SSGCID: Target Page

ButhA.01674.a

Effector protein BopA

CENTER ID:	ButhA.01674.a
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.01674.a.A1.GE34813	Full length( ButhA.01674.a )		1	512

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.958
RefSeq:	YP_439046.1
UniProt:	Q2T703

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MISVGAFAAC ARQGERVVVG DGARGPAVSV ARLGMKERLF AFLSHVPLLK HCDAVRRYAE QVRTENRRTL EVFVLALSKR YGPEGAKAAF DYGARRDGAP LDQRRVRNMV SIAEHFHGTG DAKPMARQIV FRSWECQGLD HPGHASLTIK NQADADAGRH VYEHVSWWPD RKLGGKENVN RVEPKMQDGY RIDKRSEIST TTEQRLREGD AARRKILADG FKYANEDERH DARFFPRAGQ KLDKDAQWGL SARKVYFPAI GFNHDKRDTD GPRAFVLFGL NEAAMLRDAR TVKEGAKTGE LKFQMISKKE NCASMALRVL RAGGAEHFVP FTAAWISEDP NRAHAYALAV QARIDALNQQ RADVERHCAR LRGSASVRQA WRAFSEAGNA SGSPLADQAG RGRASAHTRQ ARLDEHAREV ERIGASFAEL SAGRSGKHRD RADADLADAM KRCAPSARDD VAALTRKASV LVETLGRHLD APPPSDSSAL RMLAAHAMIG RIEAFMADAI AA

NT Sequence

ATGATCAGTG TCGGTGCATT CGCCGCGTGC GCGCGGCAGG GCGAGCGTGT CGTCGTCGGC GACGGCGCGC GCGGCCCGGC GGTGTCGGTC GCGAGGCTGG GCATGAAGGA GCGGTTGTTC GCGTTTCTCT CGCATGTCCC GCTGCTCAAG CACTGCGACG CGGTGCGGCG CTATGCGGAG CAGGTCCGCA CGGAAAACCG CCGCACGCTC GAGGTGTTCG TGCTCGCGCT ATCGAAGCGA TATGGCCCGG AAGGCGCGAA GGCGGCGTTC GACTACGGCG CGCGGCGCGA CGGCGCGCCG CTCGATCAGC GGCGCGTGCG CAACATGGTG TCGATCGCCG AGCACTTCCA CGGCACGGGC GACGCGAAGC CGATGGCCCG GCAAATCGTA TTTCGGTCGT GGGAATGCCA GGGCCTCGAT CATCCGGGGC ACGCGTCGCT GACGATCAAG AATCAGGCGG ATGCCGATGC CGGCCGGCAC GTGTACGAGC ACGTCAGCTG GTGGCCGGAT CGAAAACTGG GTGGCAAGGA GAATGTCAAT CGCGTCGAGC CGAAGATGCA GGACGGATAT CGGATCGACA AGCGCTCGGA GATTTCGACT ACGACGGAAC AGAGACTTCG TGAGGGCGAC GCGGCGAGGA GAAAGATCCT CGCGGACGGC TTCAAATATG CGAATGAGGA CGAGCGTCAC GACGCGCGGT TTTTCCCGAG AGCCGGCCAG AAGTTGGACA AGGATGCCCA ATGGGGGCTG AGCGCCCGCA AGGTCTATTT TCCGGCGATC GGATTCAACC ACGACAAGCG CGATACGGAT GGCCCGCGCG CGTTCGTGCT GTTCGGGCTG AACGAGGCGG CGATGCTGCG CGATGCACGC ACCGTGAAGG AAGGCGCGAA GACGGGCGAG CTCAAGTTTC AGATGATCAG CAAAAAGGAA AACTGCGCGT CGATGGCGCT GCGCGTGCTG CGCGCCGGCG GCGCCGAGCA CTTCGTGCCG TTTACGGCCG CATGGATCTC CGAGGATCCG AATCGCGCGC ACGCCTATGC GCTGGCCGTG CAGGCGAGGA TCGACGCGCT GAACCAGCAG CGCGCCGACG TCGAGCGCCA CTGCGCTCGG TTGCGCGGCA GCGCGTCGGT GCGGCAGGCG TGGCGCGCGT TCTCCGAGGC CGGCAATGCG TCGGGAAGCC CGCTCGCGGA CCAGGCCGGC CGCGGGCGAG CGTCGGCGCA CACGCGGCAA GCGCGGCTCG ACGAGCATGC GCGCGAGGTC GAGCGAATCG GCGCGTCCTT CGCCGAGCTG TCCGCCGGGC GATCCGGCAA GCATCGCGAT CGGGCGGACG CGGACCTGGC CGATGCGATG AAGCGTTGCG CGCCTTCAGC GCGCGACGAC GTCGCGGCGC TGACGCGCAA GGCGAGCGTG CTCGTCGAGA CGCTCGGCCG GCATCTCGAC GCGCCGCCGC CGAGCGATTC GTCCGCGCTG AGGATGCTGG CCGCGCACGC GATGATCGGC CGGATCGAGG CGTTCATGGC GGACGCGATC GCCGCC

Details for ButhA.01674.a.A1.GE34813

HARVESTED ON:	3/13/2012
SEQUENCED ON:	3/12/2012
EXPECTED MW:	58kDa
OBSERVED MW:	60kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	79
PERCENT COVERAGE:	50

Validated AA Sequence

Validated NT Sequence

attttnttnn ntttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgatca gtgtcggtgc attcgccgcg tgcgcgcggc agggcgagcg tgtcgtcgtc ggcgacggcg cgcgcggccc ggcggtgtcg gtcgcgaggc tgggcatgaa ggagcggttg ttcgcgtttc tctcgcatgt cccgctgctc aagcactgcg acgcggtgcg gcgctatgcg gagcaggtcc gcacggaaaa ccgccgcacg ctcgaggtgt tcgtgctcgc gctatcgaag cgatatggcc cggaaggcgc gaaggcggcg ttcgactacg gcgcgcggcg cgacggcgcg ccgctcgatc agcggcgcgt gcgcaacatg gtgtcgatcg ccgagcactt ccacggcacg ggcgacgcga agccgatggc ccggcaaatc gtatttcggt cgtgggaatg ccagggcctc gatcatccgg ggcacgcgtc gctgacgatc aagaatcang cggatgccga tgccggccgg cacgtgtacg agcacgtcag ctggtgnncg gatcgaaaac tgggtggcaa ggagaatgtc aatcgcgtcn agccgaanat gcnnncngat atcggatcga caagcgctcg ganatttcga ctacnacnga acagagactt cntgagggcg acgcggcgag gagaaagatc ctcgcggang gcttcnaata tgcgaatgag gacnagcgtc acgacncgcg gntttcccna gagccggnca gaagttggac nanntgccca agggggctga ncgcccncan gncnnntttn nnngatcgga ttcanccncn acnagcncga tannnngncn ngcnnntcnn gctgtnggnc tgaacnagng nnngcnncnn natgnnnnnn ncntnannag nnnnanannn nnanctcnag tttnnnnann nnncagcaaa nnnaanngnn ngtnnnnnnn ntnnncntgn nnnnnnnnnn nnnnnnnnnn nnttnngnnn nnngnnnnnc nnnnacnaan nnnnnnnnnn ctnnnnnc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMISVGAFAA CARQGERVVV GDGARGPAVS VARLGMKERL FAFLSHVPLL KHCDAVRRYA EQVRTENRRT LEVFVLALSK RYGPEGAKAA FDYGARRDGA PLDQRRVRNM VSIAEHFHGT GDAKPMARQI VFRSWECQGL DHPGHASLTI KNQADADAGR HVYEHVSWWP DRKLGGKENV NRVEPKMQDG YRIDKRSEIS TTTEQRLREG DAARRKILAD GFKYANEDER HDARFFPRAG QKLDKDAQWG LSARKVYFPA IGFNHDKRDT DGPRAFVLFG LNEAAMLRDA RTVKEGAKTG ELKFQMISKK ENCASMALRV LRAGGAEHFV PFTAAWISED PNRAHAYALA VQARIDALNQ QRADVERHCA RLRGSASVRQ AWRAFSEAGN ASGSPLADQA GRGRASAHTR QARLDEHARE VERIGASFAE LSAGRSGKHR DRADADLADA MKRCAPSARD DVAALTRKAS VLVETLGRHL DAPPPSDSSA LRMLAAHAMI GRIEAFMADA IAA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gatcagtgtc ggtgcattcg ccgcgtgcgc gcggcagggc gagcgtgtcg tcgtcggcga cggcgcgcgc ggcccggcgg tgtcggtcgc gaggctgggc atgaaggagc ggttgttcgc gtttctctcg catgtcccgc tgctcaagca ctgcgacgcg gtgcggcgct atgcggagca ggtccgcacg gaaaaccgcc gcacgctcga ggtgttcgtg ctcgcgctat cgaagcgata tggcccggaa ggcgcgaagg cggcgttcga ctacggcgcg cggcgcgacg gcgcgccgct cgatcagcgg cgcgtgcgca acatggtgtc gatcgccgag cacttccacg gcacgggcga cgcgaagccg atggcccggc aaatcgtatt tcggtcgtgg gaatgccagg gcctcgatca tccggggcac gcgtcgctga cgatcaagaa tcaggcggat gccgatgccg gccggcacgt gtacgagcac gtcagctggt ggccggatcg aaaactgggt ggcaaggaga atgtcaatcg cgtcgagccg aagatgcagg acggatatcg gatcgacaag cgctcggaga tttcgactac gacggaacag agacttcgtg agggcgacgc ggcgaggaga aagatcctcg cggacggctt caaatatgcg aatgaggacg agcgtcacga cgcgcggttt ttcccgagag ccggccagaa gttggacaag gatgcccaat gggggctgag cgcccgcaag gtctattttc cggcgatcgg attcaaccac gacaagcgcg atacggatgg cccgcgcgcg ttcgtgctgt tcgggctgaa cgaggcggcg atgctgcgcg atgcacgcac cgtgaaggaa ggcgcgaaga cgggcgagct caagtttcag atgatcagca aaaaggaaaa ctgcgcgtcg atggcgctgc gcgtgctgcg cgccggcggc gccgagcact tcgtgccgtt tacggccgca tggatctccg aggatccgaa tcgcgcgcac gcctatgcgc tggccgtgca ggcgaggatc gacgcgctga accagcagcg cgccgacgtc gagcgccact gcgctcggtt gcgcggcagc gcgtcggtgc ggcaggcgtg gcgcgcgttc tccgaggccg gcaatgcgtc gggaagcccg ctcgcggacc aggccggccg cgggcgagcg tcggcgcaca cgcggcaagc gcggctcgac gagcatgcgc gcgaggtcga gcgaatcggc gcgtccttcg ccgagctgtc cgccgggcga tccggcaagc atcgcgatcg ggcggacgcg gacctggccg atgcgatgaa gcgttgcgcg ccttcagcgc gcgacgacgt cgcggcgctg acgcgcaagg cgagcgtgct cgtcgagacg ctcggccggc atctcgacgc gccgccgccg agcgattcgt ccgcgctgag gatgctggcc gcgcacgcga tgatcggccg gatcgaggcg ttcatggcgg acgcgatcgc cgcctaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa