SSGCID: Target Page

ButhA.10014.e

Phosphate import ATP-binding protein pstB

CENTER ID:	ButhA.10014.e
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.10014.e.B1.GE36195	Full length( ButhA.10014.e )		1	282

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.5633
RefSeq:	YP_443282.1
UniProt:	Q2SUW7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNMAESHLDP SKLATGPAGA GAATGQRPLA PLNAKIEVKN LNFFYNQFHA LKNINLSIPE GKVTAFIGPS GCGKSTLLRT FNKMYALYPE QRAEGEIVMD GENLLQSKLD ISLLRARIGM VFQKPTPFPM SIYDNIAFGV KMFERLTRSE MDDRVEWALT KAALWNEVKD KLSQSGYGLS GGQQQRLCIA RGIAIRPEVL LLDEPCSALD PISTGRIEEL IAELKSDYTV VIVTHNMQQA ARCSDYTAYM YLGELIEFGE TEKIFIKPAR KETEDYITGR FG

NT Sequence

ATGAACATGG CAGAAAGCCA CCTCGATCCT TCGAAACTCG CCACGGGGCC GGCCGGCGCC GGCGCGGCGA CGGGGCAGCG TCCGCTCGCG CCGCTGAACG CGAAGATCGA AGTCAAGAAC CTGAATTTCT TCTACAACCA GTTTCACGCG CTGAAGAACA TCAACCTGAG CATTCCCGAA GGGAAGGTGA CGGCGTTCAT CGGCCCGTCG GGCTGCGGCA AGTCCACGCT TTTGCGCACG TTCAACAAGA TGTACGCGCT CTACCCGGAG CAGCGCGCGG AAGGCGAGAT CGTGATGGAC GGCGAGAACC TGCTGCAGTC GAAGCTCGAC ATCTCGCTCT TGCGCGCGCG CATCGGCATG GTATTCCAGA AGCCGACGCC GTTCCCGATG TCGATCTACG ACAACATCGC GTTCGGCGTG AAGATGTTCG AGCGGCTCAC GCGCTCGGAG ATGGACGACC GCGTCGAATG GGCGCTCACG AAGGCGGCGC TCTGGAACGA AGTGAAGGAC AAGCTGAGCC AGAGCGGCTA TGGCCTGTCG GGCGGGCAGC AGCAGCGCCT TTGCATCGCG CGCGGCATCG CGATCCGGCC TGAAGTGCTG CTCCTCGACG AGCCGTGCTC GGCGCTCGAT CCGATCTCGA CGGGCCGCAT CGAAGAGCTG ATCGCGGAGT TGAAGAGCGA CTACACGGTC GTCATCGTCA CGCACAACAT GCAGCAGGCG GCGCGCTGCT CGGACTACAC CGCGTACATG TATCTCGGTG AGCTGATCGA GTTCGGCGAA ACGGAGAAGA TCTTCATCAA GCCGGCGCGC AAGGAAACGG AAGATTACAT CACGGGCCGC TTCGGC

Details for ButhA.10014.e.B1.GE36195

HARVESTED ON:	1/23/2013
SEQUENCED ON:	1/23/2013
EXPECTED MW:	32kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMN MAESHLDPSK LATGPAGAGA ATGQRPLAPL NAKIEVKNLN FFYNQFHALK NINLSIPEGK VTAFIGPSGC GKSTLLRTFN KMYALYPEQR AEGEIVMDGE NLLQSKLDIS LLRARIGMVF QKPTPFPMSI YDNIAFGVKM FERLTRSEMD DRVEWALTKA ALWNEVKDKL SQSGYGLSGG QQQRLCIARG IAIRPEVLLL DEPCSALDPI STGRIEELIA ELKSDYTVVI VTHNMQXAAR CSDYTAYMYL GELIEFGETE KIFIKPARKE TEXYITGRFG

Validated NT Sequence

cnnnnnnnnt tttnntnnnt ttaagaagga gatataccat ggctcaccac caccaccacc atatgaacat ggcagaaagc cacctcgatc cttcgaaact cgccacgggg ccggccggcg ccggcgcggc gacggggcag cgtccgctcg cgccgctgaa cgcgaagatc gaagtcaaga acctgaattt cttctacaac cagtttcacg cgctgaagaa catcaacctg agcattcccg aagggaaggt gacggcgttc atcggcccgt cgggctgcgg caagtccacg cttttgcgca cgttcaacaa gatgtacgcg ctctacccgg agcagcgcgc ggaaggcgag atcgtgatgg acggcgagaa cctgctgcag tcgaagctcg acatctcgct cttgcgcgcg cgcatcggca tggtattcca gaagccgacg ccgttcccga tgtcgatcta cgacaacatc gcgttcggcg tgaagatgtt cgagcggctc acgcgctcgg agatggacga ccgcgtcgaa tgggcgctca cgaaggcggc gctctggaac gaagtgaagg acaagctgag ccagagcggc tatggcctgt cgggcgggca gcagcagcgc ctttgcatcg cgcgcggcat cgcgatccgg cctgaagtgc tgctcctcga cgagccgtgc tcggcgctcg atccgatctc gacgggccgc atcgaagagc tgatcgcgga gttgaagagc gactacacgg tcgtcatcgt cacgcacaac atgcagcang cggcgcgctg ctcggactac accgcgtaca tgtatctcgg tgagctgatc gagttcggcg aaacggagaa gatcttcatc aagccggcgc gcaaggaaac ggaagantac atcacgggcc gcttcggcta agtgagtaan ataggatcnn nnnnnnnnan cc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaca tggcagaaag ccacctcgat ccttcgaaac tcgccacggg gccggccggc gccggcgcgg cgacggggca gcgtccgctc gcgccgctga acgcgaagat cgaagtcaag aacctgaatt tcttctacaa ccagtttcac gcgctgaaga acatcaacct gagcattccc gaagggaagg tgacggcgtt catcggcccg tcgggctgcg gcaagtccac gcttttgcgc acgttcaaca agatgtacgc gctctacccg gagcagcgcg cggaaggcga gatcgtgatg gacggcgaga acctgctgca gtcgaagctc gacatctcgc tcttgcgcgc gcgcatcggc atggtattcc agaagccgac gccgttcccg atgtcgatct acgacaacat cgcgttcggc gtgaagatgt tcgagcggct cacgcgctcg gagatggacg accgcgtcga atgggcgctc acgaaggcgg cgctctggaa cgaagtgaag gacaagctga gccagagcgg ctatggcctg tcgggcgggc agcagcagcg cctttgcatc gcgcgcggca tcgcgatccg gcctgaagtg ctgctcctcg acgagccgtg ctcggcgctc gatccgatct cgacgggccg catcgaagag ctgatcgcgg agttgaagag cgactacacg gtcgtcatcg tcacgcacaa catgcagcag gcggcgcgct gctcggacta caccgcgtac atgtatctcg gtgagctgat cgagttcggc gaaacggaga agatcttcat caagccggcg cgcaaggaaa cggaagatta catcacgggc cgcttcggct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat