ButhA.01294.a
Transketolase (EC 2.2.1.1) (BTH_I1195) (tkt)
CENTER ID:
ButhA.01294.a
ORGANISM:
Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:
in PDB
COMMUNITY REQUEST:
True
NIH RISK GROUP:
3
SELECT AGENT:
False
NIH PRIORITY
pathogens category:
IIIB
Ordering Clones & Proteins
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Clones*
CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO
AA
START
AA
STOP
ORDER
MATERIAL
ButhA.01294.a.A1.GE32762
full length
info
1
690
ButhA.01294.a.A1.GE32762
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis . Get sequence information
using the button in the "info" column.
Proteins
CENTER REFERENCE ID
DOMAIN/REGION DESCRIPTION
INFO
AA START
AA STOP
ORDER MATERIAL
ButhA.01294.a.A1.PS01189
full length
info
1
690
PS01189
Structures
3UK1
DEPOSITED: 11/8/2011
DETERMINATION: XRay
CLONE: ButhA.01294.a.A1.GE32762
PROTEIN: ButhA.01294.a.A1.PS01189
3UPT
DEPOSITED: 11/18/2011
DETERMINATION: XRay
CLONE: ButhA.01294.a.A1.GE32762
PROTEIN: ButhA.01294.a.A1.PS01189
Publications by SSGCID
Combining functional and structural genomics to sample the essential Burkholderia structome.
Abendroth J, Armour B, Barrett L, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Fox D, Gallagher LA, Gardberg AS, Gillespie A, Manoil C, Myler PJ, Nakazawa-Hewitt S, Napuli A, Nguyen MT, Patrapuvich R, Phan I, Stacy R, Staker BL, Stewart LJ, Van Voorhis WC
PLoS ONE - 2012
volume 8, issue 1, pages e53851
PDB:
3D63 ,
3DAH ,
3EIZ ,
3EJ2 ,
3EK2 ,
3EZO ,
3F0F ,
3FTP ,
3GK0 ,
3GK3 ,
3GVF ,
3GWA ,
3GWE ,
3IML ,
3SZ8 ,
3T4C ,
3TML ,
3TMQ ,
3TXY ,
3U7J ,
3UE9 ,
3UK1 ,
3UK2 ,
3UND ,
3UPT ,
3URR ,
3UW1 ,
3UW2 ,
3UW3 ,
3V2I ,
3V7N ,
3V8H ,
3V9O ,
3V9P ,
3VAV ,
4DDO ,
4DFE ,
4DHE ,
4DHK ,
4DUT ,
4DZ4 ,
4E4T ,
4E51 ,
4EFI ,
4EG0 ,
4EGJ ,
4EK2 ,
4EQY ,
4EWG ,
4EX5 ,
4EXQ ,
4F2G ,
4F32 ,
4F3N ,
4F3Y ,
4F4H ,
4F7D ,
4FK8 ,
4FRY ,
4G1K ,
4G67 ,
4G6Z ,
4GHK ,
4H3Y ,
4H3Z ,
4H4G
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPPVPRFLDS FSGLDMTTSS PASTTLMANA IRALAMDAVQ QANSGHPGMP MGMAEIGVAL WSRHLKHNPT NPHWADRDRF VLSNGHGSML LYSLLHLTGY DLPIEELKNF RQLHSKTPGH PEYGITPGVE TTTGPLGQGL ANAVGMALGE ALLAAEFNRD DAKIVDHHTY VFLGDGCLME GISHEACSLA GTLKLNKLIA LYDDNGISID GDVVNWFHDD TPKRFEAYGW NVIPNVNGHD VDAIDAAIAK AKRSDKPSLI CCKTRIGNGA ATKAGGHDVH GAPLGADEIA KTREALGWTW APFVIPQEVY AAWDAKEAGK RSEDDWNAAF AQYRAKYPAE AAEFERRMAG TLPADWAAKA AAIVAGANER GETVATRKAS QQTIEGLAAV LPELLGGSAD LTGSNLTNWK ASKAVRANAD GPGVQWGNHI NYGVREFGMS AAINGLVLHG GYKPFGGTFL TFSDYSRNAL RVAALMKVPS IFVFTHDSIG LGEDGPTHQS VEHVASLRLI PNLDVWRPAD TVETAVAWTY AVAHQHPSCL IFSRQNLAFN ARTDAQLANV EKGGYVLRDW DEEIVARKII LIATGSEVEL AMKAVEPLAQ QGIAARVVSM PSSDVFDRQD AEYRERVLPH GVRRVAIEAG VTDFWRKYVG LEGGVVGIDT FGESAPAGVL FKHFGFTVEH VIETAKAVLA
NT Sequence
atgccgcccg tgccccgttt tctcgactcg ttctccggac tcgacatgac gacttcgtct cccgcctcca ccaccttgat ggccaacgcg atccgcgcgc tcgcgatgga cgccgtccag caagcgaact ccggtcaccc cggcatgccg atgggcatgg ccgagatcgg cgtcgcgctg tggtcgcgcc acctgaagca caacccgacg aacccgcatt gggccgaccg cgaccgcttc gtgctgtcga acggccacgg ctcgatgctg ctgtactcgc tgctgcacct gacaggctac gatctgccga tcgaagagct gaagaatttc cggcaactgc attcgaagac gccgggccac ccggaatacg ggatcacgcc gggcgtcgag acgacgacgg gcccgctcgg ccagggcctc gcgaacgcgg tcggcatggc gctcggcgag gcgctgctcg ccgccgagtt caaccgcgac gacgcgaaga tcgtcgatca ccacacgtac gtgttcctcg gcgacggctg cctgatggaa ggcatctcgc acgaggcctg ctcgctcgcg ggcacgctga agctcaacaa gctgatcgcg ctctatgacg acaacgggat ctcgatcgac ggcgacgtcg tcaactggtt ccacgacgac acgccgaagc gcttcgaggc gtacggctgg aacgtgattc cgaacgtgaa cggccatgac gtcgacgcga tcgacgcggc gatcgcgaag gcgaagcgct cggacaagcc gagcctcatc tgctgcaaga cgcgcatcgg caacggcgcg gcgacgaagg cgggcggcca cgacgtgcac ggcgcgccgc tcggcgcgga cgaaatcgcg aagacccgcg aggcgctcgg ctggacgtgg gcgccgttcg tgattccgca ggaagtctat gcggcatggg acgcgaagga ggcgggcaag cgcagcgaag acgactggaa cgcggcgttc gcgcaatacc gcgcgaagta cccggccgaa gcggccgaat tcgagcgccg gatggcgggc acgctgccgg ccgattgggc cgcgaaggcc gcggcgatcg tcgccggcgc gaacgagcgc ggcgagacgg tcgcgacgcg caaggcgtcg cagcagacga tcgaggggct cgccgccgtg ctgcccgagc tgctcggcgg ctcggcggac ctgacgggct cgaacctcac gaactggaag gcgtcgaagg cggttcgcgc gaatgcggac ggcccgggcg tccagtgggg caaccatatc aactacggcg tgcgcgaatt cggcatgagc gccgcgatca acggcctcgt gctgcacggc ggctacaagc cgttcggcgg cacgttcctc acgttctccg actacagccg caatgcgctg cgcgtcgccg cgctgatgaa ggtgccgtcg atcttcgtgt tcacgcacga ttcgatcggc ctcggcgagg acggcccgac gcaccagtcg gtcgagcatg tcgcgagcct gcgcctgatt cccaacctcg acgtctggcg tccggcggac acggtggaga cggccgtcgc gtggacctac gcggtcgcgc accagcaccc gtcgtgcctg atcttcagcc gccagaacct cgcgttcaac gcgcgcaccg atgcgcagct cgcgaacgtc gagaagggcg gctacgtgct gcgcgactgg gacgaagaaa tcgtcgcgcg caagatcatc ctgatcgcga cgggctccga agtcgagctc gcgatgaagg cggtcgagcc cctcgcgcag caggggatcg ccgcgcgcgt cgtgtcgatg ccgtcgagcg acgtgttcga ccgccaggac gccgagtacc gcgagcgggt gctgccgcac ggcgtgcgcc gcgtcgcgat cgaagcgggc gtcaccgatt tctggcgcaa gtacgtgggc ctcgaaggcg gcgtggtcgg catcgacacg ttcggcgagt cggccccggc cggcgtgctg ttcaagcatt tcggcttcac tgtcgagcac gtgatcgaaa cggcgaaggc ggtgctcgcg taa
Details for ButhA.01294.a.A1.GE32762
HARVESTED ON:
6/20/2011
SEQUENCED ON:
7/19/2011
EXPECTED MW:
76kDa
OBSERVED MW:
77kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Too many to count (100+)
TOTAL EXPRESSION LEVEL:
High Expression
SOLUBLE EXPRESSION LEVEL
High Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
pass with incomplete coverage
PERCENT IDENTITY:
84
PERCENT COVERAGE:
29
Details for ButhA.01294.a.A1.PS01189
PURIFICATION DATe:
8/17/2011
CONCENTRATION:
44.4mg/ml
OBSERVED MW:
data unavailable
EXPRESSION LEVEL:
High Expression
PROTEIN PURIFICATION BUFFER:
20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
1
VIAL VOLUME:
200µl
PERCENT IDENTITY:
84
PERCENT COVERAGE:
29