ButhA.01187.a
Glutamyl-tRNA synthetase (EC 6.1.1.17) (Glutamate--tRNA ligase) (GluRS) (BTH_I1984) (gltX)
| CENTER ID: | ButhA.01187.a |
| ORGANISM: | Burkholderia thailandensis E264 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.01187.a.A1.GE32759 | full length | 1 | 469 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
4G6Z
DEPOSITED: 7/19/2012
DETERMINATION: XRay
CLONE: ButhA.01187.a.A1.GE32759
PROTEIN: ButhA.01187.a.A1.PS01190
Publications by SSGCID
Ligand co-crystallization of aminoacyl-tRNA synthetases from infectious disease organisms
Clifton MC, Dranow DM, Edwards TE, Lorimer DD, Manoil C, Moen S, Myler PJ, Sankaran B, Sharma A, Staker BL, Van Voorhis WC
Sci Rep - 2017
volume 7, issue 1, pages 223
PMID: 28303005; PMCID: PMC5428304
Combining functional and structural genomics to sample the essential Burkholderia structome.
Abendroth J, Armour B, Barrett L, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Fox D, Gallagher LA, Gardberg AS, Gillespie A, Manoil C, Myler PJ, Nakazawa-Hewitt S, Napuli A, Nguyen MT, Patrapuvich R, Phan I, Stacy R, Staker BL, Stewart LJ, Van Voorhis WC
PLoS ONE - 2012
volume 8, issue 1, pages e53851
PMID: 23382856; PMCID: PMC3561365
PDB:
3D63,
3DAH,
3EIZ,
3EJ2,
3EK2,
3EZO,
3F0F,
3FTP,
3GK0,
3GK3,
3GVF,
3GWA,
3GWE,
3IML,
3SZ8,
3T4C,
3TML,
3TMQ,
3TXY,
3U7J,
3UE9,
3UK1,
3UK2,
3UND,
3UPT,
3URR,
3UW1,
3UW2,
3UW3,
3V2I,
3V7N,
3V8H,
3V9O,
3V9P,
3VAV,
4DDO,
4DFE,
4DHE,
4DHK,
4DUT,
4DZ4,
4E4T,
4E51,
4EFI,
4EG0,
4EGJ,
4EK2,
4EQY,
4EWG,
4EX5,
4EXQ,
4F2G,
4F32,
4F3N,
4F3Y,
4F4H,
4F7D,
4FK8,
4FRY,
4G1K,
4G67,
4G6Z,
4GHK,
4H3Y,
4H3Z,
4H4G
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_127621 |
| PATRIC ID: | fig|271848.6.peg.4795 |
| RefSeq: | YP_442513.1 |
| UniProt: | Q2SX36 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTRPVRTRFA PSPTGFIHLG NIRSALYPWA FARKMKGTFV LRIEDTDVER SSQEAVDAIL EGMAWLGLDY DEGPYYQMQR MDRYREVLAQ MQEKGLVYPC YMSTEELDAL RERQRAAGEK PRYDGTWRPE PGKVLPEPPA GVAPVLRFRN PLTGTVAWDD AVKGRVEISN EELDDLVVAR PDGTPMYNFC VVVDDLDMGI THVIRGDDHV NNTPRQINIL RALGGEVPVY AHLPTVLNEQ GEKMSKRHGA MSVMGYRDAG YLPEAVLNYL ARLGWSHGDA EIFTREQFVE WFDLEHLGKS PAQYDHNKLN WLNNHYIKEA DDARLAGLAK PFFAALGIDA GAIEQGPDLV SVMGLMKDRA STVKEIAENS AMFYRAPAPG ADALAQHVTD AVRPALVEFA AALKTVEWTK EAIAAALKAV LGAHKLKMPQ LAMPVRLLVA GTTHTPSIDA VLLLFGRDVV VSRIEAALA
NT Sequence
atgacccgtc ctgtacgcac ccgttttgcg ccgagcccca ccggcttcat ccatctcggc aacatccgat ccgcgcttta tccgtgggcg ttcgcccgca agatgaaggg cacgttcgtg ctgcggatcg aggacaccga tgtcgagcgt tcgagccagg aggcggtcga cgcgatcctc gaagggatgg cgtggctcgg cctcgattac gacgaaggcc cgtactatca gatgcagcgg atggatcgct atcgcgaagt gctcgcgcag atgcaggaga agggcctcgt ctacccgtgc tacatgtcga ccgaagagct cgacgcgttg cgcgagcgcc agcgcgcggc gggcgagaag ccgcgctacg acggcacgtg gcgcccggag ccgggcaagg tgctgcccga gccgcccgcc ggcgtcgcgc ccgtgctgcg cttccgcaat ccgctgacag gcacggtggc ctgggacgat gcggtgaagg gccgcgtcga gatctcgaac gaggaactcg acgatctcgt cgtcgcgcgt cccgacggca cgccgatgta caacttctgc gtcgtcgtcg acgatctcga catgggcatc acgcacgtga tccgcggcga cgaccacgtg aacaacacgc cgcgccagat caacatcctg cgcgcgctcg gcggcgaagt gccggtgtac gcgcacttgc cgaccgtgct caacgagcag ggcgagaaga tgagcaagcg tcacggcgcg atgagcgtga tgggctatcg cgatgccggc tatctgcctg aggcggtgct caactatctc gcgcgccttg gctggtcgca cggcgacgcg gagatcttca cgcgcgagca gttcgtcgaa tggttcgacc tcgagcatct cggcaagtcg cccgcgcagt acgaccataa caagctgaac tggttgaaca accattacat caaggaagcg gacgacgcgc gtctcgccgg gctggcgaag ccgttcttcg cggcgctcgg catcgacgcc ggcgcgatcg agcaggggcc ggacctcgta agcgtgatgg ggctgatgaa ggaccgcgcg tcgacggtga aggaaatcgc ggagaattcg gcgatgttct accgtgcgcc cgcgcccggc gcggatgcgc tcgcgcagca cgtgaccgac gcggtgcgtc cggcgctcgt ggagttcgcg gcggcgctga agacggtcga atggacgaag gaggcgatcg cggccgcgct gaaggccgtg ctcggtgcgc acaagctgaa gatgccgcag ctcgcgatgc cggtgcgcct gctcgtggcg ggcacgacgc acacgccgtc gatcgacgcg gtgctgctgc tgttcggccg cgacgtcgtc gtgtcgcgta tcgaggcggc gctcgcgtaa
Details for ButhA.01187.a.A1.GE32759
| HARVESTED ON: | 6/20/2011 |
| SEQUENCED ON: | 7/14/2011 |
| EXPECTED MW: | 54kDa |
| OBSERVED MW: | 54kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 99 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTRPVRTRF APSPTGFIHL GNIRSALYPW AFARKMKGTF VLRIEDTDVE RSSQEAVDAI LEGMAWLGLD YDEGPYYQMQ RMDRYREVLA QMQEKGLVYP CYMSTEELDA LRERQRAAGE KPRYDGTWRP EPGKVLPEPP AGVAPVLRFR NPLTGTVAWD DAVKGRVEIS NEELDDLVVA RPDGTPMYNF CVVVDDLDMG ITHVIRGDDH VNNTPRQINI LRALGGEVPV YAHLPTVLNE QGEKMSXRHG AMSVMGYRDA GYLPEAVLNY LARLGWSHGD AEIFTREQFV EWFDLEHLGK SPAQYDHNKL NWLNNHYIKE ADDARLAGLA KPFFAALGID AGAIEQGPDL VSVMGLMKDR ASTVKEIAEN SAMFYRAPAP GADALAQHVT DAVRPALVEF AAALKTVEWT KEAIAAALKA VLGAHKLKMP QLAMPVRLLV AGTTHTPSID AVLLLFGRDV VVSRIEAA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaccc gtcctgtacg cacccgtttt gcgccgagcc ccaccggctt catccatctc ggcaacatcc gatccgcgct ttatccgtgg gcgttcgccc gcaagatgaa gggcacgttc gtgctgcgga tcgaggacac cgatgtcgag cgttcgagcc aggaggcggt cgacgcgatc ctcgaaggga tggcgtggct cggcctcgat tacgacgaag gcccgtacta tcagatgcag cggatggatc gctatcgcga agtgctcgcg cagatgcagg agaagggcct cgtctacccg tgctacatgt cgaccgaaga gctcgacgcg ttgcgcgagc gccagcgcgc ggcgggcgag aagccgcgct acgacggcac gtggcgcccg gagccgggca aggtgctgcc cgagccgccc gccggcgtcg cgcccgtgct gcgcttccgc aatccgctga caggcacggt ggcctgggac gatgcggtga agggccgcgt cgagatctcg aacgaggaac tcgacgatct cgtcgtcgcg cgtcccgacg gcacgccgat gtacaacttc tgcgtcgtcg tcgacgatct cgacatgggc atcacgcacg tgatccgcgg cgacgaccac gtgaacaaca cgccgcgcca gatcaacatc ctgcgcgcgc tcggcggcga agtgccggtg tacgcgcact tgccgaccgt gctcaacgag cagggcgaga agatgagcna gcgtcacggc gcgatgagcg tgatgggcta tcgcgatgcc ggctatctgc ctgaggcggt gctcaactat ctcgcgcgcc ttggctggtc gcacggcgac gcggagatct tcacgcgcga gcagttcgtc gaatggttcg acctcgagca tctcggcaag tcgcccgcgc agtacgacca taacaagctg aactggttga acaaccatta catcaaggaa gcggacgacg cgcgtctcgc cgggctggcg aagccgttct tcgcggcgct cggcatcgac gccggcgcga tcgagcaggg gccggacctc gtaagcgtga tggggctgat gaaggaccgc gcgtcgacgg tgaaggaaat cgcggagaat tcggcgatgt tctaccgtgc gcccgcgccc ggcgcggatg cgctcgcgca gcacgtgacc gacgcggtgc gtccggcgct cgtggagttc gcggcggcgc tgaagacggt cgaatggacg aaggaggcga tcgcggccgc gctgaaggcc gtgctcggtg cgcacaagct gaagatgccg cagctcgcga tgccggtgcg cctgctcgtg gcgggcacga cgcacacgcc gtcgatcgac gcggtgctgc tgctgttcgg ccgcgacgtc gtcgtgtcgc gtatcgaggc ggcgc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTRPVRTRF APSPTGFIHL GNIRSALYPW AFARKMKGTF VLRIEDTDVE RSSQEAVDAI LEGMAWLGLD YDEGPYYQMQ RMDRYREVLA QMQEKGLVYP CYMSTEELDA LRERQRAAGE KPRYDGTWRP EPGKVLPEPP AGVAPVLRFR NPLTGTVAWD DAVKGRVEIS NEELDDLVVA RPDGTPMYNF CVVVDDLDMG ITHVIRGDDH VNNTPRQINI LRALGGEVPV YAHLPTVLNE QGEKMSKRHG AMSVMGYRDA GYLPEAVLNY LARLGWSHGD AEIFTREQFV EWFDLEHLGK SPAQYDHNKL NWLNNHYIKE ADDARLAGLA KPFFAALGID AGAIEQGPDL VSVMGLMKDR ASTVKEIAEN SAMFYRAPAP GADALAQHVT DAVRPALVEF AAALKTVEWT KEAIAAALKA VLGAHKLKMP QLAMPVRLLV AGTTHTPSID AVLLLFGRDV VVSRIEAALA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacccgtcct gtacgcaccc gttttgcgcc gagccccacc ggcttcatcc atctcggcaa catccgatcc gcgctttatc cgtgggcgtt cgcccgcaag atgaagggca cgttcgtgct gcggatcgag gacaccgatg tcgagcgttc gagccaggag gcggtcgacg cgatcctcga agggatggcg tggctcggcc tcgattacga cgaaggcccg tactatcaga tgcagcggat ggatcgctat cgcgaagtgc tcgcgcagat gcaggagaag ggcctcgtct acccgtgcta catgtcgacc gaagagctcg acgcgttgcg cgagcgccag cgcgcggcgg gcgagaagcc gcgctacgac ggcacgtggc gcccggagcc gggcaaggtg ctgcccgagc cgcccgccgg cgtcgcgccc gtgctgcgct tccgcaatcc gctgacaggc acggtggcct gggacgatgc ggtgaagggc cgcgtcgaga tctcgaacga ggaactcgac gatctcgtcg tcgcgcgtcc cgacggcacg ccgatgtaca acttctgcgt cgtcgtcgac gatctcgaca tgggcatcac gcacgtgatc cgcggcgacg accacgtgaa caacacgccg cgccagatca acatcctgcg cgcgctcggc ggcgaagtgc cggtgtacgc gcacttgccg accgtgctca acgagcaggg cgagaagatg agcaagcgtc acggcgcgat gagcgtgatg ggctatcgcg atgccggcta tctgcctgag gcggtgctca actatctcgc gcgccttggc tggtcgcacg gcgacgcgga gatcttcacg cgcgagcagt tcgtcgaatg gttcgacctc gagcatctcg gcaagtcgcc cgcgcagtac gaccataaca agctgaactg gttgaacaac cattacatca aggaagcgga cgacgcgcgt ctcgccgggc tggcgaagcc gttcttcgcg gcgctcggca tcgacgccgg cgcgatcgag caggggccgg acctcgtaag cgtgatgggg ctgatgaagg accgcgcgtc gacggtgaag gaaatcgcgg agaattcggc gatgttctac cgtgcgcccg cgcccggcgc ggatgcgctc gcgcagcacg tgaccgacgc ggtgcgtccg gcgctcgtgg agttcgcggc ggcgctgaag acggtcgaat ggacgaagga ggcgatcgcg gccgcgctga aggccgtgct cggtgcgcac aagctgaaga tgccgcagct cgcgatgccg gtgcgcctgc tcgtggcggg cacgacgcac acgccgtcga tcgacgcggt gctgctgctg ttcggccgcg acgtcgtcgt gtcgcgtatc gaggcggcgc tcgcgtaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa