ButhA.01294.a

Transketolase (EC 2.2.1.1) (BTH_I1195) (tkt)

CENTER ID: ButhA.01294.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: in PDB
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
ButhA.01294.a.A1.GE32762 full length 1 690
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
ButhA.01294.a.A1.PS01189 full length 1 690

Structures

3UK1
DEPOSITED: 11/8/2011
DETERMINATION: XRay
CLONE: ButhA.01294.a.A1.GE32762
PROTEIN: ButhA.01294.a.A1.PS01189
3UPT
DEPOSITED: 11/18/2011
DETERMINATION: XRay
CLONE: ButhA.01294.a.A1.GE32762
PROTEIN: ButhA.01294.a.A1.PS01189

Publications by SSGCID

Combining functional and structural genomics to sample the essential Burkholderia structome.
Abendroth J, Armour B, Barrett L, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Fox D, Gallagher LA, Gardberg AS, Gillespie A, Manoil C, Myler PJ, Nakazawa-Hewitt S, Napuli A, Nguyen MT, Patrapuvich R, Phan I, Stacy R, Staker BL, Stewart LJ, Van Voorhis WC
PLoS ONE - 2012
volume 8, issue 1, pages e53851
PMID: 23382856; PMCID: PMC3561365

External Resources

RESOURCE REFERENCE ID
OrthoMCL: OG5_126720
PATRIC ID: fig|271848.6.peg.3951
RefSeq: YP_441742.1
UniProt: Q2SZA7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPPVPRFLDS FSGLDMTTSS PASTTLMANA IRALAMDAVQ QANSGHPGMP MGMAEIGVAL WSRHLKHNPT NPHWADRDRF VLSNGHGSML LYSLLHLTGY DLPIEELKNF RQLHSKTPGH PEYGITPGVE TTTGPLGQGL ANAVGMALGE ALLAAEFNRD DAKIVDHHTY VFLGDGCLME GISHEACSLA GTLKLNKLIA LYDDNGISID GDVVNWFHDD TPKRFEAYGW NVIPNVNGHD VDAIDAAIAK AKRSDKPSLI CCKTRIGNGA ATKAGGHDVH GAPLGADEIA KTREALGWTW APFVIPQEVY AAWDAKEAGK RSEDDWNAAF AQYRAKYPAE AAEFERRMAG TLPADWAAKA AAIVAGANER GETVATRKAS QQTIEGLAAV LPELLGGSAD LTGSNLTNWK ASKAVRANAD GPGVQWGNHI NYGVREFGMS AAINGLVLHG GYKPFGGTFL TFSDYSRNAL RVAALMKVPS IFVFTHDSIG LGEDGPTHQS VEHVASLRLI PNLDVWRPAD TVETAVAWTY AVAHQHPSCL IFSRQNLAFN ARTDAQLANV EKGGYVLRDW DEEIVARKII LIATGSEVEL AMKAVEPLAQ QGIAARVVSM PSSDVFDRQD AEYRERVLPH GVRRVAIEAG VTDFWRKYVG LEGGVVGIDT FGESAPAGVL FKHFGFTVEH VIETAKAVLA
NT Sequence
atgccgcccg tgccccgttt tctcgactcg ttctccggac tcgacatgac gacttcgtct cccgcctcca ccaccttgat ggccaacgcg atccgcgcgc tcgcgatgga cgccgtccag caagcgaact ccggtcaccc cggcatgccg atgggcatgg ccgagatcgg cgtcgcgctg tggtcgcgcc acctgaagca caacccgacg aacccgcatt gggccgaccg cgaccgcttc gtgctgtcga acggccacgg ctcgatgctg ctgtactcgc tgctgcacct gacaggctac gatctgccga tcgaagagct gaagaatttc cggcaactgc attcgaagac gccgggccac ccggaatacg ggatcacgcc gggcgtcgag acgacgacgg gcccgctcgg ccagggcctc gcgaacgcgg tcggcatggc gctcggcgag gcgctgctcg ccgccgagtt caaccgcgac gacgcgaaga tcgtcgatca ccacacgtac gtgttcctcg gcgacggctg cctgatggaa ggcatctcgc acgaggcctg ctcgctcgcg ggcacgctga agctcaacaa gctgatcgcg ctctatgacg acaacgggat ctcgatcgac ggcgacgtcg tcaactggtt ccacgacgac acgccgaagc gcttcgaggc gtacggctgg aacgtgattc cgaacgtgaa cggccatgac gtcgacgcga tcgacgcggc gatcgcgaag gcgaagcgct cggacaagcc gagcctcatc tgctgcaaga cgcgcatcgg caacggcgcg gcgacgaagg cgggcggcca cgacgtgcac ggcgcgccgc tcggcgcgga cgaaatcgcg aagacccgcg aggcgctcgg ctggacgtgg gcgccgttcg tgattccgca ggaagtctat gcggcatggg acgcgaagga ggcgggcaag cgcagcgaag acgactggaa cgcggcgttc gcgcaatacc gcgcgaagta cccggccgaa gcggccgaat tcgagcgccg gatggcgggc acgctgccgg ccgattgggc cgcgaaggcc gcggcgatcg tcgccggcgc gaacgagcgc ggcgagacgg tcgcgacgcg caaggcgtcg cagcagacga tcgaggggct cgccgccgtg ctgcccgagc tgctcggcgg ctcggcggac ctgacgggct cgaacctcac gaactggaag gcgtcgaagg cggttcgcgc gaatgcggac ggcccgggcg tccagtgggg caaccatatc aactacggcg tgcgcgaatt cggcatgagc gccgcgatca acggcctcgt gctgcacggc ggctacaagc cgttcggcgg cacgttcctc acgttctccg actacagccg caatgcgctg cgcgtcgccg cgctgatgaa ggtgccgtcg atcttcgtgt tcacgcacga ttcgatcggc ctcggcgagg acggcccgac gcaccagtcg gtcgagcatg tcgcgagcct gcgcctgatt cccaacctcg acgtctggcg tccggcggac acggtggaga cggccgtcgc gtggacctac gcggtcgcgc accagcaccc gtcgtgcctg atcttcagcc gccagaacct cgcgttcaac gcgcgcaccg atgcgcagct cgcgaacgtc gagaagggcg gctacgtgct gcgcgactgg gacgaagaaa tcgtcgcgcg caagatcatc ctgatcgcga cgggctccga agtcgagctc gcgatgaagg cggtcgagcc cctcgcgcag caggggatcg ccgcgcgcgt cgtgtcgatg ccgtcgagcg acgtgttcga ccgccaggac gccgagtacc gcgagcgggt gctgccgcac ggcgtgcgcc gcgtcgcgat cgaagcgggc gtcaccgatt tctggcgcaa gtacgtgggc ctcgaaggcg gcgtggtcgg catcgacacg ttcggcgagt cggccccggc cggcgtgctg ttcaagcatt tcggcttcac tgtcgagcac gtgatcgaaa cggcgaaggc ggtgctcgcg taa
Details for ButhA.01294.a.A1.GE32762
HARVESTED ON: 6/20/2011
SEQUENCED ON: 7/19/2011
EXPECTED MW: 76kDa
OBSERVED MW: 77kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 84
PERCENT COVERAGE: 29
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPPVPRFLD SFSGLDMTTS SPASTTLMAN AIRALAMDAV QQANSGHPGM PMGMAXIGVA LWSRHLKHNP TNPHWADRXR FVLSNGHGSM LLYSLLXLXX YDLPIEELXN FRQLHSKTPG HPEYGITXGV XXXTXXLGQG LANAVGMALG EALLAXXFNR DXXXIXXHHT YVXPXXXXPD XXXLARXLLA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgccgc ccgtgccccg ttttctcgac tcgttctccg gactcgacat gacgacttcg tctcccgcct ccaccacctt gatggccaac gcgatccgcg cgctcgcgat ggacgccgtc cagcaagcga actccggtca ccccggcatg ccgatgggca tggccnanat cggcgtcgcg ctgtggtcgc gccacctgaa gcacaacccg acgaacccgc attgggccga ccgnnnccgc ttcgtgctgt cgaacggcca cggctcgatg ctgctgtact cgctgctgcn cctgannngn tacgatctgc cgatcgaaga gctgaanaat ttccggcaac tgcattcgaa gacgccgggc cacccggaat acgggatcac gnnnggcgtn nngannncga cngnnncgct cggccagggc ctcgcgaacg cggtcggcat ggcgctcggc gaggcgctgc tcgccnncna gttcaaccgc gacgannnnn nnatcgncna tcaccacacg tacgtgnttc ctcnnnnncn nntgcctgat gnaangnntc tcgcacgann nctgctcgct c
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPPVPRFLD SFSGLDMTTS SPASTTLMAN AIRALAMDAV QQANSGHPGM PMGMAEIGVA LWSRHLKHNP TNPHWADRDR FVLSNGHGSM LLYSLLHLTG YDLPIEELKN FRQLHSKTPG HPEYGITPGV ETTTGPLGQG LANAVGMALG EALLAAEFNR DDAKIVDHHT YVFLGDGCLM EGISHEACSL AGTLKLNKLI ALYDDNGISI DGDVVNWFHD DTPKRFEAYG WNVIPNVNGH DVDAIDAAIA KAKRSDKPSL ICCKTRIGNG AATKAGGHDV HGAPLGADEI AKTREALGWT WAPFVIPQEV YAAWDAKEAG KRSEDDWNAA FAQYRAKYPA EAAEFERRMA GTLPADWAAK AAAIVAGANE RGETVATRKA SQQTIEGLAA VLPELLGGSA DLTGSNLTNW KASKAVRANA DGPGVQWGNH INYGVREFGM SAAINGLVLH GGYKPFGGTF LTFSDYSRNA LRVAALMKVP SIFVFTHDSI GLGEDGPTHQ SVEHVASLRL IPNLDVWRPA DTVETAVAWT YAVAHQHPSC LIFSRQNLAF NARTDAQLAN VEKGGYVLRD WDEEIVARKI ILIATGSEVE LAMKAVEPLA QQGIAARVVS MPSSDVFDRQ DAEYRERVLP HGVRRVAIEA GVTDFWRKYV GLEGGVVGID TFGESAPAGV LFKHFGFTVE HVIETAKAVL A
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccgcccgtg ccccgttttc tcgactcgtt ctccggactc gacatgacga cttcgtctcc cgcctccacc accttgatgg ccaacgcgat ccgcgcgctc gcgatggacg ccgtccagca agcgaactcc ggtcaccccg gcatgccgat gggcatggcc gagatcggcg tcgcgctgtg gtcgcgccac ctgaagcaca acccgacgaa cccgcattgg gccgaccgcg accgcttcgt gctgtcgaac ggccacggct cgatgctgct gtactcgctg ctgcacctga caggctacga tctgccgatc gaagagctga agaatttccg gcaactgcat tcgaagacgc cgggccaccc ggaatacggg atcacgccgg gcgtcgagac gacgacgggc ccgctcggcc agggcctcgc gaacgcggtc ggcatggcgc tcggcgaggc gctgctcgcc gccgagttca accgcgacga cgcgaagatc gtcgatcacc acacgtacgt gttcctcggc gacggctgcc tgatggaagg catctcgcac gaggcctgct cgctcgcggg cacgctgaag ctcaacaagc tgatcgcgct ctatgacgac aacgggatct cgatcgacgg cgacgtcgtc aactggttcc acgacgacac gccgaagcgc ttcgaggcgt acggctggaa cgtgattccg aacgtgaacg gccatgacgt cgacgcgatc gacgcggcga tcgcgaaggc gaagcgctcg gacaagccga gcctcatctg ctgcaagacg cgcatcggca acggcgcggc gacgaaggcg ggcggccacg acgtgcacgg cgcgccgctc ggcgcggacg aaatcgcgaa gacccgcgag gcgctcggct ggacgtgggc gccgttcgtg attccgcagg aagtctatgc ggcatgggac gcgaaggagg cgggcaagcg cagcgaagac gactggaacg cggcgttcgc gcaataccgc gcgaagtacc cggccgaagc ggccgaattc gagcgccgga tggcgggcac gctgccggcc gattgggccg cgaaggccgc ggcgatcgtc gccggcgcga acgagcgcgg cgagacggtc gcgacgcgca aggcgtcgca gcagacgatc gaggggctcg ccgccgtgct gcccgagctg ctcggcggct cggcggacct gacgggctcg aacctcacga actggaaggc gtcgaaggcg gttcgcgcga atgcggacgg cccgggcgtc cagtggggca accatatcaa ctacggcgtg cgcgaattcg gcatgagcgc cgcgatcaac ggcctcgtgc tgcacggcgg ctacaagccg ttcggcggca cgttcctcac gttctccgac tacagccgca atgcgctgcg cgtcgccgcg ctgatgaagg tgccgtcgat cttcgtgttc acgcacgatt cgatcggcct cggcgaggac ggcccgacgc accagtcggt cgagcatgtc gcgagcctgc gcctgattcc caacctcgac gtctggcgtc cggcggacac ggtggagacg gccgtcgcgt ggacctacgc ggtcgcgcac cagcacccgt cgtgcctgat cttcagccgc cagaacctcg cgttcaacgc gcgcaccgat gcgcagctcg cgaacgtcga gaagggcggc tacgtgctgc gcgactggga cgaagaaatc gtcgcgcgca agatcatcct gatcgcgacg ggctccgaag tcgagctcgc gatgaaggcg gtcgagcccc tcgcgcagca ggggatcgcc gcgcgcgtcg tgtcgatgcc gtcgagcgac gtgttcgacc gccaggacgc cgagtaccgc gagcgggtgc tgccgcacgg cgtgcgccgc gtcgcgatcg aagcgggcgt caccgatttc tggcgcaagt acgtgggcct cgaaggcggc gtggtcggca tcgacacgtt cggcgagtcg gccccggccg gcgtgctgtt caagcatttc ggcttcactg tcgagcacgt gatcgaaacg gcgaaggcgg tgctcgcgta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for ButhA.01294.a.A1.PS01189
PURIFICATION DATe: 8/17/2011
CONCENTRATION: 44.4mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 200µl
PERCENT IDENTITY: 84
PERCENT COVERAGE: 29
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPPVPRFLD SFSGLDMTTS SPASTTLMAN AIRALAMDAV QQANSGHPGM PMGMAXIGVA LWSRHLKHNP TNPHWADRXR FVLSNGHGSM LLYSLLXLXX YDLPIEELXN FRQLHSKTPG HPEYGITXGV XXXTXXLGQG LANAVGMALG EALLAXXFNR DXXXIXXHHT YVXPXXXXPD XXXLARXLLA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgccgc ccgtgccccg ttttctcgac tcgttctccg gactcgacat gacgacttcg tctcccgcct ccaccacctt gatggccaac gcgatccgcg cgctcgcgat ggacgccgtc cagcaagcga actccggtca ccccggcatg ccgatgggca tggccnanat cggcgtcgcg ctgtggtcgc gccacctgaa gcacaacccg acgaacccgc attgggccga ccgnnnccgc ttcgtgctgt cgaacggcca cggctcgatg ctgctgtact cgctgctgcn cctgannngn tacgatctgc cgatcgaaga gctgaanaat ttccggcaac tgcattcgaa gacgccgggc cacccggaat acgggatcac gnnnggcgtn nngannncga cngnnncgct cggccagggc ctcgcgaacg cggtcggcat ggcgctcggc gaggcgctgc tcgccnncna gttcaaccgc gacgannnnn nnatcgncna tcaccacacg tacgtgnttc ctcnnnnncn nntgcctgat gnaangnntc tcgcacgann nctgctcgct c
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPPVPRFLD SFSGLDMTTS SPASTTLMAN AIRALAMDAV QQANSGHPGM PMGMAEIGVA LWSRHLKHNP TNPHWADRDR FVLSNGHGSM LLYSLLHLTG YDLPIEELKN FRQLHSKTPG HPEYGITPGV ETTTGPLGQG LANAVGMALG EALLAAEFNR DDAKIVDHHT YVFLGDGCLM EGISHEACSL AGTLKLNKLI ALYDDNGISI DGDVVNWFHD DTPKRFEAYG WNVIPNVNGH DVDAIDAAIA KAKRSDKPSL ICCKTRIGNG AATKAGGHDV HGAPLGADEI AKTREALGWT WAPFVIPQEV YAAWDAKEAG KRSEDDWNAA FAQYRAKYPA EAAEFERRMA GTLPADWAAK AAAIVAGANE RGETVATRKA SQQTIEGLAA VLPELLGGSA DLTGSNLTNW KASKAVRANA DGPGVQWGNH INYGVREFGM SAAINGLVLH GGYKPFGGTF LTFSDYSRNA LRVAALMKVP SIFVFTHDSI GLGEDGPTHQ SVEHVASLRL IPNLDVWRPA DTVETAVAWT YAVAHQHPSC LIFSRQNLAF NARTDAQLAN VEKGGYVLRD WDEEIVARKI ILIATGSEVE LAMKAVEPLA QQGIAARVVS MPSSDVFDRQ DAEYRERVLP HGVRRVAIEA GVTDFWRKYV GLEGGVVGID TFGESAPAGV LFKHFGFTVE HVIETAKAVL A
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCCGCCCGTG CCCCGTTTTC TCGACTCGTT CTCCGGACTC GACATGACGA CTTCGTCTCC CGCCTCCACC ACCTTGATGG CCAACGCGAT CCGCGCGCTC GCGATGGACG CCGTCCAGCA AGCGAACTCC GGTCACCCCG GCATGCCGAT GGGCATGGCC GAGATCGGCG TCGCGCTGTG GTCGCGCCAC CTGAAGCACA ACCCGACGAA CCCGCATTGG GCCGACCGCG ACCGCTTCGT GCTGTCGAAC GGCCACGGCT CGATGCTGCT GTACTCGCTG CTGCACCTGA CAGGCTACGA TCTGCCGATC GAAGAGCTGA AGAATTTCCG GCAACTGCAT TCGAAGACGC CGGGCCACCC GGAATACGGG ATCACGCCGG GCGTCGAGAC GACGACGGGC CCGCTCGGCC AGGGCCTCGC GAACGCGGTC GGCATGGCGC TCGGCGAGGC GCTGCTCGCC GCCGAGTTCA ACCGCGACGA CGCGAAGATC GTCGATCACC ACACGTACGT GTTCCTCGGC GACGGCTGCC TGATGGAAGG CATCTCGCAC GAGGCCTGCT CGCTCGCGGG CACGCTGAAG CTCAACAAGC TGATCGCGCT CTATGACGAC AACGGGATCT CGATCGACGG CGACGTCGTC AACTGGTTCC ACGACGACAC GCCGAAGCGC TTCGAGGCGT ACGGCTGGAA CGTGATTCCG AACGTGAACG GCCATGACGT CGACGCGATC GACGCGGCGA TCGCGAAGGC GAAGCGCTCG GACAAGCCGA GCCTCATCTG CTGCAAGACG CGCATCGGCA ACGGCGCGGC GACGAAGGCG GGCGGCCACG ACGTGCACGG CGCGCCGCTC GGCGCGGACG AAATCGCGAA GACCCGCGAG GCGCTCGGCT GGACGTGGGC GCCGTTCGTG ATTCCGCAGG AAGTCTATGC GGCATGGGAC GCGAAGGAGG CGGGCAAGCG CAGCGAAGAC GACTGGAACG CGGCGTTCGC GCAATACCGC GCGAAGTACC CGGCCGAAGC GGCCGAATTC GAGCGCCGGA TGGCGGGCAC GCTGCCGGCC GATTGGGCCG CGAAGGCCGC GGCGATCGTC GCCGGCGCGA ACGAGCGCGG CGAGACGGTC GCGACGCGCA AGGCGTCGCA GCAGACGATC GAGGGGCTCG CCGCCGTGCT GCCCGAGCTG CTCGGCGGCT CGGCGGACCT GACGGGCTCG AACCTCACGA ACTGGAAGGC GTCGAAGGCG GTTCGCGCGA ATGCGGACGG CCCGGGCGTC CAGTGGGGCA ACCATATCAA CTACGGCGTG CGCGAATTCG GCATGAGCGC CGCGATCAAC GGCCTCGTGC TGCACGGCGG CTACAAGCCG TTCGGCGGCA CGTTCCTCAC GTTCTCCGAC TACAGCCGCA ATGCGCTGCG CGTCGCCGCG CTGATGAAGG TGCCGTCGAT CTTCGTGTTC ACGCACGATT CGATCGGCCT CGGCGAGGAC GGCCCGACGC ACCAGTCGGT CGAGCATGTC GCGAGCCTGC GCCTGATTCC CAACCTCGAC GTCTGGCGTC CGGCGGACAC GGTGGAGACG GCCGTCGCGT GGACCTACGC GGTCGCGCAC CAGCACCCGT CGTGCCTGAT CTTCAGCCGC CAGAACCTCG CGTTCAACGC GCGCACCGAT GCGCAGCTCG CGAACGTCGA GAAGGGCGGC TACGTGCTGC GCGACTGGGA CGAAGAAATC GTCGCGCGCA AGATCATCCT GATCGCGACG GGCTCCGAAG TCGAGCTCGC GATGAAGGCG GTCGAGCCCC TCGCGCAGCA GGGGATCGCC GCGCGCGTCG TGTCGATGCC GTCGAGCGAC GTGTTCGACC GCCAGGACGC CGAGTACCGC GAGCGGGTGC TGCCGCACGG CGTGCGCCGC GTCGCGATCG AAGCGGGCGT CACCGATTTC TGGCGCAAGT ACGTGGGCCT CGAAGGCGGC GTGGTCGGCA TCGACACGTT CGGCGAGTCG GCCCCGGCCG GCGTGCTGTT CAAGCATTTC GGCTTCACTG TCGAGCACGT GATCGAAACG GCGAAGGCGG TGCTCGCGTA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A