ButhA.01152.a
Uroporphyrinogen decarboxylase (UPD) (URO-D) (EC 4.1.1.37) (BTH_I3304) (hemE)
CENTER ID:
ButhA.01152.a
ORGANISM:
Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:
in PDB
COMMUNITY REQUEST:
True
NIH RISK GROUP:
3
SELECT AGENT:
False
NIH PRIORITY
pathogens category:
IIIB
Ordering Clones & Proteins
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Clones*
CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO
AA
START
AA
STOP
ORDER
MATERIAL
ButhA.01152.a.A1.GE32815
full length
info
1
364
ButhA.01152.a.A1.GE32815
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis . Get sequence information
using the button in the "info" column.
Proteins
CENTER REFERENCE ID
DOMAIN/REGION DESCRIPTION
INFO
AA START
AA STOP
ORDER MATERIAL
ButhA.01152.a.A1.PW33419
full length
info
1
364
PW33419
Structures
4EXQ
DEPOSITED: 4/30/2012
DETERMINATION: XRay
CLONE: ButhA.01152.a.A1.GE32815
PROTEIN: ButhA.01152.a.A1.PW33419
Publications by SSGCID
Combining functional and structural genomics to sample the essential Burkholderia structome.
Abendroth J, Armour B, Barrett L, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Fox D, Gallagher LA, Gardberg AS, Gillespie A, Manoil C, Myler PJ, Nakazawa-Hewitt S, Napuli A, Nguyen MT, Patrapuvich R, Phan I, Stacy R, Staker BL, Stewart LJ, Van Voorhis WC
PLoS ONE - 2012
volume 8, issue 1, pages e53851
PDB:
3D63 ,
3DAH ,
3EIZ ,
3EJ2 ,
3EK2 ,
3EZO ,
3F0F ,
3FTP ,
3GK0 ,
3GK3 ,
3GVF ,
3GWA ,
3GWE ,
3IML ,
3SZ8 ,
3T4C ,
3TML ,
3TMQ ,
3TXY ,
3U7J ,
3UE9 ,
3UK1 ,
3UK2 ,
3UND ,
3UPT ,
3URR ,
3UW1 ,
3UW2 ,
3UW3 ,
3V2I ,
3V7N ,
3V8H ,
3V9O ,
3V9P ,
3VAV ,
4DDO ,
4DFE ,
4DHE ,
4DHK ,
4DUT ,
4DZ4 ,
4E4T ,
4E51 ,
4EFI ,
4EG0 ,
4EGJ ,
4EK2 ,
4EQY ,
4EWG ,
4EX5 ,
4EXQ ,
4F2G ,
4F32 ,
4F3N ,
4F3Y ,
4F4H ,
4F7D ,
4FK8 ,
4FRY ,
4G1K ,
4G67 ,
4G6Z ,
4GHK ,
4H3Y ,
4H3Z ,
4H4G
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAQTLINDTF LRALLREPTD YTPIWLMRQA GRYLPEYNAT RARAGSFLGL AKHPDYATEV TLQPLERFPL DAAILFSDIL TIPDAMGLGL DFAAGEGPKF AHPVRTEADV AKLAVPDIGA TLGYVTDAVR EIRRALTDGE GRQRVPLIGF SGSPWTLACY MVEGGGSDDF RTVKSMAYAR PDLMHRILDV NAQAVAAYLN AQIEAGAQAV MIFDTWGGAL ADGAYQRFSL DYIRRVVAQL KREHDGARVP AIAFTKGGGL WLEDLAATGV DAVGLDWTVN LGRARERVAG RVALQGNLDP TILFAPPEAI RAEARAVLDS YGNHPGHVFN LGHGISQFTP PEHVAELVDE VHRHSRAIRS GTGS
NT Sequence
gtggcccaga ctctcatcaa cgacaccttc ctgcgcgcgc tgctgcgcga gccgaccgac tacacgccga tctggctgat gcgccaggcg ggccgctacc tgcccgagta caacgccacg cgcgcgcgcg ccggcagctt tctcgggctc gcgaagcatc ccgattacgc gaccgaggtg acgctgcagc cgctcgagcg cttcccgctc gacgccgcga tcctgttttc cgacatcctg acgattcccg acgcgatggg gctcggcctc gatttcgccg cgggcgaggg gccgaagttc gcgcatccgg tgcgcaccga ggccgatgtc gcgaagctcg cggtgccgga catcggcgcg acgctcggct acgtgaccga cgcggtgcga gagattcgcc gcgcgctcac cgacggcgaa ggtcgccagc gtgtgccgct gatcggtttc tccggcagcc cgtggacgct cgcgtgctac atggtggaag gcggcggctc ggacgatttc cgcacggtga agtcgatggc gtatgcgcgg cctgacctga tgcaccgcat tctcgacgtg aacgcgcagg cagtggccgc atacctgaac gcgcagatcg aagccggcgc gcaggccgtg atgatcttcg atacctgggg aggcgcgctg gccgacggcg cgtatcagcg cttctcgctc gactacatcc ggcgcgtcgt cgcgcagttg aaacgcgagc acgacggcgc gcgcgtgccg gcgatcgcgt tcacgaaggg cggcgggctc tggctcgaag acttggcggc gacgggcgtc gacgcggtcg gcctcgactg gacggtcaac ctcggccgcg cgcgcgagcg cgtcgcgggg cgcgtcgcgc tgcagggcaa cctcgatccg acgatcctgt tcgcgccgcc cgaggcgatt cgcgccgagg cgcgcgcggt gctcgacagc tacggcaatc acccgggcca cgtgttcaac ctgggccacg gcatttcgca gttcacgccg cccgagcacg ttgcggagct cgtagacgaa gtgcatcgcc atagccgcgc gatccggagc ggaaccggat cgtaa
Details for ButhA.01152.a.A1.GE32815
HARVESTED ON:
6/28/2011
SEQUENCED ON:
7/22/2011
EXPECTED MW:
41kDa
OBSERVED MW:
41kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Too many to count (100+)
TOTAL EXPRESSION LEVEL:
Moderate Expression
SOLUBLE EXPRESSION LEVEL
Moderate Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
pass with incomplete coverage
PERCENT IDENTITY:
100
PERCENT COVERAGE:
94
Details for ButhA.01152.a.A1.PW33419
PURIFICATION DATe:
9/23/2011
CONCENTRATION:
25.27mg/ml
OBSERVED MW:
data unavailable
EXPRESSION LEVEL:
Moderate Expression
PROTEIN PURIFICATION BUFFER:
25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
11
VIAL VOLUME:
200µl
PERCENT IDENTITY:
100
PERCENT COVERAGE:
94