SSGCID: Target Page

ButhA.01673.g

Outer membrane efflux protein

CENTER ID:	ButhA.01673.g
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.01673.g.A2.GE34807	Mature protein( ButhA.01673.g )		27	463

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.01673.g.A2.PS01497	Mature protein( ButhA.01673.g )		27	463

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.1999
RefSeq:	YP_439987.1
UniProt:	Q2T4B2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTILPTHRFA RRAAALACAG ALLSACATPS RNAPPPAVEL PAQWAAPVAG QASATPDGWW RRFGDPTLDR LIDDALRTNN DLAAAAIRVY RAQLQAGLAD TNLTPNVTLG ATGNVSRTLD THRMGRTSGV TATLGYELDL WGKLAAQRDA ARWELEATQA DRDAARLSLI GTTAALYWQI AYLNQLIAIG DANIAYARRT LELVQSRHAA GATSGLDLAQ AERNLSALRA DQTQLIQQRT ENRNALAILF DRPPQRQAAE RDALPAAPLP DIAAGVPAEL LGRRPDLRAA EFRLRESLAN VDVTRTSFYP TFTLTGNVGT ASTSLERVLQ NPVATLGLGL ALPFIQWNTM QLQIKVSKSQ YEEAVVNFRQ SLYRALGEVE NALSARAQLE AEADERAHAL AQAQRAETLA KARFAAGATG VQPWLDEQQR LRDAQSALAR NQLGRLNNRM SLYKALGGED RPA

NT Sequence

ATGACGATTC TCCCGACTCA TCGTTTCGCC CGGCGCGCCG CCGCGCTCGC GTGCGCGGGC GCGCTGCTGT CCGCCTGCGC GACGCCGTCG CGCAACGCGC CGCCGCCCGC GGTCGAGCTG CCCGCGCAAT GGGCCGCGCC CGTCGCGGGG CAGGCGAGCG CCACGCCCGA CGGCTGGTGG CGCCGCTTCG GCGACCCGAC GCTCGATCGC CTGATCGACG ACGCGCTGCG CACGAACAAC GATCTCGCGG CCGCCGCGAT CCGCGTCTAT CGCGCGCAAT TGCAGGCCGG GCTCGCGGAC ACGAACCTGA CGCCGAACGT GACGCTCGGC GCGACGGGCA ACGTATCGCG CACGCTCGAC ACGCACCGGA TGGGCCGCAC GAGCGGCGTC ACGGCTACGC TCGGCTACGA GCTCGACCTG TGGGGCAAGC TTGCCGCGCA GCGCGACGCC GCGCGCTGGG AACTCGAAGC AACGCAGGCC GACCGCGATG CGGCGCGGCT CTCGCTGATC GGCACGACGG CGGCACTGTA CTGGCAGATC GCCTATCTGA ATCAACTGAT CGCGATCGGC GATGCGAACA TCGCGTACGC GCGGCGCACG CTCGAGCTCG TGCAGTCGCG TCACGCGGCG GGCGCGACGT CCGGGCTCGA CCTCGCGCAA GCCGAGCGCA ACCTGTCCGC GCTGCGCGCG GACCAGACGC AACTGATCCA GCAACGCACC GAGAACCGCA ACGCGCTTGC GATCCTGTTC GACCGTCCGC CGCAACGGCA AGCGGCCGAG CGCGACGCAC TGCCCGCGGC GCCGCTTCCG GACATCGCCG CAGGCGTGCC GGCCGAGCTG CTCGGCCGCC GGCCCGATTT GCGCGCGGCC GAGTTCCGGC TGCGCGAATC GCTCGCGAAC GTCGACGTGA CGCGCACGAG CTTCTATCCG ACATTCACGC TGACGGGCAA CGTCGGCACC GCGAGCACGA GCCTCGAGCG CGTGCTGCAG AATCCCGTCG CGACGCTCGG CCTCGGGCTC GCGCTGCCGT TCATCCAGTG GAACACGATG CAGTTGCAGA TCAAGGTGTC GAAATCGCAG TACGAGGAAG CCGTCGTCAA TTTCCGGCAG AGCCTGTATC GCGCGCTCGG CGAAGTCGAG AACGCGCTGT CGGCGCGTGC GCAGCTCGAG GCCGAGGCCG ACGAGCGCGC GCACGCGCTC GCGCAGGCGC AGCGCGCCGA AACGCTCGCG AAGGCGCGCT TCGCGGCCGG CGCGACGGGC GTGCAGCCAT GGCTCGACGA ACAGCAACGG CTGCGCGATG CGCAAAGCGC GCTCGCGCGC AATCAATTGG GACGGCTCAA CAACCGGATG AGCCTGTACA AGGCGCTGGG CGGCGAGGAC CGCCCCGCC

Details for ButhA.01673.g.A2.GE34807

HARVESTED ON:	3/13/2012
SEQUENCED ON:	3/12/2012
EXPECTED MW:	49kDa
OBSERVED MW:	49kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	94

Validated AA Sequence

MATPSRNAPP PAVELPAQWA APVAGQASAT PDGWWRRFGD PTLDRLIDDA LRTNNDLAAA AIRVYRAQLQ AGLADTNLTP NVTLGATGNV SRTLDTHRMG RTSGVTATLG YELDLWGKLA AQRDAARWEL EATQADRDAA RLSLIGTTAA LYWQIAYLNQ LIAIGDANIA YARRTLELVQ SRHAAGATSG LDLAQAERNL SALRADQTQL IQQRTENRNA LAILFDRPPQ RQAAERDALP AAPLPDIAAG VPAELLGRRP DLRAAEFRLR ESLANVDVTR TSFYPTFTLT GNVGTASTSL ERVLQNPVAT LGLGLALPFI QWNTMQLQIK VSKSQYEEAV VNFRQSLYRA LGEVENALSA RAQLEAEADE RAHALAQAQR AETLAKARFA AGATGVQPWL DEQQRLRDAQ SALARNQLGR LNNRMSLYKA LGGE

Validated NT Sequence

cctcgccgcc cagcgccttg tacaggctca tccggttgtt gagccgtccc aattgattgc gcgcgagcgc gctttgcgca tcgcgcagcc gttgctgttc gtcgagccat ggctgcacgc ccgtcgcgcc ggccgcgaag cgcgccttcg cgagcgtttc ggcgcgctgc gcctgcgcga gcgcgtgcgc gcgctcgtcg gcctcggcct cgagctgcgc acgcgccgac agcgcgttct cgacttcgcc gagcgcgcga tacaggctct gccggaaatt gacgacggct tcctcgtact gcgatttcga caccttgatc tgcaactgca tcgtgttcca ctggatgaac ggcagcgcga gcccgaggcc gagcgtcgcg acgggattct gcagcacgcg ctcgaggctc gtgctcgcgg tgccgacgtt gcccgtcagc gtgaatgtcg gatagaagct cgtgcgcgtc acgtcgacgt tcgcgagcga ttcgcgcagc cggaactcgg ccgcgcgcaa atcgggccgg cggccgagca gctcggccgg cacgcctgcg gcgatgtccg gaagcggcgc cgcgggcagt gcgtcgcgct cggccgcttg ccgttgcggc ggacggtcga acaggatcgc aagcgcgttg cggttctcgg tgcgttgctg gatcagttgc gtctggtccg cgcgcagcgc ggacaggttg cgctcggctt gcgcgaggtc gagcccggac gtcgcgcccg ccgcgtgacg cgactgcacg agctcgagcg tgcgccgcgc gtacgcgatg ttcgcatcgc cgatcgcgat cagttgattc agataggcga tctgccagta cagtgccgcc gtcgtgccga tcagcgagag ccgcgccgca tcgcggtcgg cctgcgttgc ttcgagttcc cagcgcgcgg cgtcgcgctg cgcggcaagc ttgccccaca ggtcgagctc gtagccgagc gtagccgtga cgccgctcgt gcggcccatc cggtgcgtgt cgagcgtgcg cgatacgttg cccgtcgcgc cgagcgtcac gttcggcgtc aggttcgtgt ccgcgagccc ggcctgcaat tgcgcgcgat agacgcggat cgcggcggcc gcgagatcgt tgttcgtgcg cagcgcgtcg tcgatcaggc gatcgagcgt cgggtcgccg aagcggcgcc accagccgtc gggcgtggcg ctcgcctgcc ccgcgacggg cgcggcccat tgcgcgggca gctcgaccgc gggcggcggc gcgttgcgcg acggcgtcgc catcgaacca ggaccctggg tctgagcttc ca

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMATPSRNAP PPAVELPAQW AAPVAGQASA TPDGWWRRFG DPTLDRLIDD ALRTNNDLAA AAIRVYRAQL QAGLADTNLT PNVTLGATGN VSRTLDTHRM GRTSGVTATL GYELDLWGKL AAQRDAARWE LEATQADRDA ARLSLIGTTA ALYWQIAYLN QLIAIGDANI AYARRTLELV QSRHAAGATS GLDLAQAERN LSALRADQTQ LIQQRTENRN ALAILFDRPP QRQAAERDAL PAAPLPDIAA GVPAELLGRR PDLRAAEFRL RESLANVDVT RTSFYPTFTL TGNVGTASTS LERVLQNPVA TLGLGLALPF IQWNTMQLQI KVSKSQYEEA VVNFRQSLYR ALGEVENALS ARAQLEAEAD ERAHALAQAQ RAETLAKARF AAGATGVQPW LDEQQRLRDA QSALARNQLG RLNNRMSLYK ALGGEDRPA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgacgccg tcgcgcaacg cgccgccgcc cgcggtcgag ctgcccgcgc aatgggccgc gcccgtcgcg gggcaggcga gcgccacgcc cgacggctgg tggcgccgct tcggcgaccc gacgctcgat cgcctgatcg acgacgcgct gcgcacgaac aacgatctcg cggccgccgc gatccgcgtc tatcgcgcgc aattgcaggc cgggctcgcg gacacgaacc tgacgccgaa cgtgacgctc ggcgcgacgg gcaacgtatc gcgcacgctc gacacgcacc ggatgggccg cacgagcggc gtcacggcta cgctcggcta cgagctcgac ctgtggggca agcttgccgc gcagcgcgac gccgcgcgct gggaactcga agcaacgcag gccgaccgcg atgcggcgcg gctctcgctg atcggcacga cggcggcact gtactggcag atcgcctatc tgaatcaact gatcgcgatc ggcgatgcga acatcgcgta cgcgcggcgc acgctcgagc tcgtgcagtc gcgtcacgcg gcgggcgcga cgtccgggct cgacctcgcg caagccgagc gcaacctgtc cgcgctgcgc gcggaccaga cgcaactgat ccagcaacgc accgagaacc gcaacgcgct tgcgatcctg ttcgaccgtc cgccgcaacg gcaagcggcc gagcgcgacg cactgcccgc ggcgccgctt ccggacatcg ccgcaggcgt gccggccgag ctgctcggcc gccggcccga tttgcgcgcg gccgagttcc ggctgcgcga atcgctcgcg aacgtcgacg tgacgcgcac gagcttctat ccgacattca cgctgacggg caacgtcggc accgcgagca cgagcctcga gcgcgtgctg cagaatcccg tcgcgacgct cggcctcggg ctcgcgctgc cgttcatcca gtggaacacg atgcagttgc agatcaaggt gtcgaaatcg cagtacgagg aagccgtcgt caatttccgg cagagcctgt atcgcgcgct cggcgaagtc gagaacgcgc tgtcggcgcg tgcgcagctc gaggccgagg ccgacgagcg cgcgcacgcg ctcgcgcagg cgcagcgcgc cgaaacgctc gcgaaggcgc gcttcgcggc cggcgcgacg ggcgtgcagc catggctcga cgaacagcaa cggctgcgcg atgcgcaaag cgcgctcgcg cgcaatcaat tgggacggct caacaaccgg atgagcctgt acaaggcgct gggcggcgag gaccgccccg cctaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa

Details for ButhA.01673.g.A2.PS01497

PURIFICATION DATe:	5/15/2012
CONCENTRATION:	8.22mg/ml
OBSERVED MW:	47kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	9
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	94

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCGACGCCG TCGCGCAACG CGCCGCCGCC CGCGGTCGAG CTGCCCGCGC AATGGGCCGC GCCCGTCGCG GGGCAGGCGA GCGCCACGCC CGACGGCTGG TGGCGCCGCT TCGGCGACCC GACGCTCGAT CGCCTGATCG ACGACGCGCT GCGCACGAAC AACGATCTCG CGGCCGCCGC GATCCGCGTC TATCGCGCGC AATTGCAGGC CGGGCTCGCG GACACGAACC TGACGCCGAA CGTGACGCTC GGCGCGACGG GCAACGTATC GCGCACGCTC GACACGCACC GGATGGGCCG CACGAGCGGC GTCACGGCTA CGCTCGGCTA CGAGCTCGAC CTGTGGGGCA AGCTTGCCGC GCAGCGCGAC GCCGCGCGCT GGGAACTCGA AGCAACGCAG GCCGACCGCG ATGCGGCGCG GCTCTCGCTG ATCGGCACGA CGGCGGCACT GTACTGGCAG ATCGCCTATC TGAATCAACT GATCGCGATC GGCGATGCGA ACATCGCGTA CGCGCGGCGC ACGCTCGAGC TCGTGCAGTC GCGTCACGCG GCGGGCGCGA CGTCCGGGCT CGACCTCGCG CAAGCCGAGC GCAACCTGTC CGCGCTGCGC GCGGACCAGA CGCAACTGAT CCAGCAACGC ACCGAGAACC GCAACGCGCT TGCGATCCTG TTCGACCGTC CGCCGCAACG GCAAGCGGCC GAGCGCGACG CACTGCCCGC GGCGCCGCTT CCGGACATCG CCGCAGGCGT GCCGGCCGAG CTGCTCGGCC GCCGGCCCGA TTTGCGCGCG GCCGAGTTCC GGCTGCGCGA ATCGCTCGCG AACGTCGACG TGACGCGCAC GAGCTTCTAT CCGACATTCA CGCTGACGGG CAACGTCGGC ACCGCGAGCA CGAGCCTCGA GCGCGTGCTG CAGAATCCCG TCGCGACGCT CGGCCTCGGG CTCGCGCTGC CGTTCATCCA GTGGAACACG ATGCAGTTGC AGATCAAGGT GTCGAAATCG CAGTACGAGG AAGCCGTCGT CAATTTCCGG CAGAGCCTGT ATCGCGCGCT CGGCGAAGTC GAGAACGCGC TGTCGGCGCG TGCGCAGCTC GAGGCCGAGG CCGACGAGCG CGCGCACGCG CTCGCGCAGG CGCAGCGCGC CGAAACGCTC GCGAAGGCGC GCTTCGCGGC CGGCGCGACG GGCGTGCAGC CATGGCTCGA CGAACAGCAA CGGCTGCGCG ATGCGCAAAG CGCGCTCGCG CGCAATCAAT TGGGACGGCT CAACAACCGG ATGAGCCTGT ACAAGGCGCT GGGCGGCGAG GACCGCCCCG CCTAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA