ButhA.10014.a

ABC transporter, ATP-binding protein

CENTER ID: ButhA.10014.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
ButhA.10014.a.B1.GE36191 Full length( ButhA.10014.a ) 1 272
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.5892
RefSeq: YP_443508.1
UniProt: Q2SU91

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSSSESLLA LRDVDFGYGE RLVLSNLNMR FARGQVVAVM GGSGCGKTTV LRLIGGLVRA RRGQVLFDGA DVGAQTREGL YALRRKMGML FQFGALFTDM SVYDNVAFAL REHTDLPEEL IRDLVLMKLN AVGLRGAREL MPSEVSGGMA RRIALARAIA LDPQLIMYDE PFAGLDPISL GITANLIRTL NHALGATSIL VTHDVPESFA IADYVYFLAN GGVLAEGTPD ELRASTDPSV RQFIDGAPDG PFKFHYMSQP LAADFGLGGG RR
NT Sequence
GTGAGCTCCT CCTCCGAGTC CCTGCTGGCG CTTCGCGACG TCGATTTCGG CTACGGCGAG CGTCTCGTCC TGTCGAACCT GAACATGCGC TTCGCACGCG GCCAGGTGGT CGCGGTGATG GGCGGTTCAG GCTGCGGCAA GACCACCGTG CTGCGCCTGA TCGGCGGGCT CGTGCGCGCA CGCCGCGGCC AGGTGCTGTT CGACGGCGCC GATGTCGGCG CGCAGACGCG CGAGGGCCTC TACGCGCTGC GCCGCAAGAT GGGCATGCTG TTTCAGTTCG GCGCGCTCTT CACCGACATG TCGGTGTACG ACAACGTCGC GTTCGCGCTG CGCGAGCACA CGGATCTGCC CGAAGAGCTG ATCCGCGATC TCGTGCTGAT GAAGCTCAAC GCGGTCGGCC TGCGCGGCGC GCGCGAGCTG ATGCCGTCCG AGGTGTCGGG CGGGATGGCG CGGCGCATCG CGCTCGCGCG GGCGATCGCG CTCGACCCGC AGCTCATCAT GTACGACGAG CCGTTCGCGG GCCTCGACCC TATTTCGCTC GGCATCACCG CAAACCTGAT CCGCACGCTG AACCATGCGC TCGGCGCGAC GTCGATCCTC GTCACGCACG ACGTGCCGGA ATCGTTCGCG ATCGCGGATT ACGTGTACTT CCTCGCGAAC GGCGGCGTGC TGGCCGAGGG CACGCCCGAC GAGCTGCGTG CGTCGACCGA TCCGAGCGTG CGCCAGTTCA TCGACGGCGC GCCGGACGGC CCGTTCAAAT TTCACTACAT GAGCCAGCCG CTCGCGGCGG ACTTCGGACT TGGCGGAGGG CGCAGA
Details for ButhA.10014.a.B1.GE36191
HARVESTED ON: 1/23/2013
SEQUENCED ON: 1/23/2013
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMV SSSSESLLAL RDVDFGYGER LVLSNLNMRF ARGQVVAVMG GSGCGKTTVL RLIGGLVRAR RGQVLFDGAD VGAQTREGLY ALRRKMGMLF QFGALFTDMS VYDNVAFALR EHTDLPEELI RDLVLMKLNA VGLRGARELM PSEVSGGMAR RIALARAIAL DPQLIMYDEP FAGLDPISLG ITANLIRTLN HALGATSILV THDVPESFAI ADYVYFLANG GVLAEGTPDE LRASTDPSVR QFIDGAPDGP FKFHYMSQPL AADFXLGGXR R
Validated NT Sequence
annnnnnttt nttnnnttta agaaggagan ataccatggc tcaccaccac caccaccata tggtgagctc ctcctccgag tccctgctgg cgcttcgcga cgtcgatttc ggctacggcg agcgtctcgt cctgtcgaac ctgaacatgc gcttcgcacg cggccaggtg gtcgcggtga tgggcggttc aggctgcggc aagaccaccg tgctgcgcct gatcggcggg ctcgtgcgcg cacgccgcgg ccaggtgctg ttcgacggcg ccgatgtcgg cgcgcagacg cgcgagggcc tctacgcgct gcgccgcaag atgggcatgc tgtttcagtt cggcgcgctc ttcaccgaca tgtcggtgta cgacaacgtc gcgttcgcgc tgcgcgagca cacggatctg cccgaagagc tgatccgcga tctcgtgctg atgaagctca acgcggtcgg cctgcgcggc gcgcgcgagc tgatgccgtc cgaggtgtcg ggcgggatgg cgcggcgcat cgcgctcgcg cgggcgatcg cgctcgaccc gcagctcatc atgtacgacg agccgttcgc gggcctcgac cctatttcgc tcggcatcac cgcaaacctg atccgcacgc tgaaccatgc gctcggcgcg acgtcgatcc tcgtcacgca cgacgtgccg gaatcgttcg cgatcgcgga ttacgtgtac ttcctcgcga acggcggcgt gctggccgag ggcacgcccg acgagctgcg tgcgtcgacc gatccgagcg tgcgccagtt catcgacggc gcgccngacg gcccgttcaa atttcactac atgagccagc cgctcgcggc ggacttcnga cttggcggan ggcgcagata agtgagtaag ataggatccg gcnnnnnnna ccccnnnnna a
Expected Protein Sequence
MAHHHHHHMV SSSSESLLAL RDVDFGYGER LVLSNLNMRF ARGQVVAVMG GSGCGKTTVL RLIGGLVRAR RGQVLFDGAD VGAQTREGLY ALRRKMGMLF QFGALFTDMS VYDNVAFALR EHTDLPEELI RDLVLMKLNA VGLRGARELM PSEVSGGMAR RIALARAIAL DPQLIMYDEP FAGLDPISLG ITANLIRTLN HALGATSILV THDVPESFAI ADYVYFLANG GVLAEGTPDE LRASTDPSVR QFIDGAPDGP FKFHYMSQPL AADFGLGGGR R
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtga gctcctcctc cgagtccctg ctggcgcttc gcgacgtcga tttcggctac ggcgagcgtc tcgtcctgtc gaacctgaac atgcgcttcg cacgcggcca ggtggtcgcg gtgatgggcg gttcaggctg cggcaagacc accgtgctgc gcctgatcgg cgggctcgtg cgcgcacgcc gcggccaggt gctgttcgac ggcgccgatg tcggcgcgca gacgcgcgag ggcctctacg cgctgcgccg caagatgggc atgctgtttc agttcggcgc gctcttcacc gacatgtcgg tgtacgacaa cgtcgcgttc gcgctgcgcg agcacacgga tctgcccgaa gagctgatcc gcgatctcgt gctgatgaag ctcaacgcgg tcggcctgcg cggcgcgcgc gagctgatgc cgtccgaggt gtcgggcggg atggcgcggc gcatcgcgct cgcgcgggcg atcgcgctcg acccgcagct catcatgtac gacgagccgt tcgcgggcct cgaccctatt tcgctcggca tcaccgcaaa cctgatccgc acgctgaacc atgcgctcgg cgcgacgtcg atcctcgtca cgcacgacgt gccggaatcg ttcgcgatcg cggattacgt gtacttcctc gcgaacggcg gcgtgctggc cgagggcacg cccgacgagc tgcgtgcgtc gaccgatccg agcgtgcgcc agttcatcga cggcgcgccg gacggcccgt tcaaatttca ctacatgagc cagccgctcg cggcggactt cggacttggc ggagggcgca gatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat