ButhA.10014.k

ABC transporter, ATP-binding protein

CENTER ID: ButhA.10014.k
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.10014.k.B1.GE36201 Full length( ButhA.10014.k ) 1 312
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.5220
RefSeq: YP_442901.1
UniProt: Q2SVZ8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIEIERVSRL FGDIAAVDDV SLAVARGTVT ALVGASGSGK STLLRMINRL IAPTSGTIRV DGVDTASVPA ETLRRGIGYV IQGHGLFPHW TVARNIATVP RLLGWRAARI DARVRELLAL FELDYDTYAR KLPHQLSGGQ QQRVGVARAL AAEPAILLMD EPFGALDPII RGKAQDDLAA LQRRLGITIV IVTHDIDEAL RLGDQIAVMD AGRILQAGPP AEILGRPQPG VVERLVAGLD RPLRLLALTR VDALAEPGAA PGEPISGALT LRDAISELLW RGASALPVSS ADGAAARRIT LDAIVAQAKR PA
NT Sequence
ATGATCGAGA TCGAACGCGT GAGCCGGCTG TTCGGCGACA TCGCCGCGGT CGACGACGTG TCGCTCGCGG TTGCGCGCGG CACGGTGACG GCGCTCGTCG GCGCATCGGG CAGCGGCAAG TCGACGCTTC TGCGAATGAT CAACCGGCTG ATCGCGCCGA CGAGCGGGAC GATTCGCGTC GACGGCGTCG ACACCGCGAG CGTGCCCGCC GAGACGCTGC GGCGCGGGAT CGGCTACGTG ATCCAGGGGC ACGGCCTCTT CCCGCACTGG ACGGTCGCGC GCAACATCGC GACCGTGCCG CGCCTGCTCG GCTGGCGCGC GGCGCGCATC GACGCGCGCG TGCGCGAGCT GCTCGCGCTG TTCGAGCTCG ACTACGACAC GTACGCGCGC AAGCTGCCGC ACCAACTGTC GGGCGGCCAG CAGCAGCGCG TCGGCGTCGC GCGCGCGCTC GCGGCCGAGC CCGCGATCCT GCTGATGGAC GAGCCGTTCG GCGCGCTCGA TCCGATCATC CGCGGCAAGG CGCAGGACGA TCTCGCCGCG CTGCAGCGCC GGCTCGGGAT CACGATCGTG ATCGTCACGC ACGACATCGA CGAGGCGCTG CGGCTCGGCG ACCAGATCGC GGTGATGGAC GCGGGGCGCA TCCTGCAGGC GGGCCCGCCC GCCGAGATTC TCGGGCGGCC GCAGCCGGGC GTCGTCGAGC GGCTCGTCGC GGGCCTCGAC CGGCCGCTGC GCCTGCTCGC GCTCACGCGC GTCGACGCGC TTGCCGAACC CGGCGCGGCG CCCGGCGAGC CGATATCGGG CGCGCTCACG CTGCGCGACG CGATCTCCGA GCTGCTGTGG CGCGGCGCGA GCGCGCTGCC CGTATCGAGC GCGGACGGCG CGGCGGCCAG GCGCATCACG CTCGATGCGA TCGTCGCGCA GGCGAAGCGG CCGGCA
Details for ButhA.10014.k.B1.GE36201
HARVESTED ON: 1/23/2013
SEQUENCED ON: 1/24/2013
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMI EIERVSRLFG DIAAVDDVSL AVARGTVTAL VGASGSGKST LLRMINRLIA PTSGTIRVDG VDTASVPAET LRRGIGYVIQ GHXLFPHXTV ARNIATVPRL LGWRAARIDA RVRELLALFE LDYDTYARKL PHQLSGGQQQ RVGVARALAA EPAILLMDEP FGALDPIIRG KAQDDLAALQ RRLGITIVIV THDIDEALRL GDQIAVMDAG RILQAGPPAE ILGRPQPGVV ERLVAGLDRP LRLLALTRVD ALAEPGAAPG EPISGALTLR DAISELLWRG ASALPVSSAD GAAARRITLD A
Validated NT Sequence
aggaganata ccatggctca ccaccaccac caccatatga tcgagatcga acgcgtgagc cggctgttcg gcgacatcgc cgcggtcgac gacgtgtcgc tcgcggttgc gcgcggcacg gtgacggcgc tcgtcggcgc atcgggcagc ggcaagtcga cgcttctgcg aatgatcaac cggctgatcg cgccgacgag cgggacgatt cgcgtcgacg gcgtcgacac cgcgagcgtg cccgccgaga cgctgcggcg cgggatcggc tacgtgatcc aggggcacgn cctcttcccg cacnggacgg tcgcgcgcaa catcgcgacc gtgccgcgcc tgctcggctg gcgcgcggcg cgcatcgacg cgcgcgtgcg cgagctgctc gcgctgttcg agctcgacta cgacacgtac gcgcgcaagc tgccgcacca actgtcgggc ggccagcagc agcgcgtcgg cgtcgcgcgc gcgctcgcgg ccgagcccgc gatcctgctg atggacgagc cgttcggcgc gctcgatccg atcatccgcg gcaaggcgca ggacgatctc gccgcgctgc agcgccggct cgggatcacg atcgtgatcg tcacgcacga catcgacgag gcgctgcggc tcggcgacca gatcgcggtg atggacgcgg ggcgcatcct gcaggcgggc ccgcccgccg agattctcgg gcggccgcag ccgggcgtcg tcgagcggct cgtcgcgggc ctcgaccggc cgctgcgcct gctcgcgctc acgcgcgtcg acgcgcttgc cgaacccggc gcggcgcccg gcgagccgat atcgggcgcg ctcacgctgc gcgacgcgat ctccgagctg ctgtggcgcg gcgcgagcgc gctgcccgta tcgagcgcgg acggcgcggc ggccaggcgc atcacgctcg atgcga
Expected Protein Sequence
MAHHHHHHMI EIERVSRLFG DIAAVDDVSL AVARGTVTAL VGASGSGKST LLRMINRLIA PTSGTIRVDG VDTASVPAET LRRGIGYVIQ GHGLFPHWTV ARNIATVPRL LGWRAARIDA RVRELLALFE LDYDTYARKL PHQLSGGQQQ RVGVARALAA EPAILLMDEP FGALDPIIRG KAQDDLAALQ RRLGITIVIV THDIDEALRL GDQIAVMDAG RILQAGPPAE ILGRPQPGVV ERLVAGLDRP LRLLALTRVD ALAEPGAAPG EPISGALTLR DAISELLWRG ASALPVSSAD GAAARRITLD AIVAQAKRPA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatcg agatcgaacg cgtgagccgg ctgttcggcg acatcgccgc ggtcgacgac gtgtcgctcg cggttgcgcg cggcacggtg acggcgctcg tcggcgcatc gggcagcggc aagtcgacgc ttctgcgaat gatcaaccgg ctgatcgcgc cgacgagcgg gacgattcgc gtcgacggcg tcgacaccgc gagcgtgccc gccgagacgc tgcggcgcgg gatcggctac gtgatccagg ggcacggcct cttcccgcac tggacggtcg cgcgcaacat cgcgaccgtg ccgcgcctgc tcggctggcg cgcggcgcgc atcgacgcgc gcgtgcgcga gctgctcgcg ctgttcgagc tcgactacga cacgtacgcg cgcaagctgc cgcaccaact gtcgggcggc cagcagcagc gcgtcggcgt cgcgcgcgcg ctcgcggccg agcccgcgat cctgctgatg gacgagccgt tcggcgcgct cgatccgatc atccgcggca aggcgcagga cgatctcgcc gcgctgcagc gccggctcgg gatcacgatc gtgatcgtca cgcacgacat cgacgaggcg ctgcggctcg gcgaccagat cgcggtgatg gacgcggggc gcatcctgca ggcgggcccg cccgccgaga ttctcgggcg gccgcagccg ggcgtcgtcg agcggctcgt cgcgggcctc gaccggccgc tgcgcctgct cgcgctcacg cgcgtcgacg cgcttgccga acccggcgcg gcgcccggcg agccgatatc gggcgcgctc acgctgcgcg acgcgatctc cgagctgctg tggcgcggcg cgagcgcgct gcccgtatcg agcgcggacg gcgcggcggc caggcgcatc acgctcgatg cgatcgtcgc gcaggcgaag cggccggcat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat