ButhA.01152.a
Uroporphyrinogen decarboxylase (UPD) (URO-D) (EC 4.1.1.37) (BTH_I3304) (hemE)
| CENTER ID: | ButhA.01152.a |
| ORGANISM: | Burkholderia thailandensis E264 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.01152.a.A1.GE32815 | full length | 1 | 364 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.01152.a.A1.PW33419 | full length | 1 | 364 |
Structures
4EXQ
DEPOSITED: 4/30/2012
DETERMINATION: XRay
CLONE: ButhA.01152.a.A1.GE32815
PROTEIN: ButhA.01152.a.A1.PW33419
Publications by SSGCID
Combining functional and structural genomics to sample the essential Burkholderia structome.
Abendroth J, Armour B, Barrett L, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Fox D, Gallagher LA, Gardberg AS, Gillespie A, Manoil C, Myler PJ, Nakazawa-Hewitt S, Napuli A, Nguyen MT, Patrapuvich R, Phan I, Stacy R, Staker BL, Stewart LJ, Van Voorhis WC
PLoS ONE - 2012
volume 8, issue 1, pages e53851
PMID: 23382856; PMCID: PMC3561365
PDB:
3D63,
3DAH,
3EIZ,
3EJ2,
3EK2,
3EZO,
3F0F,
3FTP,
3GK0,
3GK3,
3GVF,
3GWA,
3GWE,
3IML,
3SZ8,
3T4C,
3TML,
3TMQ,
3TXY,
3U7J,
3UE9,
3UK1,
3UK2,
3UND,
3UPT,
3URR,
3UW1,
3UW2,
3UW3,
3V2I,
3V7N,
3V8H,
3V9O,
3V9P,
3VAV,
4DDO,
4DFE,
4DHE,
4DHK,
4DUT,
4DZ4,
4E4T,
4E51,
4EFI,
4EG0,
4EGJ,
4EK2,
4EQY,
4EWG,
4EX5,
4EXQ,
4F2G,
4F32,
4F3N,
4F3Y,
4F4H,
4F7D,
4FK8,
4FRY,
4G1K,
4G67,
4G6Z,
4GHK,
4H3Y,
4H3Z,
4H4G
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_127347 |
| PATRIC ID: | fig|271848.6.peg.6191 |
| RefSeq: | YP_443796.1 |
| UniProt: | Q2STF3 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAQTLINDTF LRALLREPTD YTPIWLMRQA GRYLPEYNAT RARAGSFLGL AKHPDYATEV TLQPLERFPL DAAILFSDIL TIPDAMGLGL DFAAGEGPKF AHPVRTEADV AKLAVPDIGA TLGYVTDAVR EIRRALTDGE GRQRVPLIGF SGSPWTLACY MVEGGGSDDF RTVKSMAYAR PDLMHRILDV NAQAVAAYLN AQIEAGAQAV MIFDTWGGAL ADGAYQRFSL DYIRRVVAQL KREHDGARVP AIAFTKGGGL WLEDLAATGV DAVGLDWTVN LGRARERVAG RVALQGNLDP TILFAPPEAI RAEARAVLDS YGNHPGHVFN LGHGISQFTP PEHVAELVDE VHRHSRAIRS GTGS
NT Sequence
gtggcccaga ctctcatcaa cgacaccttc ctgcgcgcgc tgctgcgcga gccgaccgac tacacgccga tctggctgat gcgccaggcg ggccgctacc tgcccgagta caacgccacg cgcgcgcgcg ccggcagctt tctcgggctc gcgaagcatc ccgattacgc gaccgaggtg acgctgcagc cgctcgagcg cttcccgctc gacgccgcga tcctgttttc cgacatcctg acgattcccg acgcgatggg gctcggcctc gatttcgccg cgggcgaggg gccgaagttc gcgcatccgg tgcgcaccga ggccgatgtc gcgaagctcg cggtgccgga catcggcgcg acgctcggct acgtgaccga cgcggtgcga gagattcgcc gcgcgctcac cgacggcgaa ggtcgccagc gtgtgccgct gatcggtttc tccggcagcc cgtggacgct cgcgtgctac atggtggaag gcggcggctc ggacgatttc cgcacggtga agtcgatggc gtatgcgcgg cctgacctga tgcaccgcat tctcgacgtg aacgcgcagg cagtggccgc atacctgaac gcgcagatcg aagccggcgc gcaggccgtg atgatcttcg atacctgggg aggcgcgctg gccgacggcg cgtatcagcg cttctcgctc gactacatcc ggcgcgtcgt cgcgcagttg aaacgcgagc acgacggcgc gcgcgtgccg gcgatcgcgt tcacgaaggg cggcgggctc tggctcgaag acttggcggc gacgggcgtc gacgcggtcg gcctcgactg gacggtcaac ctcggccgcg cgcgcgagcg cgtcgcgggg cgcgtcgcgc tgcagggcaa cctcgatccg acgatcctgt tcgcgccgcc cgaggcgatt cgcgccgagg cgcgcgcggt gctcgacagc tacggcaatc acccgggcca cgtgttcaac ctgggccacg gcatttcgca gttcacgccg cccgagcacg ttgcggagct cgtagacgaa gtgcatcgcc atagccgcgc gatccggagc ggaaccggat cgtaa
Details for ButhA.01152.a.A1.GE32815
| HARVESTED ON: | 6/28/2011 |
| SEQUENCED ON: | 7/22/2011 |
| EXPECTED MW: | 41kDa |
| OBSERVED MW: | 41kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 94 |
Validated AA Sequence
MVAQTLINDT FLRALLREPT DYTPIWLMRQ AGRYLPEYNA TRARAGSFLG LAKHPDYATE VTLQPLERFP LDAAILFSDI LTIPDAMGLG LDFAAGEGPK FAHPVRTEAD VAKLAVPDIG ATLGYVTDAV REIRRALTDG EGRQRVPLIG FSGSPWTLAC YMVEGGGSDD FRTVKSMAYA RPDLMHRILD VNAQAVAAYL NAQIEAGAQA VMIFDTWGGA LADGAYQRFS LDYIRRVVAQ LKREHDGARV PAIAFTKGGG LWLEDLAATG VDAVGLDWTV NLGRARERVA GRVALQGNLD PTILFAPPEA IRAEARAVLD SYGNHPGHVF NLGHGISQFT PPEHVAELVD EVHRHSRAIR SGTGS
Validated NT Sequence
ttattacgat ccggttccgc tccggatcgc gcggctatgg cgatgcactt cgtctacgag ctccgcaacg tgctcgggcg gcgtgaactg cgaaatgccg tggcccaggt tgaacacgtg gcccgggtga ttgccgtagc tgtcgagcac cgcgcgcgcc tcggcgcgaa tcgcctcggg cggcgcgaac aggatcgtcg gatcgaggtt gccctgcagc gcgacgcgcc ccgcgacgcg ctcgcgcgcg cggccgaggt tgaccgtcca gtcgaggccg accgcgtcga cgcccgtcgc cgccaagtct tcgagccaga gcccgccgcc cttcgtgaac gcgatcgccg gcacgcgcgc gccgtcgtgc tcgcgtttca actgcgcgac gacgcgccgg atgtagtcga gcgagaagcg ctgatacgcg ccgtcggcca gcgcgcctcc ccaggtatcg aagatcatca cggcctgcgc gccggcttcg atctgcgcgt tcaggtatgc ggccactgcc tgcgcgttca cgtcgagaat gcggtgcatc aggtcaggcc gcgcatacgc catcgacttc accgtgcgga aatcgtccga gccgccgcct tccaccatgt agcacgcgag cgtccacggg ctgccggaga aaccgatcag cggcacacgc tggcgacctt cgccgtcggt gagcgcgcgg cgaatctctc gcaccgcgtc ggtcacgtag ccgagcgtcg cgccgatgtc cggcaccgcg agcttcgcga catcggcctc ggtgcgcacc ggatgcgcga acttcggccc ctcgcccgcg gcgaaatcga ggccgagccc catcgcgtcg ggaatcgtca ggatgtcgga aaacaggatc gcggcgtcga gcgggaagcg ctcgagcggc tgcagcgtca cctcggtcgc gtaatcggga tgcttcgcga gcccgagaaa gctgccggcg cgcgcgcgcg tggcgttgta ctcgggcagg tagcggcccg cctggcgcat cagccagatc ggcgtgtagt cggtcggctc gcgcagcagc gcgcgcagga aggtgtcgtt gatgagagtc tgggccacca tcgaaccagg accctgggtc tgagcttcca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVAQTLIND TFLRALLREP TDYTPIWLMR QAGRYLPEYN ATRARAGSFL GLAKHPDYAT EVTLQPLERF PLDAAILFSD ILTIPDAMGL GLDFAAGEGP KFAHPVRTEA DVAKLAVPDI GATLGYVTDA VREIRRALTD GEGRQRVPLI GFSGSPWTLA CYMVEGGGSD DFRTVKSMAY ARPDLMHRIL DVNAQAVAAY LNAQIEAGAQ AVMIFDTWGG ALADGAYQRF SLDYIRRVVA QLKREHDGAR VPAIAFTKGG GLWLEDLAAT GVDAVGLDWT VNLGRARERV AGRVALQGNL DPTILFAPPE AIRAEARAVL DSYGNHPGHV FNLGHGISQF TPPEHVAELV DEVHRHSRAI RSGTGS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggcccag actctcatca acgacacctt cctgcgcgcg ctgctgcgcg agccgaccga ctacacgccg atctggctga tgcgccaggc gggccgctac ctgcccgagt acaacgccac gcgcgcgcgc gccggcagct ttctcgggct cgcgaagcat cccgattacg cgaccgaggt gacgctgcag ccgctcgagc gcttcccgct cgacgccgcg atcctgtttt ccgacatcct gacgattccc gacgcgatgg ggctcggcct cgatttcgcc gcgggcgagg ggccgaagtt cgcgcatccg gtgcgcaccg aggccgatgt cgcgaagctc gcggtgccgg acatcggcgc gacgctcggc tacgtgaccg acgcggtgcg agagattcgc cgcgcgctca ccgacggcga aggtcgccag cgtgtgccgc tgatcggttt ctccggcagc ccgtggacgc tcgcgtgcta catggtggaa ggcggcggct cggacgattt ccgcacggtg aagtcgatgg cgtatgcgcg gcctgacctg atgcaccgca ttctcgacgt gaacgcgcag gcagtggccg catacctgaa cgcgcagatc gaagccggcg cgcaggccgt gatgatcttc gatacctggg gaggcgcgct ggccgacggc gcgtatcagc gcttctcgct cgactacatc cggcgcgtcg tcgcgcagtt gaaacgcgag cacgacggcg cgcgcgtgcc ggcgatcgcg ttcacgaagg gcggcgggct ctggctcgaa gacttggcgg cgacgggcgt cgacgcggtc ggcctcgact ggacggtcaa cctcggccgc gcgcgcgagc gcgtcgcggg gcgcgtcgcg ctgcagggca acctcgatcc gacgatcctg ttcgcgccgc ccgaggcgat tcgcgccgag gcgcgcgcgg tgctcgacag ctacggcaat cacccgggcc acgtgttcaa cctgggccac ggcatttcgc agttcacgcc gcccgagcac gttgcggagc tcgtagacga agtgcatcgc catagccgcg cgatccggag cggaaccgga tcgtaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for ButhA.01152.a.A1.PW33419
| PURIFICATION DATe: | 9/23/2011 |
| CONCENTRATION: | 25.27mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 11 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 94 |
Protocol Notes
notes unavailable
Validated AA Sequence
MVAQTLINDT FLRALLREPT DYTPIWLMRQ AGRYLPEYNA TRARAGSFLG LAKHPDYATE VTLQPLERFP LDAAILFSDI LTIPDAMGLG LDFAAGEGPK FAHPVRTEAD VAKLAVPDIG ATLGYVTDAV REIRRALTDG EGRQRVPLIG FSGSPWTLAC YMVEGGGSDD FRTVKSMAYA RPDLMHRILD VNAQAVAAYL NAQIEAGAQA VMIFDTWGGA LADGAYQRFS LDYIRRVVAQ LKREHDGARV PAIAFTKGGG LWLEDLAATG VDAVGLDWTV NLGRARERVA GRVALQGNLD PTILFAPPEA IRAEARAVLD SYGNHPGHVF NLGHGISQFT PPEHVAELVD EVHRHSRAIR SGTGS
Validated NT Sequence
ttattacgat ccggttccgc tccggatcgc gcggctatgg cgatgcactt cgtctacgag ctccgcaacg tgctcgggcg gcgtgaactg cgaaatgccg tggcccaggt tgaacacgtg gcccgggtga ttgccgtagc tgtcgagcac cgcgcgcgcc tcggcgcgaa tcgcctcggg cggcgcgaac aggatcgtcg gatcgaggtt gccctgcagc gcgacgcgcc ccgcgacgcg ctcgcgcgcg cggccgaggt tgaccgtcca gtcgaggccg accgcgtcga cgcccgtcgc cgccaagtct tcgagccaga gcccgccgcc cttcgtgaac gcgatcgccg gcacgcgcgc gccgtcgtgc tcgcgtttca actgcgcgac gacgcgccgg atgtagtcga gcgagaagcg ctgatacgcg ccgtcggcca gcgcgcctcc ccaggtatcg aagatcatca cggcctgcgc gccggcttcg atctgcgcgt tcaggtatgc ggccactgcc tgcgcgttca cgtcgagaat gcggtgcatc aggtcaggcc gcgcatacgc catcgacttc accgtgcgga aatcgtccga gccgccgcct tccaccatgt agcacgcgag cgtccacggg ctgccggaga aaccgatcag cggcacacgc tggcgacctt cgccgtcggt gagcgcgcgg cgaatctctc gcaccgcgtc ggtcacgtag ccgagcgtcg cgccgatgtc cggcaccgcg agcttcgcga catcggcctc ggtgcgcacc ggatgcgcga acttcggccc ctcgcccgcg gcgaaatcga ggccgagccc catcgcgtcg ggaatcgtca ggatgtcgga aaacaggatc gcggcgtcga gcgggaagcg ctcgagcggc tgcagcgtca cctcggtcgc gtaatcggga tgcttcgcga gcccgagaaa gctgccggcg cgcgcgcgcg tggcgttgta ctcgggcagg tagcggcccg cctggcgcat cagccagatc ggcgtgtagt cggtcggctc gcgcagcagc gcgcgcagga aggtgtcgtt gatgagagtc tgggccacca tcgaaccagg accctgggtc tgagcttcca
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVAQTLIND TFLRALLREP TDYTPIWLMR QAGRYLPEYN ATRARAGSFL GLAKHPDYAT EVTLQPLERF PLDAAILFSD ILTIPDAMGL GLDFAAGEGP KFAHPVRTEA DVAKLAVPDI GATLGYVTDA VREIRRALTD GEGRQRVPLI GFSGSPWTLA CYMVEGGGSD DFRTVKSMAY ARPDLMHRIL DVNAQAVAAY LNAQIEAGAQ AVMIFDTWGG ALADGAYQRF SLDYIRRVVA QLKREHDGAR VPAIAFTKGG GLWLEDLAAT GVDAVGLDWT VNLGRARERV AGRVALQGNL DPTILFAPPE AIRAEARAVL DSYGNHPGHV FNLGHGISQF TPPEHVAELV DEVHRHSRAI RSGTGS
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGGCCCAG ACTCTCATCA ACGACACCTT CCTGCGCGCG CTGCTGCGCG AGCCGACCGA CTACACGCCG ATCTGGCTGA TGCGCCAGGC GGGCCGCTAC CTGCCCGAGT ACAACGCCAC GCGCGCGCGC GCCGGCAGCT TTCTCGGGCT CGCGAAGCAT CCCGATTACG CGACCGAGGT GACGCTGCAG CCGCTCGAGC GCTTCCCGCT CGACGCCGCG ATCCTGTTTT CCGACATCCT GACGATTCCC GACGCGATGG GGCTCGGCCT CGATTTCGCC GCGGGCGAGG GGCCGAAGTT CGCGCATCCG GTGCGCACCG AGGCCGATGT CGCGAAGCTC GCGGTGCCGG ACATCGGCGC GACGCTCGGC TACGTGACCG ACGCGGTGCG AGAGATTCGC CGCGCGCTCA CCGACGGCGA AGGTCGCCAG CGTGTGCCGC TGATCGGTTT CTCCGGCAGC CCGTGGACGC TCGCGTGCTA CATGGTGGAA GGCGGCGGCT CGGACGATTT CCGCACGGTG AAGTCGATGG CGTATGCGCG GCCTGACCTG ATGCACCGCA TTCTCGACGT GAACGCGCAG GCAGTGGCCG CATACCTGAA CGCGCAGATC GAAGCCGGCG CGCAGGCCGT GATGATCTTC GATACCTGGG GAGGCGCGCT GGCCGACGGC GCGTATCAGC GCTTCTCGCT CGACTACATC CGGCGCGTCG TCGCGCAGTT GAAACGCGAG CACGACGGCG CGCGCGTGCC GGCGATCGCG TTCACGAAGG GCGGCGGGCT CTGGCTCGAA GACTTGGCGG CGACGGGCGT CGACGCGGTC GGCCTCGACT GGACGGTCAA CCTCGGCCGC GCGCGCGAGC GCGTCGCGGG GCGCGTCGCG CTGCAGGGCA ACCTCGATCC GACGATCCTG TTCGCGCCGC CCGAGGCGAT TCGCGCCGAG GCGCGCGCGG TGCTCGACAG CTACGGCAAT CACCCGGGCC ACGTGTTCAA CCTGGGCCAC GGCATTTCGC AGTTCACGCC GCCCGAGCAC GTTGCGGAGC TCGTAGACGA AGTGCATCGC CATAGCCGCG CGATCCGGAG CGGAACCGGA TCGTAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA