GACGGGCATC GTCAGCGCGA CGTCGCGCAC GCTGCCCGAC GGCAGCCGTT TCCCGTTCTT CCAGACCGAC GTGACCGGCA ACCTCGACAA CTCGGGCGGT CCGGTGTTCA ACCGCGCGGG CGAGGTGATC GGCATCGACG TGCAGATCTA CGGAAGCGGC GATCGCAATC CGGACGTGAC GTTCGCGATC CCGATCGGCA TGGCGGCCAA GGTGCGCGCG CAGGTGCTGC AGGCGCAGCC GCCTGCTCAG CCGGCGCAGC GCGCGGCTGC GCAAAACGGG CTTGGCGTCG ACGTGCAGGA CGTGGGCCCC GGGCTCGCGG CCGCGTTCGG CTTGCCGCGG CCGGCGGGCG CGCTCGTCAA CGCGGTCGAG CCGGGGTCGC CCGCGGCCGC GGTCGGCCTG AAGCCGGGCG ACGTGATCGT GCAGGTGGGC GACCGGCCGC TCGGCCGCTC GTCCGAGCTC GCCGCCGACG TGGCGGCGCT GCCGCCTGCG GCGAGCGTGC CGATCACGCT GGTCCGCAAC CGGATGCCGA TGACGGTGAT GCTCGGCGCG GGCGCGGCGG CGGGCGCATC GCCGGCGGCG GCCTCGGCGA ACGCGGGCGC GGGCAGCAGC GAGGCGGGCG GCGCGGACCG CTTCGGCCTG ACGATGCATC CGCTGACGGA CGACGAGCGC CGCTCGACGG GGCTGCCCGT CGGGATGATG GTCGATGCGG TGCGCGGGCC GGCGGAGAAT GCCGGCATCC GGCCGGGCGA CGTCGTGCTG GAGTTCGACG ACACGCTGAT CGAGACGCCG GACATGGTGC CCGCGTTGGA GGCGAAGGCC GGCAAGGCGG TCGCGGTGCT GATTCAGCGG GGAAACGAGC GGAAGTTCGT GTCGGTGCGC TCGAGG

Details for ButhA.10100.a.B2.GE36204

HARVESTED ON:	1/23/2013
SEQUENCED ON:	1/24/2013
EXPECTED MW:	49kDa
OBSERVED MW:	48kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	39

Validated AA Sequence

Validated NT Sequence

ataccatggc tcaccaccac caccaccata tggcgggcac gctgtctccg tccgcgaagg cgaagcggat cgcgcagccc ggccccgcag ggccgatcga tttccccacg ctcgtcgagc gatacggacc cgcggtggtc agcgtgagcg tgcccgccca ggacccgcag atgtcggcgt ccggtctcga ggcgctcgat cccgacgatc cgttcttcgc gtacttcaag tccgccgcga tgccgccgcc cgcgtcgcag gacaacgtac cgcgcgcgat ggcgggcgcc gggtccggtt tcatcgtcag cgccgacggg ctcatcctga cgaccgccta cgtggtcggg caggcgagcg aagcgacggt ccggctgatc gaccggcgcg aattcaaggc gcgggtgctc gcggtcgacg accagagcga cgtggccgtg ctgcagatcg acgcgacgaa gttgccgacc gtgcggctcg gcgattcgtc gcgcgtgcgc gtcggcgagc ccgtgctgac gatcggcacg cccgacggct cggcgaacac tgtgacgacg ggcatcgtca gcgcgacgt

Expected Protein Sequence

MAHHHHHHMA GTLSPSAKAK RIAQPGPAGP IDFPTLVERY GPAVVSVSVP AQDPQMSASG LEALDPDDPF FAYFKSAAMP PPASQDNVPR AMAGAGSGFI VSADGLILTT AYVVGQASEA TVRLIDRREF KARVLAVDDQ SDVAVLQIDA TKLPTVRLGD SSRVRVGEPV LTIGTPDGSA NTVTTGIVSA TSRTLPDGSR FPFFQTDVTG NLDNSGGPVF NRAGEVIGID VQIYGSGDRN PDVTFAIPIG MAAKVRAQVL QAQPPAQPAQ RAAAQNGLGV DVQDVGPGLA AAFGLPRPAG ALVNAVEPGS PAAAVGLKPG DVIVQVGDRP LGRSSELAAD VAALPPAASV PITLVRNRMP MTVMLGAGAA AGASPAAASA NAGAGSSEAG GADRFGLTMH PLTDDERRST GLPVGMMVDA VRGPAENAGI RPGDVVLEFD DTLIETPDMV PALEAKAGKA VAVLIQRGNE RKFVSVRSR

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcgg gcacgctgtc tccgtccgcg aaggcgaagc ggatcgcgca gcccggcccc gcagggccga tcgatttccc cacgctcgtc gagcgatacg gacccgcggt ggtcagcgtg agcgtgcccg cccaggaccc gcagatgtcg gcgtccggtc tcgaggcgct cgatcccgac gatccgttct tcgcgtactt caagtccgcc gcgatgccgc cgcccgcgtc gcaggacaac gtaccgcgcg cgatggcggg cgccgggtcc ggtttcatcg tcagcgccga cgggctcatc ctgacgaccg cctacgtggt cgggcaggcg agcgaagcga cggtccggct gatcgaccgg cgcgaattca aggcgcgggt gctcgcggtc gacgaccaga gcgacgtggc cgtgctgcag atcgacgcga cgaagttgcc gaccgtgcgg ctcggcgatt cgtcgcgcgt gcgcgtcggc gagcccgtgc tgacgatcgg cacgcccgac ggctcggcga acactgtgac gacgggcatc gtcagcgcga cgtcgcgcac gctgcccgac ggcagccgtt tcccgttctt ccagaccgac gtgaccggca acctcgacaa ctcgggcggt ccggtgttca accgcgcggg cgaggtgatc ggcatcgacg tgcagatcta cggaagcggc gatcgcaatc cggacgtgac gttcgcgatc ccgatcggca tggcggccaa ggtgcgcgcg caggtgctgc aggcgcagcc gcctgctcag ccggcgcagc gcgcggctgc gcaaaacggg cttggcgtcg acgtgcagga cgtgggcccc gggctcgcgg ccgcgttcgg cttgccgcgg ccggcgggcg cgctcgtcaa cgcggtcgag ccggggtcgc ccgcggccgc ggtcggcctg aagccgggcg acgtgatcgt gcaggtgggc gaccggccgc tcggccgctc gtccgagctc gccgccgacg tggcggcgct gccgcctgcg gcgagcgtgc cgatcacgct ggtccgcaac cggatgccga tgacggtgat gctcggcgcg ggcgcggcgg cgggcgcatc gccggcggcg gcctcggcga acgcgggcgc gggcagcagc gaggcgggcg gcgcggaccg cttcggcctg acgatgcatc cgctgacgga cgacgagcgc cgctcgacgg ggctgcccgt cgggatgatg gtcgatgcgg tgcgcgggcc ggcggagaat gccggcatcc ggccgggcga cgtcgtgctg gagttcgacg acacgctgat cgagacgccg gacatggtgc ccgcgttgga ggcgaaggcc ggcaaggcgg tcgcggtgct gattcagcgg ggaaacgagc ggaagttcgt gtcggtgcgc tcgaggtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t

Details for ButhA.10100.a.B2.PS01790

PURIFICATION DATe:	3/21/2013
CONCENTRATION:	14.4mg/ml
OBSERVED MW:	49kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	8
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	39

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCGG GCACGCTGTC TCCGTCCGCG AAGGCGAAGC GGATCGCGCA GCCCGGCCCC GCAGGGCCGA TCGATTTCCC CACGCTCGTC GAGCGATACG GACCCGCGGT GGTCAGCGTG AGCGTGCCCG CCCAGGACCC GCAGATGTCG GCGTCCGGTC TCGAGGCGCT CGATCCCGAC GATCCGTTCT TCGCGTACTT CAAGTCCGCC GCGATGCCGC CGCCCGCGTC GCAGGACAAC GTACCGCGCG CGATGGCGGG CGCCGGGTCC GGTTTCATCG TCAGCGCCGA CGGGCTCATC CTGACGACCG CCTACGTGGT CGGGCAGGCG AGCGAAGCGA CGGTCCGGCT GATCGACCGG CGCGAATTCA AGGCGCGGGT GCTCGCGGTC GACGACCAGA GCGACGTGGC CGTGCTGCAG ATCGACGCGA CGAAGTTGCC GACCGTGCGG CTCGGCGATT CGTCGCGCGT GCGCGTCGGC GAGCCCGTGC TGACGATCGG CACGCCCGAC GGCTCGGCGA ACACTGTGAC GACGGGCATC GTCAGCGCGA CGTCGCGCAC GCTGCCCGAC GGCAGCCGTT TCCCGTTCTT CCAGACCGAC GTGACCGGCA ACCTCGACAA CTCGGGCGGT CCGGTGTTCA ACCGCGCGGG CGAGGTGATC GGCATCGACG TGCAGATCTA CGGAAGCGGC GATCGCAATC CGGACGTGAC GTTCGCGATC CCGATCGGCA TGGCGGCCAA GGTGCGCGCG CAGGTGCTGC AGGCGCAGCC GCCTGCTCAG CCGGCGCAGC GCGCGGCTGC GCAAAACGGG CTTGGCGTCG ACGTGCAGGA CGTGGGCCCC GGGCTCGCGG CCGCGTTCGG CTTGCCGCGG CCGGCGGGCG CGCTCGTCAA CGCGGTCGAG CCGGGGTCGC CCGCGGCCGC GGTCGGCCTG AAGCCGGGCG ACGTGATCGT GCAGGTGGGC GACCGGCCGC TCGGCCGCTC GTCCGAGCTC GCCGCCGACG TGGCGGCGCT GCCGCCTGCG GCGAGCGTGC CGATCACGCT GGTCCGCAAC CGGATGCCGA TGACGGTGAT GCTCGGCGCG GGCGCGGCGG CGGGCGCATC GCCGGCGGCG GCCTCGGCGA ACGCGGGCGC GGGCAGCAGC GAGGCGGGCG GCGCGGACCG CTTCGGCCTG ACGATGCATC CGCTGACGGA CGACGAGCGC CGCTCGACGG GGCTGCCCGT CGGGATGATG GTCGATGCGG TGCGCGGGCC GGCGGAGAAT GCCGGCATCC GGCCGGGCGA CGTCGTGCTG GAGTTCGACG ACACGCTGAT CGAGACGCCG GACATGGTGC CCGCGTTGGA GGCGAAGGCC GGCAAGGCGG TCGCGGTGCT GATTCAGCGG GGAAACGAGC GGAAGTTCGT GTCGGTGCGC TCGAGGTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T