ButhA.01250.a

Probable inorganic polyphosphate/ATP-NAD kinase (Poly(P)/ATP NAD kinase) (EC 2.7.1.23) (BTH_I1301) (ppnK)

CENTER ID: ButhA.01250.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.01250.a.A1.GE32818 full length 1 299
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.01250.a.A1.PS01289 full length 1 299

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.4067
RefSeq: YP_441848.1
UniProt: Q2SZ01

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKIGHQFHTV ALVGRSNTPG IAEPLASLAA CIAKRGFEVV FEADTAQALG STGYPALTPA EIGARADVAV VLGGDGTMLG IGRQLAPYKT PLIGINHGRL GFITDIPASD MQEVVPMMLA GSYEREERTL LEARIVRNNE PIYHALAFND VVVNRSGFSG MAELRVSVDG RFMYNQRSDG LIVATPTGST AYALSSQGPI LHPQLQGIVL VPIAPHALSN RPIVLPDDSK IAIQIIGGRD VNVNFDMQSF TALELNDTIE VRRSKHTVPF LHPVGYSYYA TLRKKLHWNE HPSNEEDDE
NT Sequence
atgaaaatcg gccaccaatt ccataccgtc gcgctcgtcg ggcgcagcaa cacgcccggc atcgccgagc cgctcgcgtc gctcgccgcg tgcatcgcga agcgcggctt cgaagtcgtg ttcgaggcgg acaccgcgca ggcgctcggc agcaccggct acccggcgct cacgcccgcc gagatcggcg cgcgcgccga cgtcgcggtc gtgctgggcg gcgacggcac gatgctcggc atcggccgcc agctcgcgcc gtacaagacg ccgctcatcg gcatcaacca cggccggctc ggcttcatca ccgacatccc ggcgtccgac atgcaggaag tcgtgccgat gatgctcgcg ggcagctacg agcgcgagga gcgcacgctg ctcgaggcgc ggatcgtgcg caacaacgag ccgatctacc acgcgctcgc gttcaacgac gtcgtcgtga accgcagcgg cttctccggg atggccgagc tgcgcgtgtc ggtcgacggc cgattcatgt acaaccagcg ctcggacggc ctcatcgtcg cgacgccgac gggctcgacc gcatacgcgc tgtcgtcgca ggggccgatc ctgcatccgc agctgcaggg catcgtgctc gtgccgatcg cgccgcacgc gctgtcgaac cggccgatcg tgctgccgga cgattcgaag atcgcgatcc agatcatcgg cgggcgcgac gtgaacgtga acttcgacat gcagtcgttc accgcgctcg agttgaacga cacgatcgaa gtgcgccgct cgaagcacac ggtgccgttc ctgcacccgg tcggctacag ctattacgcg acgctgcgca agaaactgca ctggaacgaa cacccgtcga acgaagaaga cgacgaataa
Details for ButhA.01250.a.A1.GE32818
HARVESTED ON: 6/28/2011
SEQUENCED ON: 7/22/2011
EXPECTED MW: 34kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMKIGHQF HTVALVGRSN TPGIAEPLAS LAACIAKRGF EVVFEADTAQ ALGSTGYPAL TPAEIGARAD VAVVLGGDGT MLGIGRQLAP YKTPLIGINH GRLGFITDIP ASDMQEVVPM MLAGSYEREE RTLLEARIVR NNEPIYHALA FNDVVVNRSG FSGMAELRVS VDGRFMYNQR SDGLIVATPT GSTAYALSSQ GPILHPQLQG IVLVPIAPHA LSNRPIVLPD DSKIAIQIIG GRDVNVNFDM QSFTALELND TIEVRRSKHT VPFLHPVGYS YYATLRKKLH WNEHPSNEED DE
Validated NT Sequence
gtgctgttta ttattcgtcg tcttcttcgt tcgacgggtg ttcgttccag tgcagtttct tgcgcagcgt cgcgtaatag ctgtagccga ccgggtgcag gaacggcacc gtgtgcttcg agcggcgcac ttcgatcgtg tcgttcaact cgagcgcggt gaacgactgc atgtcgaagt tcacgttcac gtcgcgcccg ccgatgatct ggatcgcgat cttcgaatcg tccggcagca cgatcggccg gttcgacagc gcgtgcggcg cgatcggcac gagcacgatg ccctgcagct gcggatgcag gatcggcccc tgcgacgaca gcgcgtatgc ggtcgagccc gtcggcgtcg cgacgatgag gccgtccgag cgctggttgt acatgaatcg gccgtcgacc gacacgcgca gctcggccat cccggagaag ccgctgcggt tcacgacgac gtcgttgaac gcgagcgcgt ggtagatcgg ctcgttgttg cgcacgatcc gcgcctcgag cagcgtgcgc tcctcgcgct cgtagctgcc cgcgagcatc atcggcacga cttcctgcat gtcggacgcc gggatgtcgg tgatgaagcc gagccggccg tggttgatgc cgatgagcgg cgtcttgtac ggcgcgagct ggcggccgat gccgagcatc gtgccgtcgc cgcccagcac gaccgcgacg tcggcgcgcg cgccgatctc ggcgggcgtg agcgccgggt agccggtgct gccgagcgcc tgcgcggtgt ccgcctcgaa cacgacttcg aagccgcgct tcgcgatgca cgcggcgagc gacgcgagcg gctcggcgat gccgggcgtg ttgctgcgcc cgacgagcgc gacggtatgg aattggtggc cgattttcat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKIGHQFHT VALVGRSNTP GIAEPLASLA ACIAKRGFEV VFEADTAQAL GSTGYPALTP AEIGARADVA VVLGGDGTML GIGRQLAPYK TPLIGINHGR LGFITDIPAS DMQEVVPMML AGSYEREERT LLEARIVRNN EPIYHALAFN DVVVNRSGFS GMAELRVSVD GRFMYNQRSD GLIVATPTGS TAYALSSQGP ILHPQLQGIV LVPIAPHALS NRPIVLPDDS KIAIQIIGGR DVNVNFDMQS FTALELNDTI EVRRSKHTVP FLHPVGYSYY ATLRKKLHWN EHPSNEEDDE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaaatcggc caccaattcc ataccgtcgc gctcgtcggg cgcagcaaca cgcccggcat cgccgagccg ctcgcgtcgc tcgccgcgtg catcgcgaag cgcggcttcg aagtcgtgtt cgaggcggac accgcgcagg cgctcggcag caccggctac ccggcgctca cgcccgccga gatcggcgcg cgcgccgacg tcgcggtcgt gctgggcggc gacggcacga tgctcggcat cggccgccag ctcgcgccgt acaagacgcc gctcatcggc atcaaccacg gccggctcgg cttcatcacc gacatcccgg cgtccgacat gcaggaagtc gtgccgatga tgctcgcggg cagctacgag cgcgaggagc gcacgctgct cgaggcgcgg atcgtgcgca acaacgagcc gatctaccac gcgctcgcgt tcaacgacgt cgtcgtgaac cgcagcggct tctccgggat ggccgagctg cgcgtgtcgg tcgacggccg attcatgtac aaccagcgct cggacggcct catcgtcgcg acgccgacgg gctcgaccgc atacgcgctg tcgtcgcagg ggccgatcct gcatccgcag ctgcagggca tcgtgctcgt gccgatcgcg ccgcacgcgc tgtcgaaccg gccgatcgtg ctgccggacg attcgaagat cgcgatccag atcatcggcg ggcgcgacgt gaacgtgaac ttcgacatgc agtcgttcac cgcgctcgag ttgaacgaca cgatcgaagt gcgccgctcg aagcacacgg tgccgttcct gcacccggtc ggctacagct attacgcgac gctgcgcaag aaactgcact ggaacgaaca cccgtcgaac gaagaagacg acgaataaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for ButhA.01250.a.A1.PS01289
PURIFICATION DATe: 12/1/2011
CONCENTRATION: 13.99mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMKIGHQF HTVALVGRSN TPGIAEPLAS LAACIAKRGF EVVFEADTAQ ALGSTGYPAL TPAEIGARAD VAVVLGGDGT MLGIGRQLAP YKTPLIGINH GRLGFITDIP ASDMQEVVPM MLAGSYEREE RTLLEARIVR NNEPIYHALA FNDVVVNRSG FSGMAELRVS VDGRFMYNQR SDGLIVATPT GSTAYALSSQ GPILHPQLQG IVLVPIAPHA LSNRPIVLPD DSKIAIQIIG GRDVNVNFDM QSFTALELND TIEVRRSKHT VPFLHPVGYS YYATLRKKLH WNEHPSNEED DE
Validated NT Sequence
gtgctgttta ttattcgtcg tcttcttcgt tcgacgggtg ttcgttccag tgcagtttct tgcgcagcgt cgcgtaatag ctgtagccga ccgggtgcag gaacggcacc gtgtgcttcg agcggcgcac ttcgatcgtg tcgttcaact cgagcgcggt gaacgactgc atgtcgaagt tcacgttcac gtcgcgcccg ccgatgatct ggatcgcgat cttcgaatcg tccggcagca cgatcggccg gttcgacagc gcgtgcggcg cgatcggcac gagcacgatg ccctgcagct gcggatgcag gatcggcccc tgcgacgaca gcgcgtatgc ggtcgagccc gtcggcgtcg cgacgatgag gccgtccgag cgctggttgt acatgaatcg gccgtcgacc gacacgcgca gctcggccat cccggagaag ccgctgcggt tcacgacgac gtcgttgaac gcgagcgcgt ggtagatcgg ctcgttgttg cgcacgatcc gcgcctcgag cagcgtgcgc tcctcgcgct cgtagctgcc cgcgagcatc atcggcacga cttcctgcat gtcggacgcc gggatgtcgg tgatgaagcc gagccggccg tggttgatgc cgatgagcgg cgtcttgtac ggcgcgagct ggcggccgat gccgagcatc gtgccgtcgc cgcccagcac gaccgcgacg tcggcgcgcg cgccgatctc ggcgggcgtg agcgccgggt agccggtgct gccgagcgcc tgcgcggtgt ccgcctcgaa cacgacttcg aagccgcgct tcgcgatgca cgcggcgagc gacgcgagcg gctcggcgat gccgggcgtg ttgctgcgcc cgacgagcgc gacggtatgg aattggtggc cgattttcat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKIGHQFHT VALVGRSNTP GIAEPLASLA ACIAKRGFEV VFEADTAQAL GSTGYPALTP AEIGARADVA VVLGGDGTML GIGRQLAPYK TPLIGINHGR LGFITDIPAS DMQEVVPMML AGSYEREERT LLEARIVRNN EPIYHALAFN DVVVNRSGFS GMAELRVSVD GRFMYNQRSD GLIVATPTGS TAYALSSQGP ILHPQLQGIV LVPIAPHALS NRPIVLPDDS KIAIQIIGGR DVNVNFDMQS FTALELNDTI EVRRSKHTVP FLHPVGYSYY ATLRKKLHWN EHPSNEEDDE
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAAAATCGGC CACCAATTCC ATACCGTCGC GCTCGTCGGG CGCAGCAACA CGCCCGGCAT CGCCGAGCCG CTCGCGTCGC TCGCCGCGTG CATCGCGAAG CGCGGCTTCG AAGTCGTGTT CGAGGCGGAC ACCGCGCAGG CGCTCGGCAG CACCGGCTAC CCGGCGCTCA CGCCCGCCGA GATCGGCGCG CGCGCCGACG TCGCGGTCGT GCTGGGCGGC GACGGCACGA TGCTCGGCAT CGGCCGCCAG CTCGCGCCGT ACAAGACGCC GCTCATCGGC ATCAACCACG GCCGGCTCGG CTTCATCACC GACATCCCGG CGTCCGACAT GCAGGAAGTC GTGCCGATGA TGCTCGCGGG CAGCTACGAG CGCGAGGAGC GCACGCTGCT CGAGGCGCGG ATCGTGCGCA ACAACGAGCC GATCTACCAC GCGCTCGCGT TCAACGACGT CGTCGTGAAC CGCAGCGGCT TCTCCGGGAT GGCCGAGCTG CGCGTGTCGG TCGACGGCCG ATTCATGTAC AACCAGCGCT CGGACGGCCT CATCGTCGCG ACGCCGACGG GCTCGACCGC ATACGCGCTG TCGTCGCAGG GGCCGATCCT GCATCCGCAG CTGCAGGGCA TCGTGCTCGT GCCGATCGCG CCGCACGCGC TGTCGAACCG GCCGATCGTG CTGCCGGACG ATTCGAAGAT CGCGATCCAG ATCATCGGCG GGCGCGACGT GAACGTGAAC TTCGACATGC AGTCGTTCAC CGCGCTCGAG TTGAACGACA CGATCGAAGT GCGCCGCTCG AAGCACACGG TGCCGTTCCT GCACCCGGTC GGCTACAGCT ATTACGCGAC GCTGCGCAAG AAACTGCACT GGAACGAACA CCCGTCGAAC GAAGAAGACG ACGAATAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA