ButhA.01463.a

Guanylate kinase (EC 2.7.4.8) (GMP kinase) (BTH_I1586) (gmk)

CENTER ID: ButhA.01463.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.01463.a.A1.GE32767 full length 1 227
ButhA.01463.a.AE1.GE44048 full length 1 227
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.01463.a.A1.PW33371 full length 1 227

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.4373
RefSeq: YP_442127.1
UniProt: Q2SY72

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTDSNRGGAA AHSLHAGVYP GNLFMVVAPS GAGKSTLVNA LLSKDPEICL SISYTTRKPR SGEQDGQHYH FTTVEDFRAR HASHEFLESA EVHGNYYGTS RVWIEEQMKS GHDVLLEIDW QGAQQVKKQF RNAVGIFILP PSLAALEERL KKRGQDEPNV ITRRLLAAGS EIAHAAEAEY VVINETFEHA LAELECIVAA TRLRFTSQYA RHAELFVELG IHLPHAE
NT Sequence
atgaccgatt cgaaccgcgg cggcgcggca gcccattcgc tgcacgccgg cgtctatccc ggcaatctct tcatggtcgt cgcgccgtcg ggcgcgggca agtcgacgct cgtcaacgcg ctgctgtcga aggatccgga gatctgtctg tcgatttcgt acacgacgcg caagccgcgt tccggcgagc aggacggcca gcactatcac ttcacgaccg tcgaggattt ccgcgcgcgc cacgcgagcc acgagtttct ggagagcgcg gaggtgcacg gcaactacta cggcacgtcg cgcgtgtgga tcgaagagca gatgaagagc ggccacgatg tgctgctcga aatcgattgg cagggcgcgc agcaagtgaa gaagcagttt cgcaacgcgg tcggcatctt cattctgccg ccgtcgctcg cggcgctcga ggagcggctg aaaaagcgcg ggcaggacga gccgaacgtg atcacgcggc gcctgctcgc cgcaggcagc gagatcgcgc acgcggccga agcggagtac gtcgtgatca acgagacctt cgagcacgcg cttgccgagc tcgaatgcat cgtcgccgcg acgcgcctgc gcttcacgtc gcaatatgcg cggcatgccg aactattcgt cgagctcggc attcatctgc cgcatgcgga gtga
Details for ButhA.01463.a.A1.GE32767
HARVESTED ON: 6/20/2011
SEQUENCED ON: 7/19/2011
EXPECTED MW: 27kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTDSNRGGA AAHSLHAGVY PGNLFMVVAP SGAGKSTLVN ALLSKDPEIC LSISYTTRKP RSGEQDGQHY HFTTVEDFRA RHASHEFLES AEVHGNYYGT SRVWIEEQMK SGHDVLLEID WQGAQQVKKQ FRNAVGIFIL PPSLAALEER LKKRGQDEPN VITRRLLAAG SEIAHAAEAE YVVINETFEH ALAELECIVA ATRLRFTSQY ARHAELFVEL GIHLPHAE
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaccg attcgaaccg cggcggcgcg gcagcccatt cgctgcacgc cggcgtctat cccggcaatc tcttcatggt cgtcgcgccg tcgggcgcgg gcaagtcgac gctcgtcaac gcgctgctgt cgaaggatcc ggagatctgt ctgtcgattt cgtacacgac gcgcaagccg cgttccggcg agcaggacgg ccagcactat cacttcacga ccgtcgagga tttccgcgcg cgccacgcga gccacgagtt tctggagagc gcggaggtgc acggcaacta ctacggcacg tcgcgcgtgt ggatcgaaga gcagatgaag agcggccacg atgtgctgct cgaaatcgat tggcagggcg cgcagcaagt gaagaagcag tttcgcaacg cggtcggcat cttcattctg ccgccgtcgc tcgcggcgct cgaggagcgg ctgaaaaagc gcgggcagga cgagccgaac gtgatcacgc ggcgcctgct cgccgcaggc agcgagatcg cgcacgcggc cgaagcggag tacgtcgtga tcaacgagac cttcgagcac gcgcttgccg agctcgaatg catcgtcgcc gcgacgcgcc tgcgcttcac gtcgcaatat gcgcggcatg ccgaactatt cgtcgagctc ggcattcatc tgccgcatgc ggagtaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTDSNRGGA AAHSLHAGVY PGNLFMVVAP SGAGKSTLVN ALLSKDPEIC LSISYTTRKP RSGEQDGQHY HFTTVEDFRA RHASHEFLES AEVHGNYYGT SRVWIEEQMK SGHDVLLEID WQGAQQVKKQ FRNAVGIFIL PPSLAALEER LKKRGQDEPN VITRRLLAAG SEIAHAAEAE YVVINETFEH ALAELECIVA ATRLRFTSQY ARHAELFVEL GIHLPHAE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccgattcg aaccgcggcg gcgcggcagc ccattcgctg cacgccggcg tctatcccgg caatctcttc atggtcgtcg cgccgtcggg cgcgggcaag tcgacgctcg tcaacgcgct gctgtcgaag gatccggaga tctgtctgtc gatttcgtac acgacgcgca agccgcgttc cggcgagcag gacggccagc actatcactt cacgaccgtc gaggatttcc gcgcgcgcca cgcgagccac gagtttctgg agagcgcgga ggtgcacggc aactactacg gcacgtcgcg cgtgtggatc gaagagcaga tgaagagcgg ccacgatgtg ctgctcgaaa tcgattggca gggcgcgcag caagtgaaga agcagtttcg caacgcggtc ggcatcttca ttctgccgcc gtcgctcgcg gcgctcgagg agcggctgaa aaagcgcggg caggacgagc cgaacgtgat cacgcggcgc ctgctcgccg caggcagcga gatcgcgcac gcggccgaag cggagtacgt cgtgatcaac gagaccttcg agcacgcgct tgccgagctc gaatgcatcg tcgccgcgac gcgcctgcgc ttcacgtcgc aatatgcgcg gcatgccgaa ctattcgtcg agctcggcat tcatctgccg catgcggagt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for ButhA.01463.a.AE1.GE44048
HARVESTED ON: 1/7/2021
SEQUENCED ON: 1/8/2021
EXPECTED MW: 26kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MTDSXRGGAA XHSLHAGVYP GNLFMVVAPS GAGKSTLVNA LLSKDPEICL SISYXTRKPR SGEQDGQHYH FTTVEDFRAR HASHEFLESA EVHGNYYGTS RVWIEEQMKS GHDVLLEIDW QGAQQVKKQF RNAVGIFILP PSLAALEERL KKRGQDEPNV ITRRLLAAGS EIAHAAEAEY VVINETFEHA LAELECIVAA TRLRFTSQYA RHAELFVELG IHLPHAEAGH HHHHH
Validated NT Sequence
tccgttaatg atgatgatgg tggtgcccag cctccgcatg cggcagatga atgccgagct cgacgaatag ttcggcatgc cgcgcatatt gcgacgtgaa gcgcaggcgc gtngcggcga cgatgcattc gagctcggca agcgcgtgct cgaaggtctc gttgatcacg acgtactccg cttcggccgc gtgcgcgatc tcgctgcctg cggcgagcag gcgccgcgtg atcacgttcg gctcgtcctg cccgcgcttt ttcagccgct cctcgagcgc cgcgagcgac ggcggcagaa tgaagatgcc gaccgcgttg cgaaactgct tcttcacttg ctgcgcgccc tgccaatcga tttcgagcag cacatcgtgg ccgctcttca tctgctcttc gatccacacg cgcgacgtgc cgtagtagtt gccgtgcacc tccgcgctct ccagaaactc gtggctcgcg tggcgcgcgc ggaaatcctc gacggtcgtg aagtgatagt gctggccgtc ctgctcgccg gaacgcggct tgcgcgtcgn gtacgaaatc gacagacaga tctccggatc cttcgacagc agcgcgttga cgagcgtcga cttgcccgcg cccgacggcg cgacgaccat gaagagattg ccgggataga cgccggcgtg cagcgaatgg gnngccgcgc cgccgcgntt cgaatcggtc atgggagaga
Expected Protein Sequence
MTDSNRGGAA AHSLHAGVYP GNLFMVVAPS GAGKSTLVNA LLSKDPEICL SISYTTRKPR SGEQDGQHYH FTTVEDFRAR HASHEFLESA EVHGNYYGTS RVWIEEQMKS GHDVLLEIDW QGAQQVKKQF RNAVGIFILP PSLAALEERL KKRGQDEPNV ITRRLLAAGS EIAHAAEAEY VVINETFEHA LAELECIVAA TRLRFTSQYA RHAELFVELG IHLPHAEGHH HHHH
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag tctctcccat gaccgattcg aaccgcggcg gcgcggcagc ccattcgctg cacgccggcg tctatcccgg caatctcttc atggtcgtcg cgccgtcggg cgcgggcaag tcgacgctcg tcaacgcgct gctgtcgaag gatccggaga tctgtctgtc gatttcgtac acgacgcgca agccgcgttc cggcgagcag gacggccagc actatcactt cacgaccgtc gaggatttcc gcgcgcgcca cgcgagccac gagtttctgg agagcgcgga ggtgcacggc aactactacg gcacgtcgcg cgtgtggatc gaagagcaga tgaagagcgg ccacgatgtg ctgctcgaaa tcgattggca gggcgcgcag caagtgaaga agcagtttcg caacgcggtc ggcatcttca ttctgccgcc gtcgctcgcg gcgctcgagg agcggctgaa aaagcgcggg caggacgagc cgaacgtgat cacgcggcgc ctgctcgccg caggcagcga gatcgcgcac gcggccgaag cggagtacgt cgtgatcaac gagaccttcg agcacgcgct tgccgagctc gaatgcatcg tcgccgcgac gcgcctgcgc ttcacgtcgc aatatgcgcg gcatgccgaa ctattcgtcg agctcggcat tcatctgccg catgcggagg ggcaccacca tcatcatcat taacggatcc gaattcgagc tccgtcgaca agcttgcggc cgcactcgag caccaccacc accaccactg agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggat
Details for ButhA.01463.a.A1.PW33371
PURIFICATION DATe: 10/21/2011
CONCENTRATION: 26.75mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 9
VIAL VOLUME: 50µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTDSNRGGA AAHSLHAGVY PGNLFMVVAP SGAGKSTLVN ALLSKDPEIC LSISYTTRKP RSGEQDGQHY HFTTVEDFRA RHASHEFLES AEVHGNYYGT SRVWIEEQMK SGHDVLLEID WQGAQQVKKQ FRNAVGIFIL PPSLAALEER LKKRGQDEPN VITRRLLAAG SEIAHAAEAE YVVINETFEH ALAELECIVA ATRLRFTSQY ARHAELFVEL GIHLPHAE
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaccg attcgaaccg cggcggcgcg gcagcccatt cgctgcacgc cggcgtctat cccggcaatc tcttcatggt cgtcgcgccg tcgggcgcgg gcaagtcgac gctcgtcaac gcgctgctgt cgaaggatcc ggagatctgt ctgtcgattt cgtacacgac gcgcaagccg cgttccggcg agcaggacgg ccagcactat cacttcacga ccgtcgagga tttccgcgcg cgccacgcga gccacgagtt tctggagagc gcggaggtgc acggcaacta ctacggcacg tcgcgcgtgt ggatcgaaga gcagatgaag agcggccacg atgtgctgct cgaaatcgat tggcagggcg cgcagcaagt gaagaagcag tttcgcaacg cggtcggcat cttcattctg ccgccgtcgc tcgcggcgct cgaggagcgg ctgaaaaagc gcgggcagga cgagccgaac gtgatcacgc ggcgcctgct cgccgcaggc agcgagatcg cgcacgcggc cgaagcggag tacgtcgtga tcaacgagac cttcgagcac gcgcttgccg agctcgaatg catcgtcgcc gcgacgcgcc tgcgcttcac gtcgcaatat gcgcggcatg ccgaactatt cgtcgagctc ggcattcatc tgccgcatgc ggagtaataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTDSNRGGA AAHSLHAGVY PGNLFMVVAP SGAGKSTLVN ALLSKDPEIC LSISYTTRKP RSGEQDGQHY HFTTVEDFRA RHASHEFLES AEVHGNYYGT SRVWIEEQMK SGHDVLLEID WQGAQQVKKQ FRNAVGIFIL PPSLAALEER LKKRGQDEPN VITRRLLAAG SEIAHAAEAE YVVINETFEH ALAELECIVA ATRLRFTSQY ARHAELFVEL GIHLPHAE
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACCGATTCG AACCGCGGCG GCGCGGCAGC CCATTCGCTG CACGCCGGCG TCTATCCCGG CAATCTCTTC ATGGTCGTCG CGCCGTCGGG CGCGGGCAAG TCGACGCTCG TCAACGCGCT GCTGTCGAAG GATCCGGAGA TCTGTCTGTC GATTTCGTAC ACGACGCGCA AGCCGCGTTC CGGCGAGCAG GACGGCCAGC ACTATCACTT CACGACCGTC GAGGATTTCC GCGCGCGCCA CGCGAGCCAC GAGTTTCTGG AGAGCGCGGA GGTGCACGGC AACTACTACG GCACGTCGCG CGTGTGGATC GAAGAGCAGA TGAAGAGCGG CCACGATGTG CTGCTCGAAA TCGATTGGCA GGGCGCGCAG CAAGTGAAGA AGCAGTTTCG CAACGCGGTC GGCATCTTCA TTCTGCCGCC GTCGCTCGCG GCGCTCGAGG AGCGGCTGAA AAAGCGCGGG CAGGACGAGC CGAACGTGAT CACGCGGCGC CTGCTCGCCG CAGGCAGCGA GATCGCGCAC GCGGCCGAAG CGGAGTACGT CGTGATCAAC GAGACCTTCG AGCACGCGCT TGCCGAGCTC GAATGCATCG TCGCCGCGAC GCGCCTGCGC TTCACGTCGC AATATGCGCG GCATGCCGAA CTATTCGTCG AGCTCGGCAT TCATCTGCCG CATGCGGAGT AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA