ButhA.01370.b

Phosphoglucosamine mutase (EC 5.4.2.10) (BTH_I2774) (glmM)

CENTER ID: ButhA.01370.b
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.01370.b.A1.GE32765 full length 1 452
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.01370.b.A1.PS01194 full length 1 452

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.5637
RefSeq: YP_443286.1
UniProt: Q2SUW3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGRRYFGTDG IRGKVGDAPI TPDFVLRLGY AAGKVLASAP GRAASGARPT VLIGKDTRVS GYMLEAALEA GFSAAGVDVM LAGPMPTPGV AYLTRALRLS AGVVISASHN PYHDNGIKFF SADGNKLPDE TEAEIEAWLD KSLDCAPSDG LGKARRLDDA AGRYIEFCKS TFPAAFDLRG MKLVVDCAHG AAYQVAPHVF HELGADVIPI GVAPNGFNIN DGVGATAPDA LMRAVRANHA DLGIALDGDA DRLLVVDHTG RLYNGDELLY VLVKDRIATN GQVEGAVGTL MTNLAVEVAL KDAGVQFVRA AVGDRYVLEQ LRERGWQLGA EGSGHILSLD RHSTGDGIVS ALLVLAALKR SGKTLAQMLE GVTLFPQKLI NVRMKPGADW KGSDAIRRAI GSAEQALAGS GRVLIRASGT EPVLRVMVEA RHAADANHHA EAIADAVKQA TA
NT Sequence
atgggacgtc gttatttcgg cacggacggc attcgcggca aggtcggcga cgcgccgatc acccccgatt tcgtgttgcg gctcggttac gcggcgggca aggtgctcgc gagcgcgcct ggccgcgcgg cgtcgggcgc gcggccgacg gtgctgatcg gcaaggacac gcgcgtgtcg ggctacatgc tcgaagccgc acttgaagca ggtttttccg cggcgggcgt cgacgtgatg ctcgcgggcc cgatgccgac gccgggcgtc gcgtatctga cgcgcgcgct gcggctgtcg gcgggcgtcg tgatcagcgc gtcgcacaat ccgtatcacg acaacggcat caagttcttc tcggccgacg gcaacaaact gccggacgaa accgaagccg agatcgaggc gtggctcgac aagtcgctcg attgcgcgcc gtcggatggg ctcggtaagg cgcggcgtct cgacgacgcg gccggccgct acatcgagtt ctgcaagagc acgttcccgg ctgcgttcga cctgcgcggg atgaagcttg tcgtcgattg cgcgcacggc gccgcgtatc aggtcgcgcc gcatgtattt cacgaacttg gcgcggatgt gatcccgatc ggcgtggcgc cgaacggctt caacatcaac gacggcgtcg gcgcgacggc gcccgacgcg ctgatgcgcg cggtgcgcgc gaaccatgcc gatctgggca ttgccctcga cggcgatgcg gatcgcctgc tcgtcgtcga ccatactggg cggctgtata acggcgatga gttgctgtac gtgctcgtca aggatcgcat tgcaactaac ggccaggttg agggggcggt gggcacgctg atgacgaatc tcgccgtcga agtcgcgctg aaagacgcgg gcgtgcagtt cgtgcgggcg gcggttggcg atcgctacgt gctcgagcag ttgcgcgagc gcggctggca actcggcgcc gagggctcgg gccacattct gtcgctcgat cggcattcga cgggcgacgg gatcgtctcg gcgctgctcg tgctcgccgc gctcaagcgc agcggcaaga cgctcgcgca gatgctcgaa ggcgtcacgc tgttcccgca gaagctgatc aacgtgcgga tgaagcctgg cgccgactgg aagggcagtg atgcgatccg ccgcgcgatc ggttcggccg agcaggcgct tgccggcagc ggccgcgtgc tgatccgcgc gtcggggacg gaacccgtgt tgcgggtgat ggtcgaggcg cggcacgcgg cggatgcgaa ccatcatgcg gaggcgattg ccgacgcggt caagcaggcg acggcgtaa
Details for ButhA.01370.b.A1.GE32765
HARVESTED ON: 6/20/2011
SEQUENCED ON: 7/14/2011
EXPECTED MW: 49kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGRRYFGTD GIRGKVGDAP ITPDFVLRLG YAAGKVLASA PGRAASGARP TVLIGKDTRV SGYMLEAALE AGFSAAGVDV MLAGPMPTPG VAYLTRALRL SAGVVISASH NPYHDNGIKF FSADGNKLPD ETEAEIEAWL DKSLDCAPSD GLGKARRLDD AAGRYIEFCK STFPAAFDLR GMKLVVDCAH GAAYQVAPHV FHELGADVIP IGVAPNGFNI NDGVGATAPD ALMRAVRANH ADLGIALDGD ADRLLVVDHT GRLYNGDELL YVLVKDRIAT NGQVEGAVGT LMTNLAVEVA LKDAGVQFVR AAVGDRYVLE QLRERGWQLG AEGSGHILSL DRHSTGDGIV SALLVLAALK RSGKTLAQML EGVTLFPQKL INVRMKPGAD WKGSDAIRRA IGSAEQALAG SGRVLIRASG TEPVLRVMVE ARHAADANHH AEAIADAVKQ AT
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgggac gtcgttattt cggcacggac ggcattcgcg gcaaggtcgg cgacgcgccg atcacccccg atttcgtgtt gcggctcggt tacgcggcgg gcaaggtgct cgcgagcgcg cctggccgcg cggcgtcggg cgcgcggccg acggtgctga tcggcaagga cacgcgcgtg tcgggctaca tgctcgaagc cgcacttgaa gcaggttttt ccgcggcggg cgtcgacgtg atgctcgcgg gcccgatgcc gacgccgggc gtcgcgtatc tgacgcgcgc gctgcggctg tcggcgggcg tcgtgatcag cgcgtcgcac aatccgtatc acgacaacgg catcaagttc ttctcggccg acggcaacaa actgccggac gaaaccgaag ccgagatcga ggcgtggctc gacaagtcgc tcgattgcgc gccgtcggat gggctcggta aggcgcggcg tctcgacgac gcggccggcc gctacatcga gttctgcaag agcacgttcc cggctgcgtt cgacctgcgc gggatgaagc ttgtcgtcga ttgcgcgcac ggcgccgcgt atcaggtcgc gccgcatgta tttcacgaac ttggcgcgga tgtgatcccg atcggcgtgg cgccgaacgg cttcaacatc aacgacggcg tcggcgcgac ggcgcccgac gcgctgatgc gcgcggtgcg cgcgaaccat gccgatctgg gcattgccct cgacggcgat gcggatcgcc tgctcgtcgt cgaccatact gggcggctgt ataacggcga tgagttgctg tacgtgctcg tcaaggatcg cattgcaact aacggccagg ttgagggggc ggtgggcacg ctgatgacga atctcgccgt cgaagtcgcg ctgaaagacg cgggcgtgca gttcgtgcgg gcggcggttg gcgatcgcta cgtgctcgag cagttgcgcg agcgcggctg gcaactcggc gccgagggct cgggccacat tctgtcgctc gatcggcatt cgacgggcga cgggatcgtc tcggcgctgc tcgtgctcgc cgcgctcaag cgcagcggca agacgctcgc gcagatgctc gaaggcgtca cgctgttccc gcagaagctg atcaacgtgc ggatgaagcc tggcgccgac tggaagggca gtgatgcgat ccgccgcgcg atcggttcgg ccgagcaggc gcttgccggc agcggccgcg tgctgatccg cgcgtcgggg acggaacccg tgttgcgggt gatggtcgag gcgcggcacg cggcggatgc gaaccatcat gcggaggcga ttgccgacgc ggtcaagcag gcgacggcg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGRRYFGTD GIRGKVGDAP ITPDFVLRLG YAAGKVLASA PGRAASGARP TVLIGKDTRV SGYMLEAALE AGFSAAGVDV MLAGPMPTPG VAYLTRALRL SAGVVISASH NPYHDNGIKF FSADGNKLPD ETEAEIEAWL DKSLDCAPSD GLGKARRLDD AAGRYIEFCK STFPAAFDLR GMKLVVDCAH GAAYQVAPHV FHELGADVIP IGVAPNGFNI NDGVGATAPD ALMRAVRANH ADLGIALDGD ADRLLVVDHT GRLYNGDELL YVLVKDRIAT NGQVEGAVGT LMTNLAVEVA LKDAGVQFVR AAVGDRYVLE QLRERGWQLG AEGSGHILSL DRHSTGDGIV SALLVLAALK RSGKTLAQML EGVTLFPQKL INVRMKPGAD WKGSDAIRRA IGSAEQALAG SGRVLIRASG TEPVLRVMVE ARHAADANHH AEAIADAVKQ ATA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggacgtcgt tatttcggca cggacggcat tcgcggcaag gtcggcgacg cgccgatcac ccccgatttc gtgttgcggc tcggttacgc ggcgggcaag gtgctcgcga gcgcgcctgg ccgcgcggcg tcgggcgcgc ggccgacggt gctgatcggc aaggacacgc gcgtgtcggg ctacatgctc gaagccgcac ttgaagcagg tttttccgcg gcgggcgtcg acgtgatgct cgcgggcccg atgccgacgc cgggcgtcgc gtatctgacg cgcgcgctgc ggctgtcggc gggcgtcgtg atcagcgcgt cgcacaatcc gtatcacgac aacggcatca agttcttctc ggccgacggc aacaaactgc cggacgaaac cgaagccgag atcgaggcgt ggctcgacaa gtcgctcgat tgcgcgccgt cggatgggct cggtaaggcg cggcgtctcg acgacgcggc cggccgctac atcgagttct gcaagagcac gttcccggct gcgttcgacc tgcgcgggat gaagcttgtc gtcgattgcg cgcacggcgc cgcgtatcag gtcgcgccgc atgtatttca cgaacttggc gcggatgtga tcccgatcgg cgtggcgccg aacggcttca acatcaacga cggcgtcggc gcgacggcgc ccgacgcgct gatgcgcgcg gtgcgcgcga accatgccga tctgggcatt gccctcgacg gcgatgcgga tcgcctgctc gtcgtcgacc atactgggcg gctgtataac ggcgatgagt tgctgtacgt gctcgtcaag gatcgcattg caactaacgg ccaggttgag ggggcggtgg gcacgctgat gacgaatctc gccgtcgaag tcgcgctgaa agacgcgggc gtgcagttcg tgcgggcggc ggttggcgat cgctacgtgc tcgagcagtt gcgcgagcgc ggctggcaac tcggcgccga gggctcgggc cacattctgt cgctcgatcg gcattcgacg ggcgacggga tcgtctcggc gctgctcgtg ctcgccgcgc tcaagcgcag cggcaagacg ctcgcgcaga tgctcgaagg cgtcacgctg ttcccgcaga agctgatcaa cgtgcggatg aagcctggcg ccgactggaa gggcagtgat gcgatccgcc gcgcgatcgg ttcggccgag caggcgcttg ccggcagcgg ccgcgtgctg atccgcgcgt cggggacgga acccgtgttg cgggtgatgg tcgaggcgcg gcacgcggcg gatgcgaacc atcatgcgga ggcgattgcc gacgcggtca agcaggcgac ggcgtaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for ButhA.01370.b.A1.PS01194
PURIFICATION DATe: 8/16/2011
CONCENTRATION: 41.79mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGRRYFGTD GIRGKVGDAP ITPDFVLRLG YAAGKVLASA PGRAASGARP TVLIGKDTRV SGYMLEAALE AGFSAAGVDV MLAGPMPTPG VAYLTRALRL SAGVVISASH NPYHDNGIKF FSADGNKLPD ETEAEIEAWL DKSLDCAPSD GLGKARRLDD AAGRYIEFCK STFPAAFDLR GMKLVVDCAH GAAYQVAPHV FHELGADVIP IGVAPNGFNI NDGVGATAPD ALMRAVRANH ADLGIALDGD ADRLLVVDHT GRLYNGDELL YVLVKDRIAT NGQVEGAVGT LMTNLAVEVA LKDAGVQFVR AAVGDRYVLE QLRERGWQLG AEGSGHILSL DRHSTGDGIV SALLVLAALK RSGKTLAQML EGVTLFPQKL INVRMKPGAD WKGSDAIRRA IGSAEQALAG SGRVLIRASG TEPVLRVMVE ARHAADANHH AEAIADAVKQ AT
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgggac gtcgttattt cggcacggac ggcattcgcg gcaaggtcgg cgacgcgccg atcacccccg atttcgtgtt gcggctcggt tacgcggcgg gcaaggtgct cgcgagcgcg cctggccgcg cggcgtcggg cgcgcggccg acggtgctga tcggcaagga cacgcgcgtg tcgggctaca tgctcgaagc cgcacttgaa gcaggttttt ccgcggcggg cgtcgacgtg atgctcgcgg gcccgatgcc gacgccgggc gtcgcgtatc tgacgcgcgc gctgcggctg tcggcgggcg tcgtgatcag cgcgtcgcac aatccgtatc acgacaacgg catcaagttc ttctcggccg acggcaacaa actgccggac gaaaccgaag ccgagatcga ggcgtggctc gacaagtcgc tcgattgcgc gccgtcggat gggctcggta aggcgcggcg tctcgacgac gcggccggcc gctacatcga gttctgcaag agcacgttcc cggctgcgtt cgacctgcgc gggatgaagc ttgtcgtcga ttgcgcgcac ggcgccgcgt atcaggtcgc gccgcatgta tttcacgaac ttggcgcgga tgtgatcccg atcggcgtgg cgccgaacgg cttcaacatc aacgacggcg tcggcgcgac ggcgcccgac gcgctgatgc gcgcggtgcg cgcgaaccat gccgatctgg gcattgccct cgacggcgat gcggatcgcc tgctcgtcgt cgaccatact gggcggctgt ataacggcga tgagttgctg tacgtgctcg tcaaggatcg cattgcaact aacggccagg ttgagggggc ggtgggcacg ctgatgacga atctcgccgt cgaagtcgcg ctgaaagacg cgggcgtgca gttcgtgcgg gcggcggttg gcgatcgcta cgtgctcgag cagttgcgcg agcgcggctg gcaactcggc gccgagggct cgggccacat tctgtcgctc gatcggcatt cgacgggcga cgggatcgtc tcggcgctgc tcgtgctcgc cgcgctcaag cgcagcggca agacgctcgc gcagatgctc gaaggcgtca cgctgttccc gcagaagctg atcaacgtgc ggatgaagcc tggcgccgac tggaagggca gtgatgcgat ccgccgcgcg atcggttcgg ccgagcaggc gcttgccggc agcggccgcg tgctgatccg cgcgtcgggg acggaacccg tgttgcgggt gatggtcgag gcgcggcacg cggcggatgc gaaccatcat gcggaggcga ttgccgacgc ggtcaagcag gcgacggcg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGRRYFGTD GIRGKVGDAP ITPDFVLRLG YAAGKVLASA PGRAASGARP TVLIGKDTRV SGYMLEAALE AGFSAAGVDV MLAGPMPTPG VAYLTRALRL SAGVVISASH NPYHDNGIKF FSADGNKLPD ETEAEIEAWL DKSLDCAPSD GLGKARRLDD AAGRYIEFCK STFPAAFDLR GMKLVVDCAH GAAYQVAPHV FHELGADVIP IGVAPNGFNI NDGVGATAPD ALMRAVRANH ADLGIALDGD ADRLLVVDHT GRLYNGDELL YVLVKDRIAT NGQVEGAVGT LMTNLAVEVA LKDAGVQFVR AAVGDRYVLE QLRERGWQLG AEGSGHILSL DRHSTGDGIV SALLVLAALK RSGKTLAQML EGVTLFPQKL INVRMKPGAD WKGSDAIRRA IGSAEQALAG SGRVLIRASG TEPVLRVMVE ARHAADANHH AEAIADAVKQ ATA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGGACGTCGT TATTTCGGCA CGGACGGCAT TCGCGGCAAG GTCGGCGACG CGCCGATCAC CCCCGATTTC GTGTTGCGGC TCGGTTACGC GGCGGGCAAG GTGCTCGCGA GCGCGCCTGG CCGCGCGGCG TCGGGCGCGC GGCCGACGGT GCTGATCGGC AAGGACACGC GCGTGTCGGG CTACATGCTC GAAGCCGCAC TTGAAGCAGG TTTTTCCGCG GCGGGCGTCG ACGTGATGCT CGCGGGCCCG ATGCCGACGC CGGGCGTCGC GTATCTGACG CGCGCGCTGC GGCTGTCGGC GGGCGTCGTG ATCAGCGCGT CGCACAATCC GTATCACGAC AACGGCATCA AGTTCTTCTC GGCCGACGGC AACAAACTGC CGGACGAAAC CGAAGCCGAG ATCGAGGCGT GGCTCGACAA GTCGCTCGAT TGCGCGCCGT CGGATGGGCT CGGTAAGGCG CGGCGTCTCG ACGACGCGGC CGGCCGCTAC ATCGAGTTCT GCAAGAGCAC GTTCCCGGCT GCGTTCGACC TGCGCGGGAT GAAGCTTGTC GTCGATTGCG CGCACGGCGC CGCGTATCAG GTCGCGCCGC ATGTATTTCA CGAACTTGGC GCGGATGTGA TCCCGATCGG CGTGGCGCCG AACGGCTTCA ACATCAACGA CGGCGTCGGC GCGACGGCGC CCGACGCGCT GATGCGCGCG GTGCGCGCGA ACCATGCCGA TCTGGGCATT GCCCTCGACG GCGATGCGGA TCGCCTGCTC GTCGTCGACC ATACTGGGCG GCTGTATAAC GGCGATGAGT TGCTGTACGT GCTCGTCAAG GATCGCATTG CAACTAACGG CCAGGTTGAG GGGGCGGTGG GCACGCTGAT GACGAATCTC GCCGTCGAAG TCGCGCTGAA AGACGCGGGC GTGCAGTTCG TGCGGGCGGC GGTTGGCGAT CGCTACGTGC TCGAGCAGTT GCGCGAGCGC GGCTGGCAAC TCGGCGCCGA GGGCTCGGGC CACATTCTGT CGCTCGATCG GCATTCGACG GGCGACGGGA TCGTCTCGGC GCTGCTCGTG CTCGCCGCGC TCAAGCGCAG CGGCAAGACG CTCGCGCAGA TGCTCGAAGG CGTCACGCTG TTCCCGCAGA AGCTGATCAA CGTGCGGATG AAGCCTGGCG CCGACTGGAA GGGCAGTGAT GCGATCCGCC GCGCGATCGG TTCGGCCGAG CAGGCGCTTG CCGGCAGCGG CCGCGTGCTG ATCCGCGCGT CGGGGACGGA ACCCGTGTTG CGGGTGATGG TCGAGGCGCG GCACGCGGCG GATGCGAACC ATCATGCGGA GGCGATTGCC GACGCGGTCA AGCAGGCGAC GGCGTAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA