ButhA.01570.a

2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase (BTH_I1313)

CENTER ID: ButhA.01570.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.01570.a.A1.GE32978 full length 1 175
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.4080
RefSeq: YP_441860.1
UniProt: Q2SYY9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTVAYLGLGA NLGDARQALK DAVVCLAQQH AITVLGKSSL YRTAPIDAGG GDYYNCAVKI DTTLAARALL ALCQKIEHHF GRERPYRNAP RTLDIDILLY GEQAIDEPDL VVPHPRLTER AFALVPLVEI EPTLAIPARG RADEFLAGVA HQRIEKVQTC QCLAMRAAAE KNRCR
NT Sequence
atgacggtcg cttatctcgg gctgggggcc aacctcggcg atgcgcgcca ggccttgaag gacgcagtgg tctgcctcgc ccagcagcac gccatcacgg tgctcggcaa gtcgagcctg tatcgcacgg cgcccatcga cgcgggcggc ggcgactact acaactgcgc cgtgaagatc gacacgacgc tcgccgcgcg cgcgcttctc gcgctctgcc agaagatcga gcaccacttc ggccgcgagc ggccgtaccg caacgcgccg cgcacgctcg acatcgacat cctgctctac ggtgaacagg cgatcgacga acccgacctc gtcgtcccgc atccgcgcct gaccgagcgc gcgttcgcgc tcgtgccgct cgtcgagatc gagccgactc tcgccattcc cgcgcgcggc cgcgccgacg aattcctcgc cggcgtcgcg catcagcgca tcgagaaagt ccagacctgc cagtgcctgg cgatgcgggc cgccgccgaa aagaaccgct gccgatga
Details for ButhA.01570.a.A1.GE32978
HARVESTED ON: 8/16/2011
SEQUENCED ON: 8/22/2011
EXPECTED MW: 21kDa
OBSERVED MW: 24kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 89
Validated AA Sequence
MTVAYLGLGA NLGDARQALK DAVVCLAQQH AITVLGKSSL YRTAPIDAGG GDYYNCAVKI DTTLAARALL ALCQKIEHHF GRERPYRNAP RTLDIDILLY GEQAIDEPDL VVPHPRLTER AFALVPLVEI EPTLAIPARG RADEFLAGVA HQRIEKVQTC QCLAMRAAAE KNRCR
Validated NT Sequence
ttattatcgg cagcggttct tttcggcggc ggcccgcatc gccaggcact ggcaggtctg gactttctcg atgcgctgat gcgcgacgcc ggcgaggaat tcgtcggcgc ggccgcgcgc gggaatggcg agagtcggct cgatctcgac gagcggcacg agcgcgaacg cgcgctcggt caggcgcgga tgcgggacga cgaggtcggg ttcgtcgatc gcctgttcac cgtagagcag gatgtcgatg tcgagcgtgc gcggcgcgtt gcggtacggc cgctcgcggc cgaagtggtg ctcgatcttc tggcagagcg cgagaagcgc gcgcgcggcg agcgtcgtgt cgatcttcac ggcgcagttg tagtagtcgc cgccgcccgc gtcgatgggc gccgtgcgat acaggctcga cttgccgagc accgtgatgg cgtgctgctg ggcgaggcag accactgcgt ccttcaaggc ctggcgcgca tcgccgaggt tggcccccag cccgagataa gcgaccgtca tcgaaccagg accctgggnt ctgagcttcc agggtaccca tatgatggtg atggtgatga gccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTVAYLGLG ANLGDARQAL KDAVVCLAQQ HAITVLGKSS LYRTAPIDAG GGDYYNCAVK IDTTLAARAL LALCQKIEHH FGRERPYRNA PRTLDIDILL YGEQAIDEPD LVVPHPRLTE RAFALVPLVE IEPTLAIPAR GRADEFLAGV AHQRIEKVQT CQCLAMRAAA EKNRCR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacggtcgct tatctcgggc tgggggccaa cctcggcgat gcgcgccagg ccttgaagga cgcagtggtc tgcctcgccc agcagcacgc catcacggtg ctcggcaagt cgagcctgta tcgcacggcg cccatcgacg cgggcggcgg cgactactac aactgcgccg tgaagatcga cacgacgctc gccgcgcgcg cgcttctcgc gctctgccag aagatcgagc accacttcgg ccgcgagcgg ccgtaccgca acgcgccgcg cacgctcgac atcgacatcc tgctctacgg tgaacaggcg atcgacgaac ccgacctcgt cgtcccgcat ccgcgcctga ccgagcgcgc gttcgcgctc gtgccgctcg tcgagatcga gccgactctc gccattcccg cgcgcggccg cgccgacgaa ttcctcgccg gcgtcgcgca tcagcgcatc gagaaagtcc agacctgcca gtgcctggcg atgcgggccg ccgccgaaaa gaaccgctgc cgataaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa