ButhA.01417.a
Gamma-glutamyl phosphate reductase (GPR) (EC 1.2.1.41) (Glutamate-5-semialdehyde dehydrogenase) (Glutamyl-gamma-semialdehyde dehydrogenase) (GSA dehydrogenase) (BTH_I1214) (proA)
| CENTER ID: | ButhA.01417.a |
| ORGANISM: | Burkholderia thailandensis E264 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.01417.a.A1.GE32981 | full length | 1 | 423 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.01417.a.A1.PS01223 | full length | 1 | 423 | ||
| ButhA.01417.a.A1.PS01224 | full length | 1 | 423 |
Structures
4GHK
DEPOSITED: 8/7/2012
DETERMINATION: XRay
CLONE: ButhA.01417.a.A1.GE32981
PROTEIN: ButhA.01417.a.A1.PS01224
Publications by SSGCID
Combining functional and structural genomics to sample the essential Burkholderia structome.
Abendroth J, Armour B, Barrett L, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Fox D, Gallagher LA, Gardberg AS, Gillespie A, Manoil C, Myler PJ, Nakazawa-Hewitt S, Napuli A, Nguyen MT, Patrapuvich R, Phan I, Stacy R, Staker BL, Stewart LJ, Van Voorhis WC
PLoS ONE - 2012
volume 8, issue 1, pages e53851
PMID: 23382856; PMCID: PMC3561365
PDB:
3D63,
3DAH,
3EIZ,
3EJ2,
3EK2,
3EZO,
3F0F,
3FTP,
3GK0,
3GK3,
3GVF,
3GWA,
3GWE,
3IML,
3SZ8,
3T4C,
3TML,
3TMQ,
3TXY,
3U7J,
3UE9,
3UK1,
3UK2,
3UND,
3UPT,
3URR,
3UW1,
3UW2,
3UW3,
3V2I,
3V7N,
3V8H,
3V9O,
3V9P,
3VAV,
4DDO,
4DFE,
4DHE,
4DHK,
4DUT,
4DZ4,
4E4T,
4E51,
4EFI,
4EG0,
4EGJ,
4EK2,
4EQY,
4EWG,
4EX5,
4EXQ,
4F2G,
4F32,
4F3N,
4F3Y,
4F4H,
4F7D,
4FK8,
4FRY,
4G1K,
4G67,
4G6Z,
4GHK,
4H3Y,
4H3Z,
4H4G
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_127763 |
| PATRIC ID: | fig|271848.6.peg.3970 |
| RefSeq: | YP_441761.1 |
| UniProt: | Q2SZ88 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDIDQYMTDV GRRARRASRS IARASTAAKN AALEAVARAI ERDAGALKAA NARDVARAKD KGLDAAFVDR LTLSDKALKT MVEGLRQVAT LPDPIGEMSN LKYRPSGIQV GQMRVPLGVI GIIYESRPNV TIDAAALCLK SGNATILRGG SEALESNTAL AKLIGEGLAE AGLPQDTVQV VETADRAAVG RLITMTEYVD VIVPRGGKSL IERLINEARV PMIKHLDGIC HVYVDDRASV TKALTVCDNA KTHRYGTCNT METLLVARGI APAVLSPLGR LYREKGVELR VDADARAVLE AAGVGPLVDA TDEDWRTEYL APVLAIKIVD GIDAAIEHIN EYGSHHTDAI VTEDHDRAMR FLREVDSASV MVNASTRFAD GFEFGLGAEI GISNDKLHAR GPVGLEGLTS LKYVVLGHGE GRQ
NT Sequence
atggatatcg atcagtacat gacggacgtc ggccgccgcg cgcggcgtgc ctcgcgctcg atcgcgcgcg cgtccaccgc ggcgaagaat gccgcgctcg aagcggtcgc gcgggcgatc gagcgcgacg ctggcgcgct gaaggcggcc aacgcgcgcg acgtcgcgcg cgcgaaggac aagggcctcg acgcggcgtt cgtcgatcgc ctgacgctgt cggacaaggc gctgaagacg atggtcgaag ggctgcgcca ggtcgcgacg ctgccggacc cgatcggtga gatgagcaac ctcaagtacc gcccgagcgg aatccaggtc ggccagatgc gcgtgccgct cggcgtgatc gggatcatct acgagtcgcg cccgaacgtg acgatcgatg cggccgcgct gtgcctgaag tccggcaatg cgacgatcct gcgcggcggc tcggaagcgc tcgaatcgaa caccgcgctc gcgaagctga tcggcgaagg gctcgccgag gcggggctgc cgcaggacac ggtgcaggtc gtcgagacgg ccgatcgcgc ggcggtgggc aggctgatca cgatgaccga atatgtcgac gtgatcgtgc cgcgcggcgg caagagcctg atcgagcggc tgatcaacga ggcgcgcgtg ccgatgatca agcacctcga cggcatttgc cacgtgtacg tggacgaccg cgcgagcgtg acgaaggcgc tcactgtctg cgacaacgcg aagacgcatc gctacggcac ctgcaacacg atggagacgc tgctcgtcgc gcgcgggatc gcgcccgcgg tgctgtcgcc gctcggccgg ctgtatcgcg agaagggcgt cgagctgcgc gtcgacgccg acgcgcgcgc ggtgctcgag gcggcgggcg tcggtccgct cgtcgacgcg accgatgaag actggcgcac cgaatatctc gcgccggtgc tcgcgatcaa gatcgtcgac ggcatcgacg ccgcgatcga gcacatcaac gagtacggct cgcatcacac cgatgcgatc gtcaccgagg atcacgaccg cgcgatgcgc ttcctgcgcg aagtcgattc ggcgagtgtg atggtgaacg cgtcgacgcg cttcgcggac ggcttcgaat tcggtctcgg cgcggagatc ggcatctcga acgacaagct gcatgcgcgc ggcccggtcg ggctcgaagg gctcacgtcg ctgaagtacg tggtgctcgg gcacggcgaa gggcgtcagt aa
Details for ButhA.01417.a.A1.GE32981
| HARVESTED ON: | 8/16/2011 |
| SEQUENCED ON: | 8/22/2011 |
| EXPECTED MW: | 47kDa |
| OBSERVED MW: | 47kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 95 |
Validated AA Sequence
MDIDQYMTDV GRRARRASRS IARASTAAKN AALEAVARAI ERDAGALKAA NARDVARAKD KGLDAAFVDR LTLSDKALKT MVEGLRQVAT LPDPIGEMSN LKYRPSGIQV GQMRVPLGVI GIIYESRPNV TIDAAALCLK SGNATILRGG SEALESNTAL AKLIGEGLAE AGLPQDTVQV VETADRAAVG RLITMTEYVD VIVPRGGKSL IERLINEARV PMIKHLDGIC HVYVDDRASV TKALTVCDNA KTHRYGTCNT METLLVARGI APAVLSPLGR LYREKGVELR VDADARAVLE AAGVGPLVDA TDEDWRTEYL APVLAIKIVD GIDAAIEHIN EYGSHHTDAI VTEDHDRAMR FLREVDSASV MVNASTRFAD GFEFGLGAEI GISNDKLHAR GPVGLEGLTS LKYVVLGHGE GRQ
Validated NT Sequence
ttattactga cgcccttcgc cgtgcccgag caccacgtac ttcagcgacg tgagcccttc gagcccgacc gggccgcgcg catgcagctt gtcgttcgag atgccgatct ccgcgccgag accgaattcg aagccgtccg cgaagcgcgt cgacgcgttc accatcacac tcgccgaatc gacttcgcgc aggaagcgca tcgcgcggtc gtgatcctcg gtgacgatcg catcggtgtg atgcgagccg tactcgttga tgtgctcgat cgcggcgtcg atgccgtcga cgatcttgat cgcgagcacc ggcgcgagat attcggtgcg ccagtcttca tcggtcgcgt cgacgagcgg accgacgccc gccgcctcga gcaccgcgcg cgcgtcggcg tcgacgcgca gctcgacgcc cttctcgcga tacagccggc cgagcggcga cagcaccgcg ggcgcgatcc cgcgcgcgac gagcagcgtc tccatcgtgt tgcaggtgcc gtagcgatgc gtcttcgcgt tgtcgcagac agtgagcgcc ttcgtcacgc tcgcgcggtc gtccacgtac acgtggcaaa tgccgtcgag gtgcttgatc atcggcacgc gcgcctcgtt gatcagccgc tcgatcaggc tcttgccgcc gcgcggcacg atcacgtcga catattcggt catcgtgatc agcctgccca ccgccgcgcg atcggccgtc tcgacgacct gcaccgtgtc ctgcggcagc cccgcctcgg cgagcccttc gccgatcagc ttcgcgagcg cggtgttcga ttcgagcgct tccgagccgc cgcgcaggat cgtcgcattg ccggacttca ggcacagcgc ggccgcatcg atcgtcacgt tcgggcgcga ctcgtagatg atcccgatca cgccgagcgg cacgcgcatc tggccgacct ggattccgct cgggcggtac ttgaggttgc tcatctcacc gatcgggtcc ggcagcgtcg cgacctggcg cagcccttcg accatcgtct tcagcgcctt gtccgacagc gtcaggcgat cgacgaacgc cgcgtcgagg cccttgtcct tcgcgcgcgc gacgtcgcgc gcgttggccg ccttcagcgc gccagcgtcg cgctcgatcg cccgcgcgac cgcttcgagc gcggcattct tcgccgcggt ggacgcgcgc gcgatcgagc gcgaggcacg ccgcgcgcgg cggccgacgt ccgtcatgta ctgatcgata tccatcgaac caggaccctg ggtctgagct tccagggt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDIDQYMTD VGRRARRASR SIARASTAAK NAALEAVARA IERDAGALKA ANARDVARAK DKGLDAAFVD RLTLSDKALK TMVEGLRQVA TLPDPIGEMS NLKYRPSGIQ VGQMRVPLGV IGIIYESRPN VTIDAAALCL KSGNATILRG GSEALESNTA LAKLIGEGLA EAGLPQDTVQ VVETADRAAV GRLITMTEYV DVIVPRGGKS LIERLINEAR VPMIKHLDGI CHVYVDDRAS VTKALTVCDN AKTHRYGTCN TMETLLVARG IAPAVLSPLG RLYREKGVEL RVDADARAVL EAAGVGPLVD ATDEDWRTEY LAPVLAIKIV DGIDAAIEHI NEYGSHHTDA IVTEDHDRAM RFLREVDSAS VMVNASTRFA DGFEFGLGAE IGISNDKLHA RGPVGLEGLT SLKYVVLGHG EGRQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggatatcgat cagtacatga cggacgtcgg ccgccgcgcg cggcgtgcct cgcgctcgat cgcgcgcgcg tccaccgcgg cgaagaatgc cgcgctcgaa gcggtcgcgc gggcgatcga gcgcgacgct ggcgcgctga aggcggccaa cgcgcgcgac gtcgcgcgcg cgaaggacaa gggcctcgac gcggcgttcg tcgatcgcct gacgctgtcg gacaaggcgc tgaagacgat ggtcgaaggg ctgcgccagg tcgcgacgct gccggacccg atcggtgaga tgagcaacct caagtaccgc ccgagcggaa tccaggtcgg ccagatgcgc gtgccgctcg gcgtgatcgg gatcatctac gagtcgcgcc cgaacgtgac gatcgatgcg gccgcgctgt gcctgaagtc cggcaatgcg acgatcctgc gcggcggctc ggaagcgctc gaatcgaaca ccgcgctcgc gaagctgatc ggcgaagggc tcgccgaggc ggggctgccg caggacacgg tgcaggtcgt cgagacggcc gatcgcgcgg cggtgggcag gctgatcacg atgaccgaat atgtcgacgt gatcgtgccg cgcggcggca agagcctgat cgagcggctg atcaacgagg cgcgcgtgcc gatgatcaag cacctcgacg gcatttgcca cgtgtacgtg gacgaccgcg cgagcgtgac gaaggcgctc actgtctgcg acaacgcgaa gacgcatcgc tacggcacct gcaacacgat ggagacgctg ctcgtcgcgc gcgggatcgc gcccgcggtg ctgtcgccgc tcggccggct gtatcgcgag aagggcgtcg agctgcgcgt cgacgccgac gcgcgcgcgg tgctcgaggc ggcgggcgtc ggtccgctcg tcgacgcgac cgatgaagac tggcgcaccg aatatctcgc gccggtgctc gcgatcaaga tcgtcgacgg catcgacgcc gcgatcgagc acatcaacga gtacggctcg catcacaccg atgcgatcgt caccgaggat cacgaccgcg cgatgcgctt cctgcgcgaa gtcgattcgg cgagtgtgat ggtgaacgcg tcgacgcgct tcgcggacgg cttcgaattc ggtctcggcg cggagatcgg catctcgaac gacaagctgc atgcgcgcgg cccggtcggg ctcgaagggc tcacgtcgct gaagtacgtg gtgctcgggc acggcgaagg gcgtcagtaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for ButhA.01417.a.A1.PS01223
| PURIFICATION DATe: | 10/5/2011 |
| CONCENTRATION: | 60.58mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 5 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 95 |
Protocol Notes
notes unavailable
Validated AA Sequence
MDIDQYMTDV GRRARRASRS IARASTAAKN AALEAVARAI ERDAGALKAA NARDVARAKD KGLDAAFVDR LTLSDKALKT MVEGLRQVAT LPDPIGEMSN LKYRPSGIQV GQMRVPLGVI GIIYESRPNV TIDAAALCLK SGNATILRGG SEALESNTAL AKLIGEGLAE AGLPQDTVQV VETADRAAVG RLITMTEYVD VIVPRGGKSL IERLINEARV PMIKHLDGIC HVYVDDRASV TKALTVCDNA KTHRYGTCNT METLLVARGI APAVLSPLGR LYREKGVELR VDADARAVLE AAGVGPLVDA TDEDWRTEYL APVLAIKIVD GIDAAIEHIN EYGSHHTDAI VTEDHDRAMR FLREVDSASV MVNASTRFAD GFEFGLGAEI GISNDKLHAR GPVGLEGLTS LKYVVLGHGE GRQ
Validated NT Sequence
ttattactga cgcccttcgc cgtgcccgag caccacgtac ttcagcgacg tgagcccttc gagcccgacc gggccgcgcg catgcagctt gtcgttcgag atgccgatct ccgcgccgag accgaattcg aagccgtccg cgaagcgcgt cgacgcgttc accatcacac tcgccgaatc gacttcgcgc aggaagcgca tcgcgcggtc gtgatcctcg gtgacgatcg catcggtgtg atgcgagccg tactcgttga tgtgctcgat cgcggcgtcg atgccgtcga cgatcttgat cgcgagcacc ggcgcgagat attcggtgcg ccagtcttca tcggtcgcgt cgacgagcgg accgacgccc gccgcctcga gcaccgcgcg cgcgtcggcg tcgacgcgca gctcgacgcc cttctcgcga tacagccggc cgagcggcga cagcaccgcg ggcgcgatcc cgcgcgcgac gagcagcgtc tccatcgtgt tgcaggtgcc gtagcgatgc gtcttcgcgt tgtcgcagac agtgagcgcc ttcgtcacgc tcgcgcggtc gtccacgtac acgtggcaaa tgccgtcgag gtgcttgatc atcggcacgc gcgcctcgtt gatcagccgc tcgatcaggc tcttgccgcc gcgcggcacg atcacgtcga catattcggt catcgtgatc agcctgccca ccgccgcgcg atcggccgtc tcgacgacct gcaccgtgtc ctgcggcagc cccgcctcgg cgagcccttc gccgatcagc ttcgcgagcg cggtgttcga ttcgagcgct tccgagccgc cgcgcaggat cgtcgcattg ccggacttca ggcacagcgc ggccgcatcg atcgtcacgt tcgggcgcga ctcgtagatg atcccgatca cgccgagcgg cacgcgcatc tggccgacct ggattccgct cgggcggtac ttgaggttgc tcatctcacc gatcgggtcc ggcagcgtcg cgacctggcg cagcccttcg accatcgtct tcagcgcctt gtccgacagc gtcaggcgat cgacgaacgc cgcgtcgagg cccttgtcct tcgcgcgcgc gacgtcgcgc gcgttggccg ccttcagcgc gccagcgtcg cgctcgatcg cccgcgcgac cgcttcgagc gcggcattct tcgccgcggt ggacgcgcgc gcgatcgagc gcgaggcacg ccgcgcgcgg cggccgacgt ccgtcatgta ctgatcgata tccatcgaac caggaccctg ggtctgagct tccagggt
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDIDQYMTD VGRRARRASR SIARASTAAK NAALEAVARA IERDAGALKA ANARDVARAK DKGLDAAFVD RLTLSDKALK TMVEGLRQVA TLPDPIGEMS NLKYRPSGIQ VGQMRVPLGV IGIIYESRPN VTIDAAALCL KSGNATILRG GSEALESNTA LAKLIGEGLA EAGLPQDTVQ VVETADRAAV GRLITMTEYV DVIVPRGGKS LIERLINEAR VPMIKHLDGI CHVYVDDRAS VTKALTVCDN AKTHRYGTCN TMETLLVARG IAPAVLSPLG RLYREKGVEL RVDADARAVL EAAGVGPLVD ATDEDWRTEY LAPVLAIKIV DGIDAAIEHI NEYGSHHTDA IVTEDHDRAM RFLREVDSAS VMVNASTRFA DGFEFGLGAE IGISNDKLHA RGPVGLEGLT SLKYVVLGHG EGRQ
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGATATCGAT CAGTACATGA CGGACGTCGG CCGCCGCGCG CGGCGTGCCT CGCGCTCGAT CGCGCGCGCG TCCACCGCGG CGAAGAATGC CGCGCTCGAA GCGGTCGCGC GGGCGATCGA GCGCGACGCT GGCGCGCTGA AGGCGGCCAA CGCGCGCGAC GTCGCGCGCG CGAAGGACAA GGGCCTCGAC GCGGCGTTCG TCGATCGCCT GACGCTGTCG GACAAGGCGC TGAAGACGAT GGTCGAAGGG CTGCGCCAGG TCGCGACGCT GCCGGACCCG ATCGGTGAGA TGAGCAACCT CAAGTACCGC CCGAGCGGAA TCCAGGTCGG CCAGATGCGC GTGCCGCTCG GCGTGATCGG GATCATCTAC GAGTCGCGCC CGAACGTGAC GATCGATGCG GCCGCGCTGT GCCTGAAGTC CGGCAATGCG ACGATCCTGC GCGGCGGCTC GGAAGCGCTC GAATCGAACA CCGCGCTCGC GAAGCTGATC GGCGAAGGGC TCGCCGAGGC GGGGCTGCCG CAGGACACGG TGCAGGTCGT CGAGACGGCC GATCGCGCGG CGGTGGGCAG GCTGATCACG ATGACCGAAT ATGTCGACGT GATCGTGCCG CGCGGCGGCA AGAGCCTGAT CGAGCGGCTG ATCAACGAGG CGCGCGTGCC GATGATCAAG CACCTCGACG GCATTTGCCA CGTGTACGTG GACGACCGCG CGAGCGTGAC GAAGGCGCTC ACTGTCTGCG ACAACGCGAA GACGCATCGC TACGGCACCT GCAACACGAT GGAGACGCTG CTCGTCGCGC GCGGGATCGC GCCCGCGGTG CTGTCGCCGC TCGGCCGGCT GTATCGCGAG AAGGGCGTCG AGCTGCGCGT CGACGCCGAC GCGCGCGCGG TGCTCGAGGC GGCGGGCGTC GGTCCGCTCG TCGACGCGAC CGATGAAGAC TGGCGCACCG AATATCTCGC GCCGGTGCTC GCGATCAAGA TCGTCGACGG CATCGACGCC GCGATCGAGC ACATCAACGA GTACGGCTCG CATCACACCG ATGCGATCGT CACCGAGGAT CACGACCGCG CGATGCGCTT CCTGCGCGAA GTCGATTCGG CGAGTGTGAT GGTGAACGCG TCGACGCGCT TCGCGGACGG CTTCGAATTC GGTCTCGGCG CGGAGATCGG CATCTCGAAC GACAAGCTGC ATGCGCGCGG CCCGGTCGGG CTCGAAGGGC TCACGTCGCT GAAGTACGTG GTGCTCGGGC ACGGCGAAGG GCGTCAGTAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA
Details for ButhA.01417.a.A1.PS01224
| PURIFICATION DATe: | 10/5/2011 |
| CONCENTRATION: | 60.58mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 1 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 95 |
Protocol Notes
notes unavailable
Validated AA Sequence
MDIDQYMTDV GRRARRASRS IARASTAAKN AALEAVARAI ERDAGALKAA NARDVARAKD KGLDAAFVDR LTLSDKALKT MVEGLRQVAT LPDPIGEMSN LKYRPSGIQV GQMRVPLGVI GIIYESRPNV TIDAAALCLK SGNATILRGG SEALESNTAL AKLIGEGLAE AGLPQDTVQV VETADRAAVG RLITMTEYVD VIVPRGGKSL IERLINEARV PMIKHLDGIC HVYVDDRASV TKALTVCDNA KTHRYGTCNT METLLVARGI APAVLSPLGR LYREKGVELR VDADARAVLE AAGVGPLVDA TDEDWRTEYL APVLAIKIVD GIDAAIEHIN EYGSHHTDAI VTEDHDRAMR FLREVDSASV MVNASTRFAD GFEFGLGAEI GISNDKLHAR GPVGLEGLTS LKYVVLGHGE GRQ
Validated NT Sequence
ttattactga cgcccttcgc cgtgcccgag caccacgtac ttcagcgacg tgagcccttc gagcccgacc gggccgcgcg catgcagctt gtcgttcgag atgccgatct ccgcgccgag accgaattcg aagccgtccg cgaagcgcgt cgacgcgttc accatcacac tcgccgaatc gacttcgcgc aggaagcgca tcgcgcggtc gtgatcctcg gtgacgatcg catcggtgtg atgcgagccg tactcgttga tgtgctcgat cgcggcgtcg atgccgtcga cgatcttgat cgcgagcacc ggcgcgagat attcggtgcg ccagtcttca tcggtcgcgt cgacgagcgg accgacgccc gccgcctcga gcaccgcgcg cgcgtcggcg tcgacgcgca gctcgacgcc cttctcgcga tacagccggc cgagcggcga cagcaccgcg ggcgcgatcc cgcgcgcgac gagcagcgtc tccatcgtgt tgcaggtgcc gtagcgatgc gtcttcgcgt tgtcgcagac agtgagcgcc ttcgtcacgc tcgcgcggtc gtccacgtac acgtggcaaa tgccgtcgag gtgcttgatc atcggcacgc gcgcctcgtt gatcagccgc tcgatcaggc tcttgccgcc gcgcggcacg atcacgtcga catattcggt catcgtgatc agcctgccca ccgccgcgcg atcggccgtc tcgacgacct gcaccgtgtc ctgcggcagc cccgcctcgg cgagcccttc gccgatcagc ttcgcgagcg cggtgttcga ttcgagcgct tccgagccgc cgcgcaggat cgtcgcattg ccggacttca ggcacagcgc ggccgcatcg atcgtcacgt tcgggcgcga ctcgtagatg atcccgatca cgccgagcgg cacgcgcatc tggccgacct ggattccgct cgggcggtac ttgaggttgc tcatctcacc gatcgggtcc ggcagcgtcg cgacctggcg cagcccttcg accatcgtct tcagcgcctt gtccgacagc gtcaggcgat cgacgaacgc cgcgtcgagg cccttgtcct tcgcgcgcgc gacgtcgcgc gcgttggccg ccttcagcgc gccagcgtcg cgctcgatcg cccgcgcgac cgcttcgagc gcggcattct tcgccgcggt ggacgcgcgc gcgatcgagc gcgaggcacg ccgcgcgcgg cggccgacgt ccgtcatgta ctgatcgata tccatcgaac caggaccctg ggtctgagct tccagggt
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDIDQYMTD VGRRARRASR SIARASTAAK NAALEAVARA IERDAGALKA ANARDVARAK DKGLDAAFVD RLTLSDKALK TMVEGLRQVA TLPDPIGEMS NLKYRPSGIQ VGQMRVPLGV IGIIYESRPN VTIDAAALCL KSGNATILRG GSEALESNTA LAKLIGEGLA EAGLPQDTVQ VVETADRAAV GRLITMTEYV DVIVPRGGKS LIERLINEAR VPMIKHLDGI CHVYVDDRAS VTKALTVCDN AKTHRYGTCN TMETLLVARG IAPAVLSPLG RLYREKGVEL RVDADARAVL EAAGVGPLVD ATDEDWRTEY LAPVLAIKIV DGIDAAIEHI NEYGSHHTDA IVTEDHDRAM RFLREVDSAS VMVNASTRFA DGFEFGLGAE IGISNDKLHA RGPVGLEGLT SLKYVVLGHG EGRQ
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGATATCGAT CAGTACATGA CGGACGTCGG CCGCCGCGCG CGGCGTGCCT CGCGCTCGAT CGCGCGCGCG TCCACCGCGG CGAAGAATGC CGCGCTCGAA GCGGTCGCGC GGGCGATCGA GCGCGACGCT GGCGCGCTGA AGGCGGCCAA CGCGCGCGAC GTCGCGCGCG CGAAGGACAA GGGCCTCGAC GCGGCGTTCG TCGATCGCCT GACGCTGTCG GACAAGGCGC TGAAGACGAT GGTCGAAGGG CTGCGCCAGG TCGCGACGCT GCCGGACCCG ATCGGTGAGA TGAGCAACCT CAAGTACCGC CCGAGCGGAA TCCAGGTCGG CCAGATGCGC GTGCCGCTCG GCGTGATCGG GATCATCTAC GAGTCGCGCC CGAACGTGAC GATCGATGCG GCCGCGCTGT GCCTGAAGTC CGGCAATGCG ACGATCCTGC GCGGCGGCTC GGAAGCGCTC GAATCGAACA CCGCGCTCGC GAAGCTGATC GGCGAAGGGC TCGCCGAGGC GGGGCTGCCG CAGGACACGG TGCAGGTCGT CGAGACGGCC GATCGCGCGG CGGTGGGCAG GCTGATCACG ATGACCGAAT ATGTCGACGT GATCGTGCCG CGCGGCGGCA AGAGCCTGAT CGAGCGGCTG ATCAACGAGG CGCGCGTGCC GATGATCAAG CACCTCGACG GCATTTGCCA CGTGTACGTG GACGACCGCG CGAGCGTGAC GAAGGCGCTC ACTGTCTGCG ACAACGCGAA GACGCATCGC TACGGCACCT GCAACACGAT GGAGACGCTG CTCGTCGCGC GCGGGATCGC GCCCGCGGTG CTGTCGCCGC TCGGCCGGCT GTATCGCGAG AAGGGCGTCG AGCTGCGCGT CGACGCCGAC GCGCGCGCGG TGCTCGAGGC GGCGGGCGTC GGTCCGCTCG TCGACGCGAC CGATGAAGAC TGGCGCACCG AATATCTCGC GCCGGTGCTC GCGATCAAGA TCGTCGACGG CATCGACGCC GCGATCGAGC ACATCAACGA GTACGGCTCG CATCACACCG ATGCGATCGT CACCGAGGAT CACGACCGCG CGATGCGCTT CCTGCGCGAA GTCGATTCGG CGAGTGTGAT GGTGAACGCG TCGACGCGCT TCGCGGACGG CTTCGAATTC GGTCTCGGCG CGGAGATCGG CATCTCGAAC GACAAGCTGC ATGCGCGCGG CCCGGTCGGG CTCGAAGGGC TCACGTCGCT GAAGTACGTG GTGCTCGGGC ACGGCGAAGG GCGTCAGTAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA