ButhA.01643.a

UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase (BTH_I1113)

CENTER ID: ButhA.01643.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.01643.a.A1.GE32823 full length 1 514
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.01643.a.A1.PW33427 full length 1 514

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.3869
RefSeq: YP_441661.1
UniProt: Q2SZI8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSAARSSHPA HQQIAAALAW LRAHVASGAQ LHADTRSLQA GDVFVAYAVD GADNRPFIAD ALARGAAAVL YQPEGFPAAG LDPATSLAVP ALDALAGDIA SGWYGDPSDA MFAVGVTGTN GKTSCTQWIA AALTALREPC AVIGTLGSGM PGQLVHTGFT TPDAPQLQRS LAQLRDAGAQ AVAMEVSSHA LHQGRVNGTA FDVAVFTNLT QDHLDYHRTF DAYEAAKAKL FAWRGLHCAV VNRDDPAGRR LLANLVGRVR TIAYGIDAPA QGADADRELV ATNVRATATG TAFRVTSPWG EADVEVGTLG TFNVSNLLAV LGTLLAADVP FDAALAEIER LESVNGRMQR LGGRLQNDEP LVVVDYAHTP DALEKTLEAL RPIAQARGGE LVCMFGCGGD RDATKRPLMG AIAERLADKV VVTSDNPRSE EPQKIIDQIV AGMRDAANAR RVEDRASAIL QAVRGAARED VVLLAGKGHE ATQEIMGKKR AFSDQDHARL ALAARTTQTR GGGE
NT Sequence
atgagcgccg cgcgcagttc ccatccggcg caccagcaga tcgcggccgc gcttgcgtgg ctgcgcgcgc acgtcgcgtc cggcgcgcaa ttgcacgccg acacgcgcag cctgcaggcg ggcgacgtgt tcgtcgccta tgcggtcgac ggcgcggata atcgtccgtt cattgccgac gcgctcgcgc gcggcgcggc cgccgtgctg tatcagcccg aaggtttccc ggccgccggc ctcgacccgg cgacgtcgct cgccgtgccc gcgctcgacg cgctcgcggg cgatatcgcg agcggctggt acggcgatcc gagcgacgcg atgttcgcgg tcggcgtgac gggcacgaac ggcaagacgt cgtgcacgca atggatcgcg gccgcgctca cagcgttgcg cgagccgtgc gcggtgatcg gcacgctcgg cagcggaatg cccggccagc tcgtccacac gggcttcacg acgcccgatg cgccgcaact gcagcgcagc ctcgcgcaac tgcgcgacgc gggcgctcag gcggtcgcga tggaagtctc gtcgcatgcg ctgcatcagg ggcgcgtgaa cggcaccgcg ttcgacgtcg cggtgttcac gaacctcacg caggatcacc tcgattacca ccgcacgttc gacgcctacg aggccgcgaa ggcaaagctc ttcgcctggc gcgggctgca ttgcgcggtt gtcaatcgcg acgatccggc cggccgccgc ctgctcgcga atctcgtcgg ccgcgtgagg acgatcgcgt acggcatcga tgcgccggcg caaggcgccg acgccgatcg cgagctcgtc gcgacgaacg tccgcgcgac cgcgacgggc accgcgtttc gcgtgacttc gccgtggggc gaagcggacg tcgaggtcgg cacgctcggc acgttcaacg tgagcaatct gctcgcggtg ctcggcacgc tgctcgcggc cgacgtgccg ttcgacgcgg cgctcgccga aatcgagcgg ctcgaatcgg tcaacggccg gatgcagcgg ctcggcggcc ggctgcagaa cgacgagccg ctcgtcgtcg tcgattacgc gcacacgccg gacgcgctcg agaagacgct cgaagcgctg cgcccgatcg cgcaggcgcg cggcggcgag ctcgtctgca tgttcggctg cggcggcgac cgcgacgcga cgaagcgccc gctgatgggc gcgatcgccg agcggctcgc ggacaaggtc gtcgtgacga gcgacaaccc gcgcagcgag gagccgcaga agatcatcga tcagatcgtc gccggcatgc gggacgccgc gaacgcgcgc cgagtcgagg accgcgcgag cgcgatcctg caggccgtgc gcggcgccgc gcgcgaggac gtcgtgctgc tcgccggcaa ggggcacgag gcgacgcagg agatcatggg caagaagcgt gcgttctccg atcaggatca cgcgcgcctc gcgctcgccg cgcgcacgac gcagactcgc ggaggcggcg aatga
Details for ButhA.01643.a.A1.GE32823
HARVESTED ON: 6/28/2011
SEQUENCED ON: 7/22/2011
EXPECTED MW: 56kDa
OBSERVED MW: 56kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 62
PERCENT COVERAGE: 96
Validated AA Sequence
MSAARSSHPA HQQIAAALAW LRAHVASGAQ LHADTRSLQA GDVFVAYAVD GADNRPFIAD ALARGAAAVL YQPEGFPAAG LDPATSLAVP ALDALAGDIA SGWYGDPSDA MFAVGVTGTN GKTSCTQWIA AALTALREPC AVIGTLGSGM PGQLVHTGFT TPDAPQLQRS LAQLRDAGAQ AVAMEVSSHA LHQGRVNGTA FDVAVFTNLT QDHLDYHRTF DAYEAAKAKL FAWRGLHCAV VNRDDPAXPP PARESRRPRE DDRVRHRCAG ARRRRRSRAR RDERPRDRDG HRVSRDFAVG RSGRRGRHAR HVQREQSARG ARHAARGRRA VRRGARRNRA ARIGQRPDAA ARRPAAERRA ARRRRLRAHA GRAREDARSA APDRAGARRR ARLHVRLRRR PRRDEAPADG RDRRAARGQG RRDERQPAQR GAAEDHRSDR RRHAGRRERA PSRGPRERDP AGRARRRARG RRAARRQGAR GDAGDHGQEA CVLRSGSRAP RARRAHDADS RRRR
Validated NT Sequence
tattcgccgc ctccgcgagt ctgcgtcgtg cgcgcggcga gcgcgaggcg cgcgtgatcc tgatcggaga acgcacgctt cttgcccatg atctcctgcg tcgcctcgtg ccccttgccg gcgagcagca cgacgtcctc gcgcgcggcg ccgcgcacgg cctgcaggat cgcgctcgcg cggtcctcga ctcggcgcgc gttcgcggcg tcccgcatgc cggcgacgat ctgatcgatg atcttctgcg gctcctcgct gcgcgggttg tcgctcgtca cgacgacctt gtccgcgagc cgctcggcga tcgcgcccat cagcgggcgc ttcgtcgcgt cgcggtcgcc gccgcagccg aacatgcaga cgagctcgcc gccgcgcgcc tgcgcgatcg ggcgcagcgc ttcgagcgtc ttctcgagcg cgtccggcgt gtgcgcgtaa tcgacgacga cgagcggctc gtcgttctgc agccggccgc cgagccgctg catccggccg ttgaccgatt cgagccgctc gatttcggcg agcgccgcgt cgaacggcac gtcggccgcg agcagcgtgc cgagcaccgc gagcagattg ctcacgttga acgtgccgag cgtgccgacc tcgacgtccg cttcgcccca cggcgaagtc acgcgaaacg cggtgcccgt cgcggtcgcg cggacgttcg tcgcgacgag ctcgcgatcg gcgtcggcgc cttgcgccgg cgcatcgatg ccgtacgcga tcgtcctcac gcggccgacg agattcgcga gcaggcggcg gccnggccgg atcgtcgcga ttgacaaccg cgcaatgcag cccgcgccag gcgaagagct ttgccttcgc ggcctcgtag gcgtcgaacg tgcggtggta atcgaggtga tcctgcgtga ggttcgtgaa caccgcgacg tcgaacgcgg tgccgttcac gcgcccctga tgcagcgcat gcgacgagac ttccatcgcg accgcctgag cgcccgcgtc gcgcagttgc gcgaggctgc gctgcagttg cggcgcatcg ggcgtcgtga agcccgtgtg gacgagctgg ccgggcattc cgctgccgag cgtgccgatc accgcgcacg gctcgcgcaa cgctgtgagc gcggccgcga tccattgcgt gcacgacgtc ttgccgttcg tgcccgtcac gccgaccgcg aacatcgcgt cgctcggatc gccgtaccag ccgctcgcga tatcgcccgc gagcgcgtcg agcgcgggca cggcgagcga cgtcgccggg tcgaggccgg cggccgggaa accttcgggc tgatacagca cggcggccgc gccgcgcgcg agcgcgtcgg caatgaacgg acgattatcc gcgccgtcga ccgcataggc gacgaacacg tcgcccgcct gcaggctgcg cgtgtcggcg tgcaattgcg cgccggacgc gacgtgcgcg cgcagccacg caagcgcggc cgcgatctgc tggtgcgccg gatgggaact gcgcgcggcg ctcatcgaac caggaccctg ggtctgagct tccaggg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSAARSSHP AHQQIAAALA WLRAHVASGA QLHADTRSLQ AGDVFVAYAV DGADNRPFIA DALARGAAAV LYQPEGFPAA GLDPATSLAV PALDALAGDI ASGWYGDPSD AMFAVGVTGT NGKTSCTQWI AAALTALREP CAVIGTLGSG MPGQLVHTGF TTPDAPQLQR SLAQLRDAGA QAVAMEVSSH ALHQGRVNGT AFDVAVFTNL TQDHLDYHRT FDAYEAAKAK LFAWRGLHCA VVNRDDPAGR RLLANLVGRV RTIAYGIDAP AQGADADREL VATNVRATAT GTAFRVTSPW GEADVEVGTL GTFNVSNLLA VLGTLLAADV PFDAALAEIE RLESVNGRMQ RLGGRLQNDE PLVVVDYAHT PDALEKTLEA LRPIAQARGG ELVCMFGCGG DRDATKRPLM GAIAERLADK VVVTSDNPRS EEPQKIIDQI VAGMRDAANA RRVEDRASAI LQAVRGAARE DVVLLAGKGH EATQEIMGKK RAFSDQDHAR LALAARTTQT RGGGE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcgccgcg cgcagttccc atccggcgca ccagcagatc gcggccgcgc ttgcgtggct gcgcgcgcac gtcgcgtccg gcgcgcaatt gcacgccgac acgcgcagcc tgcaggcggg cgacgtgttc gtcgcctatg cggtcgacgg cgcggataat cgtccgttca ttgccgacgc gctcgcgcgc ggcgcggccg ccgtgctgta tcagcccgaa ggtttcccgg ccgccggcct cgacccggcg acgtcgctcg ccgtgcccgc gctcgacgcg ctcgcgggcg atatcgcgag cggctggtac ggcgatccga gcgacgcgat gttcgcggtc ggcgtgacgg gcacgaacgg caagacgtcg tgcacgcaat ggatcgcggc cgcgctcaca gcgttgcgcg agccgtgcgc ggtgatcggc acgctcggca gcggaatgcc cggccagctc gtccacacgg gcttcacgac gcccgatgcg ccgcaactgc agcgcagcct cgcgcaactg cgcgacgcgg gcgctcaggc ggtcgcgatg gaagtctcgt cgcatgcgct gcatcagggg cgcgtgaacg gcaccgcgtt cgacgtcgcg gtgttcacga acctcacgca ggatcacctc gattaccacc gcacgttcga cgcctacgag gccgcgaagg caaagctctt cgcctggcgc gggctgcatt gcgcggttgt caatcgcgac gatccggccg gccgccgcct gctcgcgaat ctcgtcggcc gcgtgaggac gatcgcgtac ggcatcgatg cgccggcgca aggcgccgac gccgatcgcg agctcgtcgc gacgaacgtc cgcgcgaccg cgacgggcac cgcgtttcgc gtgacttcgc cgtggggcga agcggacgtc gaggtcggca cgctcggcac gttcaacgtg agcaatctgc tcgcggtgct cggcacgctg ctcgcggccg acgtgccgtt cgacgcggcg ctcgccgaaa tcgagcggct cgaatcggtc aacggccgga tgcagcggct cggcggccgg ctgcagaacg acgagccgct cgtcgtcgtc gattacgcgc acacgccgga cgcgctcgag aagacgctcg aagcgctgcg cccgatcgcg caggcgcgcg gcggcgagct cgtctgcatg ttcggctgcg gcggcgaccg cgacgcgacg aagcgcccgc tgatgggcgc gatcgccgag cggctcgcgg acaaggtcgt cgtgacgagc gacaacccgc gcagcgagga gccgcagaag atcatcgatc agatcgtcgc cggcatgcgg gacgccgcga acgcgcgccg agtcgaggac cgcgcgagcg cgatcctgca ggccgtgcgc ggcgccgcgc gcgaggacgt cgtgctgctc gccggcaagg ggcacgaggc gacgcaggag atcatgggca agaagcgtgc gttctccgat caggatcacg cgcgcctcgc gctcgccgcg cgcacgacgc agactcgcgg aggcggcgaa taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for ButhA.01643.a.A1.PW33427
PURIFICATION DATe: 9/2/2011
CONCENTRATION: 29.62mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 11
VIAL VOLUME: 200µl
PERCENT IDENTITY: 62
PERCENT COVERAGE: 96
Protocol Notes
notes unavailable
Validated AA Sequence
MSAARSSHPA HQQIAAALAW LRAHVASGAQ LHADTRSLQA GDVFVAYAVD GADNRPFIAD ALARGAAAVL YQPEGFPAAG LDPATSLAVP ALDALAGDIA SGWYGDPSDA MFAVGVTGTN GKTSCTQWIA AALTALREPC AVIGTLGSGM PGQLVHTGFT TPDAPQLQRS LAQLRDAGAQ AVAMEVSSHA LHQGRVNGTA FDVAVFTNLT QDHLDYHRTF DAYEAAKAKL FAWRGLHCAV VNRDDPAXPP PARESRRPRE DDRVRHRCAG ARRRRRSRAR RDERPRDRDG HRVSRDFAVG RSGRRGRHAR HVQREQSARG ARHAARGRRA VRRGARRNRA ARIGQRPDAA ARRPAAERRA ARRRRLRAHA GRAREDARSA APDRAGARRR ARLHVRLRRR PRRDEAPADG RDRRAARGQG RRDERQPAQR GAAEDHRSDR RRHAGRRERA PSRGPRERDP AGRARRRARG RRAARRQGAR GDAGDHGQEA CVLRSGSRAP RARRAHDADS RRRR
Validated NT Sequence
tattcgccgc ctccgcgagt ctgcgtcgtg cgcgcggcga gcgcgaggcg cgcgtgatcc tgatcggaga acgcacgctt cttgcccatg atctcctgcg tcgcctcgtg ccccttgccg gcgagcagca cgacgtcctc gcgcgcggcg ccgcgcacgg cctgcaggat cgcgctcgcg cggtcctcga ctcggcgcgc gttcgcggcg tcccgcatgc cggcgacgat ctgatcgatg atcttctgcg gctcctcgct gcgcgggttg tcgctcgtca cgacgacctt gtccgcgagc cgctcggcga tcgcgcccat cagcgggcgc ttcgtcgcgt cgcggtcgcc gccgcagccg aacatgcaga cgagctcgcc gccgcgcgcc tgcgcgatcg ggcgcagcgc ttcgagcgtc ttctcgagcg cgtccggcgt gtgcgcgtaa tcgacgacga cgagcggctc gtcgttctgc agccggccgc cgagccgctg catccggccg ttgaccgatt cgagccgctc gatttcggcg agcgccgcgt cgaacggcac gtcggccgcg agcagcgtgc cgagcaccgc gagcagattg ctcacgttga acgtgccgag cgtgccgacc tcgacgtccg cttcgcccca cggcgaagtc acgcgaaacg cggtgcccgt cgcggtcgcg cggacgttcg tcgcgacgag ctcgcgatcg gcgtcggcgc cttgcgccgg cgcatcgatg ccgtacgcga tcgtcctcac gcggccgacg agattcgcga gcaggcggcg gccnggccgg atcgtcgcga ttgacaaccg cgcaatgcag cccgcgccag gcgaagagct ttgccttcgc ggcctcgtag gcgtcgaacg tgcggtggta atcgaggtga tcctgcgtga ggttcgtgaa caccgcgacg tcgaacgcgg tgccgttcac gcgcccctga tgcagcgcat gcgacgagac ttccatcgcg accgcctgag cgcccgcgtc gcgcagttgc gcgaggctgc gctgcagttg cggcgcatcg ggcgtcgtga agcccgtgtg gacgagctgg ccgggcattc cgctgccgag cgtgccgatc accgcgcacg gctcgcgcaa cgctgtgagc gcggccgcga tccattgcgt gcacgacgtc ttgccgttcg tgcccgtcac gccgaccgcg aacatcgcgt cgctcggatc gccgtaccag ccgctcgcga tatcgcccgc gagcgcgtcg agcgcgggca cggcgagcga cgtcgccggg tcgaggccgg cggccgggaa accttcgggc tgatacagca cggcggccgc gccgcgcgcg agcgcgtcgg caatgaacgg acgattatcc gcgccgtcga ccgcataggc gacgaacacg tcgcccgcct gcaggctgcg cgtgtcggcg tgcaattgcg cgccggacgc gacgtgcgcg cgcagccacg caagcgcggc cgcgatctgc tggtgcgccg gatgggaact gcgcgcggcg ctcatcgaac caggaccctg ggtctgagct tccaggg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSAARSSHP AHQQIAAALA WLRAHVASGA QLHADTRSLQ AGDVFVAYAV DGADNRPFIA DALARGAAAV LYQPEGFPAA GLDPATSLAV PALDALAGDI ASGWYGDPSD AMFAVGVTGT NGKTSCTQWI AAALTALREP CAVIGTLGSG MPGQLVHTGF TTPDAPQLQR SLAQLRDAGA QAVAMEVSSH ALHQGRVNGT AFDVAVFTNL TQDHLDYHRT FDAYEAAKAK LFAWRGLHCA VVNRDDPAGR RLLANLVGRV RTIAYGIDAP AQGADADREL VATNVRATAT GTAFRVTSPW GEADVEVGTL GTFNVSNLLA VLGTLLAADV PFDAALAEIE RLESVNGRMQ RLGGRLQNDE PLVVVDYAHT PDALEKTLEA LRPIAQARGG ELVCMFGCGG DRDATKRPLM GAIAERLADK VVVTSDNPRS EEPQKIIDQI VAGMRDAANA RRVEDRASAI LQAVRGAARE DVVLLAGKGH EATQEIMGKK RAFSDQDHAR LALAARTTQT RGGGE
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCGCCGCG CGCAGTTCCC ATCCGGCGCA CCAGCAGATC GCGGCCGCGC TTGCGTGGCT GCGCGCGCAC GTCGCGTCCG GCGCGCAATT GCACGCCGAC ACGCGCAGCC TGCAGGCGGG CGACGTGTTC GTCGCCTATG CGGTCGACGG CGCGGATAAT CGTCCGTTCA TTGCCGACGC GCTCGCGCGC GGCGCGGCCG CCGTGCTGTA TCAGCCCGAA GGTTTCCCGG CCGCCGGCCT CGACCCGGCG ACGTCGCTCG CCGTGCCCGC GCTCGACGCG CTCGCGGGCG ATATCGCGAG CGGCTGGTAC GGCGATCCGA GCGACGCGAT GTTCGCGGTC GGCGTGACGG GCACGAACGG CAAGACGTCG TGCACGCAAT GGATCGCGGC CGCGCTCACA GCGTTGCGCG AGCCGTGCGC GGTGATCGGC ACGCTCGGCA GCGGAATGCC CGGCCAGCTC GTCCACACGG GCTTCACGAC GCCCGATGCG CCGCAACTGC AGCGCAGCCT CGCGCAACTG CGCGACGCGG GCGCTCAGGC GGTCGCGATG GAAGTCTCGT CGCATGCGCT GCATCAGGGG CGCGTGAACG GCACCGCGTT CGACGTCGCG GTGTTCACGA ACCTCACGCA GGATCACCTC GATTACCACC GCACGTTCGA CGCCTACGAG GCCGCGAAGG CAAAGCTCTT CGCCTGGCGC GGGCTGCATT GCGCGGTTGT CAATCGCGAC GATCCGGCCG GCCGCCGCCT GCTCGCGAAT CTCGTCGGCC GCGTGAGGAC GATCGCGTAC GGCATCGATG CGCCGGCGCA AGGCGCCGAC GCCGATCGCG AGCTCGTCGC GACGAACGTC CGCGCGACCG CGACGGGCAC CGCGTTTCGC GTGACTTCGC CGTGGGGCGA AGCGGACGTC GAGGTCGGCA CGCTCGGCAC GTTCAACGTG AGCAATCTGC TCGCGGTGCT CGGCACGCTG CTCGCGGCCG ACGTGCCGTT CGACGCGGCG CTCGCCGAAA TCGAGCGGCT CGAATCGGTC AACGGCCGGA TGCAGCGGCT CGGCGGCCGG CTGCAGAACG ACGAGCCGCT CGTCGTCGTC GATTACGCGC ACACGCCGGA CGCGCTCGAG AAGACGCTCG AAGCGCTGCG CCCGATCGCG CAGGCGCGCG GCGGCGAGCT CGTCTGCATG TTCGGCTGCG GCGGCGACCG CGACGCGACG AAGCGCCCGC TGATGGGCGC GATCGCCGAG CGGCTCGCGG ACAAGGTCGT CGTGACGAGC GACAACCCGC GCAGCGAGGA GCCGCAGAAG ATCATCGATC AGATCGTCGC CGGCATGCGG GACGCCGCGA ACGCGCGCCG AGTCGAGGAC CGCGCGAGCG CGATCCTGCA GGCCGTGCGC GGCGCCGCGC GCGAGGACGT CGTGCTGCTC GCCGGCAAGG GGCACGAGGC GACGCAGGAG ATCATGGGCA AGAAGCGTGC GTTCTCCGAT CAGGATCACG CGCGCCTCGC GCTCGCCGCG CGCACGACGC AGACTCGCGG AGGCGGCGAA TAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA