ButhA.01370.a
Phosphoglucomutase/phosphomannomutase family protein (BTH_I1489)
| CENTER ID: | ButhA.01370.a |
| ORGANISM: | Burkholderia thailandensis E264 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.01370.a.A1.GE32764 | full length | 1 | 464 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.01370.a.A1.PS01177 | full length | 1 | 464 | ||
| ButhA.01370.a.A1.PS01178 | full length | 1 | 464 |
Structures
3UW2
DEPOSITED: 11/30/2011
DETERMINATION: XRay
CLONE: ButhA.01370.a.A1.GE32764
PROTEIN: ButhA.01370.a.A1.PS01178
Publications by SSGCID
Combining functional and structural genomics to sample the essential Burkholderia structome.
Abendroth J, Armour B, Barrett L, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Fox D, Gallagher LA, Gardberg AS, Gillespie A, Manoil C, Myler PJ, Nakazawa-Hewitt S, Napuli A, Nguyen MT, Patrapuvich R, Phan I, Stacy R, Staker BL, Stewart LJ, Van Voorhis WC
PLoS ONE - 2012
volume 8, issue 1, pages e53851
PMID: 23382856; PMCID: PMC3561365
PDB:
3D63,
3DAH,
3EIZ,
3EJ2,
3EK2,
3EZO,
3F0F,
3FTP,
3GK0,
3GK3,
3GVF,
3GWA,
3GWE,
3IML,
3SZ8,
3T4C,
3TML,
3TMQ,
3TXY,
3U7J,
3UE9,
3UK1,
3UK2,
3UND,
3UPT,
3URR,
3UW1,
3UW2,
3UW3,
3V2I,
3V7N,
3V8H,
3V9O,
3V9P,
3VAV,
4DDO,
4DFE,
4DHE,
4DHK,
4DUT,
4DZ4,
4E4T,
4E51,
4EFI,
4EG0,
4EGJ,
4EK2,
4EQY,
4EWG,
4EX5,
4EXQ,
4F2G,
4F32,
4F3N,
4F3Y,
4F4H,
4F7D,
4FK8,
4FRY,
4G1K,
4G67,
4G6Z,
4GHK,
4H3Y,
4H3Z,
4H4G
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_128112 |
| PATRIC ID: | fig|271848.6.peg.4271 |
| RefSeq: | YP_442035.1 |
| UniProt: | Q2SYG4 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MISQSIFKAY DIRGVIGKTL DADVARSIGR AFGSEVRAQG GDAVVVARDG RLSGPELVGA LADGLRAAGV DVVDVGMVPT PVGYFAASVP LALSGGERRV DSCIVVTGSH NPPDYNGFKM VLRGAAIYGD QIQGLYKRIV DARFETGSGS YEQYDVADQY VERIVGDIKL TRPLKLVVDA GNGVAGPLAT RLFKALGCEL VELFTDIDGN FPNHHPDPAH PENLQDVIAK LKATDAEIGF AFDGDGDRLG VVTKDGQIIY PDRQLMLFAE EVLSRNPGAQ IIYDVKCTRN LARWVREKGG EPLMWKTGHS LVKAKLRETG APLAGEMSGH VFFKDRWYGF DDGLYTGARL LEILARVADP SALLNGLPNA VSTPELQLKL EEGENVKLID KLRADAKFDG ADEVVTIDGL RVEYPDGFGL ARSSNTTPVV VLRFEATSDA ALARIQDDFR RALKAAKPGA NLPF
NT Sequence
atgatctccc aatccatctt caaggcatac gatattcggg gcgtgatcgg caagacgctc gacgccgacg tcgcgcgctc gatcggccgc gcattcggca gcgaagtgcg cgcgcagggc ggcgacgcgg tcgtcgtcgc gcgcgacggc cgtctgtcgg gccccgaact cgtcggcgcg ctcgcggacg ggctgcgcgc ggcgggcgtc gacgtcgtcg acgtcggcat ggtgccgacg ccggtcggct acttcgcggc gagcgtgccg ctcgcgctgt cgggcggcga gcgccgcgtc gactcgtgca tcgtcgtcac cggcagccac aatccgcccg actacaacgg cttcaagatg gtgctgcgcg gcgccgcgat ctacggcgac cagatccagg gcctgtacaa gcgcatcgtc gacgcgcgct tcgagaccgg cagcggctcg tacgagcaat acgacgttgc cgatcagtac gtcgagcgca tcgtcggcga catcaagctc acgcggccgc tgaagctcgt cgtcgacgcg ggcaacggcg tcgcgggccc gctcgcgacg cgcctgttca aggcgctcgg ctgcgagctc gtcgagctgt tcaccgacat cgacggcaac ttcccgaacc atcacccgga cccggcgcac ccggagaacc tgcaggacgt gatcgcgaag ctgaaggcga ccgacgccga gatcggcttc gcgttcgacg gcgacggcga tcgcctcggc gtcgtcacga aggacggcca gatcatctat ccggaccgcc agctgatgct gttcgccgag gaagtgctgt cgcgcaatcc gggcgcgcaa atcatctacg acgtgaagtg cacgcgcaat ctcgcgcgct gggtgcgcga gaagggcggc gagccgctga tgtggaagac gggccactcg ctcgtgaagg cgaagctgcg cgaaaccggc gcgccgctcg cgggcgagat gagcggccac gtgttcttca aggatcgctg gtacggcttc gacgacggtc tctacacggg cgcgcgcctg ctcgagatcc tcgcgcgcgt ggcggacccg agcgcgctcc tgaacggcct gccgaacgcg gtgtcgacgc ccgagcttca gctcaagctc gaagagggcg agaacgtgaa gctgatcgac aagctgcgcg ccgacgcgaa gttcgacggc gcggacgaag tcgtgacgat cgacggcctg cgcgtcgagt atccggacgg cttcggcctc gcgcgctcgt cgaacacgac gcccgtagtc gtgctgcgct tcgaggcgac gtccgacgcg gcgctcgcgc gaatccagga cgatttccgg cgcgcgctga aggcggcgaa gccgggcgcg aacctgccgt tctga
Details for ButhA.01370.a.A1.GE32764
| HARVESTED ON: | 6/20/2011 |
| SEQUENCED ON: | 7/19/2011 |
| EXPECTED MW: | 52kDa |
| OBSERVED MW: | 50kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMISQSIFKA YDIRGVIGKT LDADVARSIG RAFGSEVRAQ GGDAVVVARD GRLSGPELVG ALADGLRAAG VDVVDVGMVP TPVGYFAASV PLALSGGERR VDSCIVVTGS HNPPDYNGFK MVLRGAAIYG DQIQGLYKRI VDARFETGSG SYEQYDVADQ YVERIVGDIK LTRPLKLVVD AGNGVAGPLA TRLFKALGCE LVELFTDIDG NFPNHHPDPA HPENLQDVIA KLKATDAEIG FAFDGDGDRL GVVTKDGQII YPDRQLMLFA EEVLSRNPGA QIIYDVKCTR NLARWVREKG GEPLMWKTGH SLVKAKLRET GAPLAGEMSG HVFFKDRWYG FDDGLYTGAR LLEILARVAD PSALLNGLPN AVSTPELQLK LEEGENVKLI DKLRADAKFD GADEVVTIDG LRVEYPDGFG LARSSNTTPV VVLRFEATSD AALARIQDDF RRALKAAKPG ANLP
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgatct cccaatccat cttcaaggca tacgatattc ggggcgtgat cggcaagacg ctcgacgccg acgtcgcgcg ctcgatcggc cgcgcattcg gcagcgaagt gcgcgcgcag ggcggcgacg cggtcgtcgt cgcgcgcgac ggccgtctgt cgggccccga actcgtcggc gcgctcgcgg acgggctgcg cgcggcgggc gtcgacgtcg tcgacgtcgg catggtgccg acgccggtcg gctacttcgc ggcgagcgtg ccgctcgcgc tgtcgggcgg cgagcgccgc gtcgactcgt gcatcgtcgt caccggcagc cacaatccgc ccgactacaa cggcttcaag atggtgctgc gcggcgccgc gatctacggc gaccagatcc agggcctgta caagcgcatc gtcgacgcgc gcttcgagac cggcagcggc tcgtacgagc aatacgacgt tgccgatcag tacgtcgagc gcatcgtcgg cgacatcaag ctcacgcggc cgctgaagct cgtcgtcgac gcgggcaacg gcgtcgcggg cccgctcgcg acgcgcctgt tcaaggcgct cggctgcgag ctcgtcgagc tgttcaccga catcgacggc aacttcccga accatcaccc ggacccggcg cacccggaga acctgcagga cgtgatcgcg aagctgaagg cgaccgacgc cgagatcggc ttcgcgttcg acggcgacgg cgatcgcctc ggcgtcgtca cgaaggacgg ccagatcatc tatccggacc gccagctgat gctgttcgcc gaggaagtgc tgtcgcgcaa tccgggcgcg caaatcatct acgacgtgaa gtgcacgcgc aatctcgcgc gctgggtgcg cgagaagggc ggcgagccgc tgatgtggaa gacgggccac tcgctcgtga aggcgaagct gcgcgaaacc ggcgcgccgc tcgcgggcga gatgagcggc cacgtgttct tcaaggatcg ctggtacggc ttcgacgacg gtctctacac gggcgcgcgc ctgctcgaga tcctcgcgcg cgtggcggac ccgagcgcgc tcctgaacgg cctgccgaac gcggtgtcga cgcccgagct tcagctcaag ctcgaagagg gcgagaacgt gaagctgatc gacaagctgc gcgccgacgc gaagttcgac ggcgcggacg aagtcgtgac gatcgacggc ctgcgcgtcg agtatccgga cggcttcggc ctcgcgcgct cgtcgaacac gacgcccgta gtcgtgctgc gcttcgaggc gacgtccgac gcggcgctcg cgcgaatcca ggacgatttc cggcgcgcgc tgaaggcggc gaagccgggc gcgaacctgc cgttc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMISQSIFKA YDIRGVIGKT LDADVARSIG RAFGSEVRAQ GGDAVVVARD GRLSGPELVG ALADGLRAAG VDVVDVGMVP TPVGYFAASV PLALSGGERR VDSCIVVTGS HNPPDYNGFK MVLRGAAIYG DQIQGLYKRI VDARFETGSG SYEQYDVADQ YVERIVGDIK LTRPLKLVVD AGNGVAGPLA TRLFKALGCE LVELFTDIDG NFPNHHPDPA HPENLQDVIA KLKATDAEIG FAFDGDGDRL GVVTKDGQII YPDRQLMLFA EEVLSRNPGA QIIYDVKCTR NLARWVREKG GEPLMWKTGH SLVKAKLRET GAPLAGEMSG HVFFKDRWYG FDDGLYTGAR LLEILARVAD PSALLNGLPN AVSTPELQLK LEEGENVKLI DKLRADAKFD GADEVVTIDG LRVEYPDGFG LARSSNTTPV VVLRFEATSD AALARIQDDF RRALKAAKPG ANLPF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gatctcccaa tccatcttca aggcatacga tattcggggc gtgatcggca agacgctcga cgccgacgtc gcgcgctcga tcggccgcgc attcggcagc gaagtgcgcg cgcagggcgg cgacgcggtc gtcgtcgcgc gcgacggccg tctgtcgggc cccgaactcg tcggcgcgct cgcggacggg ctgcgcgcgg cgggcgtcga cgtcgtcgac gtcggcatgg tgccgacgcc ggtcggctac ttcgcggcga gcgtgccgct cgcgctgtcg ggcggcgagc gccgcgtcga ctcgtgcatc gtcgtcaccg gcagccacaa tccgcccgac tacaacggct tcaagatggt gctgcgcggc gccgcgatct acggcgacca gatccagggc ctgtacaagc gcatcgtcga cgcgcgcttc gagaccggca gcggctcgta cgagcaatac gacgttgccg atcagtacgt cgagcgcatc gtcggcgaca tcaagctcac gcggccgctg aagctcgtcg tcgacgcggg caacggcgtc gcgggcccgc tcgcgacgcg cctgttcaag gcgctcggct gcgagctcgt cgagctgttc accgacatcg acggcaactt cccgaaccat cacccggacc cggcgcaccc ggagaacctg caggacgtga tcgcgaagct gaaggcgacc gacgccgaga tcggcttcgc gttcgacggc gacggcgatc gcctcggcgt cgtcacgaag gacggccaga tcatctatcc ggaccgccag ctgatgctgt tcgccgagga agtgctgtcg cgcaatccgg gcgcgcaaat catctacgac gtgaagtgca cgcgcaatct cgcgcgctgg gtgcgcgaga agggcggcga gccgctgatg tggaagacgg gccactcgct cgtgaaggcg aagctgcgcg aaaccggcgc gccgctcgcg ggcgagatga gcggccacgt gttcttcaag gatcgctggt acggcttcga cgacggtctc tacacgggcg cgcgcctgct cgagatcctc gcgcgcgtgg cggacccgag cgcgctcctg aacggcctgc cgaacgcggt gtcgacgccc gagcttcagc tcaagctcga agagggcgag aacgtgaagc tgatcgacaa gctgcgcgcc gacgcgaagt tcgacggcgc ggacgaagtc gtgacgatcg acggcctgcg cgtcgagtat ccggacggct tcggcctcgc gcgctcgtcg aacacgacgc ccgtagtcgt gctgcgcttc gaggcgacgt ccgacgcggc gctcgcgcga atccaggacg atttccggcg cgcgctgaag gcggcgaagc cgggcgcgaa cctgccgttc taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for ButhA.01370.a.A1.PS01177
| PURIFICATION DATe: | 8/24/2011 |
| CONCENTRATION: | 61.97mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMISQSIFKA YDIRGVIGKT LDADVARSIG RAFGSEVRAQ GGDAVVVARD GRLSGPELVG ALADGLRAAG VDVVDVGMVP TPVGYFAASV PLALSGGERR VDSCIVVTGS HNPPDYNGFK MVLRGAAIYG DQIQGLYKRI VDARFETGSG SYEQYDVADQ YVERIVGDIK LTRPLKLVVD AGNGVAGPLA TRLFKALGCE LVELFTDIDG NFPNHHPDPA HPENLQDVIA KLKATDAEIG FAFDGDGDRL GVVTKDGQII YPDRQLMLFA EEVLSRNPGA QIIYDVKCTR NLARWVREKG GEPLMWKTGH SLVKAKLRET GAPLAGEMSG HVFFKDRWYG FDDGLYTGAR LLEILARVAD PSALLNGLPN AVSTPELQLK LEEGENVKLI DKLRADAKFD GADEVVTIDG LRVEYPDGFG LARSSNTTPV VVLRFEATSD AALARIQDDF RRALKAAKPG ANLP
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgatct cccaatccat cttcaaggca tacgatattc ggggcgtgat cggcaagacg ctcgacgccg acgtcgcgcg ctcgatcggc cgcgcattcg gcagcgaagt gcgcgcgcag ggcggcgacg cggtcgtcgt cgcgcgcgac ggccgtctgt cgggccccga actcgtcggc gcgctcgcgg acgggctgcg cgcggcgggc gtcgacgtcg tcgacgtcgg catggtgccg acgccggtcg gctacttcgc ggcgagcgtg ccgctcgcgc tgtcgggcgg cgagcgccgc gtcgactcgt gcatcgtcgt caccggcagc cacaatccgc ccgactacaa cggcttcaag atggtgctgc gcggcgccgc gatctacggc gaccagatcc agggcctgta caagcgcatc gtcgacgcgc gcttcgagac cggcagcggc tcgtacgagc aatacgacgt tgccgatcag tacgtcgagc gcatcgtcgg cgacatcaag ctcacgcggc cgctgaagct cgtcgtcgac gcgggcaacg gcgtcgcggg cccgctcgcg acgcgcctgt tcaaggcgct cggctgcgag ctcgtcgagc tgttcaccga catcgacggc aacttcccga accatcaccc ggacccggcg cacccggaga acctgcagga cgtgatcgcg aagctgaagg cgaccgacgc cgagatcggc ttcgcgttcg acggcgacgg cgatcgcctc ggcgtcgtca cgaaggacgg ccagatcatc tatccggacc gccagctgat gctgttcgcc gaggaagtgc tgtcgcgcaa tccgggcgcg caaatcatct acgacgtgaa gtgcacgcgc aatctcgcgc gctgggtgcg cgagaagggc ggcgagccgc tgatgtggaa gacgggccac tcgctcgtga aggcgaagct gcgcgaaacc ggcgcgccgc tcgcgggcga gatgagcggc cacgtgttct tcaaggatcg ctggtacggc ttcgacgacg gtctctacac gggcgcgcgc ctgctcgaga tcctcgcgcg cgtggcggac ccgagcgcgc tcctgaacgg cctgccgaac gcggtgtcga cgcccgagct tcagctcaag ctcgaagagg gcgagaacgt gaagctgatc gacaagctgc gcgccgacgc gaagttcgac ggcgcggacg aagtcgtgac gatcgacggc ctgcgcgtcg agtatccgga cggcttcggc ctcgcgcgct cgtcgaacac gacgcccgta gtcgtgctgc gcttcgaggc gacgtccgac gcggcgctcg cgcgaatcca ggacgatttc cggcgcgcgc tgaaggcggc gaagccgggc gcgaacctgc cgttc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMISQSIFKA YDIRGVIGKT LDADVARSIG RAFGSEVRAQ GGDAVVVARD GRLSGPELVG ALADGLRAAG VDVVDVGMVP TPVGYFAASV PLALSGGERR VDSCIVVTGS HNPPDYNGFK MVLRGAAIYG DQIQGLYKRI VDARFETGSG SYEQYDVADQ YVERIVGDIK LTRPLKLVVD AGNGVAGPLA TRLFKALGCE LVELFTDIDG NFPNHHPDPA HPENLQDVIA KLKATDAEIG FAFDGDGDRL GVVTKDGQII YPDRQLMLFA EEVLSRNPGA QIIYDVKCTR NLARWVREKG GEPLMWKTGH SLVKAKLRET GAPLAGEMSG HVFFKDRWYG FDDGLYTGAR LLEILARVAD PSALLNGLPN AVSTPELQLK LEEGENVKLI DKLRADAKFD GADEVVTIDG LRVEYPDGFG LARSSNTTPV VVLRFEATSD AALARIQDDF RRALKAAKPG ANLPF
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GATCTCCCAA TCCATCTTCA AGGCATACGA TATTCGGGGC GTGATCGGCA AGACGCTCGA CGCCGACGTC GCGCGCTCGA TCGGCCGCGC ATTCGGCAGC GAAGTGCGCG CGCAGGGCGG CGACGCGGTC GTCGTCGCGC GCGACGGCCG TCTGTCGGGC CCCGAACTCG TCGGCGCGCT CGCGGACGGG CTGCGCGCGG CGGGCGTCGA CGTCGTCGAC GTCGGCATGG TGCCGACGCC GGTCGGCTAC TTCGCGGCGA GCGTGCCGCT CGCGCTGTCG GGCGGCGAGC GCCGCGTCGA CTCGTGCATC GTCGTCACCG GCAGCCACAA TCCGCCCGAC TACAACGGCT TCAAGATGGT GCTGCGCGGC GCCGCGATCT ACGGCGACCA GATCCAGGGC CTGTACAAGC GCATCGTCGA CGCGCGCTTC GAGACCGGCA GCGGCTCGTA CGAGCAATAC GACGTTGCCG ATCAGTACGT CGAGCGCATC GTCGGCGACA TCAAGCTCAC GCGGCCGCTG AAGCTCGTCG TCGACGCGGG CAACGGCGTC GCGGGCCCGC TCGCGACGCG CCTGTTCAAG GCGCTCGGCT GCGAGCTCGT CGAGCTGTTC ACCGACATCG ACGGCAACTT CCCGAACCAT CACCCGGACC CGGCGCACCC GGAGAACCTG CAGGACGTGA TCGCGAAGCT GAAGGCGACC GACGCCGAGA TCGGCTTCGC GTTCGACGGC GACGGCGATC GCCTCGGCGT CGTCACGAAG GACGGCCAGA TCATCTATCC GGACCGCCAG CTGATGCTGT TCGCCGAGGA AGTGCTGTCG CGCAATCCGG GCGCGCAAAT CATCTACGAC GTGAAGTGCA CGCGCAATCT CGCGCGCTGG GTGCGCGAGA AGGGCGGCGA GCCGCTGATG TGGAAGACGG GCCACTCGCT CGTGAAGGCG AAGCTGCGCG AAACCGGCGC GCCGCTCGCG GGCGAGATGA GCGGCCACGT GTTCTTCAAG GATCGCTGGT ACGGCTTCGA CGACGGTCTC TACACGGGCG CGCGCCTGCT CGAGATCCTC GCGCGCGTGG CGGACCCGAG CGCGCTCCTG AACGGCCTGC CGAACGCGGT GTCGACGCCC GAGCTTCAGC TCAAGCTCGA AGAGGGCGAG AACGTGAAGC TGATCGACAA GCTGCGCGCC GACGCGAAGT TCGACGGCGC GGACGAAGTC GTGACGATCG ACGGCCTGCG CGTCGAGTAT CCGGACGGCT TCGGCCTCGC GCGCTCGTCG AACACGACGC CCGTAGTCGT GCTGCGCTTC GAGGCGACGT CCGACGCGGC GCTCGCGCGA ATCCAGGACG ATTTCCGGCG CGCGCTGAAG GCGGCGAAGC CGGGCGCGAA CCTGCCGTTC TAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA
Details for ButhA.01370.a.A1.PS01178
| PURIFICATION DATe: | 8/24/2011 |
| CONCENTRATION: | 32.43mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMISQSIFKA YDIRGVIGKT LDADVARSIG RAFGSEVRAQ GGDAVVVARD GRLSGPELVG ALADGLRAAG VDVVDVGMVP TPVGYFAASV PLALSGGERR VDSCIVVTGS HNPPDYNGFK MVLRGAAIYG DQIQGLYKRI VDARFETGSG SYEQYDVADQ YVERIVGDIK LTRPLKLVVD AGNGVAGPLA TRLFKALGCE LVELFTDIDG NFPNHHPDPA HPENLQDVIA KLKATDAEIG FAFDGDGDRL GVVTKDGQII YPDRQLMLFA EEVLSRNPGA QIIYDVKCTR NLARWVREKG GEPLMWKTGH SLVKAKLRET GAPLAGEMSG HVFFKDRWYG FDDGLYTGAR LLEILARVAD PSALLNGLPN AVSTPELQLK LEEGENVKLI DKLRADAKFD GADEVVTIDG LRVEYPDGFG LARSSNTTPV VVLRFEATSD AALARIQDDF RRALKAAKPG ANLP
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgatct cccaatccat cttcaaggca tacgatattc ggggcgtgat cggcaagacg ctcgacgccg acgtcgcgcg ctcgatcggc cgcgcattcg gcagcgaagt gcgcgcgcag ggcggcgacg cggtcgtcgt cgcgcgcgac ggccgtctgt cgggccccga actcgtcggc gcgctcgcgg acgggctgcg cgcggcgggc gtcgacgtcg tcgacgtcgg catggtgccg acgccggtcg gctacttcgc ggcgagcgtg ccgctcgcgc tgtcgggcgg cgagcgccgc gtcgactcgt gcatcgtcgt caccggcagc cacaatccgc ccgactacaa cggcttcaag atggtgctgc gcggcgccgc gatctacggc gaccagatcc agggcctgta caagcgcatc gtcgacgcgc gcttcgagac cggcagcggc tcgtacgagc aatacgacgt tgccgatcag tacgtcgagc gcatcgtcgg cgacatcaag ctcacgcggc cgctgaagct cgtcgtcgac gcgggcaacg gcgtcgcggg cccgctcgcg acgcgcctgt tcaaggcgct cggctgcgag ctcgtcgagc tgttcaccga catcgacggc aacttcccga accatcaccc ggacccggcg cacccggaga acctgcagga cgtgatcgcg aagctgaagg cgaccgacgc cgagatcggc ttcgcgttcg acggcgacgg cgatcgcctc ggcgtcgtca cgaaggacgg ccagatcatc tatccggacc gccagctgat gctgttcgcc gaggaagtgc tgtcgcgcaa tccgggcgcg caaatcatct acgacgtgaa gtgcacgcgc aatctcgcgc gctgggtgcg cgagaagggc ggcgagccgc tgatgtggaa gacgggccac tcgctcgtga aggcgaagct gcgcgaaacc ggcgcgccgc tcgcgggcga gatgagcggc cacgtgttct tcaaggatcg ctggtacggc ttcgacgacg gtctctacac gggcgcgcgc ctgctcgaga tcctcgcgcg cgtggcggac ccgagcgcgc tcctgaacgg cctgccgaac gcggtgtcga cgcccgagct tcagctcaag ctcgaagagg gcgagaacgt gaagctgatc gacaagctgc gcgccgacgc gaagttcgac ggcgcggacg aagtcgtgac gatcgacggc ctgcgcgtcg agtatccgga cggcttcggc ctcgcgcgct cgtcgaacac gacgcccgta gtcgtgctgc gcttcgaggc gacgtccgac gcggcgctcg cgcgaatcca ggacgatttc cggcgcgcgc tgaaggcggc gaagccgggc gcgaacctgc cgttc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMISQSIFKA YDIRGVIGKT LDADVARSIG RAFGSEVRAQ GGDAVVVARD GRLSGPELVG ALADGLRAAG VDVVDVGMVP TPVGYFAASV PLALSGGERR VDSCIVVTGS HNPPDYNGFK MVLRGAAIYG DQIQGLYKRI VDARFETGSG SYEQYDVADQ YVERIVGDIK LTRPLKLVVD AGNGVAGPLA TRLFKALGCE LVELFTDIDG NFPNHHPDPA HPENLQDVIA KLKATDAEIG FAFDGDGDRL GVVTKDGQII YPDRQLMLFA EEVLSRNPGA QIIYDVKCTR NLARWVREKG GEPLMWKTGH SLVKAKLRET GAPLAGEMSG HVFFKDRWYG FDDGLYTGAR LLEILARVAD PSALLNGLPN AVSTPELQLK LEEGENVKLI DKLRADAKFD GADEVVTIDG LRVEYPDGFG LARSSNTTPV VVLRFEATSD AALARIQDDF RRALKAAKPG ANLPF
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GATCTCCCAA TCCATCTTCA AGGCATACGA TATTCGGGGC GTGATCGGCA AGACGCTCGA CGCCGACGTC GCGCGCTCGA TCGGCCGCGC ATTCGGCAGC GAAGTGCGCG CGCAGGGCGG CGACGCGGTC GTCGTCGCGC GCGACGGCCG TCTGTCGGGC CCCGAACTCG TCGGCGCGCT CGCGGACGGG CTGCGCGCGG CGGGCGTCGA CGTCGTCGAC GTCGGCATGG TGCCGACGCC GGTCGGCTAC TTCGCGGCGA GCGTGCCGCT CGCGCTGTCG GGCGGCGAGC GCCGCGTCGA CTCGTGCATC GTCGTCACCG GCAGCCACAA TCCGCCCGAC TACAACGGCT TCAAGATGGT GCTGCGCGGC GCCGCGATCT ACGGCGACCA GATCCAGGGC CTGTACAAGC GCATCGTCGA CGCGCGCTTC GAGACCGGCA GCGGCTCGTA CGAGCAATAC GACGTTGCCG ATCAGTACGT CGAGCGCATC GTCGGCGACA TCAAGCTCAC GCGGCCGCTG AAGCTCGTCG TCGACGCGGG CAACGGCGTC GCGGGCCCGC TCGCGACGCG CCTGTTCAAG GCGCTCGGCT GCGAGCTCGT CGAGCTGTTC ACCGACATCG ACGGCAACTT CCCGAACCAT CACCCGGACC CGGCGCACCC GGAGAACCTG CAGGACGTGA TCGCGAAGCT GAAGGCGACC GACGCCGAGA TCGGCTTCGC GTTCGACGGC GACGGCGATC GCCTCGGCGT CGTCACGAAG GACGGCCAGA TCATCTATCC GGACCGCCAG CTGATGCTGT TCGCCGAGGA AGTGCTGTCG CGCAATCCGG GCGCGCAAAT CATCTACGAC GTGAAGTGCA CGCGCAATCT CGCGCGCTGG GTGCGCGAGA AGGGCGGCGA GCCGCTGATG TGGAAGACGG GCCACTCGCT CGTGAAGGCG AAGCTGCGCG AAACCGGCGC GCCGCTCGCG GGCGAGATGA GCGGCCACGT GTTCTTCAAG GATCGCTGGT ACGGCTTCGA CGACGGTCTC TACACGGGCG CGCGCCTGCT CGAGATCCTC GCGCGCGTGG CGGACCCGAG CGCGCTCCTG AACGGCCTGC CGAACGCGGT GTCGACGCCC GAGCTTCAGC TCAAGCTCGA AGAGGGCGAG AACGTGAAGC TGATCGACAA GCTGCGCGCC GACGCGAAGT TCGACGGCGC GGACGAAGTC GTGACGATCG ACGGCCTGCG CGTCGAGTAT CCGGACGGCT TCGGCCTCGC GCGCTCGTCG AACACGACGC CCGTAGTCGT GCTGCGCTTC GAGGCGACGT CCGACGCGGC GCTCGCGCGA ATCCAGGACG ATTTCCGGCG CGCGCTGAAG GCGGCGAAGC CGGGCGCGAA CCTGCCGTTC TAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA