ButhA.10014.m

Copper ABC transporter, ATP-binding protein

CENTER ID: ButhA.10014.m
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.10014.m.B1.GE36203 Full length( ButhA.10014.m ) 1 304
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.5159
RefSeq: YP_442844.1
UniProt: Q2SW55

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIELAGVIKT FGAVRAVDGV DLAIAPGELF GLIGRNGAGK STLFQLMLGL VAADAGDVRI AGVSARDLRF RAIRRTLGYL PENVVLYDNL TGFETLRFFA RLKGVDAAAC APLLAEVGLA HAAARKVREY SKGMRQRLGF AQALLGDPRL LFLDEPTNGL DPAGIRAFYL ALNERRRAGT TIVITSHILA EIQQRVDRLA IVRAGRLQAV GSVDALRGET NLPLTIELVL PDGDTREIEA ALRRAPFGEP TVRDRRIAFA CPRDAKMAAL AALSPLAARI VDVHIHEPSL EDLFLGYAGC EENP
NT Sequence
ATGATCGAGC TGGCAGGCGT CATCAAGACG TTCGGCGCGG TGCGCGCGGT CGACGGCGTC GATCTCGCGA TCGCGCCGGG CGAGCTGTTC GGCCTCATCG GCCGCAACGG CGCGGGCAAG AGCACGCTGT TCCAGCTGAT GCTCGGCCTC GTCGCTGCGG ACGCGGGCGA CGTGCGGATC GCCGGCGTAT CGGCCCGCGA CCTGCGGTTT CGCGCGATCC GGCGCACGCT CGGCTATCTG CCGGAAAACG TCGTGCTCTA CGACAACCTG ACCGGCTTCG AGACGCTGCG CTTCTTCGCG CGCCTGAAAG GCGTCGACGC GGCCGCGTGC GCGCCGCTTC TCGCCGAGGT GGGCCTGGCC CACGCGGCCG CGCGCAAGGT CCGCGAGTAT TCGAAAGGCA TGCGGCAGCG GCTCGGCTTC GCGCAGGCGC TGCTCGGCGA TCCGCGCCTG CTGTTCCTCG ACGAGCCGAC GAACGGGCTC GATCCCGCCG GCATCCGCGC GTTCTACCTC GCGCTGAACG AGCGCCGGCG CGCGGGCACG ACGATCGTCA TCACGTCGCA CATCCTCGCC GAGATCCAGC AGCGCGTCGA TCGCCTCGCG ATCGTGCGCG CCGGCCGGCT GCAGGCGGTG GGCAGCGTCG ACGCACTGCG CGGCGAGACG AACCTGCCGC TCACGATCGA GCTCGTGCTG CCCGACGGCG ACACGCGCGA GATCGAAGCC GCGCTGCGCC GCGCGCCGTT CGGCGAGCCG ACGGTGCGCG ACCGGCGCAT CGCGTTCGCG TGCCCGCGCG ACGCGAAGAT GGCGGCGCTC GCCGCGCTGT CGCCGCTCGC CGCGCGCATC GTCGACGTGC ACATCCACGA GCCGTCGCTC GAAGACCTGT TCCTCGGCTA TGCCGGATGC GAGGAGAACC CA
Details for ButhA.10014.m.B1.GE36203
HARVESTED ON: 1/23/2013
SEQUENCED ON: 1/23/2013
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 88
PERCENT COVERAGE: 92
Validated AA Sequence
MAHHHHHHMI ELAGVIKTFG AVRAVDGVDL AIAPGELFGL IGRNGAGKST LFQLMLGLVA ADAGDVRIAG VSARDLRFRA IRRTLGYLPE NVVLYDNLTG FETLRFFARL KGVDAAACAP LLAEVGLAHA AARKVREYSK GMRQRLGFAQ ALLGDPRLLF LDEPTNGLDP XGXRAFYLAL NERRRAGTTI VITSHILAEI QQRVDRLAIV RAGRLQAVGS VDALRGETNL PLTIELVLPD GDTREIEAAL RRAXRXRAXX XSXXRRXXXR XXXMPGXXXX XXXXXXXXXX
Validated NT Sequence
tnnnnatttt nntnnnttta agaaggagan ataccatggc tcaccaccac caccaccata tgatcgagct ggcaggcgtc atcaagacgt tcggcgcggt gcgcgcggtc gacggcgtcg atctcgcgat cgcgccgggc gagctgttcg gcctcatcgg ccgcaacggc gcgggcaaga gcacgctgtt ccagctgatg ctcggcctcg tcgctgcgga cgcgggcgac gtgcggatcg ccggcgtatc ggcccgcgac ctgcggtttc gcgcgatccg gcgcacgctc ggctatctgc cggaaaacgt cgtgctctac gacaacctga ccggcttcga gacgctgcgc ttcttcgcgc gcctgaaagg cgtcgacgcg gccgcgtgcg cgccgcttct cgccgaggtg ggcctggccc acgcggccgc gcgcaaggtc cgcgagtatt cgaaaggcat gcggcagcgg ctcggcttcg cgcaggcgct gctcggcgat ccgcgcctgc tgttcctcga cgagccgacg aacgggctcg atcccnccgg nntccgcgcg ttctacctcg cgctgaacga gcgccggcgc gcgggcacga cgatcgtcat cacgtcgcac atcctcgccg agatccagca gcgcgtcgat cgcctcgcga tcgtgcgcgc cggccggctg caggcggtgg gcagcgtcga cgcactgcgc ggcgagacga acctgccgct cacgatcgag ctcgtgctgc ccgacggcga cacgcgcgag atcgaagccg cgctgcgccg cgcgnntcgn nnacgngcag annnncnatc cnncnntcgn agacntgann cncggntntg nnnnatgccn ggagnnnnca nnannnnnna nnananannn nnnnnnnnnn nnnnccna
Expected Protein Sequence
MAHHHHHHMI ELAGVIKTFG AVRAVDGVDL AIAPGELFGL IGRNGAGKST LFQLMLGLVA ADAGDVRIAG VSARDLRFRA IRRTLGYLPE NVVLYDNLTG FETLRFFARL KGVDAAACAP LLAEVGLAHA AARKVREYSK GMRQRLGFAQ ALLGDPRLLF LDEPTNGLDP AGIRAFYLAL NERRRAGTTI VITSHILAEI QQRVDRLAIV RAGRLQAVGS VDALRGETNL PLTIELVLPD GDTREIEAAL RRAPFGEPTV RDRRIAFACP RDAKMAALAA LSPLAARIVD VHIHEPSLED LFLGYAGCEE NP
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatcg agctggcagg cgtcatcaag acgttcggcg cggtgcgcgc ggtcgacggc gtcgatctcg cgatcgcgcc gggcgagctg ttcggcctca tcggccgcaa cggcgcgggc aagagcacgc tgttccagct gatgctcggc ctcgtcgctg cggacgcggg cgacgtgcgg atcgccggcg tatcggcccg cgacctgcgg tttcgcgcga tccggcgcac gctcggctat ctgccggaaa acgtcgtgct ctacgacaac ctgaccggct tcgagacgct gcgcttcttc gcgcgcctga aaggcgtcga cgcggccgcg tgcgcgccgc ttctcgccga ggtgggcctg gcccacgcgg ccgcgcgcaa ggtccgcgag tattcgaaag gcatgcggca gcggctcggc ttcgcgcagg cgctgctcgg cgatccgcgc ctgctgttcc tcgacgagcc gacgaacggg ctcgatcccg ccggcatccg cgcgttctac ctcgcgctga acgagcgccg gcgcgcgggc acgacgatcg tcatcacgtc gcacatcctc gccgagatcc agcagcgcgt cgatcgcctc gcgatcgtgc gcgccggccg gctgcaggcg gtgggcagcg tcgacgcact gcgcggcgag acgaacctgc cgctcacgat cgagctcgtg ctgcccgacg gcgacacgcg cgagatcgaa gccgcgctgc gccgcgcgcc gttcggcgag ccgacggtgc gcgaccggcg catcgcgttc gcgtgcccgc gcgacgcgaa gatggcggcg ctcgccgcgc tgtcgccgct cgccgcgcgc atcgtcgacg tgcacatcca cgagccgtcg ctcgaagacc tgttcctcgg ctatgccgga tgcgaggaga acccatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat