ButhA.01669.a

Sensory box histidine kinase, putative

CENTER ID: ButhA.01669.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.01669.a.A1.GE34805 Full length( ButhA.01669.a ) 1 433
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.366
RefSeq: YP_438525.1
UniProt: Q2T8H4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTRPGPKYPR EVGVGAMDRS MTDGPQVYEW CYRSRTGVDM LSEATATYVR LRERDVVMVQ FRDISAEEAT RQALRRYEAR LREFMQDLDE GVAVLTPHGG VQFISESGRR VLGIAVGEPL GGVLDYCTVA DRDRLIAQLR DAPACRPSEP QRYRIARRDG ASRWVRITCR RVDIEGDLSG LLIHFRDVTD EVALEEARRA EARMLEYAGR YNAMGEMAAA IAHELSQPLA AVRNFIEGAI RRLGQPGATD DIVWGLRSAD RQAEHAALII KSVREYIVRR ESVETVVDLR DILADVSYFI DLRAKEAGVA VTIMQADAPL PVRCERVLIG QVILNLAFNA IEALAGIERP ARTLTLRTGG HAAHAVLYAV DNGPGVPDGM HAQLFDGFSS SKVGGNGIGL SLCKNIVTRH GGQIRAQPAA GGGLECRFTL PLA
NT Sequence
ATGACGCGTC CCGGGCCGAA GTACCCGCGC GAGGTTGGCG TCGGCGCGAT GGACCGCTCG ATGACCGATG GTCCGCAGGT GTACGAGTGG TGTTACCGGT CGCGCACCGG CGTCGACATG CTGTCGGAGG CCACCGCGAC ATACGTGCGG TTGCGCGAGC GCGACGTCGT GATGGTGCAG TTTCGCGACA TCAGCGCGGA GGAGGCGACT CGCCAGGCGC TGCGGCGCTA CGAGGCGAGG CTGCGGGAGT TCATGCAGGA CCTGGACGAA GGCGTCGCCG TGCTCACGCC GCACGGCGGC GTGCAGTTCA TCAGCGAATC GGGGCGGCGC GTGCTCGGCA TCGCGGTGGG CGAGCCGCTT GGCGGCGTGC TCGATTACTG CACGGTCGCC GATCGCGACC GGCTGATCGC GCAACTGCGC GATGCGCCCG CGTGCCGTCC GTCCGAGCCG CAGCGCTACC GGATCGCGCG CCGCGACGGC GCGTCGCGGT GGGTGCGGAT CACGTGCCGG CGCGTCGACA TCGAAGGCGA CCTGAGCGGC TTGCTGATCC ACTTCCGCGA CGTGACCGAC GAAGTGGCGC TGGAGGAAGC GCGGCGCGCC GAGGCGCGGA TGCTCGAATA CGCGGGGCGC TACAACGCGA TGGGCGAGAT GGCGGCCGCG ATCGCGCACG AGCTCAGCCA GCCGCTCGCG GCGGTGCGCA ATTTCATCGA GGGCGCGATC CGGCGGCTCG GCCAGCCGGG CGCGACGGAC GACATCGTCT GGGGGCTGCG CAGCGCCGAC CGGCAGGCGG AGCACGCGGC GCTCATCATC AAGAGCGTGC GCGAATACAT CGTGCGGCGC GAGTCCGTCG AGACGGTCGT CGATCTGCGT GACATCCTGG CGGACGTGTC GTACTTCATC GATCTGCGCG CGAAGGAAGC GGGCGTTGCG GTGACGATCA TGCAGGCGGA CGCGCCGCTG CCGGTGCGCT GCGAGCGGGT GCTGATCGGG CAGGTCATCC TGAATCTCGC ATTCAATGCG ATCGAGGCGC TGGCCGGCAT CGAGCGGCCG GCTCGCACGT TGACGCTCCG CACCGGCGGG CATGCGGCGC ATGCGGTGCT GTATGCCGTC GACAACGGGC CCGGCGTTCC CGACGGCATG CATGCGCAAC TGTTCGACGG GTTTTCGTCG TCGAAAGTCG GCGGCAACGG GATCGGGCTG TCGCTGTGCA AGAACATCGT CACGCGCCAC GGCGGCCAGA TCAGGGCGCA GCCGGCGGCG GGCGGCGGGC TCGAATGCCG CTTCACGCTG CCGCTCGCA
Details for ButhA.01669.a.A1.GE34805
HARVESTED ON: 3/13/2012
SEQUENCED ON: 3/12/2012
EXPECTED MW: 49kDa
OBSERVED MW: 48kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 79
PERCENT COVERAGE: 76
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTRPGPKYP REVGVGAMDR SMTDGPQVYE WCYRSRTGVD MLSEATATYV RLRERDVVMV QFRDISAEEA TRQALRRYEA RLREFMQDLD EGVAVLTPHG GVQFISESGR RVLGIAVGEP LGGVLDYCTV ADRDRLIAQL RDAPACRPSE PQRYRIARRD GASRWVRITX RRVXIEXDLS GLLIHFRDVT DEXALEEARR AXARMLEYAG RYXAMGEMAA AIAHXLSQPL AAVRNFIEGA IRRLGQPGAX XDIVWGXXSX XRXXXTXXSS SXRAXXIXXR ESSRXXXXXX XXXXXRXSSI XXXXXXXXTI XXXXXXXXXX XXXXXXXX
Validated NT Sequence
ttntttactt taagaaggag atataccatg gctcatcacc atcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atgacgcgtc ccgggccgaa gtacccgcgc gaggttggcg tcggcgcgat ggaccgctcg atgaccgatg gtccgcaggt gtacgagtgg tgttaccggt cgcgcaccgg cgtcgacatg ctgtcggagg ccaccgcgac atacgtgcgg ttgcgcgagc gcgacgtcgt gatggtgcag tttcgcgaca tcagcgcgga ggaggcgact cgccaggcgc tgcggcgcta cgaggcgagg ctgcgggagt tcatgcagga cctggacgaa ggcgtcgccg tgctcacgcc gcacggcggc gtgcagttca tcagcgaatc ggggcggcgc gtgctcggca tcgcggtggg cgagccgctt ggcggcgtgc tcgattactg cacggtcgcc gatcgcgacc ggctgatcgc gcaactgcgc gatgcgcccg cgtgccgtcc gtccgagccg cagcgctacc ggatcgcgcg ccgcgacggc gcgtcgcggt gggtgcggat cacgtgncgg cgcgtcnaca tcgaangcga cctgagcggc ttgctgatcc acttccgcga cgtgaccgac gaantggcgc tggaggaagc gcggcgcgcc naggcgcgga tgctcgaata cgcggggcgc tacnacgcga tgggcgagat ggcggccgcg atcgcgcacg anctcagcca gccnctcgcg gcggtgcgca atttcatcga gggcgcgatc cggcggctcg gncagccggg cgcgangnac gacatcgtct gggggntgcn cagcgncnan cggcnnnnga ncacgngnng ctcatcatca nancgtgcgc nanacatcnn gnngcgcgag tcntcnagan gtcntcnanc tnnnngnnnt cnngnnnnnn nncgtanttc atcgatcnnn nnncnananc ngnnnnnnnn nacnatcntg nnnnnnannn nnntnnnnnn nnnnnnnngn nntnatnnnn ngnnnnnnnn nana
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTRPGPKYP REVGVGAMDR SMTDGPQVYE WCYRSRTGVD MLSEATATYV RLRERDVVMV QFRDISAEEA TRQALRRYEA RLREFMQDLD EGVAVLTPHG GVQFISESGR RVLGIAVGEP LGGVLDYCTV ADRDRLIAQL RDAPACRPSE PQRYRIARRD GASRWVRITC RRVDIEGDLS GLLIHFRDVT DEVALEEARR AEARMLEYAG RYNAMGEMAA AIAHELSQPL AAVRNFIEGA IRRLGQPGAT DDIVWGLRSA DRQAEHAALI IKSVREYIVR RESVETVVDL RDILADVSYF IDLRAKEAGV AVTIMQADAP LPVRCERVLI GQVILNLAFN AIEALAGIER PARTLTLRTG GHAAHAVLYA VDNGPGVPDG MHAQLFDGFS SSKVGGNGIG LSLCKNIVTR HGGQIRAQPA AGGGLECRFT LPLA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacgcgtccc gggccgaagt acccgcgcga ggttggcgtc ggcgcgatgg accgctcgat gaccgatggt ccgcaggtgt acgagtggtg ttaccggtcg cgcaccggcg tcgacatgct gtcggaggcc accgcgacat acgtgcggtt gcgcgagcgc gacgtcgtga tggtgcagtt tcgcgacatc agcgcggagg aggcgactcg ccaggcgctg cggcgctacg aggcgaggct gcgggagttc atgcaggacc tggacgaagg cgtcgccgtg ctcacgccgc acggcggcgt gcagttcatc agcgaatcgg ggcggcgcgt gctcggcatc gcggtgggcg agccgcttgg cggcgtgctc gattactgca cggtcgccga tcgcgaccgg ctgatcgcgc aactgcgcga tgcgcccgcg tgccgtccgt ccgagccgca gcgctaccgg atcgcgcgcc gcgacggcgc gtcgcggtgg gtgcggatca cgtgccggcg cgtcgacatc gaaggcgacc tgagcggctt gctgatccac ttccgcgacg tgaccgacga agtggcgctg gaggaagcgc ggcgcgccga ggcgcggatg ctcgaatacg cggggcgcta caacgcgatg ggcgagatgg cggccgcgat cgcgcacgag ctcagccagc cgctcgcggc ggtgcgcaat ttcatcgagg gcgcgatccg gcggctcggc cagccgggcg cgacggacga catcgtctgg gggctgcgca gcgccgaccg gcaggcggag cacgcggcgc tcatcatcaa gagcgtgcgc gaatacatcg tgcggcgcga gtccgtcgag acggtcgtcg atctgcgtga catcctggcg gacgtgtcgt acttcatcga tctgcgcgcg aaggaagcgg gcgttgcggt gacgatcatg caggcggacg cgccgctgcc ggtgcgctgc gagcgggtgc tgatcgggca ggtcatcctg aatctcgcat tcaatgcgat cgaggcgctg gccggcatcg agcggccggc tcgcacgttg acgctccgca ccggcgggca tgcggcgcat gcggtgctgt atgccgtcga caacgggccc ggcgttcccg acggcatgca tgcgcaactg ttcgacgggt tttcgtcgtc gaaagtcggc ggcaacggga tcgggctgtc gctgtgcaag aacatcgtca cgcgccacgg cggccagatc agggcgcagc cggcggcggg cggcgggctc gaatgccgct tcacgctgcc gctcgcataa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa