ButhA.01254.a

Phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase (EC 4.1.1.36) (EC 6.3.2.5) (BTH_I0768) (coaBC)

CENTER ID: ButhA.01254.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.01254.a.A1.GE32761 full length 1 403
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.3485
RefSeq: YP_441324.1
UniProt: Q2T0H5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAHAELAGKH LVLGLTGGIA CYKIAELTRL LTKAGATVQI VMTDAATQFI TPVTMQALSG RPVYTSQWDA RMPNNMAHID LSREADAIVI APASTDFLAK LSHGFADDLL STLCIARDCP LLVVPAMNRQ MWQNPATQRN VAQLRRDGVA VLGPDSGAQA CGEIGDGRML EPEAIYEAIV THFQPKVFAH RRVLITAGPT FEPLDPVRGL TNRSSGKMGF ALARAAQQAG ADVHLVAGPV HLPTPWGVYR EDVRTAQQMY DAVMNAVPDA DVFIAVAAVA DWRAAQPAEH KIKKTADHKM PALTFVENPD ILASVAALPD APYCVGFAAE SGDLDVHGEE KRVRKKVPLL IGNLGPLTFG LDDNEVVLFE ACGATRLPRA DKMTLAHALV AEIAKRLPDA SLI
NT Sequence
ttggcacacg cagaactcgc aggaaaacac ctcgtcctcg gcctcacggg cggcatcgcc tgctacaaga tcgccgagct cacgcgcctc ttgacgaagg cgggcgcgac cgtgcagatc gtgatgaccg acgccgcgac gcagttcatc acccccgtca cgatgcaggc gctgtccggc cgccccgtct acacgagcca gtgggacgcg cggatgccga acaacatggc gcacatcgat ctgtcgcgcg aagccgacgc gatcgtgatc gcgcccgcgt cgaccgattt cctcgcgaag ctctcgcacg gcttcgccga cgatctgctg tcgacgctgt gcatcgcgcg cgactgcccg ctcctcgtcg tgccggcgat gaaccgccag atgtggcaga acccggcgac gcagcgcaac gtcgcgcaac tgcgccgcga cggcgtcgcg gtgctcggcc ccgattcggg cgcgcaggcg tgcggcgaga tcggcgacgg ccggatgctc gagccggaag cgatctacga agcgatcgtc acgcatttcc agccgaaggt gttcgcgcat cgccgcgtgc tgatcacggc cggccccacc ttcgagccgc tcgatccggt tcgcggcctg acgaaccgct cgagcggcaa gatgggcttc gcgctcgcgc gcgccgcgca gcaggcgggc gccgacgtgc atctcgtcgc cggccccgtt cacctgccga cgccgtgggg cgtgtaccgc gaggacgtgc ggaccgcgca gcagatgtac gacgcggtga tgaacgcggt gccggacgcg gacgtgttca tcgcggtcgc cgcggtcgcc gactggcgcg ccgcgcagcc cgccgagcac aagatcaaga agacggccga ccacaagatg ccggcgctca cgttcgtcga gaacccggac attctcgcga gcgtcgccgc gctgcccgat gcgccctact gcgtcggctt cgcggcggaa agcggcgatc tcgacgtgca cggcgaggaa aagcgcgtgc gcaagaaggt gccgctgctg atcggcaacc tcgggccgct cacgttcggg ctcgacgaca acgaagtcgt gctgttcgaa gcgtgcggcg cgacgcggct gccgcgcgcc gacaagatga cgctcgcgca cgcgctcgtc gccgagatcg caaagcgcct gcccgacgcg agcctcatct ga
Details for ButhA.01254.a.A1.GE32761
HARVESTED ON: 6/20/2011
SEQUENCED ON: 3/3/2015
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 92
PERCENT COVERAGE: 68
Validated AA Sequence
MGTLEAQTQG PGSMLAHAEL AGKHLVLGLT GGIACYKIAE LTRLLTKAGA TVQIVMTDAA TQFITPVTMQ ALSGRPVYTS QWDARMPNNM AHIDLSREAD AIVIAPASTD FLAKLSHGFA DDLLSTLCIA RDCPLLVVPA MNRQMWQNPA TQRNVAQLRR DGVAVLGPDS GAQACGEIGD GRMLEPEAIY EAIVTHFQPK VFAHRRVLIT AGPTFEPLDP VRGLTNRSSG KMGFALARAA QXXGADVHLV AGPVHLPTPW GVYREDXRTA XXXXXXXXXX VXXXXXXXXX X
Validated NT Sequence
ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgttggca cacgcagaac tcgcaggaaa acacctcgtc ctcggcctca cgggcggcat cgcctgctac aagatcgccg agctcacgcg cctcttgacg aaggcgggcg cgaccgtgca gatcgtgatg accgacgccg cgacgcagtt catcaccccc gtcacgatgc aggcgctgtc cggccgcccc gtctacacga gccagtggga cgcgcggatg ccgaacaaca tggcgcacat cgatctgtcg cgcgaagccg acgcgatcgt gatcgcgccc gcgtcgaccg atttcctcgc gaagctctcg cacggcttcg ccgacgatct gctgtcgacg ctgtgcatcg cgcgcgactg cccgctcctc gtcgtgccgg cgatgaaccg ccagatgtgg cagaacccgg cgacgcagcg caacgtcgcg caactgcgcc gcgacggcgt cgcggtgctc ggccccgatt cgggcgcgca ggcgtgcggc gagatcggcg acggccggat gctcgagccg gaagcgatct acgaagcgat cgtcacgcat ttccagccga aggtgttcgc gcatcgccgc gtgctgatca cggccggccc caccttcgag ccgctcgatc cggttcgcgg cctgacgaac cgctcgagcg gcaagatggg cttcgcgctc gcgcgcgccg cgcagcnnnn gggcgccgac gtgcatctcg tcgccggccc cgttcacctg ccgacgccgt ggggcgtgta ccgcgaggac ntgcggaccg cgnnnnnnnn gtncnnnnnn nnnatnaann nngtgnnnnn nnnngannnn ntcntnnngg ncnccgcg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLAHAELAG KHLVLGLTGG IACYKIAELT RLLTKAGATV QIVMTDAATQ FITPVTMQAL SGRPVYTSQW DARMPNNMAH IDLSREADAI VIAPASTDFL AKLSHGFADD LLSTLCIARD CPLLVVPAMN RQMWQNPATQ RNVAQLRRDG VAVLGPDSGA QACGEIGDGR MLEPEAIYEA IVTHFQPKVF AHRRVLITAG PTFEPLDPVR GLTNRSSGKM GFALARAAQQ AGADVHLVAG PVHLPTPWGV YREDVRTAQQ MYDAVMNAVP DADVFIAVAA VADWRAAQPA EHKIKKTADH KMPALTFVEN PDILASVAAL PDAPYCVGFA AESGDLDVHG EEKRVRKKVP LLIGNLGPLT FGLDDNEVVL FEACGATRLP RADKMTLAHA LVAEIAKRLP DASLI
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttggcacac gcagaactcg caggaaaaca cctcgtcctc ggcctcacgg gcggcatcgc ctgctacaag atcgccgagc tcacgcgcct cttgacgaag gcgggcgcga ccgtgcagat cgtgatgacc gacgccgcga cgcagttcat cacccccgtc acgatgcagg cgctgtccgg ccgccccgtc tacacgagcc agtgggacgc gcggatgccg aacaacatgg cgcacatcga tctgtcgcgc gaagccgacg cgatcgtgat cgcgcccgcg tcgaccgatt tcctcgcgaa gctctcgcac ggcttcgccg acgatctgct gtcgacgctg tgcatcgcgc gcgactgccc gctcctcgtc gtgccggcga tgaaccgcca gatgtggcag aacccggcga cgcagcgcaa cgtcgcgcaa ctgcgccgcg acggcgtcgc ggtgctcggc cccgattcgg gcgcgcaggc gtgcggcgag atcggcgacg gccggatgct cgagccggaa gcgatctacg aagcgatcgt cacgcatttc cagccgaagg tgttcgcgca tcgccgcgtg ctgatcacgg ccggccccac cttcgagccg ctcgatccgg ttcgcggcct gacgaaccgc tcgagcggca agatgggctt cgcgctcgcg cgcgccgcgc agcaggcggg cgccgacgtg catctcgtcg ccggccccgt tcacctgccg acgccgtggg gcgtgtaccg cgaggacgtg cggaccgcgc agcagatgta cgacgcggtg atgaacgcgg tgccggacgc ggacgtgttc atcgcggtcg ccgcggtcgc cgactggcgc gccgcgcagc ccgccgagca caagatcaag aagacggccg accacaagat gccggcgctc acgttcgtcg agaacccgga cattctcgcg agcgtcgccg cgctgcccga tgcgccctac tgcgtcggct tcgcggcgga aagcggcgat ctcgacgtgc acggcgagga aaagcgcgtg cgcaagaagg tgccgctgct gatcggcaac ctcgggccgc tcacgttcgg gctcgacgac aacgaagtcg tgctgttcga agcgtgcggc gcgacgcggc tgccgcgcgc cgacaagatg acgctcgcgc acgcgctcgt cgccgagatc gcaaagcgcc tgcccgacgc gagcctcatc taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa