SSGCID: Target Page

ButhA.10014.h

Nod factor export ATP-binding protein I

CENTER ID:	ButhA.10014.h
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.10014.h.B1.GE36198	Full length( ButhA.10014.h )		1	304

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.5337
RefSeq:	YP_443006.1
UniProt:	Q2SVP3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSVAPIDFQQ VEKRYDDKLV VDGLSFHVQP GECFGLLGPN GAGKTTALKM LLGITHPDAG SISLCGEPVP SRARHARRRV GVVPQFDNLD PDFTVRENLL VFARYFGLAA HEARALVPPL LEFAKLENKA DAKVGELSGG MKRRLTLARA LVNNPDVLVL DEPTTGLDPQ ARHLMWERLR SLLVRGKTIL LTTHFMEEAE RLCHRLCVIE EGRKIAEGAP RTLIESEIGC DVIEIYGPDP AQLREELAPF AERTEISGET LFCYVDDPEP VNARLKGRAG LRYLHRPANL EDVFLRLTGR EMQD

NT Sequence

ATGTCTGTCG CACCCATCGA TTTTCAACAA GTCGAGAAAC GCTACGATGA CAAGCTCGTC GTGGACGGCT TGTCGTTTCA TGTGCAGCCC GGCGAATGCT TCGGCCTGCT CGGCCCGAAC GGCGCCGGCA AGACGACGGC GCTCAAGATG CTGCTCGGCA TCACGCATCC CGACGCGGGT TCGATCAGCC TGTGCGGCGA GCCCGTGCCG TCCCGCGCGC GGCACGCGCG GCGGCGCGTC GGCGTCGTGC CGCAATTCGA CAACCTCGAC CCCGACTTCA CCGTTCGCGA GAACCTGCTC GTGTTCGCCC GCTACTTCGG ACTCGCTGCG CACGAAGCGC GCGCGCTCGT GCCTCCGCTG CTCGAGTTCG CGAAACTCGA AAACAAGGCG GATGCGAAAG TGGGCGAGCT GTCGGGCGGG ATGAAGCGGC GGCTCACGCT CGCGCGGGCG CTCGTCAACA ATCCCGACGT GCTCGTGCTC GACGAGCCGA CGACGGGCCT CGATCCGCAA GCCCGGCATC TGATGTGGGA GCGGCTGCGC TCGCTTCTCG TTCGCGGCAA GACGATCCTG CTCACCACGC ACTTCATGGA AGAGGCCGAG CGCCTGTGCC ACCGCCTGTG CGTGATCGAG GAAGGCCGCA AGATCGCGGA AGGCGCGCCG CGCACGCTGA TCGAATCGGA GATCGGCTGC GACGTGATCG AGATCTACGG GCCCGATCCC GCGCAGCTTC GCGAGGAGCT CGCGCCGTTC GCCGAGCGAA CCGAGATCAG CGGCGAAACG CTCTTTTGCT ACGTCGACGA TCCGGAGCCG GTCAACGCGC GCCTGAAGGG CCGCGCCGGT CTGCGCTATC TGCACCGCCC CGCGAATCTC GAAGATGTAT TCCTGCGGCT GACGGGTCGC GAGATGCAGG AT

Details for ButhA.10014.h.B1.GE36198

HARVESTED ON:	1/23/2013
SEQUENCED ON:	1/23/2013
EXPECTED MW:	35kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMS VAPIDFQQVE KRYDDKLVVD GLSFHVQPGE CFGLLGPNGA GKTTALKMLL GITHPDAGSI SLCGEPVPSR ARHARRRVGV VPQFDNLDPD FTVRENLLVF ARYFGLAAHE ARALVPPLLE FAKLENKADA KVGELSGGMK RRLTLARALV NNPDVLVLDE PTTGLDPQAR HLMWERLRSL LVRGKTILLT THFMEEAERL CHRLCVIEEG RKIAEGAPRT LIESEIGCDV IEIYGPDPAQ LREELAPFAE RTEISGETLF CYVDDPEPVN ARLKGRAGLR YLHRPANLED VFLRLTGREM QD

Validated NT Sequence

tcttactcac ttaatcctgc atctcgcgac ccgtcagccg caggaataca tcttcgagat tcgcggggcg gtgcagatag cgcagaccgg cgcggccctt caggcgcgcg ttgaccggct ccggatcgtc gacgtagcaa aagagcgttt cgccgctgat ctcggttcgc tcggcgaacg gcgcgagctc ctcgcgaagc tgcgcgggat cgggcccgta gatctcgatc acgtcgcagc cgatctccga ttcgatcagc gtgcgcggcg cgccttccgc gatcttgcgg ccttcctcga tcacgcacag gcggtggcac aggcgctcgg cctcttccat gaagtgcgtg gtgagcagga tcgtcttgcc gcgaacgaga agcgagcgca gccgctccca catcagatgc cgggcttgcg gatcgaggcc cgtcgtcggc tcgtcgagca cgagcacgtc gggattgttg acgagcgccc gcgcgagcgt gagccgccgc ttcatcccgc ccgacagctc gcccactttc gcatccgcct tgttttcgag tttcgcgaac tcgagcagcg gaggcacgag cgcgcgcgct tcgtgcgcag cgagtccgaa gtagcgggcg aacacgagca ggttctcgcg aacggtgaag tcggggtcga ggttgtcgaa ttgcggcacg acgccgacgc gccgccgcgc gtgccgcgcg cgggacggca cgggctcgcc gcacaggctg atcgaacccg cgtcgggatg cgtgatgccg agcagcatct tgagcgccgt cgtcttgccg gcgccgttcg ggccgagcag gccgaagcat tcgccgggct gcacatgaaa cgacaagccg tccacgacga gcttgtcatc gtagcgtttc tcgacttgtt gaaaatcgat gggtgcgaca gacatatggt ggtggtggtg gtgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtctg tcgcacccat cgattttcaa caagtcgaga aacgctacga tgacaagctc gtcgtggacg gcttgtcgtt tcatgtgcag cccggcgaat gcttcggcct gctcggcccg aacggcgccg gcaagacgac ggcgctcaag atgctgctcg gcatcacgca tcccgacgcg ggttcgatca gcctgtgcgg cgagcccgtg ccgtcccgcg cgcggcacgc gcggcggcgc gtcggcgtcg tgccgcaatt cgacaacctc gaccccgact tcaccgttcg cgagaacctg ctcgtgttcg cccgctactt cggactcgct gcgcacgaag cgcgcgcgct cgtgcctccg ctgctcgagt tcgcgaaact cgaaaacaag gcggatgcga aagtgggcga gctgtcgggc gggatgaagc ggcggctcac gctcgcgcgg gcgctcgtca acaatcccga cgtgctcgtg ctcgacgagc cgacgacggg cctcgatccg caagcccggc atctgatgtg ggagcggctg cgctcgcttc tcgttcgcgg caagacgatc ctgctcacca cgcacttcat ggaagaggcc gagcgcctgt gccaccgcct gtgcgtgatc gaggaaggcc gcaagatcgc ggaaggcgcg ccgcgcacgc tgatcgaatc ggagatcggc tgcgacgtga tcgagatcta cgggcccgat cccgcgcagc ttcgcgagga gctcgcgccg ttcgccgagc gaaccgagat cagcggcgaa acgctctttt gctacgtcga cgatccggag ccggtcaacg cgcgcctgaa gggccgcgcc ggtctgcgct atctgcaccg ccccgcgaat ctcgaagatg tattcctgcg gctgacgggt cgcgagatgc aggattgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat