ButhA.01238.a

Seryl-tRNA synthetase (EC 6.1.1.11) (Serine--tRNA ligase) (SerRS) (Seryl-tRNA(Ser/Sec) synthetase) (BTH_I1565) (serS)

CENTER ID: ButhA.01238.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.01238.a.A1.GE32760 full length 1 433
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.01238.a.A1.PS01188 full length 1 433

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.4351
RefSeq: YP_442109.1
UniProt: Q2SY90

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLDIQLLRKD LDGVAKRLAD RGYTLDVAAF SALEAERRAI QTRTEELQAR RNSLSKQIGA MKGRGEDTSA VMAEVGGIGD EMKASAVKLD EIQARLSELM LEMPNVPHES VPVGRDETEN VEVRRWGAPR QFDFDVKDHV DVGTPLGLDF ETGAKLSGAR FTVLRGPIAR LHRALAQFML DTHTQQHGYS ETYTPYIVNP DVLYGTGQLP KFAEDMFRVE KGGAENTVTQ YLISTSEISL TNTVRDSIVD ASALPIKLTA HSPCFRSEAG SYGRDTRGMI RQHQFDKVEM VQIVAPEASY AALDEMVGHA EAILQTLELP YRVVALCTGD MGFSAAKTFD LEVWLPAQNT YREISSCSNT ESFQARRMQA RFRNAQGKPE LVHTLNGSGL AVGRTLVAVL ENYQNADGSV TVPVALRPYM GGVERIAAPS SAA
NT Sequence
atgctcgaca tccagttgct gcgcaaagac ctcgacggcg tcgcgaaacg cctcgccgat cgcggctaca ccctcgacgt cgccgcgttt tccgcgctcg aagcggaacg ccgggcgatc cagacacgca cggaagaact gcaggcgcgc cgcaacagcc tgtcgaagca gatcggcgcg atgaaggggc ggggcgagga cacgtcggcg gtgatggccg aggtgggcgg catcggcgac gagatgaagg cctcggcggt gaagctcgac gagattcagg cgcgcctgtc cgagctgatg ctggagatgc cgaacgtgcc gcacgagagc gtgcccgtcg gccgcgacga gacggagaac gtcgaagtgc gccgctgggg cgcgccgcgc cagttcgatt tcgacgtgaa ggaccacgtc gatgtgggca cgccgctcgg cctcgatttc gagacgggcg cgaagctctc cggcgcgcgc ttcacggtgc tgcgcgggcc gatcgcgcgc ctgcatcgcg cgctcgcgca gttcatgctg gacacgcaca cgcagcagca cggctacagc gagacgtaca cgccttacat cgtgaatccg gacgtgctgt acggcacggg ccagttgccg aagttcgcgg aggacatgtt ccgcgtcgag aagggcggcg cggagaacac ggtcacgcaa tacctgatct cgacctcgga gatctcgctg acgaacaccg tgcgcgattc gatcgtcgac gcgtccgcgc tgccgatcaa gctgaccgcg cattcgccgt gcttccgctc ggaggcgggc tcgtacggcc gcgacacgcg cgggatgatc cgccagcacc agttcgacaa ggtcgagatg gtgcagatcg tcgcgccgga agcatcgtac gcggcgctcg acgagatggt cggccacgcg gaggcgatcc tgcagacgct cgaactgccg taccgcgtgg tcgcgctatg cacgggcgac atgggtttct cggcggcgaa gacgttcgac ctcgaggtgt ggctgcccgc gcagaacacg tatcgcgaga tctcgagctg ctcgaatacc gaatcgttcc aggcgcgccg catgcaggcg cggttccgca acgcgcaggg caagcccgag ctcgtgcata cgctgaacgg ctcgggcctc gcggtcggcc gcacgctcgt cgcggtgctg gagaactacc agaacgccga cggctcggtg acggtgcccg tcgcgttgcg gccttacatg ggcggcgtcg agcggatcgc cgcgccttcg agcgcggcct ga
Details for ButhA.01238.a.A1.GE32760
HARVESTED ON: 6/20/2011
SEQUENCED ON: 7/14/2011
EXPECTED MW: 49kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLDIQLLRK DLDGVAKRLA DRGYTLDVAA FSALEAERRA IQTRTEELQA RRNSLSKQIG AMKGRGEDTS AVMAEVGGIG DEMKASAVKL DEIQARLSEL MLEMPNVPHE SVPVGRDETE NVEVRRWGAP RQFDFDVKDH VDVGTPLGLD FETGAKLSGA RFTVLRGPIA RLHRALAQFM LDTHTQQHGY SETYTPYIVN PDVLYGTGQL PKFAEDMFRV EKGGAENTVT QYLISTSEIS LTNTVRDSIV DASALPIKLT AHSPCFRSEA GSYGRDTRGM IRQHQFDKVE MVQIVAPEAS YAALDEMVGH AEAILQTLEL PYRVVALCTG DMGFSAAKTF DLEVWLPAQN TYREISSCSN TESFQARRMQ ARFRNAQGKP ELVHTLNGSG LAVGRTLVAV LENYQNADGS VTVPVALRPY MGGVERIAAP SS
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgctcg acatccagtt gctgcgcaaa gacctcgacg gcgtcgcgaa acgcctcgcc gatcgcggct acaccctcga cgtcgccgcg ttttccgcgc tcgaagcgga acgccgggcg atccagacac gcacggaaga actgcaggcg cgccgcaaca gcctgtcgaa gcagatcggc gcgatgaagg ggcggggcga ggacacgtcg gcggtgatgg ccgaggtggg cggcatcggc gacgagatga aggcctcggc ggtgaagctc gacgagattc aggcgcgcct gtccgagctg atgctggaga tgccgaacgt gccgcacgag agcgtgcccg tcggccgcga cgagacggag aacgtcgaag tgcgccgctg gggcgcgccg cgccagttcg atttcgacgt gaaggaccac gtcgatgtgg gcacgccgct cggcctcgat ttcgagacgg gcgcgaagct ctccggcgcg cgcttcacgg tgctgcgcgg gccgatcgcg cgcctgcatc gcgcgctcgc gcagttcatg ctggacacgc acacgcagca gcacggctac agcgagacgt acacgcctta catcgtgaat ccggacgtgc tgtacggcac gggccagttg ccgaagttcg cggaggacat gttccgcgtc gagaagggcg gcgcggagaa cacggtcacg caatacctga tctcgacctc ggagatctcg ctgacgaaca ccgtgcgcga ttcgatcgtc gacgcgtccg cgctgccgat caagctgacc gcgcattcgc cgtgcttccg ctcggaggcg ggctcgtacg gccgcgacac gcgcgggatg atccgccagc accagttcga caaggtcgag atggtgcaga tcgtcgcgcc ggaagcatcg tacgcggcgc tcgacgagat ggtcggccac gcggaggcga tcctgcagac gctcgaactg ccgtaccgcg tggtcgcgct atgcacgggc gacatgggtt tctcggcggc gaagacgttc gacctcgagg tgtggctgcc cgcgcagaac acgtatcgcg agatctcgag ctgctcgaat accgaatcgt tccaggcgcg ccgcatgcag gcgcggttcc gcaacgcgca gggcaagccc gagctcgtgc atacgctgaa cggctcgggc ctcgcggtcg gccgcacgct cgtcgcggtg ctggagaact accagaacgc cgacggctcg gtgacggtgc ccgtcgcgtt gcggccttac atgggcggcg tcgagcggat cgccgcgcct tcgagcg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLDIQLLRK DLDGVAKRLA DRGYTLDVAA FSALEAERRA IQTRTEELQA RRNSLSKQIG AMKGRGEDTS AVMAEVGGIG DEMKASAVKL DEIQARLSEL MLEMPNVPHE SVPVGRDETE NVEVRRWGAP RQFDFDVKDH VDVGTPLGLD FETGAKLSGA RFTVLRGPIA RLHRALAQFM LDTHTQQHGY SETYTPYIVN PDVLYGTGQL PKFAEDMFRV EKGGAENTVT QYLISTSEIS LTNTVRDSIV DASALPIKLT AHSPCFRSEA GSYGRDTRGM IRQHQFDKVE MVQIVAPEAS YAALDEMVGH AEAILQTLEL PYRVVALCTG DMGFSAAKTF DLEVWLPAQN TYREISSCSN TESFQARRMQ ARFRNAQGKP ELVHTLNGSG LAVGRTLVAV LENYQNADGS VTVPVALRPY MGGVERIAAP SSAA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gctcgacatc cagttgctgc gcaaagacct cgacggcgtc gcgaaacgcc tcgccgatcg cggctacacc ctcgacgtcg ccgcgttttc cgcgctcgaa gcggaacgcc gggcgatcca gacacgcacg gaagaactgc aggcgcgccg caacagcctg tcgaagcaga tcggcgcgat gaaggggcgg ggcgaggaca cgtcggcggt gatggccgag gtgggcggca tcggcgacga gatgaaggcc tcggcggtga agctcgacga gattcaggcg cgcctgtccg agctgatgct ggagatgccg aacgtgccgc acgagagcgt gcccgtcggc cgcgacgaga cggagaacgt cgaagtgcgc cgctggggcg cgccgcgcca gttcgatttc gacgtgaagg accacgtcga tgtgggcacg ccgctcggcc tcgatttcga gacgggcgcg aagctctccg gcgcgcgctt cacggtgctg cgcgggccga tcgcgcgcct gcatcgcgcg ctcgcgcagt tcatgctgga cacgcacacg cagcagcacg gctacagcga gacgtacacg ccttacatcg tgaatccgga cgtgctgtac ggcacgggcc agttgccgaa gttcgcggag gacatgttcc gcgtcgagaa gggcggcgcg gagaacacgg tcacgcaata cctgatctcg acctcggaga tctcgctgac gaacaccgtg cgcgattcga tcgtcgacgc gtccgcgctg ccgatcaagc tgaccgcgca ttcgccgtgc ttccgctcgg aggcgggctc gtacggccgc gacacgcgcg ggatgatccg ccagcaccag ttcgacaagg tcgagatggt gcagatcgtc gcgccggaag catcgtacgc ggcgctcgac gagatggtcg gccacgcgga ggcgatcctg cagacgctcg aactgccgta ccgcgtggtc gcgctatgca cgggcgacat gggtttctcg gcggcgaaga cgttcgacct cgaggtgtgg ctgcccgcgc agaacacgta tcgcgagatc tcgagctgct cgaataccga atcgttccag gcgcgccgca tgcaggcgcg gttccgcaac gcgcagggca agcccgagct cgtgcatacg ctgaacggct cgggcctcgc ggtcggccgc acgctcgtcg cggtgctgga gaactaccag aacgccgacg gctcggtgac ggtgcccgtc gcgttgcggc cttacatggg cggcgtcgag cggatcgccg cgccttcgag cgcggcctaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for ButhA.01238.a.A1.PS01188
PURIFICATION DATe: 8/16/2011
CONCENTRATION: 72.3mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLDIQLLRK DLDGVAKRLA DRGYTLDVAA FSALEAERRA IQTRTEELQA RRNSLSKQIG AMKGRGEDTS AVMAEVGGIG DEMKASAVKL DEIQARLSEL MLEMPNVPHE SVPVGRDETE NVEVRRWGAP RQFDFDVKDH VDVGTPLGLD FETGAKLSGA RFTVLRGPIA RLHRALAQFM LDTHTQQHGY SETYTPYIVN PDVLYGTGQL PKFAEDMFRV EKGGAENTVT QYLISTSEIS LTNTVRDSIV DASALPIKLT AHSPCFRSEA GSYGRDTRGM IRQHQFDKVE MVQIVAPEAS YAALDEMVGH AEAILQTLEL PYRVVALCTG DMGFSAAKTF DLEVWLPAQN TYREISSCSN TESFQARRMQ ARFRNAQGKP ELVHTLNGSG LAVGRTLVAV LENYQNADGS VTVPVALRPY MGGVERIAAP SS
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgctcg acatccagtt gctgcgcaaa gacctcgacg gcgtcgcgaa acgcctcgcc gatcgcggct acaccctcga cgtcgccgcg ttttccgcgc tcgaagcgga acgccgggcg atccagacac gcacggaaga actgcaggcg cgccgcaaca gcctgtcgaa gcagatcggc gcgatgaagg ggcggggcga ggacacgtcg gcggtgatgg ccgaggtggg cggcatcggc gacgagatga aggcctcggc ggtgaagctc gacgagattc aggcgcgcct gtccgagctg atgctggaga tgccgaacgt gccgcacgag agcgtgcccg tcggccgcga cgagacggag aacgtcgaag tgcgccgctg gggcgcgccg cgccagttcg atttcgacgt gaaggaccac gtcgatgtgg gcacgccgct cggcctcgat ttcgagacgg gcgcgaagct ctccggcgcg cgcttcacgg tgctgcgcgg gccgatcgcg cgcctgcatc gcgcgctcgc gcagttcatg ctggacacgc acacgcagca gcacggctac agcgagacgt acacgcctta catcgtgaat ccggacgtgc tgtacggcac gggccagttg ccgaagttcg cggaggacat gttccgcgtc gagaagggcg gcgcggagaa cacggtcacg caatacctga tctcgacctc ggagatctcg ctgacgaaca ccgtgcgcga ttcgatcgtc gacgcgtccg cgctgccgat caagctgacc gcgcattcgc cgtgcttccg ctcggaggcg ggctcgtacg gccgcgacac gcgcgggatg atccgccagc accagttcga caaggtcgag atggtgcaga tcgtcgcgcc ggaagcatcg tacgcggcgc tcgacgagat ggtcggccac gcggaggcga tcctgcagac gctcgaactg ccgtaccgcg tggtcgcgct atgcacgggc gacatgggtt tctcggcggc gaagacgttc gacctcgagg tgtggctgcc cgcgcagaac acgtatcgcg agatctcgag ctgctcgaat accgaatcgt tccaggcgcg ccgcatgcag gcgcggttcc gcaacgcgca gggcaagccc gagctcgtgc atacgctgaa cggctcgggc ctcgcggtcg gccgcacgct cgtcgcggtg ctggagaact accagaacgc cgacggctcg gtgacggtgc ccgtcgcgtt gcggccttac atgggcggcg tcgagcggat cgccgcgcct tcgagcg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLDIQLLRK DLDGVAKRLA DRGYTLDVAA FSALEAERRA IQTRTEELQA RRNSLSKQIG AMKGRGEDTS AVMAEVGGIG DEMKASAVKL DEIQARLSEL MLEMPNVPHE SVPVGRDETE NVEVRRWGAP RQFDFDVKDH VDVGTPLGLD FETGAKLSGA RFTVLRGPIA RLHRALAQFM LDTHTQQHGY SETYTPYIVN PDVLYGTGQL PKFAEDMFRV EKGGAENTVT QYLISTSEIS LTNTVRDSIV DASALPIKLT AHSPCFRSEA GSYGRDTRGM IRQHQFDKVE MVQIVAPEAS YAALDEMVGH AEAILQTLEL PYRVVALCTG DMGFSAAKTF DLEVWLPAQN TYREISSCSN TESFQARRMQ ARFRNAQGKP ELVHTLNGSG LAVGRTLVAV LENYQNADGS VTVPVALRPY MGGVERIAAP SSAA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCTCGACATC CAGTTGCTGC GCAAAGACCT CGACGGCGTC GCGAAACGCC TCGCCGATCG CGGCTACACC CTCGACGTCG CCGCGTTTTC CGCGCTCGAA GCGGAACGCC GGGCGATCCA GACACGCACG GAAGAACTGC AGGCGCGCCG CAACAGCCTG TCGAAGCAGA TCGGCGCGAT GAAGGGGCGG GGCGAGGACA CGTCGGCGGT GATGGCCGAG GTGGGCGGCA TCGGCGACGA GATGAAGGCC TCGGCGGTGA AGCTCGACGA GATTCAGGCG CGCCTGTCCG AGCTGATGCT GGAGATGCCG AACGTGCCGC ACGAGAGCGT GCCCGTCGGC CGCGACGAGA CGGAGAACGT CGAAGTGCGC CGCTGGGGCG CGCCGCGCCA GTTCGATTTC GACGTGAAGG ACCACGTCGA TGTGGGCACG CCGCTCGGCC TCGATTTCGA GACGGGCGCG AAGCTCTCCG GCGCGCGCTT CACGGTGCTG CGCGGGCCGA TCGCGCGCCT GCATCGCGCG CTCGCGCAGT TCATGCTGGA CACGCACACG CAGCAGCACG GCTACAGCGA GACGTACACG CCTTACATCG TGAATCCGGA CGTGCTGTAC GGCACGGGCC AGTTGCCGAA GTTCGCGGAG GACATGTTCC GCGTCGAGAA GGGCGGCGCG GAGAACACGG TCACGCAATA CCTGATCTCG ACCTCGGAGA TCTCGCTGAC GAACACCGTG CGCGATTCGA TCGTCGACGC GTCCGCGCTG CCGATCAAGC TGACCGCGCA TTCGCCGTGC TTCCGCTCGG AGGCGGGCTC GTACGGCCGC GACACGCGCG GGATGATCCG CCAGCACCAG TTCGACAAGG TCGAGATGGT GCAGATCGTC GCGCCGGAAG CATCGTACGC GGCGCTCGAC GAGATGGTCG GCCACGCGGA GGCGATCCTG CAGACGCTCG AACTGCCGTA CCGCGTGGTC GCGCTATGCA CGGGCGACAT GGGTTTCTCG GCGGCGAAGA CGTTCGACCT CGAGGTGTGG CTGCCCGCGC AGAACACGTA TCGCGAGATC TCGAGCTGCT CGAATACCGA ATCGTTCCAG GCGCGCCGCA TGCAGGCGCG GTTCCGCAAC GCGCAGGGCA AGCCCGAGCT CGTGCATACG CTGAACGGCT CGGGCCTCGC GGTCGGCCGC ACGCTCGTCG CGGTGCTGGA GAACTACCAG AACGCCGACG GCTCGGTGAC GGTGCCCGTC GCGTTGCGGC CTTACATGGG CGGCGTCGAG CGGATCGCCG CGCCTTCGAG CGCGGCCTAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA