SSGCID: Target Page

ButhA.10014.f

Sugar ABC transporter, ATP-binding protein

CENTER ID:	ButhA.10014.f
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.10014.f.B1.GE36196	Full length( ButhA.10014.f )		1	266

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.5505
RefSeq:	YP_443161.1
UniProt:	Q2SV88

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSTPASNDTI LALENVSKYF GKVIALSGVT LRVKRGEVHC LLGDNGAGKS TLIKTLAGVH QPSEGQYLVD GKPVLFDSPK DALDLGIATV YQDLALVPLL SVARNFFMGR EPQKKLFGLF NVMDLDSSAQ IARAKLAEMG INVRDAHQPI GTMSGGERQC LAIARAIHFG ARVLILDEPT AALGVKQSFN VLKLIHTARA KGISVIFITH NVHHAYPIGD SFTLLNRGRS LGTFTKDTIS KNEVLDMMAG GAEMQKMIAE LDGATI

NT Sequence

ATGTCCACGC CAGCATCCAA CGATACGATT CTCGCGCTCG AGAACGTCAG CAAGTATTTC GGCAAGGTGA TCGCGCTGTC CGGCGTCACG CTGCGCGTCA AGCGCGGCGA GGTGCATTGC CTGCTCGGCG ACAACGGCGC CGGCAAGTCC ACGCTCATCA AGACGCTCGC GGGCGTGCAC CAGCCGTCCG AAGGGCAGTA CCTCGTCGAC GGCAAGCCGG TGCTGTTCGA TTCGCCGAAG GACGCGCTCG ACCTCGGCAT CGCGACCGTC TATCAGGACC TCGCGCTCGT GCCGCTCCTG TCCGTCGCGC GCAATTTCTT CATGGGCCGC GAGCCGCAGA AAAAGCTGTT CGGCCTCTTC AACGTGATGG ACCTCGATTC GAGCGCGCAG ATCGCCCGCG CGAAGCTCGC GGAGATGGGC ATCAACGTGC GCGACGCGCA CCAGCCGATC GGCACGATGT CGGGCGGCGA GCGCCAGTGC CTCGCGATCG CGCGCGCGAT CCATTTCGGC GCGCGCGTGC TGATCCTCGA CGAGCCGACC GCCGCGCTCG GCGTCAAGCA GAGCTTCAAC GTGCTCAAGC TGATCCACAC CGCGCGCGCG AAGGGGATCT CGGTGATCTT CATCACGCAC AACGTGCACC ACGCGTACCC GATCGGCGAT TCGTTCACGC TGCTCAACCG CGGCCGCTCG CTCGGCACGT TCACGAAGGA CACGATCAGC AAGAACGAGG TGCTCGACAT GATGGCGGGC GGCGCCGAGA TGCAGAAGAT GATCGCCGAA CTCGACGGCG CGACCATT

Details for ButhA.10014.f.B1.GE36196

HARVESTED ON:	1/23/2013
SEQUENCED ON:	1/23/2013
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMS TPASNDTILA LENVSKYFGK VIALSGVTLR VKRGEVHCLL GDNGAGKSTL IKTLAGVHQP SEGQYLVDGK PVLFDSPKDA LDLGIATVYQ DLALVPLLSV ARNFFMGREP QKKLFGLFNV MDLDSSAQIA RAKLAEMGIN VRDAHQPIGT MSGGERQCLA IARAIHFGAR VLILDEPTAA LGVKQSFNVL KLIHTARAKG ISVIFITHNV HHAYPIGDSF TLLNRGRSLG TFTXDTISKN EVLDMMAGGA EMQKMIAELD GATI

Validated NT Sequence

ttnnnnnntt taagaaggag anataccatg gctcaccacc accaccacca tatgtccacg ccagcatcca acgatacgat tctcgcgctc gagaacgtca gcaagtattt cggcaaggtg atcgcgctgt ccggcgtcac gctgcgcgtc aagcgcggcg aggtgcattg cctgctcggc gacaacggcg ccggcaagtc cacgctcatc aagacgctcg cgggcgtgca ccagccgtcc gaagggcagt acctcgtcga cggcaagccg gtgctgttcg attcgccgaa ggacgcgctc gacctcggca tcgcgaccgt ctatcaggac ctcgcgctcg tgccgctcct gtccgtcgcg cgcaatttct tcatgggccg cgagccgcag aaaaagctgt tcggcctctt caacgtgatg gacctcgatt cgagcgcgca gatcgcccgc gcgaagctcg cggagatggg catcaacgtg cgcgacgcgc accagccgat cggcacgatg tcgggcggcg agcgccagtg cctcgcgatc gcgcgcgcga tccatttcgg cgcgcgcgtg ctgatcctcg acgagccgac cgccgcgctc ggcgtcaagc agagcttcaa cgtgctcaag ctgatccaca ccgcgcgcgc gaaggggatc tcggtgatct tcatcacgca caacgtgcac cacgcgtacc cgatcggcga ttcgttcacg ctgctcaacc gcggccgctc gctcggcacg ttcacgaang acacgatcag caagaacgag gtgctcgaca tgatggcggg cggcgccgag atgcagaaga tgatcgccga actcgacggc gcgaccattt aagtgagtaa gataggatcc gcccccnnnn nnnnnnnnnn annnna

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcca cgccagcatc caacgatacg attctcgcgc tcgagaacgt cagcaagtat ttcggcaagg tgatcgcgct gtccggcgtc acgctgcgcg tcaagcgcgg cgaggtgcat tgcctgctcg gcgacaacgg cgccggcaag tccacgctca tcaagacgct cgcgggcgtg caccagccgt ccgaagggca gtacctcgtc gacggcaagc cggtgctgtt cgattcgccg aaggacgcgc tcgacctcgg catcgcgacc gtctatcagg acctcgcgct cgtgccgctc ctgtccgtcg cgcgcaattt cttcatgggc cgcgagccgc agaaaaagct gttcggcctc ttcaacgtga tggacctcga ttcgagcgcg cagatcgccc gcgcgaagct cgcggagatg ggcatcaacg tgcgcgacgc gcaccagccg atcggcacga tgtcgggcgg cgagcgccag tgcctcgcga tcgcgcgcgc gatccatttc ggcgcgcgcg tgctgatcct cgacgagccg accgccgcgc tcggcgtcaa gcagagcttc aacgtgctca agctgatcca caccgcgcgc gcgaagggga tctcggtgat cttcatcacg cacaacgtgc accacgcgta cccgatcggc gattcgttca cgctgctcaa ccgcggccgc tcgctcggca cgttcacgaa ggacacgatc agcaagaacg aggtgctcga catgatggcg ggcggcgccg agatgcagaa gatgatcgcc gaactcgacg gcgcgaccat ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat