SSGCID: Target Page

ButhA.01416.a

Fructose-bisphosphate aldolase, class II, Calvin cycle subtype (EC 4.1.2.13) (BTH_I0665) (fba)

CENTER ID:	ButhA.01416.a
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.01416.a.A1.GE32766	full length		1	354

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.3364
RefSeq:	YP_441221.1
UniProt:	Q2T0S8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPLVSMRQLL DHAAENGYGL PAFNVNNLEQ VQAIMAAADE VGAPVIMQAS AGARKYAGEA FLRHLIEAAV ESYPHIPVVM HQDHGQSPAV CMAAIRSGFT SVMMDGSLEA DGKTVASYEY NVDVSRKVVE MAHSIGVTVE AELGVLGSLE TMKGDKEDGH GAEGTMTREQ LLTDPEQAAD FVKLTQCDAL AIAIGTSHGA YKFTKKPTGD ILSIQRIKEI HARIPNTHLV MHGSSSVPQD LLAEIREFGG DMKETYGVPV EEIQEGIKHG VRKVNIDTDL RLAITGAIRR YMFENPGKFD PRDYLKPARE AAKKICVARY LAFGCEGQAA KIKPVPLEKI AEKYKAGDLA QVVR

NT Sequence

atgcctctcg tatcaatgcg tcaactgctg gatcacgccg cggagaacgg ctacggcctg cccgcgttca acgtgaacaa cctggagcag gtgcaggcga tcatggcggc cgcggacgaa gtcggcgcgc ccgtgatcat gcaggcgtcg gcgggcgccc ggaagtacgc gggcgaggcg ttcctgcgcc acctgatcga agcggcggtc gagtcgtacc cgcacattcc cgtcgtgatg caccaggatc acggccagtc gcccgccgtc tgcatggcgg cgattcgcag cggcttcacg agcgtgatga tggacggctc gctcgaggcc gacggcaaga cggtcgcgtc gtacgaatac aacgtcgacg tgtcgaggaa ggtcgtcgag atggcgcact cgatcggcgt gacggtcgag gccgagctcg gcgtgctcgg ctcgctcgag acgatgaagg gcgacaagga ggacggccac ggcgcagaag gcacgatgac gcgcgagcaa ctgctgaccg atcccgagca ggcggccgat ttcgtcaagc tcacgcagtg cgacgcgctc gcgatcgcga tcggcacgtc gcacggcgcg tacaagttca cgaagaagcc gacgggcgac atcctgtcga tccagcggat caaggaaatc cacgcgcgca tcccgaacac gcacctcgtg atgcacggct cgtcgtcggt gccgcaggac ctgctcgcgg aaatccgcga attcggcggc gacatgaagg aaacctacgg cgtgcccgtc gaggaaatcc aggaaggcat caagcacggc gtgcgcaagg tcaacatcga caccgacctg cgtctcgcga tcacgggcgc gatccgccgc tacatgttcg agaatcctgg caagttcgat ccgcgcgatt acctgaagcc cgcgcgcgaa gcggcgaaga agatctgcgt cgcgcgctat ctggcgttcg gctgcgaagg ccaggcggcg aagatcaagc cggtgccgct cgagaagatc gccgagaagt acaaggcagg cgacctcgcg caagtggtgc gttga

Details for ButhA.01416.a.A1.GE32766

HARVESTED ON:	6/20/2011
SEQUENCED ON:	7/14/2011
EXPECTED MW:	40kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMPLVSMRQL LDHAAENGYG LPAFNVNNLE QVQAIMAAAD EVGAPVIMQA SAGARKYAGE AFLRHLIEAA VESYPHIPVV MHQDHGQSPA VCMAAIRSGF TSVMMDGSLE ADGKTVASYE YNVDVSRKVV EMAHSIGVTV EAELGVLGSL ETMKGDKEDG HGAEGTMTRE QLLTDPEQAA DFVKLTQCDA LAIAIGTSHG AYKFTKKPTG DILSIQRIKE IHARIPNTHL VMHGSSSVPQ DLLAEIREFG GDMKETYGVP VEEIQEGIKH GVRKVNIDTD LRLAITGAIR RYMFENPGKF DPRDYLKPAR EAAKKICVAR YLAFGCEGQA AKIKPVPLEK IAEKYKAGDL AQVVR

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcctc tcgtatcaat gcgtcaactg ctggatcacg ccgcggagaa cggctacggc ctgcccgcgt tcaacgtgaa caacctggag caggtgcagg cgatcatggc ggccgcggac gaagtcggcg cgcccgtgat catgcaggcg tcggcgggcg cccggaagta cgcgggcgag gcgttcctgc gccacctgat cgaagcggcg gtcgagtcgt acccgcacat tcccgtcgtg atgcaccagg atcacggcca gtcgcccgcc gtctgcatgg cggcgattcg cagcggcttc acgagcgtga tgatggacgg ctcgctcgag gccgacggca agacggtcgc gtcgtacgaa tacaacgtcg acgtgtcgag gaaggtcgtc gagatggcgc actcgatcgg cgtgacggtc gaggccgagc tcggcgtgct cggctcgctc gagacgatga agggcgacaa ggaggacggc cacggcgcag aaggcacgat gacgcgcgag caactgctga ccgatcccga gcaggcggcc gatttcgtca agctcacgca gtgcgacgcg ctcgcgatcg cgatcggcac gtcgcacggc gcgtacaagt tcacgaagaa gccgacgggc gacatcctgt cgatccagcg gatcaaggaa atccacgcgc gcatcccgaa cacgcacctc gtgatgcacg gctcgtcgtc ggtgccgcag gacctgctcg cggaaatccg cgaattcggc ggcgacatga aggaaaccta cggcgtgccc gtcgaggaaa tccaggaagg catcaagcac ggcgtgcgca aggtcaacat cgacaccgac ctgcgtctcg cgatcacggg cgcgatccgc cgctacatgt tcgagaatcc tggcaagttc gatccgcgcg attacctgaa gcccgcgcgc gaagcggcga agaagatctg cgtcgcgcgc tatctggcgt tcggctgcga aggccaggcg gcgaagatca agccggtgcc gctcgagaag atcgccgaga agtacaaggc aggcgacctc gcgcaagtgg tgcgttaata a

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcctctcgta tcaatgcgtc aactgctgga tcacgccgcg gagaacggct acggcctgcc cgcgttcaac gtgaacaacc tggagcaggt gcaggcgatc atggcggccg cggacgaagt cggcgcgccc gtgatcatgc aggcgtcggc gggcgcccgg aagtacgcgg gcgaggcgtt cctgcgccac ctgatcgaag cggcggtcga gtcgtacccg cacattcccg tcgtgatgca ccaggatcac ggccagtcgc ccgccgtctg catggcggcg attcgcagcg gcttcacgag cgtgatgatg gacggctcgc tcgaggccga cggcaagacg gtcgcgtcgt acgaatacaa cgtcgacgtg tcgaggaagg tcgtcgagat ggcgcactcg atcggcgtga cggtcgaggc cgagctcggc gtgctcggct cgctcgagac gatgaagggc gacaaggagg acggccacgg cgcagaaggc acgatgacgc gcgagcaact gctgaccgat cccgagcagg cggccgattt cgtcaagctc acgcagtgcg acgcgctcgc gatcgcgatc ggcacgtcgc acggcgcgta caagttcacg aagaagccga cgggcgacat cctgtcgatc cagcggatca aggaaatcca cgcgcgcatc ccgaacacgc acctcgtgat gcacggctcg tcgtcggtgc cgcaggacct gctcgcggaa atccgcgaat tcggcggcga catgaaggaa acctacggcg tgcccgtcga ggaaatccag gaaggcatca agcacggcgt gcgcaaggtc aacatcgaca ccgacctgcg tctcgcgatc acgggcgcga tccgccgcta catgttcgag aatcctggca agttcgatcc gcgcgattac ctgaagcccg cgcgcgaagc ggcgaagaag atctgcgtcg cgcgctatct ggcgttcggc tgcgaaggcc aggcggcgaa gatcaagccg gtgccgctcg agaagatcgc cgagaagtac aaggcaggcg acctcgcgca agtggtgcgt taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa