ButhA.01181.a
Thymidylate synthase (TS) (TSase) (EC 2.1.1.45) (BTH_I1680) (thyA)
| CENTER ID: | ButhA.01181.a |
| ORGANISM: | Burkholderia thailandensis E264 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.01181.a.A1.GE32758 | full length | 1 | 323 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.01181.a.A1.PS01191 | full length | 1 | 323 |
Structures
3V8H
DEPOSITED: 12/22/2011
DETERMINATION: XRay
CLONE: ButhA.01181.a.A1.GE32758
PROTEIN: ButhA.01181.a.A1.PS01191
Publications by SSGCID
Combining functional and structural genomics to sample the essential Burkholderia structome.
Abendroth J, Armour B, Barrett L, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Fox D, Gallagher LA, Gardberg AS, Gillespie A, Manoil C, Myler PJ, Nakazawa-Hewitt S, Napuli A, Nguyen MT, Patrapuvich R, Phan I, Stacy R, Staker BL, Stewart LJ, Van Voorhis WC
PLoS ONE - 2012
volume 8, issue 1, pages e53851
PMID: 23382856; PMCID: PMC3561365
PDB:
3D63,
3DAH,
3EIZ,
3EJ2,
3EK2,
3EZO,
3F0F,
3FTP,
3GK0,
3GK3,
3GVF,
3GWA,
3GWE,
3IML,
3SZ8,
3T4C,
3TML,
3TMQ,
3TXY,
3U7J,
3UE9,
3UK1,
3UK2,
3UND,
3UPT,
3URR,
3UW1,
3UW2,
3UW3,
3V2I,
3V7N,
3V8H,
3V9O,
3V9P,
3VAV,
4DDO,
4DFE,
4DHE,
4DHK,
4DUT,
4DZ4,
4E4T,
4E51,
4EFI,
4EG0,
4EGJ,
4EK2,
4EQY,
4EWG,
4EX5,
4EXQ,
4F2G,
4F32,
4F3N,
4F3Y,
4F4H,
4F7D,
4FK8,
4FRY,
4G1K,
4G67,
4G6Z,
4GHK,
4H3Y,
4H3Z,
4H4G
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_127385 |
| PATRIC ID: | fig|271848.6.peg.4469 |
| RefSeq: | YP_442217.1 |
| UniProt: | Q2SXY2 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKQYLDLVRT ILDTGTWQSN RTGIRTIGIP GAMLRFDLQQ GFPAVTTKKL AFKSAIGELV GFLRATRSAA EFRALGCKVW DANANENAQW LANPYRRGAD DLGDVYGVQW RRWPGYKVLD AHADAQIADA TSRGFRIVAR FEEGGADKVL LHKAIDQLRD CLDTIVRDPS SRRILFHGWN PAVLDEIALP ACHLLYQFLP NVERREISLC LYIRSNDVGL GTPFNLAEGA ALLTLVGRLT GYSPRWFTYF IGDAHIYENQ LDMLKQQLER EPFESPRLEL AERVPDYAKT GKYEPQWLER VEPSDFTLVG YRHHEPLSAP MAV
NT Sequence
atgaaacagt atctcgatct cgttcgcacc attctcgaca ccggcacctg gcagagcaac cgcacgggca tccgcacgat cggcatcccg ggcgcgatgc tgcgcttcga tctgcaacag ggctttcccg ccgtgacgac gaagaagctc gcgttcaagt cggcgatcgg cgagctcgtc ggcttcctgc gcgcgacgcg cagcgcggcg gagtttcgcg cgctcggctg caaggtgtgg gacgcgaatg cgaatgagaa cgcgcagtgg ctcgcgaatc cgtatcgccg cggcgccgac gatctcggcg acgtgtacgg cgtccaatgg cggcgctggc cgggctacaa ggtgctcgac gcgcacgccg acgcgcagat cgccgatgcg acttcgcgcg gctttcgcat cgtcgcgcgc ttcgaggagg gcggcgcgga caaggtgctg ctccacaagg cgatcgatca actgcgcgat tgcctcgaca cgatcgtgcg cgatccgtcg agccggcgca tcctgttcca cggctggaat cccgcggtgc tcgacgagat cgcgctgccc gcatgccatc tgctgtatca gttcctgccg aacgtcgagc ggcgcgaaat ctcgctgtgc ctgtacatcc gcagcaacga cgtcggactc ggcacgccgt tcaatctcgc cgaaggcgcg gcgctcctca cgctcgtcgg ccggctgacg ggctactcgc cgcgctggtt cacgtacttt atcggcgatg cgcacatcta cgagaatcag ctcgacatgc tcaagcagca actcgagcgc gagccgttcg agagtccgcg gctcgagctt gccgagcgcg tgcccgatta cgcgaagacg ggcaagtacg agccgcaatg gctcgaacgc gtcgagccgt cggatttcac gctcgtcggc tatcgccatc atgaaccgct gagcgcgccg atggccgtct ga
Details for ButhA.01181.a.A1.GE32758
| HARVESTED ON: | 6/20/2011 |
| SEQUENCED ON: | 7/14/2011 |
| EXPECTED MW: | 38kDa |
| OBSERVED MW: | 38kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKQYLDLVR TILDTGTWQS NRTGIRTIGI PGAMLRFDLQ QGFPAVTTKK LAFKSAIGEL VGFLRATRSA AEFRALGCKV WDANANENAQ WLANPYRRGA DDLGDVYGVQ WRRWPGYKVL DAHADAQIAD ATSRGFRIVA RFEEGGADKV LLHKAIDQLR DCLDTIVRDP SSRRILFHGW NPAVLDEIAL PACHLLYQFL PNVERREISL CLYIRSNDVG LGTPFNLAEG AALLTLVGRL TGYSPRWFTY FIGDAHIYEN QLDMLKQQLE REPFESPRLE LAERVPDYAK TGKYEPQWLE RVEPSDFTLV GYRHHEPLSA PMAV
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaaac agtatctcga tctcgttcgc accattctcg acaccggcac ctggcagagc aaccgcacgg gcatccgcac gatcggcatc ccgggcgcga tgctgcgctt cgatctgcaa cagggctttc ccgccgtgac gacgaagaag ctcgcgttca agtcggcgat cggcgagctc gtcggcttcc tgcgcgcgac gcgcagcgcg gcggagtttc gcgcgctcgg ctgcaaggtg tgggacgcga atgcgaatga gaacgcgcag tggctcgcga atccgtatcg ccgcggcgcc gacgatctcg gcgacgtgta cggcgtccaa tggcggcgct ggccgggcta caaggtgctc gacgcgcacg ccgacgcgca gatcgccgat gcgacttcgc gcggctttcg catcgtcgcg cgcttcgagg agggcggcgc ggacaaggtg ctgctccaca aggcgatcga tcaactgcgc gattgcctcg acacgatcgt gcgcgatccg tcgagccggc gcatcctgtt ccacggctgg aatcccgcgg tgctcgacga gatcgcgctg cccgcatgcc atctgctgta tcagttcctg ccgaacgtcg agcggcgcga aatctcgctg tgcctgtaca tccgcagcaa cgacgtcgga ctcggcacgc cgttcaatct cgccgaaggc gcggcgctcc tcacgctcgt cggccggctg acgggctact cgccgcgctg gttcacgtac tttatcggcg atgcgcacat ctacgagaat cagctcgaca tgctcaagca gcaactcgag cgcgagccgt tcgagagtcc gcggctcgag cttgccgagc gcgtgcccga ttacgcgaag acgggcaagt acgagccgca atggctcgaa cgcgtcgagc cgtcggattt cacgctcgtc ggctatcgcc atcatgaacc gctgagcgcg ccgatggccg tctaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKQYLDLVR TILDTGTWQS NRTGIRTIGI PGAMLRFDLQ QGFPAVTTKK LAFKSAIGEL VGFLRATRSA AEFRALGCKV WDANANENAQ WLANPYRRGA DDLGDVYGVQ WRRWPGYKVL DAHADAQIAD ATSRGFRIVA RFEEGGADKV LLHKAIDQLR DCLDTIVRDP SSRRILFHGW NPAVLDEIAL PACHLLYQFL PNVERREISL CLYIRSNDVG LGTPFNLAEG AALLTLVGRL TGYSPRWFTY FIGDAHIYEN QLDMLKQQLE REPFESPRLE LAERVPDYAK TGKYEPQWLE RVEPSDFTLV GYRHHEPLSA PMAV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaacagtat ctcgatctcg ttcgcaccat tctcgacacc ggcacctggc agagcaaccg cacgggcatc cgcacgatcg gcatcccggg cgcgatgctg cgcttcgatc tgcaacaggg ctttcccgcc gtgacgacga agaagctcgc gttcaagtcg gcgatcggcg agctcgtcgg cttcctgcgc gcgacgcgca gcgcggcgga gtttcgcgcg ctcggctgca aggtgtggga cgcgaatgcg aatgagaacg cgcagtggct cgcgaatccg tatcgccgcg gcgccgacga tctcggcgac gtgtacggcg tccaatggcg gcgctggccg ggctacaagg tgctcgacgc gcacgccgac gcgcagatcg ccgatgcgac ttcgcgcggc tttcgcatcg tcgcgcgctt cgaggagggc ggcgcggaca aggtgctgct ccacaaggcg atcgatcaac tgcgcgattg cctcgacacg atcgtgcgcg atccgtcgag ccggcgcatc ctgttccacg gctggaatcc cgcggtgctc gacgagatcg cgctgcccgc atgccatctg ctgtatcagt tcctgccgaa cgtcgagcgg cgcgaaatct cgctgtgcct gtacatccgc agcaacgacg tcggactcgg cacgccgttc aatctcgccg aaggcgcggc gctcctcacg ctcgtcggcc ggctgacggg ctactcgccg cgctggttca cgtactttat cggcgatgcg cacatctacg agaatcagct cgacatgctc aagcagcaac tcgagcgcga gccgttcgag agtccgcggc tcgagcttgc cgagcgcgtg cccgattacg cgaagacggg caagtacgag ccgcaatggc tcgaacgcgt cgagccgtcg gatttcacgc tcgtcggcta tcgccatcat gaaccgctga gcgcgccgat ggccgtctaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for ButhA.01181.a.A1.PS01191
| PURIFICATION DATe: | 8/23/2011 |
| CONCENTRATION: | 51.93mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKQYLDLVR TILDTGTWQS NRTGIRTIGI PGAMLRFDLQ QGFPAVTTKK LAFKSAIGEL VGFLRATRSA AEFRALGCKV WDANANENAQ WLANPYRRGA DDLGDVYGVQ WRRWPGYKVL DAHADAQIAD ATSRGFRIVA RFEEGGADKV LLHKAIDQLR DCLDTIVRDP SSRRILFHGW NPAVLDEIAL PACHLLYQFL PNVERREISL CLYIRSNDVG LGTPFNLAEG AALLTLVGRL TGYSPRWFTY FIGDAHIYEN QLDMLKQQLE REPFESPRLE LAERVPDYAK TGKYEPQWLE RVEPSDFTLV GYRHHEPLSA PMAV
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaaac agtatctcga tctcgttcgc accattctcg acaccggcac ctggcagagc aaccgcacgg gcatccgcac gatcggcatc ccgggcgcga tgctgcgctt cgatctgcaa cagggctttc ccgccgtgac gacgaagaag ctcgcgttca agtcggcgat cggcgagctc gtcggcttcc tgcgcgcgac gcgcagcgcg gcggagtttc gcgcgctcgg ctgcaaggtg tgggacgcga atgcgaatga gaacgcgcag tggctcgcga atccgtatcg ccgcggcgcc gacgatctcg gcgacgtgta cggcgtccaa tggcggcgct ggccgggcta caaggtgctc gacgcgcacg ccgacgcgca gatcgccgat gcgacttcgc gcggctttcg catcgtcgcg cgcttcgagg agggcggcgc ggacaaggtg ctgctccaca aggcgatcga tcaactgcgc gattgcctcg acacgatcgt gcgcgatccg tcgagccggc gcatcctgtt ccacggctgg aatcccgcgg tgctcgacga gatcgcgctg cccgcatgcc atctgctgta tcagttcctg ccgaacgtcg agcggcgcga aatctcgctg tgcctgtaca tccgcagcaa cgacgtcgga ctcggcacgc cgttcaatct cgccgaaggc gcggcgctcc tcacgctcgt cggccggctg acgggctact cgccgcgctg gttcacgtac tttatcggcg atgcgcacat ctacgagaat cagctcgaca tgctcaagca gcaactcgag cgcgagccgt tcgagagtcc gcggctcgag cttgccgagc gcgtgcccga ttacgcgaag acgggcaagt acgagccgca atggctcgaa cgcgtcgagc cgtcggattt cacgctcgtc ggctatcgcc atcatgaacc gctgagcgcg ccgatggccg tctaataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKQYLDLVR TILDTGTWQS NRTGIRTIGI PGAMLRFDLQ QGFPAVTTKK LAFKSAIGEL VGFLRATRSA AEFRALGCKV WDANANENAQ WLANPYRRGA DDLGDVYGVQ WRRWPGYKVL DAHADAQIAD ATSRGFRIVA RFEEGGADKV LLHKAIDQLR DCLDTIVRDP SSRRILFHGW NPAVLDEIAL PACHLLYQFL PNVERREISL CLYIRSNDVG LGTPFNLAEG AALLTLVGRL TGYSPRWFTY FIGDAHIYEN QLDMLKQQLE REPFESPRLE LAERVPDYAK TGKYEPQWLE RVEPSDFTLV GYRHHEPLSA PMAV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAAACAGTAT CTCGATCTCG TTCGCACCAT TCTCGACACC GGCACCTGGC AGAGCAACCG CACGGGCATC CGCACGATCG GCATCCCGGG CGCGATGCTG CGCTTCGATC TGCAACAGGG CTTTCCCGCC GTGACGACGA AGAAGCTCGC GTTCAAGTCG GCGATCGGCG AGCTCGTCGG CTTCCTGCGC GCGACGCGCA GCGCGGCGGA GTTTCGCGCG CTCGGCTGCA AGGTGTGGGA CGCGAATGCG AATGAGAACG CGCAGTGGCT CGCGAATCCG TATCGCCGCG GCGCCGACGA TCTCGGCGAC GTGTACGGCG TCCAATGGCG GCGCTGGCCG GGCTACAAGG TGCTCGACGC GCACGCCGAC GCGCAGATCG CCGATGCGAC TTCGCGCGGC TTTCGCATCG TCGCGCGCTT CGAGGAGGGC GGCGCGGACA AGGTGCTGCT CCACAAGGCG ATCGATCAAC TGCGCGATTG CCTCGACACG ATCGTGCGCG ATCCGTCGAG CCGGCGCATC CTGTTCCACG GCTGGAATCC CGCGGTGCTC GACGAGATCG CGCTGCCCGC ATGCCATCTG CTGTATCAGT TCCTGCCGAA CGTCGAGCGG CGCGAAATCT CGCTGTGCCT GTACATCCGC AGCAACGACG TCGGACTCGG CACGCCGTTC AATCTCGCCG AAGGCGCGGC GCTCCTCACG CTCGTCGGCC GGCTGACGGG CTACTCGCCG CGCTGGTTCA CGTACTTTAT CGGCGATGCG CACATCTACG AGAATCAGCT CGACATGCTC AAGCAGCAAC TCGAGCGCGA GCCGTTCGAG AGTCCGCGGC TCGAGCTTGC CGAGCGCGTG CCCGATTACG CGAAGACGGG CAAGTACGAG CCGCAATGGC TCGAACGCGT CGAGCCGTCG GATTTCACGC TCGTCGGCTA TCGCCATCAT GAACCGCTGA GCGCGCCGAT GGCCGTCTAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA