ButhA.01240.a

Thiamine-phosphate pyrophosphorylase (EC 2.5.1.3) (BTH_I3005)

CENTER ID: ButhA.01240.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.01240.a.A1.GE32817 full length 1 364
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.5893
RefSeq: YP_443509.1
UniProt: Q2SU90

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSAALPDAFW PPADELSEAA ERIRAALGVW PRPDARTRIC LAPPEHPRAG DLWVAAAGDA DVHIARLTAA GAQAIVIDDV SATLHTGAAR HALASRAPLA DDWIAALAAF LDCGFAAPDA LVLALAWRDG DEARGDDPWP VDPARFPRVL GVPAAPEPAF PPCPQRLGLY PVLPSAEWVE RVLDCGVRTV QLRVKDASPD ALRAEVERAV AAGRRHPDAR VFINDHWRLA LDAGAYGIHL GQEDLETAEL RAIAQAGVRL GLSSHGYYEM LVALQLKPSY LALGPVFATA TKAVAAPPQG LARLARYARF AGPQAPLVAI GGITVDTLGA VLAAGVGSAA VVSAITAAAD YREAIVAMQK NFGR
NT Sequence
atgagcgccg cgctgcccga cgcgttctgg ccgcccgcgg acgaactctc cgaggccgcc gagcggatcc gcgcggcgct cggcgtgtgg ccgcggcccg atgcgcgcac gcgaatctgc ctcgcgccgc ccgagcatcc gcgcgcgggc gacctgtggg tcgccgccgc gggcgacgcc gacgtgcaca tcgcgcggct gaccgcggcg ggcgcgcagg cgatcgtcat cgatgacgta tcggcgacgc tccatacggg cgcggcgcgc cacgcgctcg cttcgcgcgc gccgctcgcc gacgactgga tcgcggcgct cgccgccttt ctcgattgcg gcttcgccgc gcccgacgcg ctcgtgctcg cgctcgcgtg gcgggacggt gacgaggcgc gcggcgacga tccgtggccc gtcgatccgg cgcgctttcc gcgcgtgctc ggcgtgccgg ccgcgcccga gcccgcgttc ccgccgtgtc cgcagcggct cggcctgtat ccggtgctgc cgagcgccga atgggtcgag cgcgtgctcg actgcggcgt gcggaccgtg cagctgcgcg tgaaggacgc gtcgcccgac gcgctgcgcg cggaggtcga gcgggccgtc gccgcgggcc gccgtcatcc ggacgcgcgc gtgttcatca acgatcactg gcggctcgcg ctcgacgcgg gcgcgtacgg catccacctc ggccaggagg atctcgagac cgccgagctt cgagcgatcg cgcaggccgg cgtgcggctc ggcctgtcga gccacggcta ttacgaaatg ctcgtcgcgt tgcagttgaa gccgagctat ctcgcgctcg gcccggtgtt cgcgaccgcg acgaaggcgg tcgccgcgcc gccgcagggc ctcgcgcggc tcgcgcgcta tgcgcgcttc gcggggccgc aggcgccgct cgtcgcgatc ggcggaatca ctgtcgacac actcggcgcg gtgctggcgg cgggcgtcgg cagcgcggcc gtcgtcagcg cgatcacggc agcggccgat taccgggaag cgattgttgc aatgcagaaa aacttcggac gataa
Details for ButhA.01240.a.A1.GE32817
HARVESTED ON: 6/28/2011
SEQUENCED ON: 7/22/2011
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 80
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSAALPDAF WPPADELSEA AERIRAALGV WPRPDARTRI CLAPPEHPRA GDLWVAAAGD ADVHIARLTA AGAQAIVIDD VSATLHTGAA RHALASRAPL ADDWIAALAA FLDCGFAAPD ALVLALAWRD GDEARGDDPW PVDPARFPRV LGVPAAPEPA FPPCPQRLGL YPVLPSAEWV ERVLDCGVRT VQLRVKDASP DALRAXVERA VAAGRRHPDA RVFINDHWRL ALDAGAYGIH LGQEDLETAX LXAIAQAGVR LGLSSHGYYX MLVALQLKPX YLALXPXXAT ATXXXXXAXX XAXYAXXXXX XXRXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXKX XXXXXXXXXX XXXXXX
Validated NT Sequence
attttgttta nctttaagaa ggagatatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatgagc gccgcgctgc ccgacgcgtt ctggccgccc gcggacgaac tctccgaggc cgccgagcgg atccgcgcgg cgctcggcgt gtggccgcgg cccgatgcgc gcacgcgaat ctgcctcgcg ccgcccgagc atccgcgcgc gggcgacctg tgggtcgccg ccgcgggcga cgccgacgtg cacatcgcgc ggctgaccgc ggcgggcgcg caggcgatcg tcatcgatga cgtatcggcg acgctccata cgggcgcggc gcgccacgcg ctcgcttcgc gcgcgccgct cgccgacgac tggatcgcgg cgctcgccgc ctttctcgat tgcggcttcg ccgcgcccga cgcgctcgtg ctcgcgctcg cgtggcggga cggtgacgag gcgcgcggcg acgatccgtg gcccgtcgat ccggcgcgct ttccgcgcgt gctcggcgtg ccggccgcgc ccgagcccgc gttcccgccg tgtccgcagc ggctcggcct gtatccggtg ctgccgagcg ccgaatgggt cgagcgcgtg ctcgactgcg gcgtgcggac cgtgcagctg cgcgtgaagg acgcgtcgcc cgacgcgctg cgcgcggang tcgagcgggc cgtcgccgcg ggccgccgtc atccggacgc gcgcgtgttc atcaacgatc actggcggct cgcgctcgac gcgggcgcgt acggcatcca cctcggccag gaggatctcg agaccgccga ncttcnagcg atcgcgcagg ccggcgtgcg gctcggcctg tcgagccacg gctattacna aatgctcgtc gcgttgcagt tgaagccnan ctatctcgcg ctcngcccnn ngntcgcgac cgcgacgaan nnncncnccn gggcncncgn gntcgcgngc tatgcnnnnt nnnggnnncn gnncnntcgn nnnnntngnn ancnnnnncn annnnnnnnn cnnnnnnctg nngnngnnnn nngnnnnnnn ncnncngnnn nnnnnnnnnn nnnnnnnncn gnanntnnnn annnnaaann nnnnnnnnnn nnnnnnnnnn nnannnnnnn nnnnnnnnnn ncnnnnnnnn na
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSAALPDAF WPPADELSEA AERIRAALGV WPRPDARTRI CLAPPEHPRA GDLWVAAAGD ADVHIARLTA AGAQAIVIDD VSATLHTGAA RHALASRAPL ADDWIAALAA FLDCGFAAPD ALVLALAWRD GDEARGDDPW PVDPARFPRV LGVPAAPEPA FPPCPQRLGL YPVLPSAEWV ERVLDCGVRT VQLRVKDASP DALRAEVERA VAAGRRHPDA RVFINDHWRL ALDAGAYGIH LGQEDLETAE LRAIAQAGVR LGLSSHGYYE MLVALQLKPS YLALGPVFAT ATKAVAAPPQ GLARLARYAR FAGPQAPLVA IGGITVDTLG AVLAAGVGSA AVVSAITAAA DYREAIVAMQ KNFGR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcgccgcg ctgcccgacg cgttctggcc gcccgcggac gaactctccg aggccgccga gcggatccgc gcggcgctcg gcgtgtggcc gcggcccgat gcgcgcacgc gaatctgcct cgcgccgccc gagcatccgc gcgcgggcga cctgtgggtc gccgccgcgg gcgacgccga cgtgcacatc gcgcggctga ccgcggcggg cgcgcaggcg atcgtcatcg atgacgtatc ggcgacgctc catacgggcg cggcgcgcca cgcgctcgct tcgcgcgcgc cgctcgccga cgactggatc gcggcgctcg ccgcctttct cgattgcggc ttcgccgcgc ccgacgcgct cgtgctcgcg ctcgcgtggc gggacggtga cgaggcgcgc ggcgacgatc cgtggcccgt cgatccggcg cgctttccgc gcgtgctcgg cgtgccggcc gcgcccgagc ccgcgttccc gccgtgtccg cagcggctcg gcctgtatcc ggtgctgccg agcgccgaat gggtcgagcg cgtgctcgac tgcggcgtgc ggaccgtgca gctgcgcgtg aaggacgcgt cgcccgacgc gctgcgcgcg gaggtcgagc gggccgtcgc cgcgggccgc cgtcatccgg acgcgcgcgt gttcatcaac gatcactggc ggctcgcgct cgacgcgggc gcgtacggca tccacctcgg ccaggaggat ctcgagaccg ccgagcttcg agcgatcgcg caggccggcg tgcggctcgg cctgtcgagc cacggctatt acgaaatgct cgtcgcgttg cagttgaagc cgagctatct cgcgctcggc ccggtgttcg cgaccgcgac gaaggcggtc gccgcgccgc cgcagggcct cgcgcggctc gcgcgctatg cgcgcttcgc ggggccgcag gcgccgctcg tcgcgatcgg cggaatcact gtcgacacac tcggcgcggt gctggcggcg ggcgtcggca gcgcggccgt cgtcagcgcg atcacggcag cggccgatta ccgggaagcg attgttgcaa tgcagaaaaa cttcggacga taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa